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Hyperbaric Oxygen Stimulates the Proliferation and Differentiation of Raw264.7 Cells

Open Journal of Stomatology 2015年第12期5卷 309-320页

作者： Tomotaka Sugi masanori sasaki Takahiro Yamashiro Takaoki Tsuneyoshi Arioka Masaki Akihiko Matsumoto Kumamaru Wataru Nakano Hiroyuki Mori Yoshihide Section of Oral and Maxillofacial Surgery Division of Maxillofacial Diagnostic and Surgical Sciences Faculty of Dental Science Kyushu University Japan

Background: Hyperbaric Oxygen (HBO) Therapy improves the outcome of various types of sugery, such as postoperative bone grafts, fixation of jaw fractures, and osteoplasty of jaw deformities. Therefore, it is important to determine the effects of HBO on bone regeneration. The purpose of this study was to clarify the influence of a hyperbaric oxygen environment on bone regeneration at the osteoclastogenic cytokines level. Sample & Methods: RAW264.7 cells were stimulated under the various conditions by using a hyperbaric oxygen chamber. We evaluated the ability of the RAW264.7 macrophages to proliferate, differentiate and produce various osteoclastogenic cyto-kines. Results: A hyperbaric oxygen (HBO) environment and high concentration oxygen (HCO) environment increase cellular proliferation in a time-dependent manner. On the other hand, a HCO environment and a hyperbaric with room air (HBA) environment enhanced the differentiation of RAW264.7 cells. In addition, NFATc1 and c-Fos were increased by both the HBA environment and HCO environment. However, the effects of HBA and HCO environments were in contrast with each other with regard to RANK, TNF-α, C-FMS and TRAP. Conclusions: It was suggested that a HBO environment influenced the bone regeneration by altering osteoclastogenic cytokines level, and that a HCO environment and HBA environment acted independently on the proliferation and differentiation of macrophages and the secretion of osteoclastogenic cytokines, and that they acted in concert in a hyperbaric oxygen environment to induce conditions favoring regeneration.

关键词： Hyperbaric Oxygen Therapy Bone Regeneration Osteoclast RAW264.7

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Overexpression of β3/γ2 chains of laminin-5 and MMP7 in biliary cancer

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World Journal of Gastroenterology 2009年第31期15卷 3865-3873页

作者： Toshikuni Oka Hiroyuki Yamamoto Shigeru sasaki masanori Ii Keiichi Hizaki Hiroaki Taniguchi Yasushi Adachi Kohzoh Imai Yasuhisa Shinomura First Department of Internal Medicine Sapporo Medical University School of Medicine Sapporo Medical University

AIM:To clarify the clinicopathological significance of laminin-5 γ2 (LNγ2) and β3 (LNβ3) chains and MMP7 expression in biliary tract ***: We analyzed the association between immunohistochemically detected LNγ2, LNβ3, and MMP7 expression in biliary tract cancer and clinicopathological characteristics. Activity of MMP7 was analyzed by casein zymography. An in vitro invasion assay after treatment with MMP7-specific siRNA was ***: LNγ2 expression was predominantly observed in carcinoma cells at the invasive front. LNγ2 expression was seen in 57% of patients with biliary tract cancer, and was associated with depth of invasion, histologic type, and advanced stage. The expression pattern of LNβ3 was classified into two types: invasive front dominant type (38%) and diffuse type (28%).The invasive front dominant type was associated with histologic type and advanced stage. MMP7 positivity was correlated with LNγ2 or LNβ3 expression but not with clinicopathological characteristics. Active MMP7 detected by casein zymography was correlated with depth of invasion and advanced stage. Downregulation of MMP7 expression by siRNA resulted in a significant decrease in biliary tract cancer cell invasion in ***: Our results suggest that LNγ2 and LNβ3, in conjunction with MMP7, play a key role in the progression of biliary tract cancer.

关键词：胆道粘蛋白癌 siRNA 临床病理病理特征肿瘤细胞化学检测

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Remain Aggressive Behavior in Children with Temporal Lobe Epilepsy after Surgery

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Journal of Behavioral and Brain Science 2015年第3期5卷 81-87页

作者： Sayuri Sukigara Eiji Nakagawa masanori Ishikawa Kenji Sugai Masayuki sasaki Takanobu Kaido Akio Takahashi Taisuke Otsuki Department of Child Neurology National Center Hospital National Center of Neurology and Psychiatry (NCNP) Tokyo Japan Department of Psychiatry NCNP Tokyo Japan Department of Neurosurgery NCNP Tokyo Japan

Behavioral problems have a high rate in patients with temporal lobe epilepsy but there have been few detailed reports about pediatric patients. We report two children with temporal lobe epilepsy who manifest behavior abnormality remaining after surgery. One child developed complex partial seizures at 10 years and 3 months of age caused by a left temporal ganglioglioma and manifested behavioral problems of lability, aggression, impulsivity and disinhibition. Seizures were relieved after temporal lobe resection at 10 years and 8 months of age but behavioral symptoms had been remained. The second child developed epilepsy at 3 years of age. He manifested similar behavioral problems at 7 years old. Seizures were relieved after temporal lobe resection at 13 years old but behavioral symptoms had been remained. Their courses suggested that aggressive behavior might be associated with not only temporal lesion but also other lesions such as frontal lobe or neural circuitry between both lobes. It is important to inform families of patients that behavioral disorders may not improve after epileptic surgery.

关键词： Temporal Lobe Epilepsy Behavioral Disorder Aggression Epileptic Surgery

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Granulocyte Colony-Stimulating Factor Enhances the Anticancer Effects of Cisplatin against Lung Cancer by Promoting Angiogenesis

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Advances in Lung Cancer 2017年第1期6卷 1-11页

作者： Yun-Mo Li Yasushi Ohno Norihiko Funaguchi Takenobu Gomyo Yuka sasaki Sayaka Toyoshi Daizo Kaito Komei Yanase Junki Endo Fumitaka Ito masanori Kawasaki Shinya Minatoguchi Department of Cardiology and Respirology Graduate School of Medicine Gifu University Gifu Japan Department of Respiratory Medicine Murakami Memorial Hospital Asahi University Mizuho Japan

The G-CSF is used as a therapeutic drug of the febrile neutropenia in lung cancer chemotherapy, however, there were few reports that showed the effects of combination effects of G-CSF and anticancer drugs against lung cancer. In the present study, we investigated the effects of G-CSF and the combination effects of G-CSF and cisplatin on lung cancer growth. We investigated the effect of G-CSF against the LL-2 and KLN-205 cells by MTT assay and tried to detect the G-CSF receptor by RT-PCR. Next, to analyze the G-CSF effects in vivo, we transplanted the LL-2 into C57BL/6 mice, intraperitoneally administered G-CSF (30 micro/kg/day) with or without cisplatin (5 mg/kg), measured the tumor size and analyzed pathologically by HE and immunostaining. In vitro analyses, G-CSF showed no effects in LL-2 and KLN-205 cells, and RT-PCR revealed no G-CSF receptor mRNA. In vivo analyses, G-CSF alone did not significantly suppress tumor growth. However, concurrent G-CSF administration with cisplatin significantly enhanced the tumor suppressing effect of cisplatin in early stage of tumor growth. The analysis data of vWF immunostaining indicated that the neovascularization in the peripheral region of the tumors was more enhanced in G-CSF treatment mice. ELISA assay revealed that G-CSF did not influence the serum concentration of TNF-alpha and IL-12 in tumor-bearing mice. This study suggests that concurrent (combination) administration of cisplatin with G-CSF is a safe and effective method for enhancing anticancer effects and reducing chemotherapeutic agent-induced myelosuppression.

关键词： Granulocyte Colony-Stimulating Factor Lung Cancer G-CSF Receptor Angiogenesis Anticancer Agent Cisplatin TNF-Alpha IL-12

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Measurement of Viscosity Alteration for Emulsion and Numerical Simulation on Bitumen Production by SAGD Considering In-situ Emulsification

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Journal of Earth Science and Engineering 2016年第1期6卷 10-17页

作者： Junpei Kumasaka Kyuro sasaki Yuichi Sugail Olalekan S. Alade masanori Nakano Department of Earth Resources Engineering Kyushu University Fukuoka 819-0395 Japan Research Center Japan Petroleum Exploration Co. Ltd. Chiba 261-0025 Japan

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