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Screening for Panton-Valentine Leukocidin Toxin Genes in Multi-Drug Resistant Methicillin-Resistant <i>Staphylococcus aureus</i>Nasal Isolates from Healthy School Children in Mariental, Namibia

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Journal of Biosciences and Medicines 2020年第12期8卷 13-19页

作者： Sunette Walter Ronnie Böck Biological Sciences Department University of Namibia Windhoek Namibia

Panton-Valentine leukocidin (PVL) is one of the toxins responsible for increased virulence of Staphylococcus aureus. In school settings where children are in close contact with each other, S. aureus strains, including those that may produce PVL, can be transmitted and spread in the community. Twenty-two multi-drug resistant MRSA nasal isolates from children enrolled in five schools in the town of Mariental and the multi-drug resistant American Type Culture Collection MRSA reference strain S. aureus ATCC 33591 (PVL-negative control) were used for molecular assays. Plasmid deoxyribonucleic acid (DNA) of isolates was amplified by polymerase chain reaction (PCR) and amplified PCR products were electrophoresed on a 2.5% (w/v) agarose gel containing 12 μl 0.5 μg/ml ethidium bromide and 1× TAE (Tris-acetate-EDTA) buffer at 90 volts for 50 minutes. The developed gel was viewed for the PVL-associated lukS and lukF genes that amplified at 151 bp and 406 bp, respectively. Our results indicated that seven nasal isolates had PVL toxin gene(s). From the seven isolates, three were tested positive for both lukS and lukFgenes, one tested positive for only lukS, and three tested positive for only lukF. Two of the isolates harbouring both lukS and lukF genes shared the same antibiotic resistance pattern and one of them could also produce enterotoxin A. One of the isolates with only lukF gene could produce enterotoxins B and C. These toxin-producing isolates can be expected to be more virulent than non-producers. Children should be educated on the importance of regular handwashing with soap and water to prevent the spread of potentially virulent staphylococci amongst them and the wider community. This work warrants a larger study to be carried out to investigate PVL toxin and its associated infections in Staphylococcus from school children in Namibia.

关键词： Methicillin-Resistant Staphylococcus aureus Panton-Valentine Leukocidin Children

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谁动了情

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作者： ck

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三轴形变核^(131)Ba中的奇异集体激发模式

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物理学报 2024年第11期73卷 89-100页

作者：韦锐周厚兵王思成丁兵强赟华贾晨旭陈红星郭松 C.M.Petrache D.Mengoni A.Astier E.Dupont 吕冰锋 D.Bazzacco A.Boso A.Goasduff F.Recchia D.Testov F.Galtarossa G.Jaworski D.R.Napoli S.Riccetto M.Siciliano J.J.Valiente-Dobon C.Andreoiu F.H.Garcia K.Ortner K.Whitmore A.Ataç-Nyberg T.Bäck B.Cederwall E.A.Lawrie I.Kuti D.Sohler T.Marchlewski J.Srebrny A.Tucholski 广西师范大学物理科学与技术学院桂林541004 广西核物理与核技术重点实验室桂林541004 中国科学院近代物理研究所兰州730000 中国科学院大学北京100049 巴黎萨克雷大学 CNRS/IN2P3IJCLab奥赛91405 帕多瓦大学物理和天文学系帕多瓦I-35131 意大利国家核物理研究所帕多瓦分部帕多瓦I-35131 意大利国家核物理研究所莱尼亚罗国家实验室莱尼亚罗(帕多瓦)I-35020 费拉拉大学理学与自然科学院费拉拉西蒙菲莎大学化学系本拿比BC V5A 1S6 皇家理工学院物理系斯德哥摩尔S-10691 国家研究基金会iThemba加速器科学实验室西萨默塞特7123 西开普大学物理与天文系贝尔维尔ZA-7535 HUN-REN原子核研究所德布勒森4001 华沙巴斯德拉大学重离子实验室华沙02-093

利用意大利Legnaro实验室串列静电加速器提供的65 MeV^(13)C束流与122Sn靶的熔合蒸发反应布居了^(131)Ba的高自旋态,并搭建了新的能级纲图.新的能级纲图由15条转动带组成,包括三对手征双重带,其中两对正宇称手征带为赝自旋-手征四重带.... 详细信息

利用意大利Legnaro实验室串列静电加速器提供的65 MeV^(13)C束流与122Sn靶的熔合蒸发反应布居了^(131)Ba的高自旋态,并搭建了新的能级纲图.新的能级纲图由15条转动带组成,包括三对手征双重带,其中两对正宇称手征带为赝自旋-手征四重带.正负宇称手征带通过一系列增强的E1跃迁连接,表明它们之间存在八极关联.在低自旋区域搭建了一条新的转动带,通过一系列γ跃迁向νh1_(1/2)晕带退激.这种能级结构与摇摆带相似,但基于现有的实验证据还难以将它确认为摇摆带,不能排除其他集体激发机制,如γ振动的影响.

关键词：高自旋态手征带赝自旋八极关联摇摆运动

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Enhanced reversibility of the magnetoelastic transition in(Mn,Fe)2(P,Si)alloys via minimizing the transition-induced elastic strain energy

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Journal of Materials Science & Technology 2022年第8期103卷 165-176页

作者： Xuefei Miao Yong Gong Fengqi Zhang Yurong You Luana Caron Fengjiao Qian Wenhui Guo Yujing Zhang Yuanyuan Gong Feng Xu Niels van Dijk Ekkes Brück MIIT Key Laboratory of Advanced Metallic and Intermetallic Materials Technology School of Materials Science and EngineeringNanjing University of Science and TechnologyNanjing 210094China Fundamental Aspects of Materials and Energy Department of Radiation Science and TechnologyDelft University of TechnologyMekelweg 15DelftJB 2629Netherlands Department of Physics Bielefeld UniversityBielefeld 33501Germany Helmholtz-Zentrum Berlin für Materialien und Energie Berlin 12489Germany College of Physics Nanjing University of Aeronautics and AstronauticsNanjing 210016China

Magnetocaloric materials undergoing reversible phase transitions are highly desirable for magnetic refrigeration applications.(Mn,Fe)_(2)(P,Si)alloys exhibit a giant magnetocaloric effect accompanied by a magnetoelastic transition,while the noticeable irreversibility causes drastic degradation of the magnetocaloric properties during consecutive cooling *** the present work,we performed a comprehensive study on the magnetoelastic transition of the(Mn,Fe)_(2)(P,Si)alloys by high-resolution transmission electron microscopy,in situ field-and temperature-dependent neutron powder diffraction as well as density functional theory calculations(DFT).We found a generalized relationship between the thermal hysteresis and the transition-induced elastic strain energy for the(Mn,Fe)_(2)(P,Si)*** thermal hysteresis was greatly reduced from 11 to 1 K by a mere 4 at.%substitution of Fe by Mo in the Mn_(1.15)Fe_(0.80)P_(0.45)Si_(0.55)*** reduction is found to be due to a strong reduction in the transition-induced elastic strain *** significantly enhanced reversibility of the magnetoelastic transition leads to a remarkable improvement of the reversible magnetocaloric properties,compared to the parent *** on the DFT calculations and the neutron diffraction experiments,we also elucidated the underlying mechanism of the tunable transition temperature for the(Mn,Fe)_(2)(P,Si)family,which can essentially be attributed to the strong competition between the covalent bonding and the ferromagnetic exchange *** present work provides not only a new strategy to improve the reversibility of a first-order magnetic transition but also essential insight into the electron-spin-lattice coupling in giant magnetocaloric materials.

关键词： Magnetocaloric effect (Mn Fe)2(P Si) Hysteresis Neutron diffraction

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LARGE-SCALE SOLAR HEATING AND COOLING SYSTEMS IN EUROPE

LARGE-SCALE SOLAR HEATING AND COOLING SYSTEMS IN EUROPE

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2007世界太阳能大会：太阳能与人类居住

作者： Jan-Olof Dalenbck Chalmers University of Technology Dept.of Energy and Environment SE-412 96 Gteborg Sweden

In total,there are about 19 million m of glazed solar collectors in Europe,corresponding to about 13,5 GW, where the majority of the collectors are installed in small systems with up to a few ***,the relevant heat loads vary from a few kWth up to several GWand about 9% of the heating loads in Europe are covered by block and district heating *** far,only a minor part of the European collector market comprises large-scale *** large-scale solar heating plants having more than 500 m(～350 kW)of solar collectors have only about 140 MWaltogether,*** 1% of the present installations in *** is thus a need to develop large-scale applications in order to utilize the full potential of solar heat to cover existing heat loads. The first large-scale solar heating applications were introduced in Sweden in the late 70’*** in large-scale solar heating,especially in Germany and Austria,increased during the 90’s and about 100 plants with more than 500 m of solar collectors are put into operation since mid 90’***-scale plants show more favourable costs of solar heat than most small systems and a strong market growth for small systems in Spain and France could now lead to an increased interest for large-scale applications also in Southern Europe. However,Sweden is still the leading country with 22 out of about 120 European large-scale plants(>500 m;>350 kW)in operation. The majority of large-scale solar applications are installed in block and district heating systems,but there are now also an increasing number of solar cooling *** paper gives an update on the present status and describes initial visions and strategies to increase the development of large-scale solar thermal applications within the European Solar Thermal Technology Platform(ESTTP)in co-operation with representatives for Euroheat & Power.

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Enigmatic Glass-Like Carbon from the Alpine Foreland, Southeast Germany: A Natural Carbonization Process

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Acta Geologica Sinica(English Edition) 2018年第6期92卷 2179-2200页

作者： Tatyana G. SHUMILOVA Sergey I. ISAENKO Vasily V. ULYASHEV Boris A. MAKEEV Michael A. RAPPENGLück Aleksey A. VELIGZHANIN Kord ERNSTSON Institute of Geology of Komi Scientific Center of Ural Branch of Russian Academy of Sciences Pervomayskaya st.54Syktyvkar167982Russia Hawaii Institute of Geophysics and Planetology University of Hawaii at Manoa1680 East-West RoadHonoluluHI96822USA Institute for Interdisciplinary Studies GilchingGermany National Research Center 《Kurchatov Institute》 Akademika Kurchatova pl.1Moscow123182Russia Faculty of Philosophy I University of WürzburgGermany

Unusual carbonaceous matter, termed here chiemite, composed of more than 90% C from the Alpine Foreland at Lake Chiemsee in Bavaria, southeastern Germany has been investigated using optical and atomic force microscopy, X-ray fluorescence spectroscopy, scanning and transmission electron microscopy, high-resolution Raman spectroscopy, X-ray diffraction and differential thermal analysis, as well as by δ13 C and 14 C radiocarbon isotopic data analysis. In the pumice-like fragments, poorly ordered carbon matter co-exists with high-ordering monocrystalline α-carbyne, and contains submicrometersized inclusions of complex composition. Diamond and carbyne add to the peculiar mix of matter. The required very high temperatures and pressures for carbyne formation point to a shock event probably from the recently proposed Holocene Chiemgau meteorite impact. The carbon material is suggested to have largely formed from heavily shocked coal, vegetation like wood, and peat from the impact target area. The carbonization/coalification high PT process may be attributed to a strong shock that instantaneously caused the complete evaporation and loss of volatile matter and water, which nevertheless preserved the original cellular structure seen fossilized in many fragments. Relatively fresh wood encapsulated in the purported strongly shocked matter point to quenched carbon melt components possibly important for the discussion of survival of organic matter in meteorite impacts, implying an astrobiological relationship.

关键词： astrobiology diamond carbyne coalification carbonization meteorite impact chiemite

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新爱美运动

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2005年

作者： ck国际传播

独自在家运动，是了解自己身材曲线、身体部位的最佳时机，你可以高兴地欣赏它的完美，也不用担心被别人看见它的缺点，并在每日努力运动、汗水滴落的当下，愉悦地发现自己达到心中完美的曲线目标！全书集结四种易学有效的运动，在NIKE... 详细信息

ISBN: (纸本)7506276046

独自在家运动，是了解自己身材曲线、身体部位的最佳时机，你可以高兴地欣赏它的完美，也不用担心被别人看见它的缺点，并在每日努力运动、汗水滴落的当下，愉悦地发现自己达到心中完美的曲线目标！全书集结四种易学有效的运动，在NIKE体适能专家VIckY的专业指导，时下女……

关键词：文化、科学、教育、体育体育其他体育运动

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42GHz Active Frequency Doubler in SiGe Bipolar Technology

42GHz Active Frequency Doubler in SiGe Bipolar Technology

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2002 3rd International Conference on Microwave and Millimeter Wave Technology

作者： Sabine Hackl Josef Bck Infineon Technologies AG Otto-Hahn-Ring 6D-81730 MunichGermany Institute of Communications and Radio-Frequency Engineering Vienna University of TechnologyGusshausstrasse 251040 ViennaAustria

＜正＞This work describes a monolithic integrated active frequency doubler for frequencies up to 42 GHz in a pre-production 0.4μm SiGe bipolar *** is achieved at the input power of -12dBm between 18 and 42 GHz with maximum of 8.6 dB at 30 *** circuit may be used for future broadband wireless services like LMDS at 28 and 38 GHz or MVDS at 42 GHz.

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Detection of microbial antigenic components of circulating immune complexes in HIV patients:Involvement in CD4^+ T lymphocyte count depletion

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Asian Pacific Journal of Tropical Medicine 2010年第10期3卷 828-832页

作者： Ezeani Michael Chukwudi Onyenekwe CC Wachukwu ck Anyiam DCD Meludu SC Ukibe RN Ifeanyichukwu M Onochie A Anahalu I Okafor UU Department of Immunology Nnamdi Azikiwe University Nnewi CampusAnambra State Nigeria Department of Medical Laboratory Science Nnamdi Azikiwe University Nnewi CampusAnambra State Nigeria Department of Medical Laboratory Science Rivers State University of Science and TechnologyPort-HarcourtRiver State Nigeria Department of Histopathology Nnamdi Azikiwe University Nnewi CampusAnambra State Nigeria Department of Human Biochemistry Nnamdi Azikiwe University Nnewi CampusAnambra State Nigeria Department of Biochemistry Anambra State UniversityUli Anambra State Nigeria Department of Medical Laboratory Science Microbiology UnitNnamdi Azikiwe University Teaching HospitalNnewiAnambra State Nigeria Department of Pharmacology Nnamdi Azikiwe University Nnewi CampusAnambra State Nigeria

Objective:To investigate the prevalence of microbial antigenic components of circulating immune complexes amongst grades of CD4 T lymphocyte counts in HIV sero positive and seronegative ***:Polyethelene glycol(PEG-600) and buffering methods of precipitation and dissociation of immune complexes was used to generate immune solution from sera of 100 HIV sero-positive and 100 HIV sero-negative *** were categorized into 3 grades based on CD4 count:> 500 cell/mm,200-499 cell/mm3 and <200 cell/*** immune solutions were assayed using membrane based immunoassay and antibody titration, along side its unprocessed serum for detection of various microbial antigens and or antibodies. CD4 T cell counts were estimated using Patec Cyflow SL-3 ***:Antigenic component of immune complexes of various infectious agents was detected in 99 and 70 HIV seropositive and HIV sero-negative participants,*** group A,there were 10 HIV positive participants,including 4(40.0%) had circulating immune complexes(CICs) due to Salmonella species only:1(10.0%) due to Salmonella-Plasmodium falciparum(***),SalmonellaP. falciparum-HCV and *** antigens,*** group B,45(45.4%) HIV seropositive participants with CICs had CD4 T lymphocyte count between 200-499 cells/mm^*** of these,20(44.4%) had CICs due to Salmonella species only:9(20%) due to Salmonella-P. *** group C,there were 44(44.4%) HIV sero-positive participants,including 3(6.8%) due to Salmonella species only:24(54.4%) due to ***:2(4.5%) due to P. falciparum ***:In HIV sero-positive participants,presence of heterogeneity of Salmonella *** antigens was highly incriminated in CD4 count depletion but not homogeneity of malaria parasites *** parasites antigens only were incriminated in CD4^+ count depletion amongst HIV sero-negative *** taking any decision on the management

关键词： HIV/AIDS Immune complexes Microbial antigens HIV positive participant CD4^+ lymphocyte count

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90年代血友病:来自世界卫生组织和世界血友病联盟联合会议的报告

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国外医学（输血及血液学分册） 1992年第4期15卷 222-223页

作者： Kasper ck 徐群

1990年4月世界卫生组织(WHO)和世界血友病联盟(WFH)在日内瓦举行会议。来自7个国家的血友病治疗专家和WHO 的专家在会上讨论了血友病治疗的当代原则和寻求改进治疗的各种途径。下面是这次会议的结论和意见报告。一、结论血友病是一种伴... 详细信息

1990年4月世界卫生组织(WHO)和世界血友病联盟(WFH)在日内瓦举行会议。来自7个国家的血友病治疗专家和WHO 的专家在会上讨论了血友病治疗的当代原则和寻求改进治疗的各种途径。下面是这次会议的结论和意见报告。一、结论血友病是一种伴性遗传的出血性疾病,每十万个男孩中有15～20人发病,这个发病率在所调查的不同的种族和地域之间没有差异。因子Ⅷ或因子Ⅸ缺乏导致了过量的、持续的出血。从而引起血友病甲(占85%)或血友病乙(占15%)。严重的血友病人最常见的出血部位是在肢体大关节和肌肉。如果不及时输入所缺乏的因子来控制血友病人的出血,就会出现进行性的关节病和肌肉萎缩症。

关键词：血友病

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