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Physicochemical and Bacteriological Profile of Bilanko and Ngamakala Peat Bog Soils (Republic of Congo)

Journal of Environmental Protection 2024年第8期15卷 842-862页

作者： Gatsé Elgie Viennechie mboukou kimbatsa irène marie cecile Ignoumba Evariste Mesmin Morabandza Cyr Jonas Nguimbi Etienne Laboratory of Microbiology Infectiology and Immunology ENS-UMNG Brazzaville Republic of Congo Laboratory of Cellular and Molecular Biology FST-UMNG Brazzaville Republic of Congo Laboratory of Remote Sensing and Forest Ecology ENS-UMNG Brazzaville Republic of Congo

Peatlands are unique and complex natural ecosystems that are part of the most important carbon reservoirs on our planet, home to a diversity of microorganisms responsible for fermentation, humification or peat. The aim is to understand chemical and biological indicators of peatland soils. This work aims to determine the physicochemical and bacteriological profile and lipolytic activity of soil bacteria in Bilanko peatlands. The bacterial profile with the production of lipases is carried out by classical microbiology techniques. The results show that the soils are moderately acidic with temperatures of 27.8˚C ± 0.01˚C for Bilanko and 27.1˚C ± 0.57˚C for Ngamakala. The electroconductivity (EC) varies from (9.52 ± 0.002) μS/cm to (39.01 ± 1.4) μS/cm with low turbidity of (2.04 ± 0.66) mg/L to (31.02 ± 0.84) mg/L and low ion concentrations with, however, a richness in phenolic compounds for Bilanko compared to Ngamakala. FMAT diversity ranged from (1.71 ± 0.88)∙104 UFC/g to (2.92 ± 0.07)∙105 UFC/g for Bilanko and (1.30 ± 0.73)∙104 UFC/g to (2.89 ± 0.06)∙104 UFC/g for Ngamakala. Bacillus loads ranged from (5.20 ± 1.40)∙103 CFU/g to (1.22 ± 0.13)∙104 CFU/g and from (1.11 ± 0.13)∙104 CFU/g to (9.20 ± 2.05)∙103 CFU/g;enterobacteria loads from (1.40 ± 0.76)∙103 CFU/g to (8.80 ± 1.73)∙103 CFU/g and from (1.01 ± 0.02)∙103 CFU/g to (9.20 ± 2.05)∙103 CFU/g;in Pseudomonas from 0 to (2.30 ± 0.53)∙102 CFU/g and from 0 to (8.90 ± 2.35)∙102 CFU/g for Bilanko and Ngamakala respectively. These results reveal a variation in bacterial similarity and distribution in the Bilanko and Ngamakala peat bogs.

关键词： Bacteria Lipase Polyphenol Soil Peat Bog Republic of Congo

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Rare dentin defects:Understanding the pathophysiological mechanisms of COLXVA1 mutations

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Genes & Diseases 2024年第5期11卷 1-5页

作者： Isaac Maximiliano Bugueno Tristan Rey Alexandra Jimenez-Armijo Marzena Kawczynski Naji Kharouf marie-cecile Maniere Yann Herault Agnes Bloch-Zupan Virginie HaushalterLaugel Institute of Genetics and Molecular and Cellular Biology(IGBMC) CNRS-UMR7104INSERM U1258Universitede StrasbourgIllkirch 67400France Faculty of Dentistry Universitede StrasbourgStrasbourg 67000France Institute of Advanced Studies(USIAS) Universitede StrasbourgStrasbourg 67045France Reference Center for Rare Oral and Dental Diseases(CRMR-O-Rares) Oral Medicine and Surgery DepartmentHopitaux Universitaires de Strasbourg(HUS)Strasbourg 67000France Rare Diseases Health network“TETE COU”&European Reference network ERN CRANIO Strasbourg 67000France “Institut de Genetique Medicale d’Alsace” Laboratory of Genetic DiagnosticHopitaux Universitaires de Strasbourg(HUS)Strasbourg 67000France Biomaterials and Bioengineering Laboratory Inserm UMR_S 1121Strasbourg 67000France Orofacial Development and Regeneration Faculty of MedicineInstitute of Oral BiologyCentre of Dental MedicineUniversity of ZurichZurich 8032Switzerland

Dentin is a mineralized tissue with a chemical composition similar to bone but with a higher mineralized density and *** constitutes the central structure of the tooth between the internal pulp and external enamel toward the oral cavity or cementum toward the underlying *** dentin defects occur in a variety of rare genetic *** can manifest as“isolated”occurrences such as in dentinogenesis imperfecta(DI)or dentin dysplasia(DD)or can be associated with other symptoms in diseases such as osteogenesis imperfecta,Goldblatt syndrome,microcephalic osteodysplastic primordial dwarfism type Ⅱ,among others.

关键词： diseases den rigidity

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Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area, Republic of Congo

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Open Journal of Soil Science 2023年第6期13卷 263-274页

作者： Gatse Elgie Viennechie mboukou kimbatsa irène marie Cécile Morabandza Cyr Jonas Mfoutou Mampanguy Claujeans Chastel Ifo Suspens Averti Nguimbi Etienne Laboratory of Microbiology Infectiology and Immunology ENS Université Marien NGOUABI Brazzaville Republic of Congo Laboratory of Cellular and Molecular Biology Faculty of Sciences and Techniques Université Marien NGOUABI Brazzaville Republic of Congo Molecular Biology and Bioinformatics Unit Faculty of Sciences and Techniques Université Marien Ngouabi Brazzaville Republic of Congo Teledetection and Eorest Ecology Laboratory ENS Université Marien NGOUABI Brazzaville Republic of Congo

This study aimed to the molecular identification of isolated bacteria from the soils of Likouala (Congo-Brazzaville) peat bog area. Counting and isolation were carried out on Mossel and TSB media enriched with petroleum and vegetable oil;the amplification and sequencing of 16S RNA genes by PCR and Artic Oxford Nanopore Technology. The results showed bacterial loads of (5.81 ± 1.08) × 104;(6.64 ± 1.94) × 104 et (8.56 ± 1.19) × 103 CFU/g on Mossel respectively for samples 1, 2 and 3 against (2.12 ± 4.1) × 108 et (8.15 ± 10.1) × 107 CFU/g respectively on TSB enriched with petroleum and vegetable oil exclusively with sample 2. The analysis of the 16S rRNA of the isolates gene made it possible, after PCR, agarose gel electrophoresis, sequencing and bioinformatics analysis, to identify eight (08) strains with similarities of 99 to 100% whose sequences genes placed in GenBank have made it possible to obtain accession numbers corresponding to: Bacillus thuringiensis strain (ON303633);Bacillus cereus strain (ON350770);Bacillus thuringiensis strain (ON350771);Bacillus thuringiensis strain (ON738723);Priestia megaterium strain (ON738719);Bacillus anthracis strain (ON738720);Bacillus subtilis (ON738721);Enterobacter sp (ON738722). The phylogenetic classification of strains was done and revealed two genuses which are Bacillus and Enterobacteriaceae.

关键词： Bacteria Peat Bog Area PCR Sequencing 16S rRNA

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16S rRNA Gene-Based Metagenomic Analysis of Soil Bacterial Diversity in Brazzaville, Republic of the Congo

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Advances in Microbiology 2023年第9期13卷 477-498页

作者： irène marie Cécile mboukou kimbatsa Itsouhou Ngô Armel Ibala Zamba Faly Armel Soloka Mabika Thantique Moutali Lingouangou Joseph Goma-Tchimbakala Etienne Nguimbi Laboratoire de Biologie Cellulaire et Moléculaire (BCM) Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republic of the Congo Unité de Recherche en Bioinformatique et Microbiologie Moléculaire Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republic of the Congo Institut National de Recherche en Sciences Exactes et Naturelles (irSEN) Brazzaville Republic of the Congo

Soil contains a great diversity of microorganisms, among which are bacteria. This study aimed to explore bacterial diversity in soil samples in Brazzaville in the Republic of the Congo. Environmental DNA was extracted. The illumina MiSeq sequencing was held and the diversity indices have been computed. Illumina MiSeq sequencing revealed 21 Phyla, four of which were abundant: Proteobacteria, Acidobacteria, Actinobacteria and Bacteroidetes. Soil microbial communities in the studied samples were phylogenetically diverse but with a stable community structure. 17 classes are represented with relative abundances of Rihzobiales, Bacillales, Actinomycetales and Acidobacteriales. 40 families, the Alphaproteobacteria, the Bacilli and the 12 Actinobacteria. 83 orders among which the Rhizobiales are the most abundant followed by Bacillales and the least abundant followed by the Flavobacteriaceae. Of the 28 genera listed, the Bradyrhizobium is the most dominant in Mw3 and Mw4. 25 listed species, Bradyrhizobium, Bacillus, Actinoplanes, and Candidatu coribacter Acidobacterium are the most abundant species. The Shannon indices of Mw3 and Mw4 are equal, the H’max of Mw4 is greater than the H’max of Mw3. The Simpson index of Mw4 is equal to the Simpson index of Mw3, and the Pielou index (J) of Mw4 is less than the R of Mw3, but very close. This study opens interesting perspectives on the knowledge and exploitation of telluric bacteria in several areas of life.

关键词： Metagenomic Sequencing 16S rRNA Gene Soil Bacteria

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Optimal Low-voltage Distribution Topology with Integration of PV and Storage for Rural Electrification in Developing Countries:A Case Study of Cambodia

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Journal of Modern Power Systems and Clean Energy 2020年第3期8卷 531-539页

作者： Vannak Vai marie-cecile Alvarez-Herault Bertrand Raison Long Bun Department of Electrical and Energy Engineering(GEE) Institute of Technology of Cambodia(ITC)Phnom Penh 12150Cambodia Institute of Engineering UniversitéGrenoble Alpes38000 GrenobleFrance

This paper addresses an optimal design of low-voltage(LV)distribution network for rural electrification considering photovoltaic(PV)and battery energy storage(BES).It aims at searching for an optimal topology of an LV distribution system as well as the siting and sizing of PV and storage over a time horizon of 30 ***,the shortest-path algorithm(SPA)and first-fit bin-packing algorithm(FFBPA)are used to search for the optimal radial topology that minimizes the total length of the distribution line and improves the load ***,the optimal siting of decentralized BES(DeBES)is determined using a genetic algorithm(GA)to eliminate the undervoltage constraints due to the load *** iterative techniques are elaborated to size the maximum peak power of PV and the minimum number of DeBES that can be connected to an LV network without violating the voltage and current ***,the sizing strategy of centralized BES(CeBES)is developed to avoid reverse power flows into the medium-voltage(MV)***,a Monte Carlo approach is used to study the impact of load profile uncertainties on the topology.A non-electrified village in Cambodia has been chosen as a case study.

关键词： Battery energy storage(BES) low-voltage(LV)distribution network Monte Carlo photovoltaic(PV) electrification planning

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Multiyear analyses of ground-level air contaminants over Paris metropolitan region using real-time observations and air mass backward trajectories

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Particuology 2016年第5期14卷 60-71页

作者： marie-cecile G. Chalbot Ioannis Nicolis Chantal Guihenneuc-Jouyaux Ilias G. Kavouras University of Arkansas for Medical Sciences Little Rock AR 72205 USA Universite Paris Descartes 75006 Paris Francec University of Alabama at Birmingham Birmingham AL 35294 USA

For the years 2008-2013, particles of diameter 〈 10 and 2.5 μm （PM10 and PM2.5, respectively）, NOx, SO2, and O3 concentrations at urban, suburban, rural, and traffic sites in the Paris metropolitan area were analyzed. Strong spatial variability at traffic and rural sites and relatively uniform profiles at urban and suburban sites for PM10, PM2.5, and O3 were observed. The O3 weekend effect was induced by lower NOx emissions during the weekend, and favored volatile organic compounds （VOCs）-limited atmospheric conditions. In conJunction with low ambient temperature, these conditions could also favor increased formation of secondary particulate nitrates in winter. Winter air pollution events were associated with multiple pollutants, whereas those observed in spring were caused by high PM10 and PM2.5 levels. Back- ward trajectory analyses showed the contribution of sources in Western and Central Europe on days with high PM10, PM2.5, and O3, and a local/national comvonent for NOx and SO2.

关键词： Air quality Megacity Regional transport PM10 PM2.5 Ozone

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Development of a standardized in vitro approach to evaluate microphysical,chemical,and toxicological properties of combustion-derived fine and ultrafine particles

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Journal of Environmental Sciences 2022年第3期34卷 104-117页

作者： Ana Teresa Juarez-Facio Clement Castilla cecile Corbiere Helene Lavanant Carlos Afonso Christophe Morin Nadine Merlet-Machour Laurence Chevalier Jean-marie Vaugeois Jerome Yon Christelle Monteil Normandie Univ UNIROUENUNICAEN ABTE76000 RouenFrance Normandie Univ UNIROUENINSA RouenCNRSCOBRA76000 RouenFrance Normandie Univ UNIROUENINSA RouenCNRSGPM-UMR663476000 RouenFrance Normandie Univ UNIROUENINSA RouenCNRSCORIA76000 RouenFrance

Ultrafine particles represent a growing concern in the public health community but their precise role in many illnesses is still unknown. This lack of knowledge is related to the experimental difficulty in linking their biological effects to their multiple properties, which are important determinants of toxicity. Our aim is to propose an interdisciplinary approach to study fine(FP) and ultrafine(UFP) particles, generated in a controlled manner using a mini CAST(Combustion Aerosol Standard) soot generator used with two different operating conditions(CAST1 and CAST3). The chemical characterization was performed by an untargeted analysis using ultra-high resolution mass spectrometry. In conjunction with this approach, subsequent analysis by gas chromatography–mass spectrometry(GC–MS) was performed to identify polycyclic aromatic hydrocarbons(PAH). CAST1 enabled the generation of FP with a predominance of small PAH molecules, and CAST3 enabled the generation of UFP, which presented higher numbers of carbon atoms corresponding to larger PAH molecules. Healthy normal human bronchial epithelial(NHBE) cells differentiated at the air-liquid interface(ALI) were directly exposed to these freshly emitted FP and UFP. Expression of MUC5AC, FOXJ1, OCLN and ZOI as well as microscopic observation confirmed the ciliated pseudostratified epithelial phenotype. Study of the mass deposition efficiency revealed a difference between the two operating conditions, probably due to the morphological differences between the two categories of particles. We demonstrated that only NHBE cells exposed to CAST3 particles induced upregulation in the gene expression of IL-8 and NQO1. This approach offers new perspectives to study FP and UFP with stable and controlled properties.

关键词： In vitro Air-liquid interface MiniCAST Fine and ultrafine particles Organic compounds OC/TC

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When parasitoids deal with the spatial distribution of their hosts: consequences for both partners

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Insect Science 2019年第5期26卷 923-931页

作者： Philippe Louapre cecile Le Lann Thierry Hance Earth and Life Institue Universite Catholique de LouvainLouvain-la-NeuveBelgium CNRS Biogeosciences UMR 6282Universite Bourgogne Franche-ComteDijonFrance UMR CNRS 6553 Ecobio University of Rennes 1Rennes CedexFrance

Insect parasitoids developing inside hosts face a true challenge: hosts are scattered in the field and their localization and selection require the use of complex and sometime confusing information. It was assumed for a long time that small-brained organisms like parasitoids have evolved simple and efficient behavioral mechanisms, leading them to be adapted to a given ecological situation, for example, the spatial distribution o f hosts in the habitat. However, hosts are not static and their distribution may also vary through generations and within the life of parasitoid individuals. We investigated if and how parasitoids deal with such a spatial com plexity in a m esocosm experiment. We used the Aphidius rhopalosiphi/Sitobion avenae parasitoid/host system to investigate if parasitoid females experiencing different host aggregation levels exhibit different foraging behaviors independently of the number of hosts in the environment. We showed that A. rhopalosiphi females exploited hosts more intensively both within and among patches at higher host aggregation levels. We discussed the adaptiveness of such behaviors in the light of evolution and biological control.

关键词： Aphidius rhopalosiphi foraging behavior parasitoid sequential adjustment Sitobion avenae spatial distribution

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Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area,Republic of Congo

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Open Journal of Soil Science（土壤科学期刊） 2023年第6期

作者： Gatse Elgie Viennechie mboukou kimbatsa irène marie Cécile Morabandza Cyr Jonas Mfoutou Mampanguy Claujeans Chastel Ifo Suspens Averti Nguimbi Etienne

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16S rRNA Gene-Based Metagenomic Analysis of Soil Bacterial Diversity in Brazzaville,Republic of the Congo

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Advances in Microbiology（微生物学） 2023年第9期

作者： irène marie Cécile mboukou kimbatsa Itsouhou Ngô Armel Ibala Zamba Faly Armel Soloka Mabika Thantique Moutali Lingouangou Joseph Goma-Tchimbakala Etienne Nguimbi

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