检索结果-南通市图书馆

Development of a competitive enzyme-linked immunosorbent assay for therapeutic drug monitoring of afatinib

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2019年第1期9卷 49-54页

作者： Rintaro Sogawa Tetsuya Saita Yuta Yamamoto Sakiko Kimura Yutaka Narisawa Shinya Kimura masashi Shin Department of Pharmacy Saga University Hospital Applied Life Science Department Faculty of Biotechnology and Life Science Sojo University Division of Hematology Respiratory Medicine and Oncology Department of Internal Medicine Faculty of Medicine Saga University

Afatinib is an oral tyrosine kinase inhibitor(TKI) approved for treating advanced non-small cell lung cancer. It is necessary to develop a simple quantification method for TKIs in order to facilitate therapeutic drug monitoring(TDM) in clinical settings. This study sought to develop a simple and sensitive competitive enzyme-linked immunosorbent assay(ELISA) to quantify afatinib in plasma for routine pharmacokinetic applications. An anti-afatinib antibody was obtained using(S)-N-4-(3-chloro-4-fluorophenyl)-7-(tetrahydrofuran-3-yloxy)-quinazoline-4,6-diamine(CTQD), which has the same substructure as afatinib, as a hapten. Enzyme labeling of afatinib with horseradish peroxidase was similarly performed using CTQD. A simple competitive ELISA for afatinib was developed based on the principle of direct competition between afatinib and the enzyme marker for the anti-afatinib antibody, which had been immobilized on the plastic surface of a microtiter plate. Plasma afatinib concentrations below the limit of quantification of 30 pg/mL were reproducibly measurable. Also, the values of plasma afatinib levels measured from 20 patients were comparable with those measured by high-performance liquid chromatography, and there was a strong correlation between the values determined by both methods(Y=0.976 X – 0.207, r=0.975). As indicated by its specificity and sensitivity, this newly developed ELISA for afatinib is an important tool for TDM and studies of the pharmacokinetics of afatinib.

关键词： Afatinib Enzyme-linked immunosorbent assay Therapeutic drug monitoring Tyrosine-kinase inhibitor

Karyotypes of field mice of the genus Apodemus (Mammalia： Rodentia) from China

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2018年第5期39卷 348-355页

作者： Masaharu Motokawa Yi Wu masashi Harada Yuta Shintaku Xue-Long Jiang Yu-Chun Li Kyoto University Museum Kyoto University Kyoto 606-8501 Japan Key Laboratory of Conservation and Application in Biodiversity of South China School of Life Sciences Guangzhou University Guangzhou Guangdong 510006 China Laboratory Animal Center Graduate School of Medicine Osaka City University Osaka 545-8585 Japan Wildlife Research Center Kyoto University Sakyo Kyoto 606-8203 Japan Japan Monkey Centre Inuyama Aichi 484-0081 Japan Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Marine College Shandong University(Weihai) Weihai Shandong 264209 China

Karyotypes of four Chinese species of field mice of the genus Apodemus were examined,including Apodemus chevrieri （diploid chromosome number,2n=48,fundamental number of autosomal arms,FNa=56）,*** （2n=48,FNa=48）,*** （2n=48,FNa=48）,and *** （2n=48,FNa=48）.Karyotypes of ***,***,and *** are reported here for the first time,providing useful information for their species *** the karyotypes of all species of Apodemus in Asia,both in this and previous studies,provides a solid overview of the chromosome evolution and species differentiation of the genus in East *** addition to allopatric speciation,chromosome rearrangements likely played an important role in the formation of the four Apodemus species groups as well as speciation within each group in East *** example,increased centromeric heterochromatin in *** may have contributed to the post-mating reproductive isolation from the *** clade.

关键词： Karyotype Chromosome evolution Speciation Taxonomy Field mice

Flight simulation from takeoff to yawing of eVTOL airplane with coaxial propellers by fluid-rigid body interaction

在线全文

学校读者我要写书评

暂无评论

Advances in Aerodynamics 2023年第1期5卷 29-43页

作者： Ritsuka Gomi Ayato Takii masashi Yamakawa Shinichi Asao Seiichi Takeuchi Momoha Nishimura Department of Mechanical Engineering Kyoto Institute of TechnologyKyotoJapan Department of Mechanical Engineering College of Industrial TechnologyHyogoJapan

In this study,we adopt a coupled fluid-rigid body simulation using the moving computational domain method and multi-axis sliding mesh method for the takeoff,hovering,and yawing flight of an electric vertical takeoff and landing aircraft(eVTOL).The aircraft model has four pairs of coaxial propellers,and the computational domain is divided into three domains to move the aircraft and eight propeller domains to rotate the *** a result,we clarify the behavior and aerodynamic force of the aircraft when the input values are determined by the automatic *** results in the flow field also show that the downwash spreads in a crisscross pattern on the ground,the wind reaches different ranges on the ground depending on the flight altitude,and that the coaxial propeller causes an asymmetry in the velocity field during ***-quently,we conclude that this method is effective for the flight simulation of an eVTOL.

关键词： Computational fluid dynamics Moving grid Coupled simulation Flying car eVTOL

One-year outcomes and predictable factors for microhook ab interno trabeculotomy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2022年第4期15卷 598-603页

作者： Daiki Sakai masashi Fujihara Satoshi Yokota Makoto Nakamura Yasuo Kurimoto Department of Ophthalmology Kobe City Eye HospitalKobe 650-0047Japan Department of Ophthalmology Kobe City Medical Center General HospitalKobe 650-0047Japan Department of Surgery Division of OphthalmologyKobe University Graduate School of MedicineKobe 650-0017Japan

AIM: To investigate the one-year outcomes and factors that influence the results of microhook ab interno trabeculotomy(μLOT). METHODS: The medical records of consecutive patients with open angle glaucoma who underwent μLOT(including combination of μLOT and cataract surgery) between Februar y 2018 and July 2019 were retrospectively reviewed. Surgical success was defined as the following: an intraocular pressure(IOP)≤21 mm Hg or IOP≤preoperative IOP with a reduced number of glaucoma eye drops, without additional glaucoma surgery, and assessed using KaplanMeier survival analysis. A multivariate Cox proportionalhazards regression model was used to investigate the factors associated with surgical ***: The 59 eyes of 59 patients comprising 28 eyes with primary open angle glaucoma(POAG) and 31 with secondary open angle glaucoma(SOAG) were included. The mean IOP and number of glaucoma eye drops significantly decreased from 25.3±7.2 mm Hg and 3.9±1.1, preoperatively to 16.1±4.4 mm Hg(P25 mm Hg within 2 wk post-surgery;P<0.001, a HR: 5.382, 95%CI:2.113-13.707) as independent factors associated with surgical ***: The μLOT is a good treatment option for POAG eyes. However, the postoperative course should be carefully followed in cases with postoperative IOP spike.

关键词： microhook ab interno trabeculotomy trabeculotomy ab interno minimally invasive glaucoma surgery survival analysis

Simulation of Fast-Recovery Cross-Modulation Characteristics in Semiconductor Optical Amplifier with Assist Light

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

光学学报 2003年第S1期23卷 453-454页

作者： Atsushi Matusmoto Kosuke Nishimura Katsuyuki Utaka masashi Usami School of Science and Engineering Waseda University 3-4-1 Ohkubo Shinjuku Tokyo 169-8555 JapanKDDI R&D Laboratories Inc. 2-1-15 Ohara Kamifukuoka Saitama 356-8502 JapanSchool of Science and Engineering Waseda University 3-4-1 Ohkubo Shinjuku Tokyo 169-8555 JapanKDDI R&D Laboratories Inc. 2-1-15 Ohara Kamifukuoka Saitama 356-8502 Japan

We analyzed the characteristics of cross-modulations (XM) and their recovery times in a semiconductor optical amplifier by a newly-developed TMM. The calculated results suggest faster recovery of the XMs by introducin... 详细信息

关键词： Simulation of Fast-Recovery Cross-Modulation Characteristics in Semiconductor Optical Amplifier with Assist Light as for of XPM SOA in with

Asian international grid connection and potentiality of DC superconducting power transmission

在线全文

学校读者我要写书评

暂无评论

Global Energy Interconnection 2018年第1期1卷 11-19页

作者： Satarou Yamaguchi Takashi Iitsuka masashi Osada Ryuichi Yokoyama Chubu University Matsumoto-cho 1200Kasugai487-8500AichiJapan JGC Corporation Minato Mirai 2-3-1Nishi-kuYokohama 220-6001Japan ABB K.K. Ohsaki 2-1-1Shinagawa-ku141-6022 TokyoJapan Energy and Environment Technology Research Institute 1-22-2 Nishiwaseda Shinjuku169-0051 TokyoJapan

In 2011, a large scale earthquake and tsunami hit the northeastern coast of Japan, and nuclear plants were damaged to a large extent. Before the Tsunami, 54 nuclear plants were operated, however presently, only three nuclear plants are in operation. Therefore, Japan is suffering from high price of electricity and low supply reliability. In generation mix, nuclear plants generate only one percent and 87 percent of electricity is generated by fossil energy. This is not preferable for Japan from the viewpoints of energy security and stable electricity supply. Therefore, it is mandatory to increase sustainable energy and to decrease consumption of fossil fuel. International grid connection and the Global Energy Interconnection will be one of countermeasures against these problems existing in Japan. In this paper, international grid connection initiatives proposed in Asia are described and features and issues of electric power grids in Japan are discussed to implement the international grid connection. As the key technology for implementing the international grid connection, the present status of DC superconducting power transmission lines and power converters for long-distance power transmission lines are presented. In conclusion, conditions and legal frameworks for realizing the international grid connection are described.

关键词： International grid connection Global Energy Interconnection Sustainable energy DC superconducting Power converter

Square-Scheme Electrochemistry in Battery Electrodes

在线全文

学校读者我要写书评

暂无评论

Accounts of Materials Research 2022年第1期3卷 33-41页

作者： masashi Okubo Kosuke Kawai Zihan Ma Atsuo Yamad Department of Electrical Engineering and Bioscience School of Advanced Science and EngineeringWaseda UniversityTokyo 169-8555Japan Department of Chemical System Engineering School of EngineeringThe University of TokyoTokyo 113-8656Japan

CONSPECTUS:Sustainable development cannot be achieved without substantial technological *** instance,flexible electricity management requires smart power sourcing with advanced energy storage/conversion *** for abrupt power spikes/drops observed in renewable energy sources such as solar and wind require rapid load-leveling using high-power energy storage systems when they are integrated into a *** energy storage devices efficiently convert electrical and chemical energy,which can potentially function as distributed power *** these,lithium-ion batteries are a present de facto standard with their relatively high energy density and energy efficiencies that are based on topochemical intercalation chemistry,whereby guest lithium ions are(de)intercalated reversibly with simultaneous redox reactions and minimal structural ***,their energy density,power density,life-cycle cost,calendar life,and safety remain unsatisfactory for widespread *** the storage capacity is maximized,as a result of which a labile deep charge/discharge state is generated,to develop batteries with high energy density,subsequent irreversible phase transformations or chemical reactions occur in many *** combination of the reversible electrode reactions and the subsequent irreversible phase transformations sometimes causes a charge/discharge curve characterized by a large voltage hysteresis with 100%Coulombic *** a large voltage hysteresis significantly degrades the energy efficiency,unveiling the reaction mechanism is of primary importance in mitigating energy loss.

关键词： Battery lithium irreversible

Adhesion Force Measurements of Polymer Particles by Detachment Field Method

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

仪器仪表学报 2004年第S3期 62-66页

作者： masashi Nagayama ,Nobuyasu Sakurai,Tatsuaki Wada,Manabu Takeuchi Department of Electrical and Electronic Engineering Ibaraki University Hitachi 316-8511 Japan Department of Electrical and Electronic Engineering Ibaraki University Hitachi 316-8511 Japan Department of Electrical and Electronic Engineering Ibaraki University Hitachi 316-8511 Japan Department of Electrical and Electronic Engineering Ibaraki University Hitachi 316-8511 Japan

The adhesion force distributions of polymer particles to aluminum substrates were measured by the detachment field method. Polymer particles with conducting surface treatment were used for the measurements. Further the conventional detachment field method was modified to be applicable to the adhesion force measurements of a single particle. The adhesion force of the polymer particles increased with an increase in relative humidity. The surface roughness of the substrate influenced the adhesion forces of particles significantly. The influence of the CF4 plasma treatment of the polymer particles and thin layer coating of the substrate surface on the adhesion forces of the polymer particles was also studied, and factors affecting adhesion forces of polymer particles are discussed.

关键词： Toner Adhesion force van der Waals force

Expression of the tumor suppressor gene maspin and its significance in intraductal papillary mucinous neoplasms of the pancreas

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 86-90页

作者： Kyoko Kashima Nobuyuki Ohike Seishiro Mukai masashi Sato Manabu Takahashi Toshio Morohoshi Department of Pathology Showa University School of Medicine Department of Internal MedicineShowa University Toyosy Hospital Department of Pathology Showa University School of Medicine Department of Pathology Showa University School of Medicine Department of SurgeryShowa University School of Medicine Division of Pathology Ebara HospitalTokyo Japan

BACKGROUND: Maspin is a member of the serpin family of protease inhibitors and is thought to inhibit carcinoma invasion, metastasis, and angiogenesis and induce apoptosis. We examined maspin expression immunohistochemically and assessed its significance in intraductal papillary mucinous neoplasm (IPMN) of the pancreas. METHODS: We examined 39 surgically resected specimens of IPMN that included 17 adenomas (IPMAs), 5 borderline tumors (IPMBs), 4 non-invasive carcinomas (non-invasive IPMCs), and 13 invasive carcinomas (invasive IPMCs). Immunostaining was performed according to the EnVision ChemMate method. The degree of maspin expression was scored and assessed according to the percentage and staining intensity of positive cells. RESULTS: Maspin expression was minimal in normal pancreatic duct epithelium, whereas in IPMNs, maspin was expressed in neoplasms of all stages. Maspin expression increased with increasing grade from IPMAs, IPMBs, to non-invasive IPMCs but decreased significantly in invasive IPMCs. No specific association between maspin expression and mucin type was found. Analysis of maspin expression with respect to clinicopathologic factors in cases of invasive IPMC revealed a greater extent of invasion in cases of low maspin expression and significantly fewer apoptotic cells in the ***: Maspin was expressed at high levels in IPMNs at various stages from adenoma to invasive carcinoma, and our results suggest that maspin may be involved in the occurrence and progression of IPMN. In addition, our data suggest that the apoptosis-inducing action of maspin suppresses invasion and progression of IPMN.

关键词： maspin intraductal papillary mucinous neoplasm pancreas apoptosis immunohistochemistry