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Validation of SNP markers for fruit quality and disease resistance loci in apple (Malus × domestica Borkh.) using the OpenArray® platform

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Horticulture Research 2019年第1期6卷 1348-1363页

作者： David Chagné Stijn Vanderzande Chris Kirk Natalie Profitt Rosemary Weskett Susan e.Gardiner Cameron P.Peace Richard K.Volz Nahla V.Bassil The New Zealand Institute for Plant&Food Research Ltd(Plant&Food Research) Palmerston North Research CentrePalmerston NorthNew Zealand Department of Horticulture Washington State UniversityPullmanWAUSA Plant&Food Research Hawke’s Bay Research CentreHavelock NorthNew Zealand USDA-ARS National Clonal Germplasm RepositoryCorvallisORUSA

Genome mapping has promised much to tree fruit breeding during the last 10 ***,one of the greatest challenges remaining to tree fruit geneticists is the translation of trait loci and whole genome sequences into diagnostic genetic markers that are efficient and cost-effective for use by breeders,who must select genetically optimal parents and subsequently select genetically superior individuals among their *** take this translational step,we designed the apple International RosBReeD SNP Consortium OpenArray v1.0(IRSCOA v1.0)assay using a set of 128 apple single nucleotide polymorphisms(SNPs)linked to fruit quality and pest and disease resistance trait *** Thermo Fisher Scientific OpenArray®technology enables multiplexed screening of SNP markers using a real-time PCR instrument with fluorescent probe-based Taqman®*** validated the apple IRSCOA v1.0 multitrait assay by screening 240 phenotyped individuals from the Plant&Food Research apple cultivar breeding *** set of individuals comprised commercial and heritage cultivars,elite selections,and families segregating for traits of importance to *** total,33 SNP markers of the IRSCOA v1.0 were validated for use in marker-assisted selection(mas)for the scab resistances Rvi2/Vh2,Rvi4/Vh4,Rvi6/Vf,fire blight resistance MR5/RLP1,powdery mildew resistance Pl2,fruit firmness,skin colour,flavour intensity,and *** availability of this set of validated trait-associated SNP markers,which can be used individually on multiple genotyping platforms available to various apple breeding programmes or re-designed using the flanking sequences,represents a large translational genetics step from genomics to crop improvement of apple.

关键词： Open resistance breeding

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眼附属器结外边缘区B细胞淋巴瘤的浆细胞分化:分析肿瘤的浆细胞分型及136例患者的预后重要性

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世界核心医学期刊文摘（眼科学分册） 2005年第8期1卷 46-47页

作者： Coupland S.e. Hellmich M. Auw-Haedrich 王海燕 Institute of Pathology Char'.-Medical Faculty Berlin Campus Benjamin Franklin Germany Hindenburgdamm 30 D-12203 Berlin Germany Dr.

Aim: To determine (a) the expression of plasma cell related antigens in extran odal marginal zone B cell lymphomas (eMZL) of the ocular adnexa; and (b) the pro gnostic value of plasmacellular differentiation in these tumours. Methods: A con secutive case series of 136 ocular adnexal eMZL obtained from three ocular patho logy centres over 20 years was analysed retrospectively. An extensive immunohist ochemical panel, including the plasma cell related antigens VS38c, CD38, CD138, multiple myeloma oncogene-1-protein (MUM1/ IRF4), and CReB binding protein (CB P) was performed. eMZL were defined as “plasmacellular differentiated”on the b asis of morphological features, evidence of cytoplasmic immunoglobulin, negativi ty for BSAP/PAX5, and expression of at least one of the investigated plasma cell related antigens. Controls included normal or hyperplastic lymphatic tissues. D etailed clinical data were collected for most patients, and compared with the re sults of immunohistochemistry. The end points considered for statistical analysi s were development of local tumour recurrence, development of systemic disease, and lymphoma related death. Results: 57 (42%) of the 136 ocular adnexal eMZL sh owed a plasmacellular differentiation; 45 of these plasmacytoid cases were prima ry tumours. In contrast with most admixed normal plasma cells, which displayed c o-expression of MUM1/IRF4, Vs38c, CD38, CD138, and CBP, the plasmacellular diff erentiated eMZL tumour cells demonstrated co-expression of all five plasma cell related antigens in only six of 57 (11%) plasmacellular differentiated ocular adnexal eMZL. The most commonly expressed plasma cell related antigen was MUM1/I RF4, immunoreactivity being seen in 56/57 (98%) plasmacellular differentiated e MZL examined. Although the association of plasmacellular differentiation in prim ary ocular adnexal eMZL and disseminated disease was statistically significant o n univariate analysis (p=0.042), this was weaker on multivariate analysi

关键词：眼附属器浆细胞病淋巴瘤分化型多发性骨髓瘤边缘区相关抗原免疫球蛋白多因素分析癌基因

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基于多智能体的自然灾害动态风险评估模型

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地理与地理信息科学 2009年第2期25卷 85-88页

作者：王飞尹占娥温家洪 Ningchuan Xiao 上海师范大学地理系上海200234 Department of Geography the Ohio State University Columbus OH 43210 USA

将多智能体建模方法引入灾害风险评估领域,以期为灾害风险评估开拓新的研究思路。区别于一般静态评估模型,基于多智能体的风险评估模型自下而上,从微观上建立灾害系统各要素之间的相互关系,在多风险情景下,仿真模拟灾害系统状态的变化,... 详细信息

将多智能体建模方法引入灾害风险评估领域,以期为灾害风险评估开拓新的研究思路。区别于一般静态评估模型,基于多智能体的风险评估模型自下而上,从微观上建立灾害系统各要素之间的相互关系,在多风险情景下,仿真模拟灾害系统状态的变化,以实现动态的风险评估。在理解多智能体建模的一般原理的基础上,设计了基于多智能体的自然灾害动态风险评估的总体模型和主要智能体的结构,并总结了多智能建模在风险评估方面的优势。

关键词：多智能体动态风险评估自然灾害

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木薯蚕丝的13C CP/mas NMR分析

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丝绸 2021年第1期58卷 1-7页

作者：陈静黄继伟彭泽冶宁晚娥广西科技大学生物与化学工程学院广西柳州545006 苏州大学纺织与服装工程学院江苏苏州215123

木薯蚕丝作为一种极具开发利用价值的野蚕丝,其结晶结构、热学性质和力学性能显著不同于桑蚕丝,对木薯蚕丝二级结构的研究有助于进一步认识其构效关系,拓展其应用领域。13C CP/mas NMR技术是研究蚕丝丝素蛋白结构的有效方法之一。文章通... 详细信息

木薯蚕丝作为一种极具开发利用价值的野蚕丝,其结晶结构、热学性质和力学性能显著不同于桑蚕丝,对木薯蚕丝二级结构的研究有助于进一步认识其构效关系,拓展其应用领域。13C CP/mas NMR技术是研究蚕丝丝素蛋白结构的有效方法之一。文章通过13C CP/mas NMR测试,分析了木薯蚕丝丝素蛋白的二级结构特征,并将其与桑蚕丝和柞蚕丝进行了对比。结果表明:桑蚕丝中Gly的含量较柞蚕丝和木薯蚕丝高,木薯蚕丝与柞蚕丝相近;Ala的含量及其β-sheet结构较柞蚕丝和木薯蚕丝低,木薯蚕丝最高,三者的α-螺旋结构相差不大;桑蚕丝的不定形区含量最高,木薯蚕丝最低,与柞蚕丝接近。

关键词：木薯蚕丝桑蚕丝柞蚕丝丝素蛋白二级结构 13 C CP/mas NMR

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拉坦前列素导致虹膜颜色变化的研究

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世界核心医学期刊文摘（眼科学分册） 2005年第4期1卷 19-20页

作者： Arranz-Marquez e. Teus M.A. Saornil M.A. 陈立军

To study the histologic aspects of irises subjected to extended latanoprost treatment. Prospective, observer- mas- ked study. Iris biopsies of eyes treated with latanoprost were analyzed (all had a photographically documented increase in iris pigmentation) plus control eyes (untreated with prostanoids) using optical microscopy. patient or study population: There were 14 study eyes treated with latanoprost and 8 untreated control eyes. main outcome measure: The morphologic characteristics of the irises. The irises treated with latanoprost had an increased number of melanocytes with nuclear inclusions, granules of melanin in the vascular walls and the melanocytes and free granules in the stroma compared with control eyes (P=.001, P=.01, P=.004, P=.01, respectively, by the χ 2 test). Chronic therapy with latanoprost appears to induce more changes in the iris than a simple increase in the melanin content of the melanocytes.

关键词：拉坦黑色素细胞核内包涵体黑色素颗粒色素含量研究人群活检组织观测指标观察性盲法

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核膜营养不良显示促成肌肉再生Rb-MyoD途径破裂的转录指纹

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世界核心医学期刊文摘（神经病学分册） 2006年第9期2卷 38-39页

作者： Bakay M. Melcon G. e.P. Hoffman 高宗恩 Research Center for Genetic Medicine Children's National Medical Center 111 Michigan Ave NW Washington DC 20010 United StatesDr.

Mutations of lamin A/C (LMNA) cause a wide range of human disorders, including progeria, lipodystrophy, neuropathies and autosomal dominant emery-Dreifuss muscular dystrophy (eDMD). eDMD is also caused by X-linked recessive loss-of-function mutations of emerin, another component of the inner nuclear lamina that directly interacts with LMNA. One model for disease pathogenesis of LMNA and emerin mutations is cellspecific perturbations of the mRNA transcriptome in terminally differentiated cells. To test this model, we studied 125 human muscle biopsies from 13 diagnostic groups (125 U133A, 125 U133B microarrays), including eDMD patients with LMNA and emerin mutations. A Visual and Statistical Data Analyzer (VISDA) algorithm was used to statistically model cluster hierarchy, resulting in a tree of phenotypic classifications. Validations of the diagnostic tree included permutations of U133A and U133B arrays, and use of two probe set algorithms (mas5.0 and MBe1). This showed that the two nuclear envelope defects (eDMD LMNA, eDMD emerin) were highly related disorders and were also related to fascioscapulohumeral muscular dystrophy (FSHD). FSHD has recently been hypothesized to involve abnormal interactions of chromatin with the nuclear envelope. To identify disease-specific transcripts for eDMD, we applied a leave-one-out (LOO) cross-validation approach using LMNA patient muscle as a test data set, with reverse transcription-polymerase chain reaction (RT-PCR) validations in both LMNA and emerin patient muscle. A high proportion of top-ranked and validated transcripts were components of the same transcriptional regulatory pathway involving Rb1 and MyoD during muscle regeneration (CRI-1, CReBBP, NapIL1, eCReBBP/p300), where each was specifically upregulated in eDMD. Using a muscle regeneration time series (27 time points) we develop a transcriptional model for downstream consequences of LMNA and emerin mutations. We propose that key interactions between the nuclear envelope

关键词： mRNA转录脂肪营养不良肌肉再生 Rb1 MyoD 核膜面肩肱型肌营养不良常染色体显性遗传性

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体外膜式人工氧合法应用于婴儿胎粪吸入综合征:静脉-静脉体外膜式氧合法10年的经验

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世界核心医学期刊文摘（儿科学分册） 2005年第12期1卷 48-49页

作者： Kugelman A. Gangitano e. Taschuk R. M. Durand 王经纬 LAC- USC Medical Center Women's and Children's Hospital 1240 N. Mission Rd Los Angeles CA 90033 United States

Despite the emergence of new therapies for respiratory failure of the newborn with meconium aspiration syndrome (mas), extracorporeal membrane oxygenation (eCMO) has a significant role as a rescue modality in these infants. Our objective was to compare the use of venovenous (VV) vs venoarterial (VA) eCMO in newborns with mas who need eCMO and to ascertain the impact of new therapies in these infants during the last decade. We also evaluated how disease severity or time of eCMO initiation affected mortality and morbidity. Methods: A report of 12 years experience (1990- 2002) of a single center, comparing VV and VA eCMO, is given. Venovenous eCMO was the preferred rescuemodality for respiratory failure unresponsive to maximal medical therapy. Venoarterial eCMO was used only when the placement of a VV eCMO 14- F catheter was not possible; 128 patients met eCMO criteria, 114 were treated with VV eCMO, and 12 with VA eCMO. Two patients were converted from VV to VA eCMO. Results: Venovenous and VA eCMO patients had comparable birth weight (mean ± SeM, 3.48 ± 0.05 vs 3.35 ± 0.15 kg) and gestational age (40.3 ± 0.1 vs 40.7 ± 0.3 weeks). Before eCMO, there was no difference between VV and VA eCMO patients in oxygenation index (60 ± 3 vs 63 ± 8), mean airway pressure (19.5 ± 0.4 vs 20.8 ± 1.5 cm H2O), alveolar-arterial O2 gradient (630 ± 2 vs 632 ± 4 torr), eCMO cannulation age (median [25th- 75th percentiles], 23 [14- 47] vs 26 [14- 123] hours),or in the % of patients who needed vasopressors/ inotropes (98% vs 100% ). From November 1994, inhaled nitric oxide (NO) was available. Before VV eCMO, 67% of the patients received NO, 24% received surfactant, and 48% were treated with high-frequency ventilation (HFV). There was no significant difference bet-ween VV and VA eCMO patients in survival rate (94% vs 92% ), eCMO duration (88 [64- 116] vs 94 [55- 130] hours), time of extubation (9 [7- 11] vs 14 [9- 15] days), age at discharge (23 [18- 30] vs 27 [15- 41] days), or incidence o

关键词：胎粪吸入综合征体外膜式氧合膜式表面活性物质法应平均气道压呼吸衰竭颅内合并症动脉氧分压套管插入术

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阻塞性睡眠呼吸暂停综合征儿童的上呼吸道动态反应

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世界核心医学期刊文摘（儿科学分册） 2005年第5期1卷 61-62页

作者： Marcus C. L. Katz e. S. Lutz J. 王经纬 Division of Pediatric Pulmonology Wood Building Children's Hospital of Philadelphia 34th St. and C ivic Center Blvd. Philadelphia PA 19104 United States

Normal children have a smaller upper airway than adults, but, nevertheless, sn ore less and have less apnea. We have previously shown that normal children have an upper airway that is resistant to collapse during sleep. We hypothesized tha t this resistance to collapse is due to preservation of upper airway neuromotor responses during sleep. Furthermore, we hypothesized that upper airway responses would be diminished in children with the obstructive sleep apnea syndrome (OSAS ). We therefore compared the upper airway pressure-flow relationship during sle ep between children with OSAS and controls. Measurements were made by correlatin g maximal inspiratory airflow with the level of nasal pressure applied via a mas k. Neuromotor upper airway activationwas assessed by evaluating the upper airway response to 1) hypercapnia and 2) intermittent, acute negative pressure. We fou nd that children with OSAS had no significant response to either hypercapnia or negative pressure during sleep, compared with the normal children. After treatme nt of OSAS by tonsillectomy and adenoidectomy, there was a trend for normalizati on of upper airway responses. We conclude that upper airway dynamic responses ar e decreased in children with OSAS but recover after treatment. We speculate that the pharyngeal airway neuromotor responses present in normal children are a com pensatory response for a relatively narrow upper airway. Further, we speculate t hat this compensatory response is lacking in children with OSAS, most likely due to either habituation to chronic respiratory abnormalities during sleep or to m echanical damage to the upper airway.

关键词：动态反应睡眠状态呼吸暂停增殖腺切除术神经运动高碳酸血症激活性压力水平性适应机械性损伤

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基于mas的敏捷供应链谈判模型

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工业工程 2007年第1期10卷 77-82页

作者：张丽郑丕谔饶国政天津大学管理学院天津大学计算机科学与技术学院天津300072

通过分析敏捷供应链的业务流程,建立了一个多Agent的敏捷供应链的谈判模型,并将敏捷供应链双边谈判中买卖双方表示成两个谈判Agent。任选其中买方谈判Agent的状态转换过程抽象为动作规划,并把它形式化为一个模糊自动机。在分析了Agent... 详细信息

通过分析敏捷供应链的业务流程,建立了一个多Agent的敏捷供应链的谈判模型,并将敏捷供应链双边谈判中买卖双方表示成两个谈判Agent。任选其中买方谈判Agent的状态转换过程抽象为动作规划,并把它形式化为一个模糊自动机。在分析了Agent谈判的过程之后给出了谈判Agent的体系结构,并给出了该谈判模型的应用案例,为建立实用的Agent谈判系统奠定了理论依据。

关键词：多Agent谈判敏捷供应链动作规划模糊自动机

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非心脏手术行极量与次极量多巴酚丁胺负荷超声心动图试验阴性患者的心脏事件:静息室壁运动异常的意义

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世界核心医学期刊文摘（心脏病学分册） 2005年第1期1卷 40-41页

作者： Labib S.B. Goldstein M. Kinnunen P.M. Schick e.C. 苏显明 Dept. of Ca rdiovascular Medicine Lahey Clinic Burlington MA United StatesDr.

Objectives This study sought to evaluate the negative predictive value( NPV) of preoperative dobutamine stress echocardiography(DSe) in patients who fail to ac hieve target heart rate(HR) and assess the influence of resting wall motion abno rmalities(Wmas) without demonstrable ischemia on perioperative events. Backgroun d The prognostic value of a negative-submaximal DSe study before noncardiac sur gery is unknown. Methods Consecutive patients (n=429) who underwent surgery over a three-year period, preceded by DSe, were included. We compared perioperative event rates among those without inducible ischemia according to whether or not 85%age-adjusted maximum HR was achieved, and whether Wmas were present at rest . Results Of 397 negative DSes, peak HR was edicted in 62 (16%) . Most were receiving beta-blockers (77%). The average dobutamine and atropine doses were 48 μg/kg/min and 1.2 mg, respectively. Average HR was 115 beats/min (74%maximum predicted). Perioperative myocardial infarctions occurred more fre quently in patients with positive tests (3 of 32 > vs. 7 of 397 >; p =0.03) , but with similar frequency among the negative-maximal and negative-su bmaximal groups(6 of 335, 1.8%vs. 1 of 62, 1.6%, respectively). Accordingly, t he NPV was 98%in both subgroups. events occurred exclusively in patients with W mas at rest: 7 of 100 (7%) versus 0 of 297 (0%) (p ents undergoing preoperative DSe, failure to achieve target HR is not uncommo n despite an aggressive DSe regimen. A negative DSe without resting Wmas has exc ellent NPV regardless of the HR achieved. Patients with resting Wmas appear to b e at increased risk for perioperative events even without provokable ischemia.

关键词：负荷超声心动图室壁运动异常多巴酚丁胺心脏事件非心脏手术阴性患者预测价值心肌缺血患者 β受体阻滞剂次极

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