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Developmental regulation of PSD-95 and nNOS expression in lumbar spinal cord of rats

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Developmental regulation of PSD-95 and nNOS expression in lu...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Shang-Feng GAO~(1 2) Ai-Guo SHEN~2 Chun CHENG~(2 *) 1 Department of Neurobiology Institute for Neuroscience Zhejiang university school of Medicine Hangzhou 310058 China 2 Department of Microbiology and Immunology medical school of nantong university nantong 226001 China

＜正＞Postsynaptic density（PSD）-95 is originally isolated from glutamatergic synapse where it serves as a physical tether to allow neuronal nitric oxide synthase（nNOS） signaling by N-methyl-D-aspartate receptor（NMDAR） activity. Considering the physiological importance of glutamate receptor and nitric oxide（NO） during development,we examined the spatiotemporal expression of PSD-95 and nNOS in the lumbar spinal cord at a postnatal ***,both gene and protein levels of them gradually increased with age after birth,peaked at the postnatal day 14（P14）,and then decreased to an adult *** addition,the enhanced coimmunoprecipitations between PSD-95 and nNOS were detected in developing spinal ***,PSD-95 staining codistributed with nNOS in NeuN-positive motor neurons and sensory neurons at *** findings indicate that PSD-95 and nNOS might collectively participate in spinal cord development.

关键词： post-synaptic density-95 neuronal nitric oxide synthase postnatal development spinal cord rat coimmunoprecipitation immunofluorescence

Overexpression of Dyrkl A regulates SRp55-modulated tau alternative splicing

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Overexpression of Dyrkl A regulates SRp55-modulated tau alte...

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Xiaomin Yin~1,Jianhua Shi~1,Wei Qian~1,Nana Jin~2,Fei Liu~(2,3) 1.Department of biochemistry,medical school,nantong university,nantong,Jiangsu 226001,China 2.Jiangsu Key Laboratory of Neuroregeneration,nantong university 3.New York State Institute for Basic Research in Developmental Disability,Staten Island,New York 10314,USA

Background:Microtubule-associated protein tau plays important role in polymerization and stabilization of neuronal *** adult human brain expresses equal levels of tau with three microtubule-binding repeats(3R) or four repeats(4R),which are regulated by alternative splicing of tau exon 10(E10) that codes the second microtubule binding *** specific mutations of tau gene cause the shift of the ratio of 3R/4R tau by dysregulation of E10 alternative splicing,leading to frontotemporal dementia with Parkinsonism linked to chromosome 17(FTDP-17).Recently,we have found that overexpression of Dyrk1A(dual-specificity tyrosine phosphorylation-regulated kinase 1A) due to trisomy 21 in DS brain is highly related to the increased ***55,a member of serine/arignine rich (SR) protein family of splicing factors,was found to play a role in the alternative splicing of tau E10 in many ***:We applied GST-pull down,co-immunoprecipitation and co-localization strategies to study the interaction between SRp55 and *** minigene SI9/SI10 was selected to mimic tau splicing in this ***:We found that Dyrk1A interacts with SRp55 in vitro and in situ through SR2 domain of *** of Dyrk1A alters SRp55-regulated tau E10 splicing in cultured HEK-293T *** also observed that Dyrk1A phosphorylated SRp55 in an enzyme-concentration dependent manner in ***:SRp55 interacts with Dyrk1A and its RS2 sub-domain might mediate the *** phosphorylation by Dyrk1A may modulate SRp55-regulated alternative splicing of tau E10.

关键词： tau exon 10 SRp55 Dyrk1A

The effects of ngf on the proliferation and differentiation of endogenous nscs in the hippocampus into neurons after fimbria fornix transection

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The effects of ngf on the proliferation and differentiation ...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Chuan-Ming DONG,Guo-Hua JIN~*,Jian-Bing QIN,Mei-Ling TIAN,Lin-Qing ZOU,Xue-Feng TAN,Hui-Xia ZHU Department of Anatomy,Department of Biochemistry,Institute of Neurobiolog,Key lab of Neuroregeneration of Jiangsu Province,medical school,nantong university,nantong 226001

＜正＞Objective To study the effects of fimbria fornix transection and intraventricular injection of nerve growth factor （NGF） on the proliferation and differentiation of endogenous neural stem cells（NSCs）in the hippocampus into neurons. Methods Twelve SD rats were divided into the treatment group and control group randomly,six rats in each *** right fimbria fornix of rats were *** and artificial cerebrospinal fluid was injected into the lateral ventricle of rats in the treatment group and control group respectively immediately and on the 2nd and 4th day after *** （BrdU） was injected intraperitoneally twice per day from 3 to 7 d after *** the 28th day after operation,the brains were perfused for fixation and frozen ***/NF-200 double-label immunofluorescence was used to detect the proliferatiation and differentiatiation of NSCs into *** In dentate gyrus,the number of BrdU positive cells in the transection side were much more than those of normal side both in the treatment group and the control group,and the BrdU positive cells in treatment group were more than those of control group both in the transection side and normal side;The number of BrdU/NF-200 double labeled neurons was the most in the transection side of the treatment group,less in normal side of treatment group and transection side of the control group,but absent in the normal side of the control group. Conclusion Fimbria fornix transection could cause endogenous neural stem cells in the hippocampus proliferate and differentiate into neurons,and intraventricular injection of NGF could facilitate its proliferation and differentiation.

关键词： nerve growth factor(NGF) fimbria fornix transection hippocampus neural stem cells(NSCs) proliferation differentiation immunofluorescence rat

麋鹿角乙醇提取液对实验性衰老模型小鼠认知功能衰退的改善

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中国新药与临床杂志 2009年第7期28卷 505-508页

作者：秦红兵杨朝晔成海龙朱清盐城卫生职业技术学院江苏盐城224005 南通大学医学院药理学教研室江苏南通226001

目的观察麋鹿角乙醇提取液(EAEF)对D-半乳糖(D-gal)诱导衰老模型小鼠认知功能衰退的影响。方法ICR小鼠60只,随机分成4组:正常对照组(NG),模型对照组(MG),EAEF低剂量组(LG,2g·kg-1·d-1)及高剂量组(HG,4g·kg-1·d-1)。使用D-gal按120mg... 详细信息

目的观察麋鹿角乙醇提取液(EAEF)对D-半乳糖(D-gal)诱导衰老模型小鼠认知功能衰退的影响。方法ICR小鼠60只,随机分成4组:正常对照组(NG),模型对照组(MG),EAEF低剂量组(LG,2g·kg-1·d-1)及高剂量组(HG,4g·kg-1·d-1)。使用D-gal按120mg·kg-1给小鼠背部sc连续6wk,造成亚急性衰老模型。造模同时,LG和HG分别ig相应浓度的EAEF,于wk7用Y型电迷宫检测小鼠学习、记忆功能,并测定小鼠脑中脂褐质(LP)含量、单胺氧化酶-B(MAO-B)活性。结果与NG比较,MG认知功能明显下降,表现为正确反应潜伏期延长,正确反应率降低(P<0.05),而EAEF能明显缩短衰老小鼠潜伏期,减少连续3次正确所需次数,显著升高正确反应率(P<0.05或P<0.01)。生化检测指标显示,MG脑组织中LP含量升高(P<0.05);EAEF能降低脑组织中MAO-B活性以及脑组织中LP含量(P<0.05)。结论EAEF对D-gal诱导衰老模型小鼠的认知功能衰退有改善作用。

关键词：中草药衰老半乳糖认知麋鹿角

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灵光独耀:沈宗灵与中国法理学

中国法律评论 2024年第4期 149-154页

作者： Peking university Law school 北京大学法学院

在其诸多弟子的追忆中,沈宗灵先生一生专心治学、淡泊名利,由此常常对世事保持一种有意的疏离。“灵光独耀,迥脱根尘”,或许便是这位法理学大家的灵魂写照。壹“经国济民”:沈宗灵的早年岁月1923年2月24日,沈宗灵出生于浙江杭州的一个... 详细信息

在其诸多弟子的追忆中,沈宗灵先生一生专心治学、淡泊名利,由此常常对世事保持一种有意的疏离。“灵光独耀,迥脱根尘”,或许便是这位法理学大家的灵魂写照。壹“经国济民”:沈宗灵的早年岁月1923年2月24日,沈宗灵出生于浙江杭州的一个商人家庭,后随家人迁居上海。

关键词：沈宗灵淡泊名利法理学浙江杭州灵光

大鼠额叶损伤后细胞凋亡及iNOS的表达变化

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苏州大学学报（医学版） 2008年第5期28卷 747-749,882页

作者：何志贤董玉林张志军郁晓燕倪衡建南通大学医学院外总教研室江苏南通226001 南通大学医学院人体解剖学教研室江苏南通226001

目的探讨大鼠额叶锐器损伤后细胞凋亡的变化规律及可能机制。方法采用大鼠额叶锐器损伤模型,用TUNEL法检测细胞凋亡,用RT-PCR和Western blot检测诱导性一氧化氮合酶(iNOS)基因和蛋白的表达变化。结果凋亡细胞在损伤后3h即可发现,24h达... 详细信息

目的探讨大鼠额叶锐器损伤后细胞凋亡的变化规律及可能机制。方法采用大鼠额叶锐器损伤模型,用TUNEL法检测细胞凋亡,用RT-PCR和Western blot检测诱导性一氧化氮合酶(iNOS)基因和蛋白的表达变化。结果凋亡细胞在损伤后3h即可发现,24h达到高峰,随后逐渐减少;伤后3hiNOS基因和蛋白的表达开始上升,24h达到高峰,以后也逐渐下降。结论大鼠额叶锐器伤后存在细胞凋亡,凋亡细胞数量的变化与伤后时程有关,iNOS表达增加可能是影响细胞凋亡的重要因素。

关键词：额叶损伤凋亡诱导性一氧化氮合酶大鼠

Phosphorylation of SC35 regulates the alternative splicing of tau exon 10

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Phosphorylation of SC35 regulates the alternative splicing o...

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Wei Qian~2,Weihua Wang~1,Xiaomin Yin~2,Cheng-Xin Gong~3,Fei Liu~(1,2,3) 1.Jiangsu Key Laboratory of Neuroregeneration 2.Department of Biochemistry,medical school,nantong university,Jiangsu 226001,P.R.China 3.Department of Neurochemistry,New York State Institute for Basic Research in Developmental Disabilities,Staten Island,NY 10314, USA.

Background:Abnormal alternative splicing of tau exon 10 results in imbalance of 3R-tau and 4R-tau expression,which is sufficient to cause neurofibrillary *** factor SC35,a member of the superfamily of the serine/arginine-rich(SR) proteins,promotes tau exon 10 *** function of SC35 is tightly regulated by *** of DyrklA(dual-specificity tyrosine-phosphorylated and regulated kinase 1A) due to trisome in Down syndrome brain may contribute to the dysregulation of tau exon 10 ***1(protein phosphatase 1) dysregulated in Alzheimer’s desease brain is an abundant neuronal serine/threoning ***:By mutation of mini-tau gene,pCI/SI9-SI10 which consists of exons 9,10,11,a part of intron 9 and intron 10 and RNA co-immunoprecipitation(RNA-IP),we elucidated the molecular mechanism by which SC35 works on tau exon 10 splicing and whether and how Dyrk1A or PP1 regulates SC35-promoted tau exon 10 inclusion in vitro and in cultured ***:We observed that tau pre-mRNA could be precipitated by anti-SC35 *** of the SC35-like exonic splicing enhancer located at exon 10 of tau affected both the binding of SC35 to tau pre-mRNA and promotion of tau exon 10 inclusion,suggesting that SC35 acts on the SC35-like exonic splicing enhancer to promote tau exon 10 ***1A interacted with and phosphorylated *** affected the interaction between SC35 and tau pre-mRNA and suppressed SC35-promoted tau exon 10 ***1 also interacted with SC35 and downregulated the tau exon 10 ***:The phosphorylation of SC35 is regulated by DyrklA or PP1,which may cause dysregulation of tau exon 10 splicing,leading to the imbalance in 3R-tau and 4R-tau expression,which may initiate or accelerate tau pathology.

关键词： tau SC35 alternative splicing

Chinese Clinical Practice Guidelines for the Prevention and Treatment of Mother-to-Child Transmission of Hepatitis B Virus(Version 2024)

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Infectious Microbes & Diseases 2024年第3期6卷 108-116页

作者： Jinfeng Liu Qinglei Zeng Fanpu Ji Hong Ren Wenhong Zhang Lanjuan Li Yingren Zhao Infectious Diseases Physicians Branch of Chinese medical Doctor Association Chinese Society of Infectious Diseases of Chinese medical Association The First Affiliated Hospital of Xi’an Jiaotong university Xi’anChina Shaanxi Clinical medical Research Center of Infectious Diseases Xi’anChina The First Affiliated Hospital of Zhengzhou university ZhengzhouChina The Second Affiliated Hospital of Xi’an Jiaotong university Xi’anChina The Second Affiliated Hospital of Chongqing medical university ChongqingChina Huashan Hospital Fudan UniversityShanghaiChina State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalZhejiang University School of MedicineHangzhouChina 不详

The Chinese Clinical Practice Guidelines for the prevention and treatment ofmother-to-child transmission of hepatitis B virus,developed by the Chinese Society of Infectious Diseases of the Chinese medical Association in 2019,serves as a valuable reference for standardizing the process of preventing mother-to-child transmission in *** new evidence emerges,it is crucial that timely and regular updates are made to the clinical practice guidelines so as to optimize guidance for clinical practice and *** this end,the Infectious Disease Physician Branch of Chinese medical Doctor Association and the Chinese Society of Infectious Diseases of Chinese medical Association,in collaboration with multidisciplinary experts,have updated the guidelines based on the latest domestic and international research advancements and clinical practice,in order to provide guidance and reference for clinicians andmaternal and child healthcare workers.

关键词： guidelines hepatitis B virus mother-to-child transmission prevention treatment update

On the general expressions of finite Hankel transform

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Science China(Physics,Mechanics & Astronomy) 2010年第11期53卷 2125-2130页

作者： JIANG Quan 1,2 & GAO CunFa 1 1 College of Aerospace Engineering,Nanjing university of Aeronautics and Astronautics,Nanjing 210016,China 2 school of Civil Engineering,nantong university,nantong 226019,China 1. College of Aerospace Engineering Nanjing University of Aeronautics and Astronautics Nanjing 210016 China2. School of Civil Engineering Nantong University Nantong 226019 China

The general expressions of finite Hankel transform are naturally deduced with the help of the property of Bessel *** equations in this paper can degenerate into three kinds of boundaries since all the coefficients in the boundary conditions are taken into *** results can be adopted in solving physics problems involving the finite Hankel transform.

关键词： hankel transform bessel function finite integral