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Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of <i>Pleurotus flabellatus</i>Using Response Surface Methodology

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Advances in Bioscience and Biotechnology 2015年第6期6卷 419-426页

作者： Shaiful Azuar Mohamad mat rasol awang Rosnani Abdul Rashid Liew Siew Ling Fauzi Daud Aidil Abdul Hamid Rahayu Ahmad Wan Mohtar Wan Yusoff Agrotechnology and Biosciences Division Malaysian Nuclear Agency Bangi Malaysia Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Malaysia

The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. The central composite design was applied to study the significant factors and the interactions between the chosen factors, if present. The Design Expert software generated 20 runs. The optimized conditions obtained were as follows: the agitation rate of 129.8 rpm, incubation temperature at 27.8°C, and initial pH of 6.06. The optimized conditions tripled the productivity at the range of 980 - 1040 mg/litre/day compared to the initial rate productivity at 310 mg/litre/day. From the quadratic equation,the agitation rate, temperature and the interaction between agitation rate and temperature were found to be significant (p mate.

关键词： Submerged Culture Fermentation Response Surface Methodology Mushroom Production

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Production of Endopolysaccharides from Malaysia’s Local Mushrooms in Air-Lift Bioreactor

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Advances in Bioscience and Biotechnology 2015年第7期6卷 456-462页

作者： Shaiful Azuar Mohamad mat rasol awang Rusli Ibrahim Choong Yew Keong Mohd Yusof Hamzah Rosnani Abdul Rashid Sobri Hussein Khairuddin Abdul Rahim Fauzi Daud Aidil Abdul Hamid Wan Mohtar Wan Yusoff Agrotechnology and Biosciences Division Malaysian Nuclear Agency Bangi Malaysia Herbal Medicine Research Centre Institute For Medical Research Kuala Lumpur Malaysia Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Malaysia

Four local mushroom species, viz. Auricularis polytricha, Lentinus edodes, Agrocybe sp and Pleurotus flabellatus were grown under submerged culture and screened for endopolysaccharides. The fermentation was done in 250 ml working volume Erlenmeyer flask and the fermentation curves for all species were established. Pleurotus flabellatus has the highest rate of biomass production at the rate of 0.180 g/L/day, at 10 days hence chosen for further investigation. Two additional media, viz. Mushroom Complete Media (MCM) and Yeast Malt (YM) were selected to be compared with potato extract(PE) media used initially. MCM media produced the highest biomass productivity at the rate of 0.311 g/L/day. Pleurotus flabellatus biomass was extracted using modified Mizuno method and the endopolysaccharide obtained was tested for β-glucan. The yield of β-glucan was 7.70 ± 1.11 g/100g. The polysaccharides were purified using column chromatography to yield four fractions. The fourth fraction F4, gave the highest molecular weight at 3.058 × 106 Dalton (11.8%) and 1.282 × 104 Dalton (88.2%). The mushroom, P. flabbelatus was cultured using air-lift bioreactor, and the highest productivity was obtained at air-flowrate 2 L/min, yielding 2.25 g/L/day. The yield of biomass against substrate used (glucose consumption) Yb/s was 0.78 g/g.

关键词： Submerged Culture Fermentation Mushroom β-Glucan Column Chromatography Molecular Weight

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