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Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques

Light(Science & Applications) 2017年第1期6卷 201-209页

作者： Sara Mattana Maurizio Mattarelli Lorena Urbanelli Krizia Sagini Carla Emiliani mauro dalla serra Daniele Fioretto Silvia Caponi Department of Physics and Geology University of PerugiaPerugia I-06123Italy Laboratory of Biochemistry and Molecular Biology Department of ChemistryBiology and BiotechnologyUniversity of Perugiavia del GiochettoPerugia I-06123Italy CEMIN-Center of Excellence for Innovative Nanostructured Material Perugia I-06123Italy Istituto di Biofisica CNR(IBF-CNR) Unitàdi Trentoand FBKVia Sommarive 18Trento 38123Italy Istituto Officina dei Materiali del CNR(CNR-IOM)-Unitàdi Perugia c/o Department of Physics and GeologyUniversity of PerugiaPerugia I-06123Italy

Innovative label-free microspectroscopy,which can simultaneously collect Brillouin and Raman signals,is used to characterize the viscoelastic properties and chemical composition of living cells with sub-micrometric *** unprecedented statistical accuracy of the data combined with the high-frequency resolution and the high contrast of the recently built experimental setup permits the study of single living cells immersed in their buffer solution by contactless *** Brillouin signal is deconvoluted in the buffer and the cell components,thereby revealing the mechanical heterogeneity inside the *** particular,a 20%increase is observed in the elastic modulus passing from the plasmatic membrane to the nucleus as distinguished by comparison with the Raman spectroscopic *** line shape analysis is even more relevant for the comparison of cells under physiological and pathological *** oncogene expression,cells show an overall reduction in the elastic modulus(15%)and apparent viscosity(50%).In a proof-of-principle experiment,the ability of this spectroscopic technique to characterize subcellular compartments and distinguish cell status was successfully *** results strongly support the future application of this technique for fundamental issues in the biomedical field.

关键词： living single cell analysis mechanical properties Raman and Brillouin spectroscopy

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Probabilistic Distributions for <i>Acacia Mearnsii</i>De Wild Total Height and the Influence of Environmental Factors

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Journal of Applied Mathematics and Physics 2014年第3期2卷 1-10页

作者： Carlos Roberto Sanquetta Alexandre Behling Allan Libano Pelissari Ana Paula dalla Corte Sylvio Péllico Netto Augusto Arlindo Simon Department of Forest Science University Federal of Paraná Curitiba Brazil

This paper discusses the hypothesis of height distribution on a forest stand of Acacia mearnsii De Wild, known as black wattle. It remains constant at varied growing environments and, in addition, they are not influenced by age factor. The Wakeby equation was applied. The research was carried out in a black wattle stand at varied age levels and over two different agroecological regions where plantations are found: serra do Sudeste and Encosta do Sudeste, Rio Grande do Sul State, Brazil. It was observed that as the age rises there is an increase in the stand total height;while the number of trees decreases for the lower classes, it increases for the upper ones. This resulted in lengthening of the curve tail to the left and mode shift to the right, generating negative asymmetrical curves. Two types of height distribution were found: the sharp increase of probability in a specific class and some similar probabilities in successive classes. The distribution curves between the cultivation areas were statistically different and therefore the height distribution was dependent of environment.

关键词： Wakeby Distribution Forest Stand Age Cultivation Area Forest Structure

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