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Antibacterial Activity of Lactic Acid Bacteria and Extraction of Bacteriocin Protein

Advances in Bioscience and Biotechnology 2020年第2期11卷 49-59页

作者： Rabiul Islam md. nur Hossain md. Khasrul alam md. Ekhlas Uddin Mehadi Hasan Rony md. Abu Sayeed Imran md. Firoz alam Divisional DNA Screening Laboratory Faridpur Medical College Hospital Ministry of Women & Children Affairs Faridpur Bangladesh Industrial Microbiology Laboratory Institute of Food Science & Technology Bangladesh Council of Scientific & Industrial research Dhaka Bangladesh Department of Biotechnology & Genetic Engineering Islamic University Kushtia Bangladesh Department of Biochemistry and Molecular Biology Gono Bishwabidyalay Dhaka Bangladesh

Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocin producing Lactobacillus spp. is considered Generally Recognize as Safe (GRAS), useful to control the fast development of pathogens and spoiling microbes in food and feed. Lactobacillus spp. was isolated from traditional winter fermented vegetable cucumber & carrot by the use of selective media. Especially De Man, Rogosa and Sharpe (MRS) Agar media were used to isolate the Lactobacillus species. Morphologically identified by gram staining & colony morphology. Biochemically recognized by catalase, oxidase, MRVP & carbohydrate fermentation test. Antimicrobial activity of Lactobacillus spp. was confirmed by Well Diffusion Method. Molecular characterization of bacteriocin protein and molecular weight determined by SDS PAGE method. The isolate was found to be facultative anaerobic, Gram positive, and catalase negative. The result of antimicrobial activity measured by the Arbitrary Unit (AU/ml) of zone of the inhibition. Six isolates found from the sample but most activities exhibited isolate 4 against Bacillus megaterium (55 mm) zone of diameter. The molecular weight of the washed bacteriocin was calculated to be about 40 kDa (Isolate 1) and 15 kDa & 30 kDa (Isolate 5). Bacteriocin protein reduces chemical preservatives and uses in future as biopreservative in food industry.

关键词： Lactobacillus spp. Bacteriocin Antimicrobial Activity SDS PAGE Biopreservative

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Machine Learning-Enabled Power Scheduling in IoT-Based Smart Cities

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Computers, Materials & Continua 2021年第5期67卷 2449-2462页

作者： Nabeela Awan Salman Khan Mohammad Khalid Imam Rahmani Muhammad Tahir nur alam md.Ryan Alturki Ihsan Ullah Department of Computer Science Government Girls Degree College MardanKPK23200Pakistan Department of Electrical Energy System UETPeshawar25000Pakistan Department of Computer Science College of Computing and InformaticsSaudi Electronic UniversityKSA Department of Smart Computing Kyungdong UniversityGlobal CampusGosung24764South Korea Department of Information Sciences College of Computer and Information SystemsUmm Al-Qura UniversityMakkahSaudi Arabia Robotics Engineering Daegu Gyeongbuk Institute of Science and TechnologySouth Korea

Recent advancements in hardware and communication technologies have enabled worldwide interconnection using the internet of things(IoT).The IoT is the backbone of smart city applications such as smart grids and green energy *** smart cities,the IoT devices are used for linking power,price,energy,and demand information for smart homes and home energy management(HEM)in the smart *** complex smart gridconnected systems,power scheduling and secure dispatch of information are the main research *** challenges can be resolved through various machine learning techniques and data *** this paper,we have proposed a particle swarm optimization based machine learning algorithm known as a collaborative execute-before-after dependency-based requirement,for the smart *** proposed collaborative execute-before-after dependencybased requirement algorithm works in two phases,analysis and assessment of the requirements of end-users and power distribution *** the rst phases,a xed load is adjusted over a period of 24 h,and in the second phase,a randomly produced population load for 90 days is evaluated using particle swarm *** simulation results demonstrate that the proposed algorithm performed better in terms of percentage cost reduction,peak to average ratio,and power variance mean ratio than particle swarm optimization and inclined block rate.

关键词： PSO IBR machine learning IoT smart cities CDBR

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Assessment of Different Traits to Evaluate Genetic Divergence in Some Wheat (<i>Triticum aestivum</i>L.) Genotypes under Late Sowing Condition

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American Journal of Plant Sciences 2021年第5期12卷 734-747页

作者： md. Mukhtar Hossain md. Abul Kalam Azad Touria El-Jaoual Eaton md. nur-alam Siddquie Department of Crop Science and Technology Faculty of Agriculture Rajshahi University Rajshahi Bangladesh Cooperative Extension and Research Lincoln University of Missouri Jefferson City USA Senior Scientific Officer Bangladesh Agricultural Research Institute Shyampur Rajshahi

The research work was undertaken to identify the extent of genetic diversity in different parameters of wheat related to heat tolerance mechanism. Performances of currently available 25 spring wheat genotypes were studied at the Regional Wheat Research Institute, Shyampur, Rajshahi during the winter season of 2016/2017. All these genotypes (25) were grouped into five clusters on the basis of non-hierarchical clustering parameters viz. cluster I (G2, G5, G6, G16, G20), II (G4, G7, G9, G11, G12, G17), III (G10, G24), IV (G1, G13, G19, G21) and V (G3, G8, G14, G15, G18, G22, G23, G25). These groups were arranged into five (5) pairs of clusters viz. cluster I and III;II and III;II and V;III and IV;IV and V considering their similar potentiality of different traits. Results revealed that the maximum number of genotypes (8) was found in cluster V while cluster III comprised minimum genotypes (2). The inter-cluster distance was higher than intra-cluster distances. The highest and second highest eigenvalues belonged to spike/m2 (25.23%) and spikelets/spike (20.18%) respectively, along with positive canonical values in both the vectors 1 and 2 for these two traits identified them as major traits by exposing their highest potential toward genetic divergence. Cluster III produced the highest spike/m2 (388.8), grain/spike (48.1), plant height (89.5 cm), SPAD (54.2), yield (2799 kg/ha) and biomass (7758.1 Kg/ha) with lowest heading days (64.7 days), maturity days (98.7 days), canopy temperature at vegetative stage (21.7°C) and canopy temperature at grain filling stage (22.4°C). In contrast, cl

关键词： Genetic Divergence Heat Tolerance Assessment of Different Traits Cluster Analysis Principal Component Analysis Selection of Genotype

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Analytical Treatment of the Evolutionary (1 + 1)-Dimensional Combined KdV-mKdV Equation via the Novel (G'/G)-Expansion Method

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Journal of Applied Mathematics and Physics 2015年第12期3卷 1571-1579页

作者： md. nur alam Fethi Bin Muhammad Belgacem M. Ali Akbar Department of Mathematics Pabna University of Science & Technology Pabna Bangladesh Department of Mathematics Faculty of Basic Education PAAET Al-Ardhiya Kuwait Department of Applied Mathematics University of Rajshahi Rajshahi Bangladesh

The novel (G'/G)-expansion method is a powerful and simple technique for finding exact traveling wave solutions to nonlinear evolution equations (NLEEs). In this article, we study explicit exact traveling wave solutions for the (1 + 1)-dimensional combined KdV-mKdV equation by using the novel (G'/G)-expansion method. Consequently, various traveling wave solutions patterns including solitary wave solutions, periodic solutions, and kinks are detected and exhibited.

关键词： Novel (G'/G)-Expansion Method (1 + 1)-Dimensional Combined KdV-mKdV Equation Kink Patterns Nonlinear Evolution Equation Solitary Wave Solutions Traveling Wave Solutions

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Organic Amendments Improve Plant Morpho-Physiology and Antioxidant Metabolism in Mitigating Drought Stress in Bread Wheat(Triticum aestivum L.)

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Phyton-International Journal of Experimental Botany 2022年第9期91卷 1959-1972页

作者： Taufika Islam Anee md.nur Nabi Islam Mohamed M.Hassan Abdul Awal Chowdhury Masud md.Mahabub alam Mirza Hasanuzzaman Department of Agronomy Faculty of AgricultureSher-e-Bangla Agricultural UniversityDhaka1207Bangladesh Department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia

Due to the unpredictable climate change,drought stress is being considered as one of the major threats to crop ***(Triticum aestivum *** Gom-26)being a dry season crop frequently faces scarcity of water and results in a lower ***,this experiment aims to explore the role of different organic amendments(OAs)in mitigating drought stress-induced *** pot experiment consisted of different organic amendments ***,vermicompost and poultry manure@0.09 kg m^(−2)soil,biochar@2.5%w/w soil and chitosan@1%w/w soil which was imposed on the plants grown under both well-watered and drought *** showed that drought stress reduced plant height(15%),SPAD value(16%),relative water content(13%),number of spikelet spike^(−1)(17%),number of grains spike^(−1)(12%),and 100-grain weight(18%).Organic amendments act as a protectant and reduce drought stress-induced damages by enhancing the morpho-physiological and yield *** enhanced SPAD value by 18%,number of spikelets spike^(−1)by 20%,number of grains spike^(−1)by 17%,whereas poultry manure increased plant height by 16%under drought condition compared to control *** other OAs applied,vermicompost was proved to be capable of reducing the higher lipid peroxidation and proline content raised by drought *** stress-induced increment of catalase,ascorbate peroxidase and glutathione reductase activities were also efficiently modulated by the organic amendment *** present study concluded that OAs play significant roles in alleviating drought stressinduced damages by improving the morpho-physiological attributes and among the different types of OAs used vermicompost performed better which in addition ceased the production of reactive oxygen species.

关键词： Antioxidant defense biochar cereal crop chitosan compost poultry manure reactive oxygen species vermicompost

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Brachyuran Crab Fauna Character Estimated from Marine Water of Bangladesh and Noted New Record (Crustacea: Decapoda) as Distribution

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Open Journal of Marine Science 2020年第4期10卷 218-232页

作者： nur alam Sanjay Chakraborty md. Muzammel Hossain Mohammad Abdul Baki Shayer Mahmood Ibney alam Chenhong Li Department of Zoology Faculty of Life and Earth Science Jagannath University Dhaka Bangladesh Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution College of Fisheries and Life Science Shanghai Ocean University Shanghai China

As habitat and distribution, in the study a total of 18 crab species were identified, belonging to 10 families (Calappidae, Epialtidae, Gecarcinidae, Grapsidae, Matutidae, Ocypodidae, Panopeidae, Sesarmidae, Varunidae, and Xanthidae) order Decapoda under subphylum crustacean with relevant distinguish morphological and meristic character and geographical position. We investigated crab species of Bangladesh from December 2013 to October 2014. All species samples were collected by hand picking on a baseline survey in Saint Martin’s Island (the only coral island of Bangladesh) and adjacent marine water body of Bay of Bengal, Satkhira area (brackish and freshwater) and mangrove forest area (Sundarban). Among 18 species, 10 crab species from Family Epialtidae (1 species), Gecarcinidae (2 species), Ocypodidae (1 species), Panopeidae (1 species), Sesarmidae (1 species) and Xanthidae (4 species) were newly occurred in the Bay of Bengal, Bangladesh. Cardisoma armatum and Zosymus aeneus species were larger than other enlisted species.

关键词： Crab Species Morphology Distribution Coral Island Bangladesh

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Comparison of Sternal Wound Complication after Off-Pump CABG between Skeletonized and Pedicled LIMA Harvesting: A Single Centre Experience in Bangladesh

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World Journal of Cardiovascular Surgery 2023年第6期13卷 101-110页

作者： M. Asmaul alam Al nur md. Aslam Hossain md. Abir Tazim Chowdhury Farhat Tabassum Nishat Munama Magdum Department of Cardiac Surgery National Institute of Cardiovascular Diseases Dhaka Bangladesh Department of Cardiac Surgery Bangabandhu Sheikh Mujib Medical University Dhaka Bangladesh Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Bangladesh Department of Casualty Dhaka Medical College Hospital Dhaka Bangladesh Department of Community Medicine Shaheed Monsur Ali Medical College Dhaka Bangladesh

Background: Off-pump coronary artery bypass grafting (OPCAB) is a surgical procedure that has gained popularity due to its potential benefits over traditional coronary artery bypass grafting, including reduced morbidity and mortality. However, sternal wound complication (SWC) remains challenging following the procedure. The technique of left internal mammary artery (LIMA) harvesting has been shown to impact the incidence of SWC. This study aimed to compare the incidence of SWC between two techniques of LIMA harvesting, i.e., skeletonized and pedicled. Methods: The study was conducted at the Department of Cardiac Surgery, Bangabandhu Sheikh Mujib Medical University, and included 60 patients who underwent OPCAB. The patients were divided into two groups of 30 each based on the technique of LIMA harvesting used, i.e., skeletonized (group A) or pedicled (group B). The postoperative ICU care was given to each patient as per the protocol. The statistical analysis was conducted using the SPSS version 26.0 for Windows software. Results: The results showed that 5 (8.33%) patients developed SWC, with 1 (1.67%) patient in group A and 4 (6.66%) patients in group B. However, the occurrence of SWC was not statistically significant between the two groups (p = 0.35). The mean age, gender distribution, and comorbidities such as hypertension, diabetes, dyslipidemia, and anemia were also not statistically significant between the two groups. The number of smokers was statistically significant between the two groups (p = 0.03), and the occurrence of SWC was found to be higher in smoker patients in group B (p = 0.04). Preoperative and postoperative parameters such as duration of operation, duration of mechanical ventilation, duration of chest drains, duration of the central venous line, and amount of postoperative mediastinal bleeding were also not statistically significant between the two groups. The distribution of wound complications, duration of ICU stays, and hospital stay between t

关键词： Sternal Wound Complication (SWC) Off-Pump Coronary Artery Bypass Grafting (OPCAB) Left Internal Mammary Artery (LIMA) Skeletonized LIMA Harvesting Pedicle LIMA Harvesting

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Water Soluble Ionic Species in the Atmospheric Fine Particulate Matters (PM2.5) in a Southeast Asian Mega City (Dhaka, Bangladesh)

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Open Journal of Air Pollution 2015年第3期4卷 99-108页

作者： Abdus Salam md. Assaduzzaman Muhammad Nobi Hossain A. K. M. nur alam Siddiki Department of Chemistry Faculty of Science University of Dhaka Dhaka Bangladesh

Atmospheric fine particulate matters (PM2.5) were collected with an Envirotech Instrument (Model APM 550) at the roof of Khundkur Mukarram Hussain Science Building, University of Dhaka, Bangladesh between January and February, 2013. PM2.5 samples were collected on Quartz fiber filters during day and night time. Water soluble ions (sulfate, nitrate, phosphate, chloride, bromide, sodium, potassium and calcium) were analyzed with Ion Chromatography (Model 881, Metrohm Ltd., Switzerland) and Flame photometer (Model PFP7, Jenway, UK). Average PM2.5 mass was 136.1 μg·mǅ during day time and 246.8 μg·mǅ during night time with a total average of 191.4 μg·mǅ. Nighttime PM2.5 concentration was about double compared than that of daytime presumable due to the low ambient temperatures with high emissions from heavy duty vehicles. The 24-hour average PM2.5 mass (average of day and night) was about eight times higher than WHO (25.0 μg·mǅ) and about three times higher than DoE, Bangladesh (65.0 μg·mǅ) limit values. The total average concentrations of sulfate, nitrate, phosphate, bromide, chloride, sodium, potassium and calcium were 5.30, 7.75, 0.62, 0.16, 1.19, 1.30, 8.11, and 3.09 μg·mǅ, respectively. The concentrations of the water soluble ions were much higher during nighttime than daytime except nitrate, bromide and potassium. Excellent correlations were observed between sulfate and nitrate, sodium and chloride, bromide and phosphate indicating joint sources of origin. Potassium, sulfate, nitrate and calcium are the most dominant species in PM2.5. Water soluble ionic components in Dhaka contributed about 15% mass of the PM2.5. Ratio analysis showed that sodium and chloride were from mainly sea salt. Potassium has varieties of sources other than biomass burning. Sulfate and nitrate are mainly from fossil fuel origin. This is the first study of the day and night variation of the water soluble ionic species at the fine particulate mat

关键词： Air Pollution Particulate Matters Water Soluble Ions Sulfate Nitrate

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Synthesis, Spectral Characterization and Thermal Behavior of Newly Derived La(III), Co(III) and Mn(II) Complexes with Schiff Base Derived from Methionine and Salicylaldehyde

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Open Journal of Inorganic Chemistry 2018年第2期8卷 55-69页

作者： Shariful Islam A. K. M. nur alam Siddiki Shahida Begum md. Abdus Salam Department of Chemistry Faculty of Science University of Dhaka Dhaka Bangladesh

Many metal complexes of Schiff base derived from different amino acids are widely employed as biologically active materials, especially as antibacterial agents. Three new metal [Co(III), Mn(II) and La(III)] complexes with the Schiff base (L) derived from salicylaldehyde and amino acid (methionine) were synthesized and investigated by using various physico-chemical techniques such as elemental analysis, FTIR, UV-visible spectroscopy, magnetic measurement, thermo gravimetric analysis (TGA) and X-ray powder diffraction (XRD) method. From spectral studies, it has been concluded that the synthesized ligand acts as a tetra-dentate molecule, coordinates metal through azomethine nitrogen, sulfur, phenolic oxygen and carboxylate oxygen. UV-visible spectrophotometry showed the characteristic absorption bands corresponding to a square planar geometry for La(III) and Mn(II) metal complexes and tetrahedral geometry for Co(III) complex. The XRD data demonstrated that the manganese and cobalt complexes were crystalline but the lanthanum complex was amorphous in nature. The empirical formula of the synthesized complexes based on analytical data were [Co(C12H13SNO3)]·(NO3), [La(C12H13SNO3)](Cl)(H2O) and [Mn (C12H13SNO3)].

关键词： Schiff Base Metal Complexes Spectral Studies Thermal Behavior X-Ray Powder Diffraction

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Quality Assessment of Surface and Drinking Water of Nakla Paurosova, Sherpur, Bangladesh

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Advances in Microbiology 2019年第8期9卷 703-727页

作者： Sourav Sarker Shahin Mahmud Razia Sultana Ritu Biswas Partha Protim Sarkar md. Abul Munayem nur-E-alam md. Rayhan Ali Muhammad Wasim md. Firoz Ali md. Omar Faruque Fatematuz Zuhura Evamoni Department of Biotechnology and Genetic Engineering Mawlana Bhashani Science and Technology University Tangail Bangladesh Department of Biotechnology and Genetic Engineering Noakhali Science and Technology University Noakhali Bangladesh Department of Biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Science and Technology University Gopalganj Bangladesh Department of Biotechnology Bangladesh Agricultural University Mymensingh Bangladesh

Water is one of the most vital elements of ecosystem and human being, but unfortunately nowadays, the pollution of surface and drinking water is an alarming problem. The present work deals with the assessment of physicochemical and bacteriological profile of several pond, jar and tube-well water samples to ensure its suitability for using and drinking. Total 30 samples were randomly selected and collected from Nakla Paurosova of Sherpur district by following the standard procedure. Bacteriological analysis was carried out by following the standard bacteriological methods. Most of the surface water sampling points were polluted by dumping of waste, cattle wash and were not suitable for drinking or other domestic purposes. Among three heavy metals, only Iron was detected in six tube-well water samples, one was also positive to arsenic, rest of the water bodies were negative to all of these metals. In case of most of the water bodies, different physicochemical properties were below standard limit. In pond water, the Total Viable Count (TVC) ranged from 2.7 × 107 cfu/100ml to 4.4 × 1015 cfu/100ml and Total Coliform Count (TCC) were 3.4 × 105 cfu/100ml to 4.8 × 1013 cfu/100ml, where the mean concentration of Heterotrophic Plate Count (HPC) was 2.4 × 105 cfu/100ml and 1.8 × 105 cfu/100ml in jar and tube-well water respectively. On the other hand, the Total Coliform Count of supply water was 33 cfu/100ml and tube-well water was 14 cfu/100ml. Fecal coliform was detected in all of the pond water samples, four jar and three tube-well water too. E. coli was present in all pond water samples, and also detected in 80% supply and 50% tube-well water also. Shigella spp. was found in two pond water and in one supply water, where tube-well water was free from it. Salmonella spp. was also detected in 30% of pond and 20% of supply water, whereas absent in tube-well water. 50% of pond, 40% of supply and 30% of tube-well water were contaminated with Vibrio spp. The total counts of these

关键词： Physiochemical Parameters Bacterial Load Antibiogram Public Health Nakla Paurosova

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