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Erythropoietin gene transfer into rat testes by in vivo electropo-ration may reduce the risk of germ cell loss caused by cryptorchidism

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Asian Journal of Andrology 2005年第4期7卷 369-373,页

作者： masaki Dobashi Kazumasa Goda Hiroki Maruyama Masato Fujisawa Division of Urology Department of Organs Therapeutics Faculty of Medicine Kobe University Graduate School of Medicine Kobe 650-0017 Japan Division of Nephrology and Rheumatology Niigata University Graduate School of Medical and Dental Sciences Niigata 951-8120 Japan

Aim： To investigate the effects of rat Erythropoietin （Epo） on spermatogenesis by transferring rat Epo gene into cryptorchid testes by means of in vivo electroporation. Methods： Sprague-Dawley rats with surgically-induced unilateral cryptorchidism were divided into three groups： the first group was given intratesticular injections of pCAGGS-Epo （pCAGGS-Epo group）, the second group was given intratesticular injections of pCAGGS （pCAGGS group）, and the third group were given intratesticular injections of phosphate-buffered saline （PBS group）. At the same time, square electric pulses of 30 V were applied six times with a time constant of 100 ms. One or two weeks after injection, each testis was weighed and the ratio of the total number of germ cells to that of Sertoli cells （G/S ratio） was calculated to evaluate the impairment of spermatogenesis. Ten testes taken from each of the three groups were examined at each time point. Results： The testicular weight after the injection of pCAGGS-Epo or pCAGGS control plasmid was （0.85 ± 0.08） g and （0.83 ± 0.03） g, respectively, at week 1 （P = 0.788） and （0.62 ± 0.06） g and （0.52 ± 0.02） g, respectively, at week 2 （P = 0.047）. At week 1, spermatids and sperm were more abundant in testes with pCAGGS-Epo than those in the control testes. At week 2, spermatids and sperm were hardly detected in either group. The G/S ratio was 23.27 ± 6.80 vs. 18.63 ± 5.30 at week 1 （P = 0.0078） and 7.16 ± 3.06 vs. 6.05 ± 1.58 at week 2 （P = 0.1471）, respectively. Conclusion： The transfer of Epo to rat testes by in vivo electroporation may reduce the risk of the germ cell loss caused by cryptorchidism.

关键词： electroporation gene transfer techniques erythropoietin spermatogenesis Epo

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Micro-scale Additive Manufacturing Using the Optical Potential Generated by a Bessel Beam

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Nanomanufacturing and Metrology 2020年第4期3卷 292-298页

作者： masaki Michihata Makoto Yokei Shotaro Kadoya Satoru Takahashi Department of Precision Engineering The University of Tokyo7-3-1 HongoBunkyoTokyo 113-8654Japan Research Center for Advanced Science and Technology The University of Tokyo4-6-1 KomabaMeguroTokyo 153-8904Japan

In this study,we proposed a novel micro-scale additive manufacturing method based on the optical potential formed by a Bessel *** proposed technique is expected to show no deterioration in manufacturing resolution due to heat genera-tion,to be applicable to various materials,and to be able to be performed in an air *** basic principle of the proposed method involves accumulating and stacking particles dispersed in air by using optical radiation *** this paper,the trajectory of the accumulated particles was numerically estimated and experimentally *** numerical and experimental results agreed well;specfially,the back ground flow carried the particles to the optical axis of the Bessel beam,and then the particles were localized at the bottom of the optical potential valley on the ***,a pillar structure was fabricated with polystyrene particles having a diameter of 1 um,which indicated that the proposed technique was promising for practical applications.

关键词： Additive manufacturing Optical radiation pressure Optical trapping potential Bessel beam Nanoparticles

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Design Study of Top Lid with Clamp Structure in JT-60SA Cryostat

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Plasma Science and Technology 2013年第2期15卷 188-191页

作者： Shigetoshi NAKAMURA Yusuke K. SHIBAMA Kei masaki Akira SAKASAI Japan Atomic Energy Agency Mukouyama 801-1 Naka Ibaraki 311-0193 Japan

JT-60SA is a fully superconducting coil tokamak upgraded from the JT-60U. This paper focused on the integrity of the top lid of cryostat in JT-60SA. The design requirement for the cryostat in normal operations is to achieve vacuum insulation of 10-1 Pa, and the top flange of the top lid is lightly welded onto its body flange. The weld is tensile-loaded by bending deformation of the top lid due to vacuum pressure of external 0.1 MPa. This weld integrity is evaluated with tensile-load reduction, which results in clamp reinforcement. The structural integrity of the top lid is validated.

关键词： clamp vacuum insulation top lid cryostat JT-60SA

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Enhancing mechanical behaviors of collapsible soil using two biopolymers

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Journal of Rock Mechanics and Geotechnical Engineering 2017年第2期9卷 329-339页

作者： Mohamed Ayeldeen Abdelazim Negm Mostafa El-Sawwaf masaki Kitazume Structural Engineering Department Tanta UniversityTantaEgypt Faculty of Engineering Zagazig UniversityZagazigEgypt Civil and Environmental Engineering Department Tokyo Institute of TechnologyTokyoJapan

This study aims to investigate the possibility of using biopolymer(environmental friendly material) to enhance the mechanical behaviors of collapsible *** types of biopolymers were(xanthan gum and guar gum) used in this study due to their stable behaviors under severe conditions and their availability with reasonable *** experimental program focused on three major soil properties,*** characterizations,collapsible potential and shear *** three properties are essential in process of soil *** biopolymer concentrations were used in this study and the experimental program was performed at two curing periods(soon after mixing the soil with the biopolymer and after one week curing time).Shear parameters were measured for the treated specimens under both soaked and unsoaked conditions,while a collapsible potential test was performed under different mixing conditions(wet mix and dry mix).A numerical model was built to predict the behavior of the treated collapsible soil after and before water *** results indicated that the ability of both xanthan gum and guar gum can be used as improvement materials for collapsible soil *** collapsible potential has been reduced from 9%to 1%after mixing the soil with 2%biopolymer concentration in the wet *** one week curing,the cohesion has been increased from 8.5 kPa to105 kPa by increasing the xanthan gum concentration from zero to 2%,leading to an overall improvement in soil shear *** also proves that the guar gum is superior to the xanthan *** shear strength of soil can be increased by about 30%when using the guar gum in comparison with the xanthan gum at the same conditions;however,the collapsible potential of soil material will be reduced by about 20%.

关键词： Collapsing soil Xanthan gum Guar gum Collapsible potential Shear strength Compaction characterizations Numerical model

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Autonomous Dual Active Power-frequency Control in Power System with Small-scale Photovoltaic Power Generation

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Journal of Modern Power Systems and Clean Energy 2022年第4期10卷 941-953页

作者： Noha Harag masaki Imanaka Muneaki Kurimoto Shigeyuki Sugimoto Hassan Bevrani Takeyoshi Kato Electrical Engineering Department of Nagoya University NagoyaJapan University of Kurdistan SanandajIran Institute of Materials and Systems for Sustainability of Nagoya University NagoyaJapan

Active power control of the photovoltaic(PV)power generation system is a promising solution to regulate frequency fluctuation in a power system with high penetration of renewable *** paper proposes an autonomous active power control of a small-scale PV system for supporting the inertial response of synchronous generators and power-frequency *** the proposed control approach,an effective grid frequency regulation scheme is realized using slow-and fast-frequency responses.A low-pass filter based frequency measurement is used for slow-frequency response,while direct frequency measurement is used for fast-frequency *** designed dual droop characteristic-based control is shaped to achieve a smooth transition between slow-and fast-frequency *** performance of the proposed control approach is demonstrated for serious disturbance scenarios,i.e.,considerable power-load imbalance and generation *** the powerload imbalance test scenario,the proposed control approach works properly within the normal frequency deviation region even when the frequency deviation exceeds that region *** the generation trip test,the frequency deviation is mitigated quickly,and the employed droop control is smoothly transferred from the slow-to fast-frequency responses.

关键词： Dual droop control fast-frequency response slow-frequency response inertial response power-frequency control regulation support

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An Efficient Framework to Utilize Grover Search

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南京邮电大学学报（自然科学版） 2011年第2期31卷 49-58页

作者： Shigeru Yamashita masaki Nakanishi College of Information Science and Engineering Ritsumeikan University

This paper proposes an efficient framework to utilize quantum search *** the best of our knowledge,this is the first paper to show a concrete usage of quantum search in general *** our framework,we can utilize a quantum computer as a coprocessor to speed-up some parts of a program that runs on a classical *** do so,we propose several new ideas and techniques,such as a practical method to design a large quantum circuits for search problems and an efficient quantum comparator.

关键词： quantum search general programming cooperation of quantum and classical computers quantum comparator quantum circuit design

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Is sarcopenic obesity superior to sarcopenia as a predicting indicator in patients with hepatocellular carcinoma following hepatic resection?

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Hepatobiliary Surgery and Nutrition 2020年第2期9卷 202-204页

作者： Shinji Itoh Tomoharu Yoshizumi masaki Mori Department of Surgery and Science Graduate School of Medical SciencesKyushu UniversityFukuokaJapan

The most common primary cancer derived from the liver is hepatocellular carcinoma(HCC),and its incidence is increasing globally(1).Hepatic resection has been established as a safe and effective treatment in patients with ***,the number of patients who develop recurrence after hepatic resection remains high(2).The body composition,which consists of the skeletal muscle mass and fat area,is an important used to determine the perioperative risk and prognosis in patients with cancer.

关键词： patients resection hepatic

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Study of Electron Orbits for Formation of Toroidal Closed Flux Surface by ECH

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Plasma Science and Technology 2011年第3期13卷 342-346页

作者： Tak,ushi MAEKAWA Tomokazu YOSHINAGA Hitoshi TANAKA masaki UCHIDA Fumitake WATANABE Graduate School of Energy Science Kyoto University Kyoto 606-8502 Japan National Institute for Fusion Science Toki 509-5292 Japan

Electron orbits under the external vertical field （Bv） and the self poloidal field of the toroidal plasma current in a toroidal geometry are investigated by using analytic model fields in order to search for the conditions of occurence of appropriate confinement asymmetry of fast electrons along the field lines. This asymmetry efficiently and quickly generates a toroidal current, which may close the field lines in ECH-started plasmas. It is analytically shown that the characteristics of confinement asymmetry depend on the product of the major radius and the strength of the external vertical field. The results suggest that in large devices By should be lowered and also some artificial means to speed up the pitch angle scattering for the fast electrons at a higher energy range are beneficial to generate the toroidal current in order to close the filed

关键词： ECH closed flux surface electron orbit

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Foreword to the Special Issue on Micro- and Nano-Metrology in Japan(I)

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Nanomanufacturing and Metrology 2021年第2期4卷 1-2页

作者： Yuki Shimizu Yasuhiro Mizutani masaki Michihata Department of Finemechanics Tohoku UniversitySendai 9808579Japan Department of Mechanical Engineering Osaka UniversitySuita 5650871Japan Department of Precision Engineering The University of TokyoBunkyo 1138654Japan

Micro-and nano-metrology occupies an important position in modern production engineering where higher-precision machining is required.A visualization of the process is indispensable for the creation of new technologies in stateof-the-art manufacturing that deals with the geometrical quantity in micrometers/*** micro-and nano-metrology is thus becoming more and more important as the platform of various manufacturing technologies,as well as strategic tools for responding to rapidly changing fabrication techniques in many scientific and industrial fields.

关键词： metrology becoming Japan

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Quantifying Roll Feel of a Car by Using a Musculoskeletal Mathematical Model

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Computer Modeling in Engineering & Sciences 2019年第3期118卷 493-507页

作者： masaki Izawa Ryota Araki Tatsuro Suzuki Kaito Watanabe Kazuhito Misaji Showa Corporation 112-1 Haga-daiHaga-machHaga-gunTochigiJapan Ex-Graduate Student Nihon UniversityCollege of Industrial TechnologyDepartment of Mathematical Information EngineeringJapan Nihon University College of Industrial TechnologyDepartment of Mathematical Information EngineeringJapan

Primary purpose of this research is to create a three-dimensional musculoskeletal mathematical model of a driver of a car using a motion capture *** model is then used in an analysis of drive torque around joints and attached muscles as a vehicle travels in different travel modes and damping force settings to examine‘burdens’for the *** studies proposed a method of quantifying the degree of musculoskeletal load in simple human motion from the changes in drive torque around joints and attached ***,examination of the level of burdens for the driver while driving using this method does not seem to *** within the scope of evaluating different travel modes and dampers,there is no other *** of this study are hence a valuable new perspective on quantitative analysis of the burdens for the driver’s joints and muscles depending on the different settings of travel modes and dampers.

关键词： Human engineering biomechanics musculoskeletal mathematical model driving torque motion capture

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