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含DNA嵌段的共聚物的合成与溶液自组装

含DNA嵌段的共聚物的合成与溶液自组装

DNA功能化的共聚物的合成与胶束化行为研究

2012年全国高分子材料科学与工程研讨会

作者：潘鹏举 masahiro fujita Mizuo Maeda 浙江大学化工系化学工程联合国家重点实验室 Bioengineering Laboratory RIKEN

利用ATRP和点击化学,合成了线性和星形的聚异丙基丙烯酰胺（PNIPAAm）/DNA的共聚物,并且研究了其在水溶液中的胶束化行为。与PNIPAAm均聚物相比,亲水性DNA链段的引入使PNIPAAm在水溶液中的最低临界溶解温度（LCST）升高,相转变过程中焓... 详细信息

利用ATRP和点击化学,合成了线性和星形的聚异丙基丙烯酰胺（PNIPAAm）/DNA的共聚物,并且研究了其在水溶液中的胶束化行为。与PNIPAAm均聚物相比,亲水性DNA链段的引入使PNIPAAm在水溶液中的最低临界溶解温度（LCST）升高,相转变过程中焓变减小。在LCST温度以上,PNIPAAm-co-DNA共聚物发生胶束化,形成了以PNIPAAm疏水性链段为核、ssDNA亲水性链段为壳的胶束粒子。共聚物的组成和拓扑结构是影响胶束粒子尺寸和结构的主要因素。该类DNA修饰的软纳米粒子在水溶液中的稳定性对目标ssDNA的序列非常敏感,在基因检测中将有潜在的应用前景。

关键词： DNA 聚异丙基丙烯酰胺原子转移自由基聚合点击化学胶束

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DNA功能化的共聚物的合成与胶束化行为研究

Forming of magnesium alloy by underwater shock wave

2011年全国高分子学术论文报告会

作者：潘鹏举 masahiro fujita Mizuo Maeda 浙江大学化工系化学工程国家联合重点实验室 Bioengineering Laboratory RIKENHirosawa 2-1WakoSaitama 351-0198Japan

＜正＞DNA接枝修饰的无机纳米粒子已经广泛应用于基因的快速检测、生物传感器等领域。结合原子转移自由基聚合（ATRP）和点击化学的方法,我们合成了线性两嵌段和星形的聚异丙基丙烯酰胺（PNIPAAm）/单螺旋

关键词： DNA 聚异丙基丙烯酰胺原子转移自由基聚合点击化学胶束

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Journal of Magnesium and Alloys 2016年第1期4卷 27-29页

作者： Liqun Ruan Shunsuke Ezaki fujita masahiro Shirley Shen Yosihito Kawamura Kumamoto University Magnesium Research Center(MRC) Kurokami 2-39-1KumamotoPostcode 860-8555 Japan Department of Mechanical Systems Engineering Graduate School of Science and Technology Kumamoto UniversityKurokami 2-39-1Kumamoto-shi Postcode 860-8555Japan Soujyou University Kumamoto-shiJapan CSIRO Manufacturing Flagship Private Bag 10Clayton South 3149Australia

Magnesium alloys are best known for their light weight and high strength-to-weight ratio.In the forging process of magnesium alloys,hot working of has been often carried out.However,the strength of the material is reduced because the material temperature reaches the recrystallization temperature during hot working.Therefore,the cold method working is required to maintain or increase the strength of a material.In this study,we have designed experimental equipment to examine whether the forming of magnesium alloy is possible in the cold working by utilizing underwater shock wave,and the hardness of the material changes by receiving the underwater shock wave.Experimental results revealed increase in coefficient of extension and hardness of materials after receiving a shock wave.

关键词： Magnesium alloy Cold working Shock wave Forging Coefficient of extension Vickers hardness

IGF2 differentially methylated region hypomethylation in relation to pathological and molecular features of serrated lesions

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World Journal of Gastroenterology 2014年第29期20卷 10050-10061页

作者： Takafumi Naito Katsuhiko Nosho Miki Ito Hisayoshi Igarashi Kei Mitsuhashi Shinji Yoshii Hironori Aoki Masafumi Nomura Yasutaka Sukawa Eiichiro Yamamoto Yasushi Adachi Hiroaki Takahashi Masao Hosokawa masahiro fujita Toshinao Takenouchi Reo Maruyama Hiromu Suzuki Yoshifumi Baba Kohzoh Imai Hiroyuki Yamamoto Shuji Ogino Yasuhisa Shinomura Department of Gastroenterology Rheumatology and Clinical ImmunologySapporo Medical University School of Medicine Department of Gastroenterology Keiyukai Sapporo Hospital Center for Gastroenterology Teine-Keijinkai Hospital Department of Medical Oncolo-gy Dana-Farber Cancer InstituteBostonMA 02115United States Department of Surgery Keiyukai SapporoHospital Department of Pathology Keiyukai Sapporo Hospital Department of Molecular Biology Sapporo Medical University School of Medicine Department of Gastroenterological Surgery Graduate School of Medical ScienceKumamoto University Division of Cancer Research The Institute of Medical ScienceThe University of Tokyo Division of Gastroenterology and Hepatology Department of Internal MedicineSt.Marianna University School of Medicine

AIM:To investigate insulin-like growth factor 2(IGF2)differentially methylated region(DMR)0 hypomethylation in relation to clinicopathological and molecular features in colorectal serrated lesions.METHODS:To accurately analyze the association between the histological types and molecular features of each type of serrated lesion,we consecutively collected1386 formalin-fixed paraffin-embedded tissue specimens that comprised all histological types[hyperplastic polyps(HPs,n=121),sessile serrated adenomas(SSAs,n=132),traditional serrated adenomas(TSAs,n=111),non-serrated adenomas(n=195),and colorectal cancers(n=827)].We evaluated the methylation levels of IGF2 DMR0 and long interspersed nucleotide element-1(LINE-1)in HPs(n=115),SSAs(n=120),SSAs with cytological dysplasia(n=10),TSAs(n=91),TSAs with high-grade dysplasia(HGD)(n=15),non-serrated adenomas(n=80),non-serrated adenomas with HGD(n=105),and CRCs(n=794).For the accurate quantification of the relative methylation levels(scale 0%-100%)of IGF2 DMR0 and LINE-1,we used bisulfite pyrosequencing method.Tumor specimens were analyzed for microsatellite instability,KRAS(codons 12 and 13),BRAF(V600E),and PIK3CA(exons 9and 20)mutations;MLH1 and MGMT methylation;and IGF2 expression by immunohistochemistry.RESULTS:The distribution of the IGF2 DMR0 methylation level in 351 serrated lesions and 185 non-serrated adenomas(with or without HGD)was as follows:mean61.7,median 62.5,SD 18.0,range 5.0-99.0,interquartile range 49.5-74.4.The IGF2 DMR0 methylation level was divided into quartiles(Q1≥74.5,Q2 62.6-74.4,Q3 49.6-62.5,Q4≤49.5)for further analysis.With regard to the histological type,the IGF2 DMR0 methylation levels of SSAs(mean±SD,73.1±12.3)were significantly higher than those of HPs(61.9±20.5),TSAs(61.6±19.6),and non-serrated adenomas(59.0±15.8)(P<0.0001).The IGF2 DMR0 methylation level was inversely correlated with the IGF2 expression level(r=-0.21,P=0.0051).IGF2 DMR0 hypomethylation was less frequently detected in SSAs compared with HPs,TSAs,and non-serrated adenomas(P<0.0001

关键词： BRAF Colon polyp Colorectal neoplasia Colorectum G

Two cases of pancreatic ductal adenocarcinoma with intrapancreatic metastasis

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World Journal of Gastroenterology 2016年第41期22卷 9222-9228页

作者： Yusuke fujita Minoru Kitago Yohei Masugi Osamu Itano masahiro Shinoda Yuta Abe Taizo Hibi Hiroshi Yagi Yoko Fujii-Nishimura Michiie Sakamoto Yuko Kitagawa Department of Surgery Keio University School of Medicine Department of Pathology Keio University School of Medicine

There are no standardized diagnostic criteria for intrapancreatic metastasis of pancreatic ductal adenocarcinoma(PDAC). Here, we report two cases of patients with PDAC who were pathologically diagnosed as harboring intrapancreatic metastasis. In both cases, the main lesions were located in the pancreatic body, and no other lesion was detected preoperatively. The patients were diagnosed with pancreatic body cancers and distal pancreatectomy was performed. Pathological findings revealed microscopic cancer nests, which had connections to neither the main lesion nor the premalignant lesion in the pancreatic tail parenchyma. In both cases, the histological type of the daughter lesion was quite similar to that of the main lesion. Hence, we diagnosed the daughter lesions as metastatic foci in the pancreas. Although intrapancreatic metastasis of PDAC has been regarded as a poor prognostic factor, few reports of intrapancreatic metastasis are available. This article reports two such cases and provides a review of the literature.

关键词：癌胰腺的 ductal 瘤 micrometastasis 复发 Carcinogenesis

Recent evidence for subcutaneous drains to prevent surgical site infections after abdominal surgery:A systematic review and metaanalysis

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World Journal of Gastrointestinal Surgery 2023年第12期15卷 2879-2889页

作者： Tomohiro Ishinuki Hiroji Shinkawa Keita Kouzu Seiichi Shinji Erika Goda Toshio Ohyanagi masahiro Kobayashi Motomu Kobayashi Katsunori Suzuki Yuichi Kitagawa Chizuru Yamashita Yasuhiko Mohri Junzo Shimizu Motoi Uchino Seiji Haji masahiro Yoshida Hiroki Ohge Toshihiko Mayumi Toru Mizuguchi Department of Nursing Surgical SciencesSapporo Medical UniversitySapporo 060-8556HokkaidoJapan Department of Hepatobiliary-Pancreatic Surgery Osaka Metropolitan University Graduate School of MedicineAbeno-Ku 545-0051Japan Department of Surgery National Defense Medical CollegeTokorozawa 359-8513Japan Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Nippon Medical SchoolTokyo 113-8602Japan Department of Nursing Japan Health Care UniversitySapporo 062-0053Japan Department of Liberal Arts and Sciences Center for Medical EducationSapporo Medical UniversitySapporo 060-8556Japan Department of Clinical Pharmacokinetics Research and Education Center for Clinical PharmacyKitasato UniversityTokyo 108-8641Japan Department of Anesthesiology Hokushinkai Megumino HospitalEniwa 061-1395Japan Department of Infectious Disease Medicine School of MedicineUniversity of Occupational and Environmental HealthKitakyushu 807-8555Japan Department of Gastrointestinal Surgery National Center for Geriatrics and GerontologyObu 474-8511Japan Department of Anesthesiology and Critical Care Medicine Fujita Health UniversityToyoake 470-1192Japan Department of Gastrointestinal Surgery Mie Prefectural General Medical CenterYokkaich 510-8561Japan Department of Surgery Toyonaka Municipal HospitalToyonaka 560-8565Japan Department of Gastroenterological Surgery Hyogo Medical UniversityNishinomiya 663-8501Japan Department of Surgery Soseikai General HospitalKyoto 612-8473Japan Department of Hepato-Biliary-Pancreatic&Gastrointestinal Surgery International University of Health and WelfareIchikawa 272-0827Japan Department of Infectious Disease Hiroshima University HospitalHiroshima 734-8551Japan Department of Intensive Care Unit Chukyo HospitalJapan Community Health Care OrganizationNagoya 457-8510Japan

BACKGROUND Surgical site infections(SSIs)increase mortality,hospital stays,additional medical treatment,and medical costs.Subcutaneous drains prevent SSIs in gynecological and breast surgeries;however,their clinical impact in abdominal surgery remains unclear.AIM To investigate whether subcutaneous drains were beneficial in abdominal surgery using a systematic review and meta-analysis.METHODS The database search used PubMed,MEDLINE,and the Cochrane Library.The following inclusion criteria were set for the systematic review:(1)Randomized controlled trial studies comparing SSIs after abdominal surgery with or without subcutaneous drains;and(2)Studies that described clinical outcomes,such as SSIs,seroma formation,the length of hospital stays,and mortality.RESULTS Eight studies were included in this meta-analysis.The rate of total SSIs was significantly lower in the drained group(54/771,7.0%)than in the control group(89/759,11.7%),particularly in gastrointestinal surgery.Furthermore,the rate of superficial SSIs was slightly lower in the drained group(31/517,6.0%)than in the control group(49/521,9.4%).No significant differences were observed in seroma formation between the groups.Hospital stays were shorter in the drained group than in the control group.CONCLUSION Subcutaneous drains after abdominal surgery prevented SSIs and reduced hospital stays but did not significantly affect seroma formation.The timing of drain removal needs to be reconsidered in future studies.

关键词： Abdominal surgery Mortality Seroma formation Subcutaneous drain Surgical site infections

Case series of three patients with hereditary diffuse gastric cancer in a single family:Three case reports and review of literature

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World Journal of Gastroenterology 2020年第42期26卷 6689-6697页

作者： masahiro Hirakawa Kohichi Takada Masanori Sato Chisa fujita Naotaka Hayasaka Takayuki Nobuoka Shintaro Sugita Aki Ishikawa Miyako Mizukami Hiroyuki Ohnuma Kazuyuki Murase Koji Miyanishi Masayoshi Kobune Ichiro Takemasa Tadashi Hasegawa Akihiro Sakurai Junji Kato Department of Medical Oncology Sapporo Medical University School of MedicineSapporo 060-8556HokkaidoJapan Department of Gastroenterology National Hospital Organization Hokkaido Cancer CenterSapporo 003-0804HokkaidoJapan Department of Surgery Surgical Oncology and ScienceSapporo Medical University School of MedicineSapporo 060-8556HokkaidoJapan Department of Surgical Pathology Sapporo Medical University School of MedicineSapporo 060-8556HokkaidoJapan Department of Medical Genetics Sapporo Medical University School of MedicineSapporo 060-8556HokkaidoJapan Department of Hematology Sapporo Medical University School of MedicineSapporo 060-8556HokkaidoJapan

BACKGROUND Hereditary diffuse gastric cancer(HDGC)is a familial cancer syndrome often associated with germline mutations in the CDH1 gene.However,the frequency of CDH1 mutations is low in patients with HDGC in East Asian countries.Herein,we report three cases of HDGC harboring a missense CDH1 variant,c.1679C>G,from a single Japanese family.CASE SUMMARY A 26-year-old female(Case 1)and a 51-year-old male(father of Case 1),who had a strong family history of gastric cancer,were diagnosed with advanced diffuse gastric cancer.After genetic counselling,a 25-year-old younger brother of Case 1 underwent surveillance esophagogastroduodenoscopy that detected small signet ring cell carcinoma foci as multiple pale lesions in the gastric mucosa.Genetic analysis revealed a CDH1 c.1679C>G variant in all three patients.CONCLUSION It is important for individuals suspected of having HDGC to be actively offered genetics evaluation.This report will contribute to an increased awareness of HDGC.

关键词： Hereditary diffuse gastric cancer Signet ring cell carcinoma CDH1 Ecadherin Endoscopic findings Case report

Wind Tunnel Test for Videogrammetric Deformation Measurement of UAV for Mars Airplane Balloon Experiment-1 (MABE-1)

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Journal of Flow Control, Measurement & Visualization 2019年第2期7卷 87-100页

作者： Koji fujita Akira Oyama Daisuke Kubo masahiro Kanazaki Hiroki Nagai Institute of Fluid Science Tohoku University Sendai Japan Institute of Space and Astronautical Science Japan Aerospace Exploration Agency Sagamihara Japan Japan Aerospace Exploration Agency Mitaka Japan Tokyo Metropolitan University Hino Japan

This paper reports the results of the aerodynamic deformation measurements of the meter-scale, entire shape, actual UAV in the wind tunnel using a video grammetry technique. The measured airplane was the airplane for Mars exploration being developed by Japan Aerospace Exploration Agency (JAXA) and Japanese universities. Its main wing span length was 2.4 m. The video grammetry measurement was performed using VICON’s system. Retroactive markers and stickers were put on the airplane. JAXA’s 6.5 m × 5.5 m Low-Speed Wind Tunnel was used. The airplane was mounted on the strut support with pitch-free or pitch-locked conditions. The deformations of the main wing bending, the main wing twisting, the tail boom bending, and the elevator deflection angle change were revealed quantitatively. The bending stiffness of a main wing spar that was designed as a safety factor of 2.8 at load factor of 5 was sufficient. The main wing spar was located around a center of pressure of an airfoil and it showed enough stiffness for twisting at nominal condition. The effects of the main wing bending and twisting, and the tail boom bending on the aerodynamic performance were estimated but they were in an acceptable range from the standpoint of the controllability of the aerodynamic performance using control surfaces. Even though the servo motor was located near the elevator and the linkage between the servo motor and the elevator was short, the measured elevator deflection angle was at most 4% smaller than the angle at no-wind condition. The obtained results and presented method are useful for control, flight data analysis, and design of lightweight airplanes.

关键词： Wind Tunnel UAV Deformation Video Grammetry Mars Exploration

Cutting Characteristics of Duplex Stainless Cast Steel X2CrNiMoN25-7-3

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Journal of Mechanics Engineering and Automation 2017年第6期7卷 300-306页

作者： masahiro Hagino Akihiro Takemura Tsuyoshi fujita Hiroshi Usuki Akihiko Ikuta The Department of Integrated Science and Technology National Institute of Technology Tsuyama College Tsuyama 708-8509 Japan The Department of Mechanical Engineering National Institute of Technology Yonago College Yonago 683-8502 Japan The Department of Interdisciplinary Faeulty of Science and Engineering Shimane University Matsue 690-8504 Japan The Department of Mechanical Engineering Kindai University Higashihiroshima 739-2116 Japan

因为它有优秀 pitting 腐蚀抵抗， X2CrNiMoN25-7-3 双不锈钢对象海水那样的包含氯化物的环境合适。双纯洁的演员组钢经常被用来在关节提供部分几何学的复杂性。然而，在扔以后用机器制造是不可缺少的。这研究评估了纯洁的演员组钢的切... 详细信息

因为它有优秀 pitting 腐蚀抵抗， X2CrNiMoN25-7-3 双不锈钢对象海水那样的包含氯化物的环境合适。双纯洁的演员组钢经常被用来在关节提供部分几何学的复杂性。然而，在扔以后用机器制造是不可缺少的。这研究评估了纯洁的演员组钢的切的特征。优势的粘附在高切速度，而是工具是弱的穿大。在细工品和工具边之间的散开和反应被调查。内部散开的忽视和一个反应阶段被观察，但是契约能力是低的。

关键词：双不锈钢 sintered 碳化物工具金属陶瓷工具马赫无能纯洁的演员组钢粘附