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Urine exosome mRNA-based test for monitoring kidney allograft rejection:Effects of sample transportation and storage,and interference substances

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World Journal of Methodology 2023年第5期13卷 492-501页

作者： matt mcfaul Chris Ventura Sean Evans Halil Dundar Marc J Rumpler Christopher McCloskey Dave Lowe Alexandre V Vlassov Department of Research and Development Thermo Fisher ScientificWest HillsCA 91304United States

BACKGROUND Exosomes are 30-150 nm nanovesicles with sophisticated nucleic acids cargo,actively secreted by all cells within human body,and found in abundance in all body fluids,including *** extracellular vesicles have tremendous potential for next generation diagnostics,theoretically enabling noninvasive assessment of organ and tissue function via liquid biopsy *** Recently,feasibility of an exosomal molecular test was demonstrated for postorgan transplant monitoring:Analysis of urine-derived exosomal mRNA cargo allowed early detection of kidney allograft ***,we further studied urine-derived exosomes and their mRNA content as a highly promising diagnostic *** included stability studies of urine samples and exosomal mRNA upon transportation from the point of collection to a centralized testing facility,short-term storage of urine at different conditions upon receipt till the point molecular assay is performed,and effects of various potentially interfering substances on the downstream quantitative polymerase chain reaction(qPCR)*** The urine specimens were stored at various conditions and pre-processed in different ***,samples were passed through the columns to capture all extracellular vesicles,the vesicles were lysed to release their content and the exosomal RNA was purified on the mini-columns,reverse transcription was performed,next pre-amplification,followed by a qPCR analysis for a panel of RESULTS To ensure exosomal RNA integrity,the harvested urine specimens should be shipped refrigerated,by overnight *** can next be stored at the test site for up to 1 wk at 4°C,and long term should be frozen at-80°*** specimens must be centrifuge at low G-force to deplete cells and debris,to ensure consistent top results in downstream molecular *** commonly used medications(tacrolimus,cyclosporin A,mycophenolic acid,everolimus,sirolimus,ascomycin,teriflunomide)were tested and confirmed that they do n

关键词： Kidney allograft Post-transplant monitoring Liquid biopsy Exosome mRNA Quantitative polymerase chain reaction

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Fitness Sharing Chaotic Particle Swarm Optimization (FSCPSO): A Metaheuristic Approach for Allocating Dynamic Virtual Machine (VM) in Fog Computing Architecture

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Computers, Materials & Continua 2024年第8期80卷 2557-2578页

作者： Prasanna Kumar Kannughatta Ranganna Siddesh Gaddadevara matt Chin-Ling Chen Ananda Babu Jayachandra Yong-Yuan Deng Department of Computer Science&Engineering Ramaiah Institute of TechnologyBangalore560054India Department of Artificial Intelligence&Data Science Ramaiah Institute of TechnologyBangalore560054India School of Information Engineering Changchun Sci-Tech UniversityChangchun130600China Department of Computer Science and Information Engineering Chaoyang University of TechnologyTaichung41349Taiwan Department of Information Science and Engineering Malnad College of EngineeringHassan573202India

In recent decades,fog computing has played a vital role in executing parallel computational tasks,specifically,scientific workflow *** cloud data centers,fog computing takes more time to run workflow ***,it is essential to develop effective models for Virtual Machine(VM)allocation and task scheduling in fog computing *** task scheduling,VM migration,and allocation,altogether optimize the use of computational resources across different fog *** process ensures that the tasks are executed with minimal energy consumption,which reduces the chances of resource *** this manuscript,the proposed framework comprises two phases:(i)effective task scheduling using a fractional selectivity approach and(ii)VM allocation by proposing an algorithm by the name of Fitness Sharing Chaotic Particle Swarm Optimization(FSCPSO).The proposed FSCPSO algorithm integrates the concepts of chaos theory and fitness sharing that effectively balance both global exploration and local *** balance enables the use of a wide range of solutions that leads to minimal total cost and makespan,in comparison to other traditional optimization *** FSCPSO algorithm’s performance is analyzed using six evaluation measures namely,Load Balancing Level(LBL),Average Resource Utilization(ARU),total cost,makespan,energy consumption,and response *** relation to the conventional optimization algorithms,the FSCPSO algorithm achieves a higher LBL of 39.12%,ARU of 58.15%,a minimal total cost of 1175,and a makespan of 85.87 ms,particularly when evaluated for 50 tasks.

关键词： Fog computing fractional selectivity approach particle swarm optimization algorithm task scheduling virtual machine allocation

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Symptom presentation and evolution in the first 48 hours after injury are associated with return to play after concussion in elite Rugby Union

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Journal of Sport and Health Science 2024年第3期13卷 387-397页

作者： Ross Tucker matt Cross Keith Stokes Lindsay Starling Rosy Hyman Simon Kemp Stephen West Martin Raftery Eanna Falvey James Brown Institute of Sport and Exercise Medicine(ISEM) Department of ExerciseUniversity of StellenboschTygerberg 7505South Africa World Rugby Ltd. Dublin D02 AE93Ireland Premiership Rugby London SW1V 1PXUK Centre for Health and Injury and Illness Prevention in SportUniversity of BathClaverton Down BA27AYUK UK Collaborating Centre on Injury and Illness Prevention in Sport(UKCCIIS) University of BathClaverton Down BA27AYUK Rugby Football Union London TW27BAUK London School of Tropical Medicine London WC1E 7HTUK Sport Injury Prevention Research Centre Faculty of KinesiologyUniversity of CalgaryCalgary T2N 1N4Canada Department of Medicine School of Medicine and HealthUniversity College CorkCork T12 EC8PIreland Carnegie Applied Rugby Research(CARR)centre Carnegie School of SportLeeds Beckett UniversityLeeds LS63QSUK

Background:Return to play(RTP)in elite rugby is managed using a 6-stage graduated RTP protocol,which can result in clearance to play within 1 week of *** aimed to explore how symptom,cognitive,and balance presentation and evolution during concussion screens 2 h(head injury assessment(HIA2)and 48 h(HIA3)after injury were associated with time to RTP)to identify whether a more conservative graduated RTP may be ***:A retrospective cohort study was conducted in 380 concussed rugby players from elite men’s rugby over 3 consecutive *** were classified as shorter or longer returns,depending on whether RTP occurred within 7 days(allowing them to be considered to play the match 1 week after injury)or longer than 8 days,***,cognitive,and balance performance during screens was assessed relative to baseline(normal or abnormal)and to the preceding screen(improving or worsening).Associations between sub-test abnormalities and RTP time were explored using odds ratios(OR,longer ***).Median day absence was compared between players with abnormal or worsening results and those whose results were normal or ***:Abnormal symptom results during screens 2 h and 48 h after concussion were associated with longer return time(HIA2:OR=2.21,95%confidence interval(95%CI):1.39-3.50;HIA3:OR=3.30,95%CI:1.89-5.75).Worsening symptom number or severity from the time of injury to 2 h and 48 h post-injury was associated with longer return(HIA2:OR=2.49,95%CI:1.36-4.58;HIA3:OR=3.34,95%CI:1.10-10.15).Median days absence was greater in players with abnormal symptom results at both HIA2 and *** and balance performance were not associated with longer return and did not affect median days ***:Symptom presentation and evolution within 48 h of concussion were associated with longer RTP *** may guide a more conservative approach to RTP,while still adhering to individualized concussion management principles.

关键词： Brain injury Concussion management General return to play Medical management

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Rare Case of Large Anterior Meningocele Associated with Uterine Didelphys

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Open Journal of Modern Neurosurgery 2024年第2期14卷 104-107页

作者： Joseph Maalouly matt Wilson Sanjay Adusumilli Vijidha Rajkumar Shanu Gambhir Department of Neurosurgery Norwest Private Hospital Bella Vista Australia Department of Obstetrics and Gynecology Norwest Private Hospital Bella Vista Australia Department of General Surgery Norwest Private Hospital Bella Vista Australia

We report a 27-year-old female who presented with abdominal distension, saddle anesthesia, and lower back pain. CT and MRI of the abdomen/spine were performed which showed a large anterior sacral meningocele occupying most of the pelvic and abdominal cavity and displacement of their respective contents. Anterior approach was chosen, uterine didelphys was identified and mobilized, meningocele identified and gentle drainage with subsequent resection of the cyst wall and watertight closure was achieved. Subsequent MRI demonstrated resolution of most the sacral meningocele. Patient symptoms improved and are doing well.

关键词： Sacral Agenesis Curarino Triad Large Meningocele

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中国手斧技术演化:新进展和未来展望

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Science Bulletin 2024年第14期69卷 2161-2165页

作者：李浩王社江 matt G.Lotter Robin Dennell Kathleen Kuman 陈发虎 State Key Laboratory of Tibetan Plateau Earth System Resources and Environment(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China Key Laboratory of Vertebrate Evolution and Human Origins Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of SciencesBeijing 100044China Palaeo-Research Institute University of JohannesburgAuckland Park 2006South Africa Department of Archaeology University of ExeterExeter EX44QEUK School of Geography Archaeology and Environmental StudiesUniversity of the WitwatersrandJohannesburg WITS 2050South Africa

Handaxes are often regarded as the"tool-type-specimenoof the Acheulean techno-complex,not only because they are a very common artefact but because they contain rich technological,behavioral,and cognitive data that have been used to highlight trends in human evolution and lithic production *** this end,the handaxe is the earliest tool possessing consistent morphological features,in particular,the appearance of a symmetrical concept in handaxe shape when compared with other Acheulean large shaping tools(e.g.,cleavers),and their manufacture is widely regarded as a significant milestone in the evolution of lithic technology,indicating improvements in tool-making hominins’abilities for complex thought and cognition.

关键词：技术演化未来展望 earliest

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Improved Analytical Methods for Determination of Residues of Nitrapyrin and 6-Chloropicolinic Acid in Different Crop Matrices by Liquid Chromatography-Tandem Mass Spectrometry

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Journal of Agricultural Chemistry and Environment 2024年第3期13卷 263-281页

作者： Fabiola G. Zuno-Floriano Riza L. Reyes-Punongbayan matt J. Hengel Department of Environmental Toxicology University of California Davis California USA

Novel analytical methods were developed for the determination of nitrapyrin and 6-chloropicolinic acid (6-CPA) residues in agricultural products. Nitrapyrin was extracted with hexane: toluene (1:1, v/v) and 6-CPA with 0.1 N sodium hydroxide (NaOH). For nitrapyrin, samples were cleaned with silica solid phase extraction (SPE) and for 6-CPA with C18 or carbon SPE. Analysis was performed by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) with external calibration (0.010 to 0.0010 µg∙mL−1 for nitrapyrin and 0.0050 to 0.00025 µg∙mL−1 for 6-CPA). Recoveries for nitrapyrin and 6-CPA were 68% - 102% and 66% - 90%, respectively. Nitrapyrin was detected in celery and onion (−1) and 6-CPA in onion, mustard greens and lettuce (−1). Lower level method validation (LLMV) for nitrapyrin and 6-CPA was 0.020 µg∙g−1 and 0.050 µg∙g−1, respectively. The methods presented in this study are robust and were utilized for the registration of nitrapyrin on agricultural commodities in the United States.

关键词： Herbicide Agricultural Products External Calibration LC-MS/MS Registration

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A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

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Insect Science 2024年第4期31卷 1285-1295页

作者： Melissa L.Starkie Elizabeth V.Fowler Alexander M.Piper Xiaocheng Zhu Pauline Wyatt David Gopurenko matt N.Krosch Francesca Strutt Karen F.Armstrong Hamish Patrick Mark K.Schutze Mark J.Blacket Department of Agriculture and Fisheries BiosecurityBrisbaneQueenslandAustralia Agriculture Victoria AgriBioBundooraVictoriaAustralia Department of Primary Industries Wagga Wagga Agricultural InstituteWagga WaggaNew South WalesAustralia Forensic Services Group Queensland Police ServiceBrisbaneQueenslandAustralia Department of Pest-management and Conservation Lincoln UniversityLincolnNew Zealand Better Border Biosecurity(B3 B3nz.org.nz)LincolnNew Zealand

Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host *** need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference *** morphologically similar,adults may be separated based on subtle characters;however,some characters exhibit intraspecific variability,creating overlap between the two ***,there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between *** and ***;therefore,ambiguous samples remain *** we report the first molecular marker that can consistently distinguish between *** and *** diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene *** confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation system(ARMS)PCR assay for rapid diagnosis of the *** assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in *** and the other targeting adjacent SNPs present in ***(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly ***,this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses.

关键词： ARMS-PCR colony molecular diagnostics pangolin tetra-primer

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温暖的混凝土奥地利Jägerbau集团新总部大楼

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室内设计与装修 2024年第6期 42-45页

作者： [奥地利]Innauer matt Architekten Innauer matt Architekten

Jägerbau集团新总部大楼坐落于奥地利布鲁登茨。该建筑整合了邻近区域,并成为当地城市语境中的一个组成部分。建筑独特的红褐色体量塑造了适合的城市空间,呼应复杂环境的同时,也支持了Her-rengasse和Zürcherstrasse这2条街道沿线未来... 详细信息

Jägerbau集团新总部大楼坐落于奥地利布鲁登茨。该建筑整合了邻近区域,并成为当地城市语境中的一个组成部分。建筑独特的红褐色体量塑造了适合的城市空间,呼应复杂环境的同时,也支持了Her-rengasse和Zürcherstrasse这2条街道沿线未来的发展。此外,总部大楼还体现着它在卢登斯城市景观中的公共意义:这是一座开放、结构灵活的迷人建筑,它明确地向这个城市传达着Jägerbau集团作为一家科技公司的身份特征。

关键词：身份特征总部大楼城市景观邻近区域城市语境奥地利

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让爱传递——吉姆和凯西·迪尔

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中国花卉园艺 2024年第8期 24-26页

作者：韶月(译) matt Mcclellan 不详

吉姆(Jim Deal)和凯西·迪尔(Kathy Deal)的故事开端与大多数HILA获奖者不同。吉姆最初进入苗圃行业,是因为厌倦了粉刷房屋的工作。吉姆毕业于北卡罗来纳州立大学,拥有野生动物生物学学位。为了完成学业,他从事房屋粉刷业务,对如何做生... 详细信息

吉姆(Jim Deal)和凯西·迪尔(Kathy Deal)的故事开端与大多数HILA获奖者不同。吉姆最初进入苗圃行业,是因为厌倦了粉刷房屋的工作。吉姆毕业于北卡罗来纳州立大学,拥有野生动物生物学学位。为了完成学业,他从事房屋粉刷业务,对如何做生意有了一定了解,但他不想一辈子粉刷房屋。

关键词：凯西迪尔野生动物生物学吉姆学位完成学业获奖者

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网络安全事件管理和响应指南

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中国教育网络 2024年第2期 76-77页

作者： Christine Whalley matt Kenslea Asha Ramachandra Steve Fletcher 董明(译) 不详

确保整个团队了解要采取什么行动,可以对机构如何以及多快地从一个安全事件中恢复产生重要影响事件管理和响应是任何网络安全计划的关键要素。每一个事件都提供了教训和改进的机会,高等教育领导者永远不应该假设类似的事件不会再次发生... 详细信息

确保整个团队了解要采取什么行动,可以对机构如何以及多快地从一个安全事件中恢复产生重要影响事件管理和响应是任何网络安全计划的关键要素。每一个事件都提供了教训和改进的机会,高等教育领导者永远不应该假设类似的事件不会再次发生。本指南涵盖了在事件发生之前、期间和之后应该做些什么,以确保机构通过有效的响应、恢复和持续改进保持更好的弹性。事件发生前制定事件响应计划:制定详细的事件响应计划,概述检测过程、报告和响应安全事件的程序。

关键词：事件响应安全事件检测过程高等教育持续改进关键要素指南网络安全计划

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