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World Journal of Gastroenterology 2005年第22期11卷 3385-3391页

作者： Atsushi Masamune masahiro Satoh Kazuhiro Kikuta Noriaki Suzuki Tooru Shimosegawa Division of Gastroenterology Tohoku University Graduate School of Medicine Sendai Japan

AM: To clarify the role of Janus kinase-signal transducers and activators of transcription (JAK-STAT) pathway in platelet-derived growth factor (PDGF) induced proliferation in activated pancreatic stellate cells (PSCs). METHODS: PSCs were isolated from rat pancreas tissue, and used in their culture-activated, myofibroblast-like phenotype. STAT-specific binding activity was assessed by electrophoretic mobility shift assay. Activation of Src, JAK2, STAT1, STAT3, and ERK was determined by Western blotting using anti-phosphospecific antibodies. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2'-deoxyuridine. RESULTS: PDGF-BB induced STAT-specific binding activity, and activation of Src, JAK2, STAT1, STAB, and ERK. Ethanol and acetaldehyde at clinically relevant concentrations decreased basal activation of JAK2 and STAT3. PDGF-induced activation of STAT1 and STAT3 was inhibited by a Src inhibitor PP1 and a JAK2 inhibitor AG490, whereas PDGF-induced activation of ERK was inhibited by PP1, and not by AG490. PDGF-induced proliferation was inhibited by PP1 and AG490 as well as by STAT3 antisense oligonucleotide. CONCLUSION: PDGF-BB activated JAK2-STAT pathway via Src-dependent mechanism. Activation of 3AK2-STAT3 pathway, in addition to ERK, may play a role in PDGF-induced proliferation of PSCs.

关键词： Pancreatitis Pancreatic fibrosis Pancreatic stellate cells Platelet-derived growth factor Janus kinase Signal transducers and activators of transcription

Does a combined intervention program of repetitive transcranial magnetic stimulation and intensive occupational therapy affect cognitive function in patients with post-stroke upper limb hemiparesis?

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Neural Regeneration Research 2016年第12期11卷 1932-1939页

作者： Takatoshi Hara masahiro Abo Kiyohito Kakita Takeshi Masuda Ryunosuke Yamazaki Department of Rehabilitation Medicine The Jikei University School of Medicine Department of Rehabilitation Medicine Kyoto Ohara Memorial Hospital

Low-frequency repetitive transcranial magnetic stimulation（LF-r TMS） to the contralesional hemisphere and intensive occupational therapy（i OT） have been shown to contribute to a significant improvement in upper limb hemiparesis in patients with chronic stroke. However, the effect of the combined intervention program of LF-r TMS and i OT on cognitive function is unknown. We retrospectively investigated whether the combined treatment influence patient＇s Trail-Making Test part B（TMT-B） performance, which is a group of easy and inexpensive neuropsychological tests that evaluate several cognitive functions. Twenty-five patients received 11 sessions of LF-r TMS to the contralesional hemisphere and 2 sessions of i OT per day over 15 successive days. Patients with right- and left-sided hemiparesis demonstrated significant improvements in upper limb motor function following the combined intervention program. Only patients with right-sided hemiparesis exhibited improved TMT-B performance following the combined intervention program, and there was a significant negative correlation between Fugl-Meyer Assessment scale total score change and TMT-B performance. The results indicate the possibility that LF-r TMS to the contralesional hemisphere combined with i OT improves the upper limb motor function and cognitive function of patients with right-sided hemiparesis. However, further studies are necessary to elucidate the mechanism of improved cognitive function.

关键词： nerve regeneration stroke repetitive transcranial magnetic stimulation Trail-Making Test cognitive function occupational therapy neural regeneration

The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability

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Protein & Cell 2011年第9期2卷 755-763页

作者： Kentaro Ito Akinori Takahashi masahiro Morita Toru Suzuki Tadashi Yamamoto Division of Oncology Department of Cancer BiologyInstitute of Medical ScienceUniversity of TokyoTokyo 108-8639Japan Cell Signal Unit Okinawa Institute of Science and Technology1919-1Okinawa 904-0412Japan

The human CCR4-NOT deadenylase complex consists of at least nine enzymatic and non-enzymatic *** evidence suggests that the non-enzymatic subunits are involved in the regulation of mRNA deadenylation,although their precise roles remain to be *** this study,we addressed the function of the CNOT1 subunit by depleting its expression in HeLa *** cytometric analysis revealed that the sub G1 fraction was increased in CNOT1-depleted ***,the same level of the sub G1 fraction was seen when cells were treated with a mixture of siRNAs targeted against all enzymatic subunits,suggesting that CNOT1 depletion induces apoptosis by destroying the CCR4-NOT-associated deadenylase *** analysis revealed that CNOT1 depletion leads to a reduction in the amount of other CCR4-NOT ***,the specific activity of the CNOT6L immunoprecipitates-associated deadenylase from CNOT1-depleted cells was less than that from control *** formation of P-bodies,where mRNA decay is reported to take place,was largely suppressed in CNOT1-depleted ***,CNOT1 has an important role in exhibiting enzymatic activity of the CCR4-NOT complex,and thus is critical in control of mRNA deadenylation and mRNA *** further showed that CNOT1 depletion enhanced CHOP mRNA levels and activated caspase-4,which is associated with endoplasmic reticulum ER stress-induced *** together,CNOT1 depletion structurally and functionally deteriorates the CCR4-NOTcomplex and induces stabilization of mRNAs,which results in the increment of translation causing ER stress-mediated *** conclude that CNOT1 contributes to cell viability by securing the activity of the CCR4-NOT deadenylase.

关键词： deadenylation CCR4-NOT small interfering RNA P-bodies apoptosis

Green tea polyphenol epigallocatechin-3-gallate blocks PDGF-induced proliferation and migration of rat pancreatic stellate cells

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World Journal of Gastroenterology 2005年第22期11卷 3368-3374页

作者： Atsushi Masamune Kazuhiro Kikuta masahiro Satoh Noriaki Suzuki Tooru Shimosegawa Division of Gastroenterology Tohoku University Graduate School of Medicine Sendai Japan

AIM: To clarify the effects of epigallocatechin-3-gallate (EGCG) on the platelet-derived growth factor (PDGF)-BB-induced proliferation and migration of pancreatic stellate cells (PSCs). METHODS: PSCs were isolated from rat pancreas tissue and used in their culture-activated, myofibroblast-like phenotype. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2'-deoxyuridine. Cell migration was assessed using modified Boyden chambers. Cyclin D1, p21waf1, and p27kip1 expression and phosphorylation of PDGF β-receptor, extracellular signal-regulated kinase, and Akt were examined by Western blotting. Activation of phospha-tidylinositol 3-kinase was examined by kinase assay using phosphatidylinositol as a substrate. Cell cycle was assessed by flow cytometry after staining with propidium iodide. RESULTS: EGCG at non-cytotoxic concentrations inhibited PDGF-induced proliferation and migration. This effect was associated with the inhibition of cell cycle progression beyond the G1 phase, decreased cyclin Dl and increased p27kip1 expression. EGCG inhibited tyrosine phosphorylation of PDGF p-receptor and downstream activation of extracellular signal-regulated kinase and phosphatidylinositol 3-kinase/ Akt pathways. CONCLUSION: EGCG inhibited PDGFBB-induced proliferation and migration of PSCs through the inhibition of PDGFmediated signaling pathways.

关键词： Pancreatitis Pancreatic fibrosis Pancreatic stellate cells Epigallocatechin-3-gallate Green tea Platelet-derived growth factor Proliferation Migration

Are direct-acting antivirals against hepatitis C virus infection not associated with the recurrence of hepatocellular carcinoma?

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Hepatobiliary Surgery and Nutrition 2019年第4期8卷 423-425页

作者： masahiro Ogawa Tatsuo Kanda Mitsuhiko Moriyama Division of Gastroenterology and Hepatology Department of Medicine Nihon University School of Medicine Tokyo Japan

Hepatitis C virus (HCV) infection is one of the major causes of end-stage liver disease and hepatocellular carcinoma (HCC). Before the use of direct acting antivirals (DAAs) against HCV, liver transplantation was one of the best ways to achieve long-term survival and a better prognosis in patients with HCV infection and advanced liver fibrosis (1). In the interferon era, it is difficult for HCV infected patients with advanced liver fibrosis to achieve sustained virological response (SVR) because interferon therapy causes severe adverse events and these completely hamper this treatment in these patients.

关键词： interferon infection hepatocellular

Airflow adjustment and minimization of the air power of ventilation network

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Journal of Coal Science & Engineering(China) 2011年第3期17卷 237-242页

作者： INOUE masahiro YANG Wen-yu Department of Earth Resources Engineering Kyushu University Japan

It is often required to know which roadway （adjustment roadway） resistances and how much values of the resis- tances should be changed to make the airflow rates in roadways （target roadways） to certain required values in the practice of mine ventilation. In this case, the airflow rates of the target roadways and the resistances of the roadways other than the ad- justment roadways are the given conditions and the resistances of the adjustment roadways are the solutions to be found. No straightforward method to solve the problem has been found up to now. Therefore, trial and error method using the ventilation network analysis program is utilized to solve the problem so far. The method takes long calculation time and the best answer is not necessarily obtained. The authors newly defined ＂airflow element＂ as an element of the ventilation network analysis. The resistances that satisfy the airflow requirements can be calculated straight forwardly by putting the function of the airflow element into the ventilation network analysis. The air power required for the ventilation can be minimized while meeting the airflow requirements by the advanced application of the method. The authors made the computer program fulfill the method. The program was applied to actual ventilation network and it was found that the method is very practical and the time required for the analysis is short.

关键词： airflow adjustment mine ventilation airflow resistance optimization

Family medicine: Global health in practice

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Family Medicine and Community Health 2015年第3期3卷 37-40页

作者： masahiro J.Morikawa Case Western Reserve University 11100 Euclid AvenueClevelandOH 44106-5036USA

For nearly 40 years,family medicine has been providing guiding principles in major global health *** article has two aims:(1)to review past major global health campaigns with a specific focus on the roles of family medicine to illustrate its relevance in global health,and(2)to address practical approaches to attain health,which nurture sustainability of local health resources and resilience of the *** of the differences in the goals of past global health campaigns,three key approaches have emerged as essential interventions in the field based on family medicine principles,the so-called six *** key approaches are(1)to protect families,(2)to train health care providers,and(3)to empower *** medicine continues to be a powerful tool to improve people’s health in our own communities and in global health practice.

关键词： Family medicine primary care global health

Decreased incidence followed by comeback of pediatric infections during the COVID‑19 pandemic in Japan

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World Journal of Pediatrics 2022年第8期18卷 564-567页

作者： Cyrus Ghaznavi Haruka Sakamoto Takayuki Kawashima Sayaka Horiuchi masahiro Ishikane Sarah Krull Abe Daisuke Yoneoka Akifumi Eguchi Yuta Tanoue masahiro Hashizume Shuhei Nomura Department of Health Policy and Management School of MedicineKeio University35 ShinanomachiShinjuku-kuTokyo 160-8582Japan Medical Education Program Washington University School of Medicine in St.LouisSaint LouisUSA Department of Global Health Policy Graduate School of MedicineThe University of TokyoTokyoJapan Tokyo Foundation for Policy Research TokyoJapan Department of Hygiene and Public Health Tokyo Women’s Medical UniversityTokyoJapan Department of Mathematical and Computing Science Tokyo Institute of TechnologyTokyoJapan Center for Birth Cohort Studies University of YamanashiYamanashiJapan Disease Control and Prevention Center National Center for Global Health and MedicineTokyoJapan Division of Prevention National Cancer Center Institute for Cancer ControlTokyoJapan Infectious Disease Surveillance Center National Institute of Infectious DiseasesTokyoJapan Department of Sustainable Health Science Center for Preventive Medical SciencesChiba UniversityChibaJapan Institute for Business and Finance Waseda UniversityTokyoJapan

During the coronavirus disease 2019(COVID-19)pandemic,the Japanese government implemented several infection control measures,such as states of emergency(SoE)and school closures,and encouraged universal masking and hand *** activity restrictions and improved hygiene were found to be associated with declines in the incidence of infuenza during the 2019–2020 winter season[1].Prior studies have found that the incidence of seven pediatric infections,such as pharyngoconjunctivitis and varicella,decreased during and after the school closures in Japan[2].As activity restrictions continue to be lifted,there is growing concern that pediatric infections may suddenly return given that the pool of susceptible children increased during the pandemic[3].In this study,we use national infectious disease sentinel data from January 2012 to October 2021 to assess changes in the reported number of pediatric infections in Japan during the COVID-19 *** hypothesize that the reporting of many infectious diseases decreased during the early stages of the pandemic but ultimately returned to normal when activity restrictions were lifted.

关键词： closure return suddenly

Recent update on electrochemical CO_(2)reduction catalyzed by metal sulfide materials

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Materials Reports(Energy) 2023年第2期3卷 103-123,I0003页

作者： An Niza El Aisnada masahiro Miyauchi Min Liu Akira Yamaguchi Department of Materials Science and Engineering School of Materials and Chemical TechnologyTokyo Institute of Technology2-12-1 OokayamaMeguro-kuTokyo152=8552Japan Biofunctional Catalyst Research Team RIKEN Center for Sustainable Resource Science2=1 HirosawaWakoSaitama351-0198Japan Hunan Joint International Research Center for Carbon Dioxide Resource Utilization State Key Laboratory of Powder MetallurgySchool of Physical and ElectronicsCentral South UniversityChangsha410083China

Seeking and developing efficient CO_(2)reduction reaction(CO_(2)RR)electrocatalysts is a hot topic in this era of global *** material candidates for sustainable and cost-effective applications,metal sulfides have attracted attention as promising nature-inspired materials due to multiple adsorption sites which are enhanced by the covalent character of *** article summarizes the current status regarding the utilization and development of metal sulfide materials as CO_(2)RR ***,the research background and basic principles of electrochemical CO_(2)RR are ***,an overview of the main obstacles to developing efficient CO_(2)RR electrocatalysts is *** section is followed by a summary of the empirical evidence supporting the application of metal sulfides as CO_(2)RR electrocatalysts beside nature-inspired *** summary of synthesis methods of various metal sulfides is also ***,the paper also highlights the recent works on metal sulfide as efficient CO_(2)RR including the undertaking strategy on the activity enhancement,and finally,discusses the challenges and prospect of metal sulfides-based CO_(2)RR *** recent efforts,metal sulfides remain relatively unexplored as materials for CO_(2)RR electrocatalytic ***,this review aims to stimulate novel ideas and research for improved catalyst designs and functionality.

关键词： Metal sulfides Electrocatalyst Electrochemical CO_(2)reduction Bi-metal sulfides Active site Defect engineering Selectivity Faradaic efficiency

High-precision group-delay dispersion measurements of optical fibers via fingerprint-spectral wavelength-to-time mapping

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Photonics Research 2016年第1期4卷 13-16页

作者： Takashi Ito Ondrej Slezak masahiro Yoshita Hidefumi Akiyama Yohei Kobayashi Institue for Solid State Physics University of Tokyo HiLASE Centre Institute of Physics ASCR

The group-delay dispersion of an optical fiber was measured with the time-of-flight method, using fingerprint-like characteristic spectra from a mode-locked fiber laser source. To determine the group-delay dispersion up to the fourth order, least-squares fitting was applied to the overall time waveform mapped on the time axis for the fingerprint-spectral broadband pulses through a long optical fiber. The analysis of all 4003 data points reduced statistical uncertainty, and provided second-, third-, and fourth-order dispersion with uncertainties of 0.02%, 0.4%, and 4%,respectively.

关键词： length time High-precision group-delay dispersion measurements of optical fibers via fingerprint-spectral wavelength-to-time mapping