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Alternative polyadenylation events in epithelial cells sense endometritis progression in dairy cows

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Journal of Integrative Agriculture 2023年第6期22卷 1820-1832页

作者： meagan J.STOTTS Yangzi ZHANG Shuwen ZHANG Jennifer J.MICHAL Juan VELEZ Bothe HANS Martin MAQUIVAR Zhihua JIANG Department of Animal Sciences and Center for Reproductive Biology Washington State UniversityPullmanWA 99164-7620USA Aurora Organic Farms PlattevilleCO 80651USA

Endometritis(inflammation of the endometrial lining) is one of the most devastating reproductive diseases in dairy cattle, resulting in substantial production loss and causing more than $650 million in lost revenue annually in the *** hypothesize that alternative polyadenylation(APA) sites serve as decisive sensors for endometrium health and disease in dairy cows. Endometrial cells collected from 18 cows with purulent vaginal discharge scored 0 to 2 were used for APA profiling with our whole transcriptome termini site sequencing(WTTS-seq) method. Overall, pathogens trigger hosts to use more differentially expressed APA(DE-APA), more intronic DE-APA, more DE-APA sites per gene and more DE-genes associated with inflammation. Host CD59 molecule(CD59), Fc fragment of IgG receptor IIa(FCGR2A), lymphocyte antigen 75(LY75) and plasminogen(PLG) may serve as initial contacts or combats with pathogens on cell surface, followed by activation of nuclear receptor subfamily 1 group H member 4(NR1H4) to regulate AXL receptor tyrosine kinase(AXL), FGR proto-oncogene, Src family tyrosine kinase(FGR), HCK protooncogene, Src family tyrosine kinase(HCK) and integrin subunit beta 2(ITGB2) for anti-inflammation. This study is the first to show significance of cilium pathways in endometrium health and animal reproduction. MIR21 and MIR30A would be perfect antagonistic biomarkers for diagnosis of either inflammation or anti-inflammation. These novel findings will set precedent for future genomic studies to aid the dairy industry develop new strategies to reduce endometritis incidence and improve fertility.

关键词： organic dairy endometritis alternative polyadenylation infection progression antagonistic biomarkers

Engineering osteoarthritic cartilage model through differentiating senescent human mesenchymal stem cells for testing disease-modifying drugs

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Science China(Life Sciences) 2022年第2期65卷 309-327页

作者： Ning Wang Yuchen He Silvia Liu meagan J.Makarcyzk Guanghua Lei Alexander Chang Peter G Alexander Tingjun Hao Anne-Marie Padget Nuria de Pedro Tsapekos Menelaos Hang Lin Department of Orthopaedic Surgery University of Pittsburgh School of MedicinePittsburgh PA 15219USA Department of Orthopedic Surgery Xiangya HospitalCentral South UniversityChangsha 410008China Department of Pathology University of Pittsburgh School of MedicinePittsburgh PA 15219USA Department of Bioengineering University of Pittsburgh Swanson School of EngineeringPittsburgh PA 15219USA Department of Bioinformatics University of Pittsburgh School of MedicinePittsburgh PA 15219USA McGowan Institute for Regenerative Medicine University of Pittsburgh School of MedicinePittsburgh PA 15219USA Department of Biology University of Pittsburgh Dietrich School of Arts and SciencePittsburgh PA 15219USA Department of Research B2B Life Length SLMadrid 28010Spain Xiangya Third Hospital Central South UniversityChangsha 410013China

Significant cellular senescence has been observed in cartilage harvested from patients with osteoarthritis(OA).In this study,we aim to develop a senescence-relevant OA-like cartilage model for developing disease-modifying OA drugs(DMOADs).Spe-cifically,human bone marrow-derived mesenchymal stromal cells(MSCs)were expanded in vitro up to passage 10(P10-MSCs).Following their senescent phenotype formation,P10-MSCs were subjected to pellet culture in chondrogenic *** from qRT-PCR,histology,and immunostaining indicated that cartilage generated from P10-MSCs displayed both senescent and OA-like phenotypes without using other OA-inducing agents,when compared to that from normal passage 4(P4)-***,the same gene expression differences observed between P4-MSCs and P10-MSC-derived cartilage tissues were also observed between the preserved and damaged OA cartilage regions taken from human samples,as demonstrated by RNA sequencing data and other analysis ***,the utility of this senescence-initiated OA-like cartilage model in drug development was assessed by testing several potential DMOADs and *** results suggest that pre-existing cellular senescence can induce the generation of OA-like changes in *** P4-and P10-MSCs derived cartilage models also represent a novel platform for predicting the efficacy and toxicity of potential DMOADs on both preserved and damaged cartilage in humans.

关键词： osteoarthritis MSC senescence cartilage tissue engineering disease-modifying OA drug senolytic

Impact of distance on postoperative follow-up in patients of pediatric surgery:a retrospective review

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World Journal of Pediatric Surgery 2020年第4期3卷 23-29页

作者： meagan E Wiebe Anna C Shawyer Section of General Surgery Department of SurgeryUniversity of ManitobaWinnipegManitobaCanada Section of Pediatric General Surgery Department of SurgeryChildren’s Hospital Research Institute of ManitobaUniversity of ManitobaWinnipegManitobaCanada

Objective Centralization of medical services in Canada has resulted in patients travelling long distances for healthcare,which may compromise their *** hypothesized that children living farther from a children’s hospital were offered and attended fewer follow-up *** We reviewed children less than 17 years of age referred to the general surgery clinic at a tertiary children’s hospital during a 2-year period who underwent *** statistics were *** We identified 723 *** majority were male(61%)with a median age of 7 years(range 18 days to16 years)and were from the major urban center(MUC)(56.3%).The median distance travelled to hospital for MUC patients was 8.9 km(range 0.9-22 km)vs 119.5 km(range 20.3-1950 km)for non-MUC *** children were offered more follow-up appointments(72.7%vs 60.8%,p<0.05).No significant differences existed in follow-up attendance rates(MUC 88.5%vs non-MUC 89.1%,p=0.84)or postoperative complications(9.8%vs 9.2%,p=0.78).There were no *** Patients living farther from a hospital were offered fewer follow-up appointments,but attended an equivalent rate of follow-ups when offered *** and remote follow-up are underused approaches that can permit follow-up appointments while reducing associated travel time and expenses.

关键词： patients surgery Canada

Gondwana Terrane Assembly in Northern Mozambique:A Snapshot of Crustal Processes in the Monapo Klippe

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地学前缘 2009年第S1期16卷 210-211页

作者： meagan Webster Jodie Miller Christie Rowe Department of Geology Stellenbosch Universityc/o Merriman & Ryneveld Str. Department of Geological Sciences University of Cape Town13 University Avenue

The Nampula Subprovince in Northern Mozambique is a Mesoproterozoic gneiss terrane with a Pan-African(523-550 Ma)amphibolite-facies met- amorphic *** province forms a pivotal part of the evolution and final assembly o... 详细信息

关键词： Monapo Complex Mozambique Gondwana

Incidence of anastomotic stricture after Ivor-Lewis oesophagectomy using a circular stapling device

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World Journal of Gastrointestinal Surgery 2019年第11期11卷 407-413页

作者： Robert Tyler Amit Nair meagan Lau James Hodson Rizwan Mahmood Jan Dmitrewski Department of Upper Gastrointestinal Surgery University Hospitals Birmingham NHS Foundation TrustBirmingham B152THUnited Kingdom Institute of Translational Medicine University Hospitals Birmingham NHS Foundation TrustBirmingham B152THUnited Kingdom Department of Gastroenterology Russells Hall HospitalDudley DY12HQUnited Kingdom

BACKGROUND Benign oesophageal strictures carry a significant level of morbidity,causing burdensome symptoms impacting on quality of ***-oesophagectomy anastomotic stricture rates as high as 41%have been reported in the *** can require endoscopic dilatation,often multiple times to relieve *** aim of the present study was to determine a single surgeons stricture rate in a series of 2-stage Ivor-Lewis procedures,and to identify any independent risk factors in their *** To determine a single surgeons stricture rate in a series of 2-stage Ivor-Lewis procedures,and to identify any independent risk factors in their *** We performed a retrospective analysis of a prospectively collected database of Ivor-Lewis oesophagectomy performed from 2004-2018 to determine the stricture *** database comprised a single-surgeon series of open,two-stage oesophagectomies with a circular stapled intra-thoracic *** location,histology,neoadjuvant chemotherapy,stapler size,T-stage and R-status were analysed to see if they could predict stricture *** was defined as dysphagia requiring endoscopic *** with anastomotic leaks were excluded on the basis they would develop an anastomotic *** One hundred and seventy patients were collected in the *** were excluded on the basis of anastomotic leak,perioperative death and early *** hundred and fifty-four patients(119 males,35 females)with a mean age of 64±10 years were eligible for analysis.A total of 15 patients developed strictures a median of 99 d(interquartile range:84-133)after surgery,giving a Kaplan-Meier estimated stricture rate of 10%at one *** of the factors considered were found to be significantly associated with *** In this study the stricture rate was 10%,with the majority occurring in the first 100 d after *** significant independent factors were found in the deve

关键词： Oesophageal cancer Anastomosis Stricture Stapled Circular

The use of virtual surgical planning and navigation in the treatment of orbital trauma

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Chinese Journal of Traumatology 2017年第1期20卷 9-13页

作者： Alan Scott Herford meagan Miller Floriana Lauritano Gabriele Cervino Fabrizio Signorino Carlo Maiorana Oral and Maxillofacial Surgery 11092 Anderson St. Loma Linda CA 92350 USA Department of Medical Sciences and Odontostomatology University of Messina ME Italy Odontostomatologic Surgery University of Milan Milan 20122 Italy

Virtual surgical planning (VSP) has recently been introduced in craniomaxillofacial surgery with the goal of improving efficiency and precision for complex surgical operations. Among many indications, VSP can also be applied for the treatment of congenital and acquired craniofacial defects, including orbital fractures. VSP permits the surgeon to visualize the complex anatomy of craniofacial region, showing the relationship between bone and neurovascular structures. It can be used to design and print using three-dimensional (3D) printing technology and customized surgical models. Additionally, intraoperative navigation may be useful as an aid in performing the surgery. Navigation is useful for both the surgical dissection as well as to confirm the placement of the implant. Navigation has been found to be especially useful for orbit and sinus surgery. The present paper reports a case describing the use of VSP and computerized navigation for the reconstruction of a large orbital floor defect with a custom implant.

关键词： Neuronavigation Orbital implants Orbital trauma Virtual surgical planning

Expression of Genes Associated with Nickel Resistance in White Spruce (<i>Picea glauca</i>) under Nickel Stress: Analysis of AT2G16800 and <i>NRAMP</i>Genes

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American Journal of Plant Sciences 2020年第8期11卷 1163-1174页

作者： meagan Boyd Kabwe Nkongolo Department of Biology Laurentian University Sudbury Canada Biomolecular Sciences Program Laurentian University Sudbury Canada

Heavy metals such nickel (Ni) can cause toxicity by 1) displacing essential components in the biomolecules, 2) blocking the functional group of molecules, or 3) modifying enzymes, proteins, the plasma membrane, and membrane transporters. The main objective of the present study was to investigate the effect of nickel (Ni) on gene expression of nitrate on gene expression with a focus on the genes coding for the high affinity Ni transporter family protein AT2G16800, and natural resistance-associated macrophage protein (NRAMP). Ni toxicity was assessed by treating seedlings with an aqueous solution of nickel nitrate salt [Ni(NO3)2] at the concentrations of 150 mg, 800 mg, and 1600 mg of nickel per 1 kg of dry soil. RT-qPCR was used to measure the expression of AT2G16800, and NRAMP genes in samples treated with nickel nitrates and controls. The results revealed that P. glauca is resistant to Ni based on lack of plant damage at all nickel concentrations. Ni has no effect on the expression of the AT

Hepatocellular carcinoma surveillance:An evidence-based approach

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World Journal of Gastroenterology 2019年第13期25卷 1550-1559页

作者： Patrick S Harris Ross M Hansen meagan E Gray Omar I Massoud Brendan M McGuire Mohamed G Shoreibah Tinsley Harrison Internal Medicine Residency Program Department of Medicine University of Alabama at Birmingham Division of Gastroenterology and Hepatology Department of Medicine University of Alabama at Birmingham

Hepatocellular carcinoma(HCC) makes up 75%-85% of all primary liver cancers and is the fourth most common cause of cancer related death worldwide. Chronic liver disease is the most significant risk factor for HCC with 80%-90% of new cases occurring in the background of cirrhosis. Studies have shown that early diagnosis of HCC through surveillance programs improve prognosis and availability of curative therapies. All patients with cirrhosis and high-risk hepatitis B patients are at risk for HCC and should undergo surveillance. The recommended surveillance modality is abdominal ultrasound(US) given that it is cost effective and noninvasive with good sensitivity. However, US is limited in obese patients and those with non-alcoholic fatty liver disease(NAFLD). With the current obesity epidemic and rise in the prevalence of NAFLD, abdominal computed tomography or magnetic resonance imaging may be indicated as the primary screening modality in these patients. The addition of alpha-fetoprotein to a surveillance regimen is thought to improve the sensitivity of HCC *** investigation of serum biomarkers is needed. Semiannual screening is the suggested surveillance interval. Surveillance for HCC is underutilized and low adherence disproportionately affects certain demographics such as nonCaucasian race and low socioeconomic status.

关键词： Liver cancer Hepatocellular carcinoma Surveillance

对低碳城市交通的投资可能会带来巨大的经济和社会回报

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对低碳城市交通的投资可能会带来巨大的经济和社会回报