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Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area, Republic of congo

Open Journal of Soil Science 2023年第6期13卷 263-274页

作者： Gatse Elgie Viennechie mboukou kimbatsa irène marie cécile Morabandza cyr Jonas Mfoutou Mampanguy claujeans chastel Ifo Suspens Averti Nguimbi Etienne Laboratory of Microbiology Infectiology and Immunology ENS Université Marien NGOUABI Brazzaville Republic of Congo Laboratory of cellular and Molecular Biology Faculty of Sciences and Techniques Université Marien NGOUABI Brazzaville Republic of Congo Molecular Biology and Bioinformatics Unit Faculty of Sciences and Techniques Université Marien Ngouabi Brazzaville Republic of Congo Teledetection and Eorest Ecology Laboratory ENS Université Marien NGOUABI Brazzaville Republic of Congo

This study aimed to the molecular identification of isolated bacteria from the soils of Likouala (congo-Brazzaville) peat bog area. counting and isolation were carried out on Mossel and TSB media enriched with petroleum and vegetable oil;the amplification and sequencing of 16S RNA genes by PcR and Artic Oxford Nanopore Technology. The results showed bacterial loads of (5.81 ± 1.08) × 104;(6.64 ± 1.94) × 104 et (8.56 ± 1.19) × 103 cFU/g on Mossel respectively for samples 1, 2 and 3 against (2.12 ± 4.1) × 108 et (8.15 ± 10.1) × 107 cFU/g respectively on TSB enriched with petroleum and vegetable oil exclusively with sample 2. The analysis of the 16S rRNA of the isolates gene made it possible, after PcR, agarose gel electrophoresis, sequencing and bioinformatics analysis, to identify eight (08) strains with similarities of 99 to 100% whose sequences genes placed in GenBank have made it possible to obtain accession numbers corresponding to: Bacillus thuringiensis strain (ON303633);Bacillus cereus strain (ON350770);Bacillus thuringiensis strain (ON350771);Bacillus thuringiensis strain (ON738723);Priestia megaterium strain (ON738719);Bacillus anthracis strain (ON738720);Bacillus subtilis (ON738721);Enterobacter sp (ON738722). The phylogenetic classification of strains was done and revealed two genuses which are Bacillus and Enterobacteriaceae.

关键词： Bacteria Peat Bog Area PcR Sequencing 16S rRNA

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Physicochemical and Bacteriological Profile of Bilanko and Ngamakala Peat Bog Soils (Republic of congo)

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Journal of Environmental Protection 2024年第8期15卷 842-862页

作者： Gatsé Elgie Viennechie mboukou kimbatsa irène marie cecile Ignoumba Evariste Mesmin Morabandza cyr Jonas Nguimbi Etienne Laboratory of Microbiology Infectiology and Immunology ENS-UMNG Brazzaville Republic of Congo Laboratory of cellular and Molecular Biology FST-UMNG Brazzaville Republic of Congo Laboratory of Remote Sensing and Forest Ecology ENS-UMNG Brazzaville Republic of Congo

Peatlands are unique and complex natural ecosystems that are part of the most important carbon reservoirs on our planet, home to a diversity of microorganisms responsible for fermentation, humification or peat. The aim is to understand chemical and biological indicators of peatland soils. This work aims to determine the physicochemical and bacteriological profile and lipolytic activity of soil bacteria in Bilanko peatlands. The bacterial profile with the production of lipases is carried out by classical microbiology techniques. The results show that the soils are moderately acidic with temperatures of 27.8˚c ± 0.01˚c for Bilanko and 27.1˚c ± 0.57˚c for Ngamakala. The electroconductivity (Ec) varies from (9.52 ± 0.002) μS/cm to (39.01 ± 1.4) μS/cm with low turbidity of (2.04 ± 0.66) mg/L to (31.02 ± 0.84) mg/L and low ion concentrations with, however, a richness in phenolic compounds for Bilanko compared to Ngamakala. FMAT diversity ranged from (1.71 ± 0.88)∙104 UFc/g to (2.92 ± 0.07)∙105 UFc/g for Bilanko and (1.30 ± 0.73)∙104 UFc/g to (2.89 ± 0.06)∙104 UFc/g for Ngamakala. Bacillus loads ranged from (5.20 ± 1.40)∙103 cFU/g to (1.22 ± 0.13)∙104 cFU/g and from (1.11 ± 0.13)∙104 cFU/g to (9.20 ± 2.05)∙103 cFU/g;enterobacteria loads from (1.40 ± 0.76)∙103 cFU/g to (8.80 ± 1.73)∙103 cFU/g and from (1.01 ± 0.02)∙103 cFU/g to (9.20 ± 2.05)∙103 cFU/g;in Pseudomonas from 0 to (2.30 ± 0.53)∙102 cFU/g and from 0 to (8.90 ± 2.35)∙102 cFU/g for Bilanko and Ngamakala respectively. These results reveal a variation in bacterial similarity and distribution in the Bilanko and Ngamakala peat bogs.

关键词： Bacteria Lipase Polyphenol Soil Peat Bog Republic of congo

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Molecular Identification of Isolated Bacteria from Soils in Likouala Peat Bog Area,Republic of congo

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Open Journal of Soil Science（土壤科学期刊） 2023年第6期

作者： Gatse Elgie Viennechie mboukou kimbatsa irène marie cécile Morabandza cyr Jonas Mfoutou Mampanguy claujeans chastel Ifo Suspens Averti Nguimbi Etienne

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16S rRNA Gene-Based Metagenomic Analysis of Soil Bacterial Diversity in Brazzaville, Republic of the congo

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Advances in Microbiology 2023年第9期13卷 477-498页

作者： irène marie cécile mboukou kimbatsa Itsouhou Ngô Armel Ibala Zamba Faly Armel Soloka Mabika Thantique Moutali Lingouangou Joseph Goma-Tchimbakala Etienne Nguimbi Laboratoire de Biologie cellulaire et Moléculaire (BcM) Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republic of the Congo Unité de Recherche en Bioinformatique et Microbiologie Moléculaire Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Republic of the Congo Institut National de Recherche en Sciences Exactes et Naturelles (irSEN) Brazzaville Republic of the Congo

Soil contains a great diversity of microorganisms, among which are bacteria. This study aimed to explore bacterial diversity in soil samples in Brazzaville in the Republic of the congo. Environmental DNA was extracted. The illumina MiSeq sequencing was held and the diversity indices have been computed. Illumina MiSeq sequencing revealed 21 Phyla, four of which were abundant: Proteobacteria, Acidobacteria, Actinobacteria and Bacteroidetes. Soil microbial communities in the studied samples were phylogenetically diverse but with a stable community structure. 17 classes are represented with relative abundances of Rihzobiales, Bacillales, Actinomycetales and Acidobacteriales. 40 families, the Alphaproteobacteria, the Bacilli and the 12 Actinobacteria. 83 orders among which the Rhizobiales are the most abundant followed by Bacillales and the least abundant followed by the Flavobacteriaceae. Of the 28 genera listed, the Bradyrhizobium is the most dominant in Mw3 and Mw4. 25 listed species, Bradyrhizobium, Bacillus, Actinoplanes, and candidatu coribacter Acidobacterium are the most abundant species. The Shannon indices of Mw3 and Mw4 are equal, the H’max of Mw4 is greater than the H’max of Mw3. The Simpson index of Mw4 is equal to the Simpson index of Mw3, and the Pielou index (J) of Mw4 is less than the R of Mw3, but very close. This study opens interesting perspectives on the knowledge and exploitation of telluric bacteria in several areas of life.

关键词： Metagenomic Sequencing 16S rRNA Gene Soil Bacteria

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16S rRNA Gene-Based Metagenomic Analysis of Soil Bacterial Diversity in Brazzaville,Republic of the congo

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Advances in Microbiology（微生物学） 2023年第9期

作者： irène marie cécile mboukou kimbatsa Itsouhou Ngô Armel Ibala Zamba Faly Armel Soloka Mabika Thantique Moutali Lingouangou Joseph Goma-Tchimbakala Etienne Nguimbi

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