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Anti-Inflammatory and Immunostimulatory Effects of Extract from Culture of Effective Microorganisms (ECEM) Revealed by Functional Genomics and Metabolome Analyses

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Food and Nutrition Sciences 2015年第12期6卷 1115-1125页

作者： masaki shintani Fusako Mitsunaga Shun Shimabukuro Shin Nakamura EM Research Organization Inc. Kitanakagusuku Japan Intelligence and Technology Lab Inc. Kaizu Japan Biomedical Institute NPO Primate Agora Inuyama Japan

The extract from culture of effective microorganisms (ECEM) is a secondary metabolite produced in symbiotic culture of a mixture of yeasts and lactic bacteria followed by photosynthetic bacteria. Metabolomics studies have demonstrated that ECEM contained several active ingredients associated with anti-inflammation through interactions with nuclear factor-κB. Functional genomics using DNA microarrays revealed that ECEM had distinctive anti-inflammatory and immunostimulatory actions in RAW264.7 cells. ECEM inhibited the LPS-upregulated expression of one group of pro-inflammatory genes (iNos, Il6 and Cox2) but not another group genes (Tnf, Ccl7 and Dusp2);it also upregulated the expression of Cd1d1, which participates in antigen presentation on lipids, resulting in activation of natural killer T (NKT) cells related to infection defense and cancer. Bacterial glycosylated lipid and other related complex lipids in ECEM are thought to trigger this upregulation of Cd1d1 expression. These results suggest that ECEM functions in the immune response in both inflammation and antigen presentation and that it contains components important for these functions. These novel effects of ECEM could be invaluable for developing functional drinks or health beverages.

关键词： DNA Microarray Secondary Metabolites iNos Il6 Cd1d1

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Harnessing a T1 Phage-Derived Spanin for Developing Phage-Based Antimicrobial Development

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BioDesign Research 2024年第1期6卷 28-38页

作者： Wakana Yamashita Shinjiro Ojima Azumi Tamura Aa Haeruman Azam Kohei Kondo Zhang Yuancheng Longzhu Cui masaki shintani Masato Suzuki Yoshimasa Takahashi Koichi Watashi Satoshi Tsuneda Kotaro Kiga Research Center for Drug and Vaccine Development National Institute of Infectious DiseasesTokyo 162-8640Japan Department of Life Science and Medical Bioscience Waseda University2-2 Wakamatsu-choShinjuku-kuTokyo 162-8480Japan Division of Infectious Diseases Advanced Clinical Research CenterThe Institute of Medical ScienceThe University of TokyoTokyo 108-8639Japan Antimicrobial Resistance Research Center National Institute of Infectious DiseasesTokyoJapan Division of Bacteriology Department of Infection and ImmunitySchool of MedicineJichi Medical UniversityShimotsuke-shiTochigi 329-0498Japan Department of Engineering Graduate School of Integrated Science and TechnologyShizuoka University3-5-1 JohokuNaka-kuHamamatsuShizuoka432-8561Japan Phage Therapy Institute Comprehensive Research OrganizationWaseda University2-2 Wakamatsu-choShinjuku-kuTokyo 162-8480Japan

The global increase in the prevalence of drug-resistant bacteria has necessitated the development of alternative treatments that do not rely on conventional antimicrobial *** bacteriophage-derived lytic enzymes in antibacterial therapy shows promise;however,a thorough comparison and evaluation of their bactericidal efficacy are *** study aimed to compare and investigate the bactericidal activity and spectrum of such lytic enzymes,with the goal of harnessing them for antibacterial ***,we examined the bactericidal activity of spanins,endolysins,and holins derived from 2 Escherichia coli model phages,T1 and *** these,T1-spanin exhibited the highest bactericidal activity against ***,we expressed T1-spanin within bacterial cells and assessed its bactericidal activity.T1-spanin showed potent bactericidal activity against all clinical isolates tested,including bacterial strains of 111 ***,2 Acinetobacter spp.,3 Klebsiella spp.,and 3 Pseudomonas *** contrast,T1 phage-derived endolysin showed bactericidal activity against *** and ***,yet its efficacy against other bacteria was inferior to that of ***,we developed a phage-based technology to introduce the T1-spanin gene into target *** synthesized non-proliferative phage exhibited strong antibacterial activity against the targeted *** potent bactericidal activity exhibited by spanins,combined with the novel phage synthetic technology,holds promise for the development of innovative antimicrobial agents.

关键词： development antimicrobial developing harnessing phage-based phage-derived spanin

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