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A One-Health Sampling Strategy to Explore the Dissemination and Relationship Between Colistin Resistance in Human,Animal,and Environmental Sectors in Laos

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Engineering 2022年第8期8卷 45-56页

作者： Yuqing Zhou Refath Farzana Somsavanh Sihalath Sayaphet Rattanavong Manivanh Vongsouvath mayfong mayxay Kirsty Sands Paul N.Newton David A.B.Dance Brekhna Hassan Timothy R.Walsh Department of Zoology University of OxfordOxford OX13SZUK Ineos Oxford Institute for Antimicrobial Research(IOI) University of OxfordOxford OX13REUK Department of Medical Microbiology Institute of Infection and ImmunitySchool of MedicineCardiff UniversityCardiff CF144XNUK Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit Microbiology LaboratoryMahosot HospitalVientiane PO Box 292Lao PDR Centre for Tropical Medicine&Global Health Nuffield Department of MedicineUniversity of OxfordOxford OX37BNUK Institute of Research and Education Development University of Health SciencesVientiane PO Box 7444Lao PDR Faculty of Infectious and Tropical Diseases London School of Hygiene and Tropical MedicineLondon WC1E 7HTUK

This study was designed to investigate the molecular epidemiology of mobile colistin resistance(mcr)using a"One-Health"approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination of *** collected 673 samples from humans(rectal normal flora),poultry,and the environment(water,flies,birds,etc.)in Vientiane,Lao People’s Democratic Republic(Laos),from May to September 2018.A total of 238 Escherichia coli(***)isolated from nonduplicative samples,consisting of 98 MCR-positive ***(MCRPEC)("mcr"denotes the gene encoding mobile colistin resistance,and"MCR"denotes the subsequent protein encoded by mcr)and 140 MCRnegative ***(MCRNEC),were characterized by phenotype and Illumina sequencing.A subset of MCRPEC was selected for Min ION sequencing,conjugation assay,plasmid stability,and growth kinetics in *** prevalence of MCRPEC was found to be 14.6%(98/673),with the highest prevalence in human rectal swabs(45.9%(45/98),p<0.0001,odds ratio(OR):0.125,95% confidence interval(CI):0.077-0.202).The percentages of MCRPEC from other samples were 14.3%(2/14)in dog feces,12.0%(24/200)in flies,11.0%(11/100)in chicken meat,8.9%(8/90)in chicken cloacal,8.0%(4/50)in chicken caeca,and 7.5%(4/53)in *** was significantly more resistant to co-amoxiclav,sulfamethoxazoletrimethoprim,levofloxacin,ciprofloxacin,and gentamicin than MCRNEC(p<0.05).Genomic analysis revealed the distribution of MCRPEC among diverse clonal *** putative plasmid Inc types associated with mcr-1 were Inc X4,Inc HI2,Inc P1,Inc I2,and Inc FIA,and those associated with mcr-3 were Inc FII,Inc FIA,Inc FIB,Inc P1,and Inc *** of highly similar plasmids from both flies and other sampling sectors implied the role of flies in the dissemination of ***-positive plasmids were shown to be conjugative,and a significantly high transfer rate into a hypervirulent clone ST1193 was *** containing mcr irrespective of Inc type w

关键词： Colistin resistance mcr Escherichia coli Laos Horizontal transmission

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