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The 8th Asia/Oceania Regional Congress of Gerontology and Geriatrics

作者： Taro Kojima Masahiro Akishita Tetsuro Nakamura Kazushi Nomura Yumiko Ohike Sumito Ogawal Katsuya Iijima masato eto Yasuyoshi Ouchi Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Research Institute of Aging Science Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo Department of Geriatric Medicine Graduate School of Medicine The University of Tokyo

＜正＞Purpose:To identify the risks of falls among the elderly outpatients,fall risk and associated factors were examined in a cross-sectional study design. Method:We enrolled 259 outpatients（mean 76 years-old,male ... 详细信息

关键词： Fall polypharmacy hypertension hypnotic

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Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential

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World Journal of Stem Cells 2017年第10期9卷 179-186页

作者： Harumichi Itoh Shimpei Nishikawa Tomoya Haraguchi Yu Arikawa Shotaro eto masato Hiyama Toshie Iseri Yoshiki Itoh Munekazu Nakaichi Yusuke Sakai Kenji Tani Yasuho Taura Kazuhito Itamoto Department of Small Animal Clinical Science Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Surgery Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Radiology Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Pathology Joint Faculty of Veterinary Medicine Yamaguchi University

AIMTo identify and characterize functionally distinct subpopulation of adipose-derived stem cells (ADSCs). METHODSADSCs cultured from mouse subcutaneous adipose tissue were sorted fluorescence-activated cell sorter based on aldehyde dehydrogenase (ALDH) activity, a widely used stem cell marker. Differentiation potentials were analyzed by utilizing immunocytofluorescece and its quantitative analysis. RESULTSApproximately 15% of bulk ADSCs showed high ALDH activity in flow cytometric analysis. Although significant difference was not seen in proliferation capacity, the adipogenic and osteogenic differentiation capacity was higher in ALDHHi subpopulations than in ALDHLo. Gene set enrichment analysis revealed that ribosome-related gene sets were enriched in the ALDHHi subpopulation. CONCLUSIONHigh ALDH activity is a useful marker for identifying functionally different subpopulations in murine ADSCs. Additionally, we suggested the importance of ribosome for differentiation of ADSCs by gene set enrichment analysis.

关键词： Adipose-derived stem/stromal cell Aldehyde dehydrogenase activity Flow cytometry Subpopulation Ribosome

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