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VEGF、ANG-1、ANG-2、TSP-1的表达与胆管细胞性肝癌血管生成和侵润转移的关系

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中国病理生理杂志 2007年第10期23卷 2046-2050页

作者：汤地周奇梁力建彭宝岗永野皓昭中山大学附属第一医院肝胆外科广东广州510080 中山大学附属第一医院黄埔院区广东广州510700 大阪大学附属病院消化器外科

目的:研究血管内皮生长因子(VEGF)、血管生成素-1(ANG-1)、血管生成素-2(ANG-2)、血小板反应蛋白-1(TSP-1)的表达与胆管细胞性肝癌(CCC)血管生成和侵润转移的关系。方法:对33例手术切除的CCC标本进行CD34、VEGF、ANG-1、ANG-2和TSP-1的... 详细信息

目的:研究血管内皮生长因子(VEGF)、血管生成素-1(ANG-1)、血管生成素-2(ANG-2)、血小板反应蛋白-1(TSP-1)的表达与胆管细胞性肝癌(CCC)血管生成和侵润转移的关系。方法:对33例手术切除的CCC标本进行CD34、VEGF、ANG-1、ANG-2和TSP-1的免疫组化染色,研究VEGF、ANG-1、ANG-2、TSP-1的表达与胆管细胞性肝癌血管生成和肿瘤门静脉侵犯、肝内转移、淋巴结转移以及肿瘤分化水平之间的关系。结果:本组CCC的微血管密度(MVD)为(87.2±52.6)/mm2,VEGF、ANG-1、ANG-2和TSP-1的阳性率分别为75.6%、36.0%、57.6%和45.5%。VEGF和ANG-2的阳性表达与高MVD相关,TSP-1则与MVD负相关(P<0.01,P<0.05,P<0.01)。阳性TSP-1与肝内转移正相关(46.7%vs5.6%,P<0.05)。结论:CCC瘤内的血管新生活跃,VEGF和ANG-2的阳性表达与CCC血管生成正相关,TSP-1则与其负相关,TSP-1的阳性表达还与肝内转移相关,VEGF、ANG-1、ANG-2的表达与肿瘤的侵润转移未见显著相关。

关键词：肝肿瘤血管内皮生长因子血管生成素类血小板反应蛋白1 肿瘤侵润肿瘤转移

胆管细胞性肝癌的血管新生及临床病理意义(英文)

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中国现代医学杂志 2005年第11期15卷 1601-1608,1612页

作者：汤地梁力建黄洁夫永野皓昭广州中山大学附属第一医院肝胆外科广东广州510080 大阪大学附属病院消化器外科

目的目前关于胆管细胞性肝癌(CCC)的血管新生研究较少,本研究的目的在于描述CCC的血管新生的特点,其与血管新生调控因子之间的关系以及临床- 病理意义。方法调查33例手术切除的CCC标本。肿瘤微血管密度(MVD )使用抗CD34 抗体进行标记,... 详细信息

目的目前关于胆管细胞性肝癌(CCC)的血管新生研究较少,本研究的目的在于描述CCC的血管新生的特点,其与血管新生调控因子之间的关系以及临床- 病理意义。方法调查33例手术切除的CCC标本。肿瘤微血管密度(MVD )使用抗CD34 抗体进行标记,并进行了VEGF Ang-1 Ang-2 and TSP-1的免疫组化染色。结果本组C CC的MVD为(87.2±52.6),范围为10-229。VEGF Ang-1 Ang-2和TSP-1的阳性率分别为75.6% ,36% ,57.6%和45.5% 。VEGF和Ang-2的阳性表达与高MVD相关,TSP-1则负相关(P值分别为0.004,0.015和0.005)。阳性TSP-1 与肝内转移正相关(46.7% vs.5.6% ,P=0.012)。除此之外,并没有发现VEGF Ang-1 Ang-2和TSP-1的阳性表达与肿瘤大小,包膜形成,包膜浸润,门脉侵犯,肝内转移,细胞分化之间的相关性。MVD的高低以及VEGF,Ang-1,Ang-2和TSP-1的阳性表达与根治性切除的23例的术后生存率之间没有发现相关性。结论CCC瘤内的血管新生活跃,其瘤内血管新生与病理类型有一定的关系。VEGF和Ang2的阳性表达与CCC血管新生正相关,TSP-1则与其负相关。TSP-1的阳性表达还与肝内转移正相关,除此此外,并没有发现MVD和VEGF,ANG1,orANG2的表达与肿瘤的恶性程度或者预后相关。

关键词：胆管细胞性肝癌 VEGF angiopoietin thronlbospodin 血管新生预后

表达不同氨基端二级结构HCVNS3/4A点突变质粒的构建及在Huh7细胞中的表达

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南方医科大学学报 2009年第4期29卷 720-723,731页

作者：王雪萍李富军长野(藤井)基子北山喜久美堀田博第四军医大学学报编辑部陕西西安710033 兰州军区兰州总医院医务部甘肃兰州730050 神户大学大学院医学系研究科微生物学分野

目的构建表达不同氨基端二级结构HCVNS3/4A的点突变质粒,并在Huh7细胞中进行表达。方法以pSG5/M-H05-5/4A为模板(A1-1),根据分型标准设计点突变引物。首先构建4个单点突变质粒:pSG5/M-H05-5(A1-2)/4A(A1-2)(Y56F),pSG5/M-H05-5(B1-1)/4A(B1-1)(L80Q),pSG5/M-H05-5(B2-1)/4A(B2-1)(V51A),pSG5/M-H05-5(B2-2)/4A(B2-2)(S61A)。然后,分别以A1-2,B2-1,B2-2为模板,再将第80位的赖氨酸点突变为谷氨酸(L80Q),构建另外3个双点突变质粒:pSG5/M-H05-5(B1-2)/4A(B1-2),pSG5/M-H05-5(A2-1)/4A(A2-1)和pSG5/M-H05-5(A2-2)/4A(A2-2)。每个质粒均进行序列测定验证点突变成功。用FuGene6转染试剂将构建物转染入Huh7细胞,并应用间接免疫荧光试验和Western blotting检测构建物的表达。结果免疫荧光实验检测到NS3的4种亚细胞定位方式:点状,弥漫样,面包圈样,及短棒状。Western Blotting亦显示构建物均成功表达,同时发现A2-1和B2-1亚型NS3/4A存在不完全切割现象,表明与其他亚型相比,A2-1和B2-1NS3 in cis丝氨酸蛋白酶活性较弱。结论成功构建表达不同氨基端二级结构HCV NS3/4A点突变质粒,为抗不同亚型HCV的深入研究提供基础。

关键词： pSG5 点突变 HCVNS3/4A 二级结构 Huh 7细胞

Oscillation frequency stabilization of a diode laser for the laser interferometer in a satellite-to-satellite tracking system

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pSG5/NS5A5BΔC质粒的构建及在Huh7细胞中的表达

Photonics Asia 2004;Optics and Lasers

作者： S.Maehara H.Kobayashi T.Sato M.Ohkawa T.Maruyama T.Yoshino H.Kunimori M.Hosokowa H.Ito Y.Li S.nagano S.Kawamura C/O Sato Lab. Graduate School of Science and Technology Niigata University Ikarashi 2-no-cho Niigata 950-2181 JAPAN C/O Sato Lab. Graduate School of Science and Technology Niigata University Ikarashi 2-no-cho Niigata 950-2181 JAPAN Faculty of Engineering Niigata University Ikarashi 2-no-cho Niigata 950-2181 JAPAN Faculty of Engineering Niigata University Ikarashi 2-no-cho Niigata 950-2181 JAPAN Faculty of Engineering Niigata University Ikarashi 2-no-cho Niigata 950-2181 JAPAN National Institute of Information and Communications Technology 4-2-1 Nukui-Kitamachi Koganei Tokyo 184-8795 Japan National Institute of Information and Communications Technology 4-2-1 Nukui-Kitamachi Koganei Tokyo 184-8795 Japan National Institute of Information and Communications Technology 4-2-1 Nukui-Kitamachi Koganei Tokyo 184-8795 Japan National Institute of Information and Communications Technology 4-2-1 Nukui-Kitamachi Koganei Tokyo 184-8795 Japan National Institute of Information and Communications Technology 4-2-1 Nukui-Kitamachi Koganei Tokyo 184-8795 Japan National Institute of Information and Communications Technology 4-2-1 Nukui-Kitamachi Koganei Tokyo 184-8795 Japan National Astronomical Observatory 2-21-1 Osawa Mitaka Tokyo 181-8588 Japan

Currently in the initial stages of development, the endeavor aims to use satellite-to-satellite tracking laser interferometer-based optical technique, to document fluctuations in earth’s gravitationa

关键词： Earth’s gravitational field, satellite, interferometer, diode laser, frequency stability, femtosecond optical comb generator, rubidium absorption line, optical feedback

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细胞与分子免疫学杂志 2009年第1期25卷 31-34页

作者：王雪萍李富军邓琳长野(藤井)基子北山喜久美堀田博第四军医大学学报编辑部陕西西安710032 兰州军区兰州总医院医务部甘肃兰州730050 神户大学大学院医学系研究科

目的:构建pSG5/NS5A5B△C质粒,并检验其在Huh7细胞中的瞬时表达。方法:使用已经构建的pTM1-NS2NS5A5B△C质粒为模板,根据pTM1、HCV NS5A5B△C、pSG5序列和酶切位点特点设计引物,PCR扩增得到HCVNS5A5B△C基因片段,将目的片段插入pSG5载体... 详细信息

目的:构建pSG5/NS5A5B△C质粒,并检验其在Huh7细胞中的瞬时表达。方法:使用已经构建的pTM1-NS2NS5A5B△C质粒为模板,根据pTM1、HCV NS5A5B△C、pSG5序列和酶切位点特点设计引物,PCR扩增得到HCVNS5A5B△C基因片段,将目的片段插入pSG5载体中,经筛选阳性克隆、SacⅠ和BglⅡ分别酶切鉴定后,用FuGene6转染试剂转染入Huh7细胞。用免疫荧光染色、Western blot检测HCV NS5A5B△C在Huh-7细胞中的表达。结果:限制性内切酶SacⅠ和BglⅡ分别酶切鉴定后,琼脂糖凝胶电泳结果显示酶切片段大小和插入方向均与预计一致。荧光显微镜下可见转染的Huh7细胞表达带有绿色荧光的HCV NS5A5B△C蛋白,该蛋白主要表达于细胞质,表达率达40%以上。West-ern blot结果也证实在转染pSG5/NS5A5B△C的Huh7细胞中出现了大小与HCV NS5A5B△C(82ku)一致的条带。结论:成功构建了pSG5/NS5A5B△C质粒,并在Huh7细胞中成功瞬时表达,为进一步研究HCV蛋白的功能奠定了基础。

关键词：丙型肝炎病毒非结构蛋白5A5B pSG5 Huh7细胞

Screening and Phylogenetic Analysis of Deep-Sea Bacteria Capable of Metabolizing Lignin-Derived Aromatic Compounds

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Open Journal of Marine Science 2012年第4期2卷 177-187页

作者： Y. Ohta S. Nishi T. Haga T. Tsubouchi R. Hasegawa M. Konishi Y. nagano Y. Tsuruwaka Y. Shimane K. Mori K. Usui E. Suda K. Tsutsui A. Nishimoto Y. Fujiwara T. Maruyama Y. Hatada Institute of Biogeosciences Japan Agency of Marine-Earth Science and Technology (JAMSTEC) Yokosuka Japan

Lignin is one of the most abundant biomasses in nature. It is composed of aromatic moieties and has great potential for use in the production of chemical alternatives to petroleum products. Because of increasing interest in biocatalysis, the potential for industrial application of microbial metabolism of lignin-derived compounds has gained considerable recent attention. Functional screenings of culturable bacteria isolated from sediments and sunken wood collected from the deep sea revealed the existence of a number of previously unidentified bacteria capable of metabolizing lignin-related aromatic compounds. Of the 510 isolates obtained in the present study, 208 completely or partially metabolized these compounds. The 208 isolates were classified into diverse phyla, including Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria. Among the 208 isolates, 61 unique 16S rRNA gene sequences were detected including previously unidentified marine lineage isolates. The metabolites of the isolates were analysed using liquid chromatography/mass spectrometry (LC/MS) or gas chromatography/mass spectrometry (GC/MS). Most of the representative 61 isolates non-oxidatively decarboxylated the substrates to produce the corresponding aromatic vinyl monomers, which are used as feed stocks for bio-based plastics production. Oxidative metabolism of the lignin-related compounds for assimilation was frequently observed. Our study showed that the deep-sea environment contains an abundance of microorganisms capable of both non-oxidative and oxidative bioconversion of lignin-derived aromatic compounds. The ability for bio-conversion of aromatic compounds found in this study will facilitate the development of future biotechnological applications.

关键词： Aromatic Compound Bacteria Deep Sea Lignin

The thymidylate kinase gene homologues identified from various jujube witches'-broom phytoplasma isolates infecting different jujube cultivars in China

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The thymidylate kinase gene homologues identified from vario...

雷莫芦单抗用于经乐伐替尼治疗后进展的不可切除肝细胞癌的治疗效果

第三届中国森林保护学术大会

作者： Qicong Xu~(1,2) Guozhong Tian~(1**) Caili Lin~1 Yong Li~1 Chungen Piao~1 1 Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China 2 International Nature Farming Research Center,5632 Hata,nagano 390-1401,Japan

Thymidylate kinase(TMK,EC 2.***.4.9) that catalyses the transfer of a terminal phosphoryl group from ATP to dTMP,is crucial to both the de novo synthetic and the salvage pathways for pyrimidine *** types of thymidylate kinase genes,tmkX,tmkY and tmkZ(TMK gene),of the phytoplasma associated with jujube witches’-broom disease(JWB) were identified by means of sequence analyses as well as RFLP and SSCP of the specific PCR products in thirty four JWB infected jujube samples collected from different cultivars with diverse geographic origins and resistance to the JWB in *** and tmkY gene sequences were identified in many jujube cultivars from different regions, whereas tmkZ was only found in BJLZ strain infecting an aged dragon jujube from Beijing,which contained tmkX sequence as *** and tmkY have the closest relationship with tmk-a gene (AB010446) in OY-W strain of onion yellows and tmkZ have the closest relationship with one of tmk gene copies in genome of OY-M(AP006628,from 391588 to 392202).Amino acid sequence analyses showed that all the three deduced proteins TMKx,TMKy and TMKz of tmkX,tmkY and tmkZ contained conserved motifs for catalytic *** collected JWB isolates inhabiting different resistanct cultivars against the phytoplasma could be mainly classified into three types,typeX,typeY and typeXY,containing tmkX gene, tmkY gene and both tmkX and tmkY genes,*** portion of typeXY strains were detected than typeX and Y from the collection of JWB *** with mere typeX or typeY sequeces seem to be frequenctly in association with susceptible cultivars,while the typeXY strain was likely to be much related to resistant *** is assumed that the formation and distribution of different tmk genotype clusters among JWB strain population might be related to the phytoplasmal gene duplication and the selection by the infected cultivars and geographical environment as well as the gene recombination due to

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Gastroenterology Report 2021年第2期9卷 133-138,I0002页

作者： Atsushi Hiraoka Takashi Kumada Toshifumi Tada Chikara Ogawa Joji Tani Shinya Fukunishi Masanori Atsukawa Masashi Hirooka Kunihiko Tsuji Toru Ishikawa Koichi Takaguchi Kazuya Kariyama Ei Itobayashi Kazuto Tajiri Noritomo Shimada Hiroshi Shibata Hironori Ochi Kazuhito Kawata Hidenori Toyoda Hideko Ohama Kazuhiro Nouso Akemi Tsutsui Takuya nagano Norio Itokawa Korenobu Hayama Taeang Arai Michitaka Imai Yohei Koizumi Shinichiro Nakamura Kojiro Michitaka Yoichi Hiasa Masatoshi Kudo Gastroenterology Center Ehime Prefectural Central HospitalEhimeJapan Department of Gastroenterology and Hepatology Ogaki Municipal HospitalGifuJapan Department of Internal Medicine Himeji Red Cross HospitalHyogoJapan Department of Gastroenterology and Hepatology Takamatsu Red Cross HospitalTakamatsuJapan Department of Gastroenterology and Hepatology Kagawa universityKagawaJapan Department of Gastroenterology Osaka Medical CollegeOsakaJapan Division of Gastroenterology and Hepatology Department of Internal MedicineNippon Medical SchoolTokyoJapan Department of Gastroenterology and Metabology Ehime University Graduate School of MedicineEhimeJapan Center of Gastroenterology Teine Keijinkai HospitalSapporoJapan Department of Gastroenterology Saiseikai Niigata HospitalNiigataJapan Department of Hepatology Kagawa Prefectural Central HospitalTakamatsuJapan Department of Gastroenterology Okayama City HospitalOkayamaJapan Department of Gastroenterology Asahi General HospitalAsahiJapan Department of Gastroenterology Toyama University HospitalToyamaJapan Division of Gastroenterology and Hepatology Otakanomori HospitalKashiwaJapan Department of Gastroenterology Tokushima Prefectural Central HospitalTokushimaJapan Hepato-biliary Center Matsuyama Red Cross HospitalMatsuyamaJapan Department of Hepatology Hamamatsu University School of MedicineHamamatsuJapan Department of Gastroenterology Kindai UniversityOsakaJapan

背景:在日本,乐伐替尼可用于不可切除原发性肝癌的一线、二线或三线治疗。本研究评估新近研发的雷莫芦单抗用于经乐伐替尼治疗后进展的不可切除的原发性肝癌的疗效。方法:2018年5月至2020年1月间在日本16家中心接受乐伐替尼治疗的385例... 详细信息

背景:在日本,乐伐替尼可用于不可切除原发性肝癌的一线、二线或三线治疗。本研究评估新近研发的雷莫芦单抗用于经乐伐替尼治疗后进展的不可切除的原发性肝癌的疗效。方法:2018年5月至2020年1月间在日本16家中心接受乐伐替尼治疗的385例不可切除原发性肝癌患者中,我们将其中接受雷莫芦单抗作为下一线治疗的28例患者纳入研究,对其治疗效果进行回顾性分析。结果:28例患者中位年龄70岁,中位白蛋白-胆红素(ALBI)评分为2.19。28例患者中,23例为男性;21例为Child-Pugh A级,7例为Child-Pugh B级;3例为BCLC B期,25例为BCLC C期。雷莫芦单抗用于二线、三线和四线治疗的病例分别为14例、9例和5例患者。26例患者获得疗效评估,客观缓解率为3.8%(1/26),疾病控制率为42.3%(11/26);中位疾病进展时间为2个月。未继续接受雷莫芦单抗治疗的原因包括16例出现疾病进展和2例出现3级不良反应(消化道出血和腹水)。结论:我们的初步研究显示,对于乐伐替尼治疗进展的不可切除原发性肝癌,雷莫芦单抗并没有获得预期的治疗效果。

关键词： hepatocellular carcinoma ramucirumab lenvatinib sorafenib

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大气492GHz亚毫米波不透明度的测量

天文学报 2004年第1期45卷 79-87页

作者：杨戟孙成华黄树频大石雅寿宫泽敬辅中国科学院紫金山天文台南京210008 日本国立天文台野边山宇宙电波观测所

使用移动式亚毫米波望远镜(POST)在位于青藏高原海拔3200米的紫金山天文台德令哈射电天文观测站址测量地球大气492GHz频率处天顶方向的不透明度((?)0)的结果.在1999-2000年冬季和2000-2001年冬季的两个观测季节内累计共进行了约870个小... 详细信息

使用移动式亚毫米波望远镜(POST)在位于青藏高原海拔3200米的紫金山天文台德令哈射电天文观测站址测量地球大气492GHz频率处天顶方向的不透明度((?)0)的结果.在1999-2000年冬季和2000-2001年冬季的两个观测季节内累计共进行了约870个小时的测量,取得了25842组(?)0的有效数据.对数据的统计表明,观测季节内(?)0值主要分布在1.5-3.0之间.观测时段内大气不透明度(?)0<1.0的时间比例约占3%.在给出实测资料的基础上,将所测量的亚毫米波不透明度与国际现有亚毫米波台址的不透明度进行了初步比较.

关键词：测量亚毫米波大气不透明度选址