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Spatially resolved transcriptomics in immersive environments

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Visual Computing for Industry,Biomedicine,and Art 2022年第1期5卷 12-24页

作者： Denis Bienroth Hieu TNim Dimitar Garkov Karsten klein Sabrina Jaeger-Honz Mirana Ramialison Falk Schreiber Department of Computer and Information Science University of KonstanzKonstanzGermany Cell Biology Murdoch Children’s Research InstituteParkvilleMelbourneVICAustralia Australian Regenerative Medicine Institute Monash UniversityClaytonMelbourneVICAustralia Systems Biology Institute Australia ClaytonMelbourneVICAustralia Faculty of Information Technologies Monash UniversityMelbourneAustralia

Spatially resolved transcriptomics is an emerging class of high-throughput technologies that enable biologists to systematically investigate the expression of genes along with spatial *** data acquisition,one major hurdle is the subsequent interpretation and visualization of the datasets *** address this challenge,VR-Cardiomics is presented,which is a novel data visualization system with interactive functionalities designed to help biologists interpret spatially resolved transcriptomic *** implementing the system in two separate immersive environments,fish tank virtual reality(FTVR)and head-mounted display virtual reality(HMD-VR),biologists can interact with the data in novel ways not previously possible,such as visually exploring the gene expression patterns of an organ,and comparing genes based on their 3D expression ***,a biologist-driven use-case is presented,in which immersive environments facilitate biologists to explore and compare the heart expression profiles of different genes.

关键词： Spatially-resolved transcriptomics Spatial transcriptomics Virtual reality Fish tank virtual reality Head-mounted display Immersive analytics Immersive environment

Largest dinosaur tracksite in China (Cretaceous, Zhaojue area, Sichuan Province): On the verge of destruction

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Geoscience Frontiers 2021年第5期12卷 124-137页

作者： Lida Xing Martin G.Lockley Hendrik klein W.Scott Persons IV Guangzhao Peng Yong Ye Miaoyan Wang State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing 100083China School of the Earth Sciences and Resources China University of GeosciencesBeijing 100083China Dinosaur Trackers Research Group University of ColoradoDenver 80217USA Saurierwelt Paläontologisches Museum Alte Richt 7D-92318 NeumarktGermany Mace Brown Museum of Natural History Department of Geology and Environmental GeosciencesCollege of CharlestonCharleston 29401USA Zigong Dinosaur Museum ZigongSichuan ProvinceChina

The Zhaojue(ZJ)tracksites represent multiple steeply-inclined,track-bearing exposures in the clastic Feitianshan Formation of Sichuan Province that have been sequentially,excavated,mapped,expanded and destroyed,by quarrying,erosion or collapse,for almost three *** quarried area extends for more almost 1.0 km from north to south and-0.5 km from east to *** important track-bearing surfaces have been identified and identified as ZJ-Ⅰ,ZJ-Ⅱ,ZJ-ⅡN and ZJ-Ⅲ,some of which are newly exposed surfaces or expanded surfaces that were previously partially *** represent surfaces lost since they were first reported in papers published in *** ZJ-Ⅱ site represents a hugely expanded continuation of a much smaller track-bearing surface described in *** was mapped using drone technology,and now represents the largest tracksite in China,with a minimum count of 933 recognizable tracks,and the longest recorded sauropod and ornithopod trackways from China(-80 m and-52 m respectively),which also reveal a pronounced change in the direction of trackmaker *** ZJ-Ⅱ surface represents the best Zhaojue paleo-census sample giving a count of 68 individuals from 61 trackways(37 ornithopod,10 theropod,14 sauropod)and isolated tracks(representing 7 individuals).Thus,ornithopods(both large and small trackmakers)represent-54%of the total number of *** smaller ZJ-Ⅲ surface was mapped using traditional methods,and reveals at least 6 ornithopod-and 4 theropod *** the ZJ-Ⅱ and ZJ-Ⅲ surfaces reveal parallel ornithopod trackways suggestive of social or gregarious *** combined data from all four Zhaojue surfaces reveal a total of 1928 tracks,and include a few previously reported pterosaurs and theropod swim *** Zhaojue quarry complex provide a good example of multiple track-bearing sites(surfaces)that require long term study and monitoring to extract as much trackway data as possible before in situ physical evidence is lost.

关键词： Feitianshan Formation Onithopod Teropod Suropod

The Middle-Upper Jurassic dinosaur-pterosaur fauna in Shandong Province,China:Evidence from ichnology

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Journal of Palaeogeography 2023年第3期12卷 419-433页

作者： Li-Da Xing Martin G.Lockley Bo-Lin Tong Hendrik klein Chang Liu W.Scott Persons IV State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing100083China School of the Earth Sciences and Resources China University of GeosciencesBeijing100083China Dinosaur Trackers Research Group University of Colorado DenverPO Box 173364DenverCO80217USA Saurierwelt Palaontologisches Museum Alte Richt 7D-92318NeumarktGermany Mace Brown Museum of Natural History Department of Geology and Environmental GeosciencesCollege of CharlestonCharlestonSCUSA

Tracks previously examined between 2002 and 2015 from an incompletely-studied,and now partially lost tracksite site in the Santai Formation of Shandong Province were restudied based on new ex-cavations of additional *** contrast to the previous reports of tridactyl tracks,including Grallator,the present study discovered an additional assemblage of unequivocal pterosaur tracks(Pteraichnus)and a possible didactyl deinonychosaurian *** age of the Santai Formation is controversial but it is probably Late Jurassic,which corresponds to the epoch when pterosaur tracks appear *** interpretations,based on palynology and isotopic data,tentatively suggests an earlier Middle-Late Jurassic age and might suggest that the pterosaurian tracks are as old or somewhat older than any previously reported.

关键词： Santai Formation Theropod Deinonychosaur Pterosaur

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

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International Journal of Oral Science 2013年第3期5卷 121-129页

作者： Kyle B Jones Ophir D klein Program in Craniofacial and Mesenchymal Biology University of California Division of Medical Genetics Department of PediatricsUniversity of California Center for Craniofacial Anomalies Department of Orofacial Sciences University of California

The identification and characterization of stem cells is a major focus of developmental biology and regenerative *** advent of genetic inducible fate mapping techniques has made it possible to precisely label specific cell populations and to follow their progeny over *** combined with advanced mathematical and statistical methods,stem cell division dynamics can be studied in new and exciting *** advances in a number of tissues,relatively little attention has been paid to stem cells in the oral *** review will focus on current knowledge about adult oral epithelial stem cells,paradigms in other epithelial stem cell systems that could facilitate new discoveries in this area and the potential roles of epithelial stem cells in oral disease.

关键词： cancer stem cell invariant asymmetry neutral drift oral epithelial stem cell population asymmetry

Energy and spectral analysis of confined solar flares from radio and X-ray observations

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Research in Astronomy and Astrophysics 2021年第11期21卷 35-46页

作者： Cheng-Ming Tan Karl Ludwig klein Yi-Hua Yan Satoshi Masuda Bao-Lin Tan Jing Huang Guo-Wu Yuan CAS Key Laboratory of Solar Activity National Astronomical Observatories of Chinese Academy of SciencesBeijing 100101China School of Astronomy and Space Sciences University of Chinese Academy of SciencesBeijing 100049China Observatoire de Paris LESIA&Station de radioastronomie de Nanc¸ayUniv.PSLCNRSSorbonne Univ.Univ.de ParisUniv.d’Orl´eans5 place Jules JanssenF-92190 MeudonFrance Institute for Space-Earth Environmental Research Nagoya UniversityNagoya 464-8601Japan School of Information Science and Engineering Yunnan UniversityKunming 650091China

The energy and spectral shape of radio bursts may help us understand the generation mechanism of solar eruptions,including solar flares,coronal mass ejections,eruptive filaments,and various scales of *** different kinds of flares may have different characteristics of energy and spectral *** this work,we selected 10 mostly confined flare events during October 2014 to investigate their overall spectral behaviour and the energy emitted in microwaves by using radio observations from microwaves to interplanetary radio waves,and X-ray observations of GOES,RHESSI,and Fermi/*** found that:all the confined flare events were associated with a microwave continuum burst extending to frequencies of9.4~15.4 GHz,and the peak frequencies of all confined flare events are higher than 4.995 GHz and lower than or equal to 17 *** median value is around 9 *** microwave burst energy(or nuence)and the peak frequency are found to provide useful criteria to estimate the power of solar *** observations imply that the magnetic field in confined flares tends to be stronger than that in 412 flares studied by Nita et al.(2004).All 10 events studied did not produce detectable hard X-rays with energies above~300 keV indicating the lack of efficient acceleration of electrons to high energies in the confined flares.

关键词： Sun:solar flare Sun:radio burst Sun:energy Sun:spectral

Advanced protection against environmental degradation of silver mirror stacks for space application

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Journal of Materials Science & Technology 2021年第5期64卷 1-9页

作者： Rajendra Kurapati Vincent Maurice Antoine Seyeux Lorena H.klein Dimitri Mercier Gregory Chauveau Catherine Grees-Besset Loic Berthod Philippe Marcus PSL Research University CNRS-Chimie ParisTechInstitut De Recherche De Chimie ParisPhysical Chemistry of Surfaces Group11 Rue Pierre Et Marie Curie75005 ParisFrance CILAS MARSEILLE 600 Avenue De La Roche FourcadePole ALPHA Sud-Saint Mitre13400 AubagneFrance CNES Sous-direction Assurance QualitéService TechnologiesMatériaux Et Procédés18 AvenueÉdouard Belin31401 ToulouseFrance

Protection of silver mirror stacks from environmental degradation before launching is crucial for space ***,we report a comparative study of the advanced protection of silver mirror stacks for space telescopes provided by SiO_(2)and Al_(2)O_(3)coatings in conditions of accelerated aging by *** model silver stack samples were deposited by cathodic magnetron sputtering on a reference silica substrate for optical applications and a surface-pretreated SiC *** aging was per-formed in dry and more severe wet *** micrographic observations,surface and interface analysis by Time-of Flight Secondary Ion Mass Spectrometry(ToF-SIMS)and reflectivity measurements were combined to comparatively study the effects of *** results show a lower kinetics of degradation by accelerated aging of the stacks protected by the alumina coating in comparable test conditions.

关键词： Mirror layers Protection Layers Silver Atmospheric corrosion Accelerated aging Surface analysis Space applications

A new study from Mayo Clinic explores the possible benefits of standinq vs. sittinq

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空中英语教室（高级版．彭蒙惠英语） 2018年第8期 22-22,42,44页

作者： Traci klein

Partial Formalization: An Approach for Critical Analysis of Definitions and Methods Used in Bulk Extraction-Based Molecular Microbial Ecology

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Open Journal of Ecology 2015年第8期5卷 400-407页

作者： Donald A. klein Department of Microbiology Immunology and Pathology Campus Delivery Colorado State University Fort Collins CO USA

Partial formalization, which involves the development of deductive connections among statements, can be used to examine assumptions, definitions and related methodologies that are used in science. This approach has been applied to the study of nucleic acids recovered from natural microbial assemblages (NMA) by the use of bulk extraction. Six pools of bulk-extractable nucleic acids (BENA) are suggested to be present in a NMA: (pool 1) inactive microbes (abiotic-limited);(pool 2) inactive microbes (abiotic permissive, biotic-limited);(pool 3) dormant microbes (abiotic permissive, biotic-limited, but can become biotic permissive);(pool 4) in situ active microbes (the microbial community);(pool 5) viruses (virocells/virions/cryptic viral genomes);and (pool 6) extracellular nucleic acids including extracellular DNA (eDNA). Definitions for cells, the microbial community (in situ active cells), the rare biosphere, dormant cells (the microbial seed bank), viruses (virocells/virions/cryptic viral genomic), and diversity are presented, together with methodology suggested to allow their study. The word diversity will require at least 4 definitions, each involving a different methodology. These suggested definitions and methodologies should make it possible to make further advances in bulk extraction-based molecular microbial ecology.

关键词： Abiotic Envelope Baas Becking Biotic Envelope Bulk Extraction Cells Community Definitions Dormancy Methodology Microbial Diversity Microbial Seed Bank Natural Microbial Assemblages Partial Formalization Rare Biosphere

杂化化合物(C_(7)H_(11)N_(2))_(2)[CdCl_(4)]·0.5H_(2)O的晶体结构、光谱表征和光学性质

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无机化学学报 2022年第9期38卷 1834-1842页

作者： Fatma Garci Axel klein Hammouda Chebbi Mohamed Faouzi Zid University of Tunis El Manar Faculty of Sciences of TunisLaboratory of MaterialsCrystal Chemistry and Applied ThermodynamicsTunisTunisia University of Cologne Faculty of Mathematics and Natural SciencesDepartment of ChemistryInstitute for Inorganic ChemistryCologneGermany University of Tunis Preparatory Institute for Engineering Studies of TunisTunisTunisia

开发了标题化合物(C_(7)H_(11)N_(2))_(2)[CdCl_(4)]·0.5H_(2)O(C_(7)H_(11)N_(2)=4-(二甲基氨基)吡啶鎓)的制备程序,并得到良好的收率和纯度。Cd离子在略微扭曲的四面体环境中与4个氯离子配位。晶体排列显示出层状结构,有机层和无机... 详细信息

开发了标题化合物(C_(7)H_(11)N_(2))_(2)[CdCl_(4)]·0.5H_(2)O(C_(7)H_(11)N_(2)=4-(二甲基氨基)吡啶鎓)的制备程序,并得到良好的收率和纯度。Cd离子在略微扭曲的四面体环境中与4个氯离子配位。晶体排列显示出层状结构,有机层和无机层交替排列,平行于(001)平面,位于x=n+1/2(n∈Z)。在晶体中,有机层和无机层通过C—H…Cl、C—H…O、N—H…Cl和N—H…O氢键相互作用连接。Hirshfeld表面分析和结构的指纹图表明分子堆积受氢键和π堆积的控制。UV-Vis漫反射光谱使我们能够用Tauc外推法确定具有半导体特性的3.596 eV的直接带隙。观察到的在562 nm处具有最大值的光致发光带归属于4-(二甲基氨基)吡啶鎓阳离子中的激发π-π*态。

关键词：氯镉(Ⅱ)配离子 4-二甲氨基吡啶鎓晶体结构 Hirshfeld表面分析光致发光