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Cystatin C Peptide Effects on B16F10 Melanoma Cells

CellBio 2021年第1期10卷 1-9页

作者： mathieu hill James L. Cox AT Still University Department of Biochemistry West Jefferson Kirksville Missouri USA

Background: Metastasis is a major problem for effective therapy of cancer. Small cysteine protease inhibitors, cystatins have been shown to be anti-metastatic for a number of different cancers. We have identified a small peptide of cystatin which exhibits anti-cancer properties for B16 melanoma cells in vitro. Methods: B16 melanoma cells were measured for growth, proliferation, migration, and apoptosis in the presence and absence of cystatin peptide. Results: The cystatin peptide reduced melanoma cell growth, proliferation, migration, and increased apoptosis in melanoma cells in culture. Conclusion: Cystatin peptide exhibits anti-cancer effects on highly metastatic B16 melanoma cells in culture.

关键词： Cystatin Peptide Melanoma Metastasis

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Conserved Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation

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Molecular Plant 2015年第12期8卷 1751-1765页

作者： Zhenhua Liu Benoit Boachon RaphaelLugan Raquel Tavares mathieu Erhardt Jerome Mutterer Valerie Demais Stephanie Pateyron Veronique Brunaud Toshiyuki Ohnishi Ales Pencik Patrick Achard Fan Gong Peter Hedden Daniele Werck-Reichhart Hugues Renault Institute of Plant Molecular Biology Centre National de la Recherche Scientifique (CNRS) University of Strasbourg 67084 Strasbourg France Laboratoire de Biometrie et Biologie Evolutive Universite Lyon 1 CNRS 69622 Villeurbanne France plateforme d'lmagerie In Vitro IFR 37 de Neurosciences 67084 Strasbourg France Transcriptomic Platform Unite de Recherche en Genomique Vegetale (URGV) INRA Universite d'Evry Val d'Essonne CNRS 91057 Evry France Bioinformatics for Predictive Genomics URGV INRA Universite d'Evry Val d'Essonne CNRS 91057 Evry France Graduate School of Agriculture Shizuoka University Shizuoka 422-8529 Japan Laboratory of Growth Regulators & Department of Chemical Biology and Genetics Centre of the Region Hana for Biotechnological and Agricultural Research Faculty of Science Palacky University & Institute of Experimental Botany AS CR 771 47 Olomouc Czech Republic Rothamsted Research Harpenden Hertfordshire AL5 2JQ UK University of Strasbourg Institute for Advanced Study (USIAS) 67084 Strasbourg France Freiburg institute for Advanced Studies (FRIAS) University of Freiburg 79104 Freiburg Germany Present address: 158 Emerson Hall Section of Plant Biology School of Integrative Plant Science Cornell University Ithaca NY 14853 USA present address: Laboratoire Physiologie des Fruits et Legumes - EA 4279 Campus Agroparc Avignon France present address: Home Office Science - Centre for Applied Science and Technology Woodcock Hill Sandridge St Albans HeFts AL4 9HQ UK

Global inspection of plant genomes identifies genes maintained in low copies across taxa and under strong purifying selection, which are likely to have essential functions. Based on this rationale, we investigated the function of the low-duplicated CYP715 cytochrome P450 gene family that appeared early in seed plants and evolved under strong negative selection. Arabidopsis CYP715A 1 showed a restricted tissue-specific expres- sion in the tapetum of flower buds and in the anther filaments upon anthesis, cyp715a1 insertion lines showed a strong defect in petal development, and transient alteration of pollen intine deposition. Comparative expres- sion analysis revealed the downregulated expression of genes involved in pollen development, cell wall biogenesis, hormone homeostasis, and floral sesquiterpene biosynthesis, especially TPS21 and several key genes regulating floral development such as MYB21, MYB24, and MYC2. Accordingly, floral sesquiterpene emission was suppressed in the cyp715a1 mutants. Flower hormone profiling, in addition, indicated a modi- fication of gibberellin homeostasis and a strong disturbance of the turnover of jasmonic acid derivatives. Petal growth was partially restored by the active gibberellin GA3 or the functional analog of jasmonoyl-isoleucine, coronatine. CYP715 appears to function as a key regulator of flower maturation, synchronizing petal expan- sion and volatile emission. It is thus expected to be an important determinant of flower-insect interaction.

关键词： flower development phylogenomics negative selection jasmonate gibberellins volatile compounds

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