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热应激蛋白在运输应激的猪肾脏中的表达

农业生物技术学报 2001年第3期9卷 230-232页

作者：鲍恩东,Karim R.Sultan,B.nowak,J.Hartung 南京农业大学动物医学院 Department of the Science of Food of Animal Origin Faculty of　Veterinary Medicine University of Utrecht Department of Food Meat Hygiene and Technology Hannover School of　Veterinary Medicine Institute of Animal Hygiene and Animal W

利用Ｗｅｓｔｅｒｎ　ｂｌｏｔ技术，研究了长途运输实验猪肾脏中５种不同分子量的热应激蛋白ＨＳＰ７０　、ＨＳＰ７２、ＨＳＰ８６、ＨＳＰ９０和ＨＳＰ２７的表达。所有长途运输后应激猪和对照猪肾脏组织中均检测到了上述５种ＨＳＰ... 详细信息

利用Ｗｅｓｔｅｒｎ　ｂｌｏｔ技术，研究了长途运输实验猪肾脏中５种不同分子量的热应激蛋白ＨＳＰ７０　、ＨＳＰ７２、ＨＳＰ８６、ＨＳＰ９０和ＨＳＰ２７的表达。所有长途运输后应激猪和对照猪肾脏组织中均检测到了上述５种ＨＳＰ，对照猪肾脏组织中ＨＳＰ的表达说明ＨＳＰ具有独立于应激刺激应答以外的在细胞内行使正常生理功能的作用；６　ｈ的长途运输应激后，ＨＳＰ在肾脏组织中的表达量发生明显改变，但不同家族的ＨＳＰ的表达量显著不同，表现为ＨＳＰ７０家族的ＨＳＰ７０（Ｐ＜０．０１）、ＨＳＰ７２（Ｐ＞０．０５）和ＨＳＰ９０家族的ＨＳＰ８６（Ｐ＞０．０５）、ＨＳＰ９０（Ｐ＜０．０１）的表达明显下降，提示肾脏组织在长途运输中病理损伤的可能性。实验结果也显示ＨＳＰ２７的表达量略有升高（Ｐ＜０．０５），说明在应激反应中不同家族的ＨＳＰ可能有不同的生理作用。

关键词：猪运输应激应激蛋白肾脏

Possible involvement of group Ⅱ/Ⅲ metabotropic glutamate receptors in the mechanism of action of antidepressant drugs

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Possible involvement of group Ⅱ/Ⅲ metabotropic glutamate r...

上海与孟买的城市公共支出与城市贫困：基础设施、教育与公共卫生的财政预算支出

第十五届国际药理学大会

作者： Pilc Andrzej nowak Gabriel Palucha Agnieszka Wieronska Joanna Klak Kinga Branski Piotr Institute of Pharmacology Polish Acad.Sci.31-343 KrakowPoland. IF PAS KrakowPoland. IF PAS KrakowPoland. IF PAS KrakowPoland. IF PAS KrakowPoland. IF PAS KrakowPoland.

＜正＞ We investigated the effect of chronic （21 days） treatment with antidepressant drugs on group II/III mGlu receptors reactivity in rat brain. Chronic imipramine treatment reduced the ability of group II mGlu receptors agonist. 2R. 4R -APDC, to inhibit forskolin - stimulated cAMP formation in slices of rat cerebral cortex. Moreover,we observed the attenuation ofmGluR2/3 agonist （L - CCG -I） stimulated cAMP accumulation in the same preparation. Prolonged treatment with imipramine or citalopram did not changed the action of group III mGluR agonist ACPT - I on forskolin - stimulated cAMP accumulation. Binding studies have shown no influence of chronic treatment with antidepressants on the density （Bmax） or affinity （Kd） of [3H] - L Y341495 binding to mGluR2/3 receptors in the rat cerebral cortex or hippocampus. In behavioral studies we also investigated potential antidepressant - like effect of group II mGluRs antagonist. LY341495 as well as ACPT - I. We have found that both compounds produced a significant,dose- dependent decrease in the immobility time in mice or rats,suggesting,that modulation of group II/III mGlu receptors may produce an antidepressant- like effect.

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上海与孟买的城市公共支出与城市贫困：基础设施、教育与公共卫生...

作者：那思博（Spike Anthony nowak）南京大学

学位级别：硕士

本文的研究目的在于比较上海与孟买两个城市的某些公共支出和城市贫困情况。在西方国家或是在印度,学者、记者与政客经常把上海与孟买进行比较,但是这些研究只关注于这两座城市表面上的异同,而忽视这些表面上异同背后的根本原因。人们...

本文的研究目的在于比较上海与孟买两个城市的某些公共支出和城市贫困情况。在西方国家或是在印度,学者、记者与政客经常把上海与孟买进行比较,但是这些研究只关注于这两座城市表面上的异同,而忽视这些表面上异同背后的根本原因。人们很容易就能够看到的城市必要服务有交通基础设施、教育系统、电力基础设施与医疗卫生系统,都取决于其背后隐藏的城市公共支出的决策与财政能力。如果城市必要的公共支出不够,就将导致这个城市的公共服务求过于供,从而影响一个城市减贫的能力,正如在孟买与其他发展中国家的城市所见到的一样。本文把将会以上海与孟买的特定情况为例,来进一步说明广大发展中国家所面临的城市化问题,即城市财政问题与贫困问题。随着发展中国家的城市经济发展,通常其人口增长快于其财政资源、公共服务与基础设施的增长,因此导致城市领导人碰到很多难以取舍财政决策。不同的财政支出决策对市民的生活将产生不同的影响。以往的研究表明,提高某些公共支出,如教育支出、基础设施支出与医疗卫生支出,可以使贫困率降低。在上述阐述的较大背景中,本文将分析上海与孟买的教育、医疗卫生与基础设施支出,然后解释这两个城市的贫困状况。在分别分析上海与孟买的公共支出与贫困情况之后,本文将比较这两个城市的各项公共支出,来更好解释表面上的上海与孟买的社会情况与物质差别的异同。前人的研究只注重了比较上海与孟买的易于察觉的表面上的异同,但是本文是第一篇以深度分析研究上海与孟买城市公共支出的历史,来阐述这两个城市所形成的不同的城市模样与人民的生活模式。

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Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

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World Journal of Gastroenterology 2009年第9期15卷 1079-1084页

作者： Piet Habbel Karsten H Weylandt Katja Lichopoj Johannes nowak Martin Purschke Jing-Dong Wang Cheng-Wei He Daniel C Baumgart Jing X Kang Department of Medicine Massachusetts General Hospital and Harvard Medical School Boston MA 02114 United States Department of Medicine Division of Gastroenterology and Hepatology Charité Medical Center-Virchow-Hospital Medical School of the Humboldt-University of Berlin 13344 Berlin Germany

AIM： To investigate the impact of arachidonic acid （AA） and docosahexaenoic acid （DHA） and their combination on colon cancer cell growth. METHODS： The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death, flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 （COX-2）, p21 and bcl-2 in ceils incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 （PGE2） generation in the presence of AA and DHA was measured using a PGE2- ELISA. RESULTS： AA increased cell growth, whereas DHA reduced viability of LS 174T cells in a time- and dosedependent manner. Furthermore, DHA down- regulated mRNA of bcl-2 and up-regulated p21. Interestingly, DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation. DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation, DHA directly reduced PGE2-induced cell proliferation in a dosedependent manner. CONCLUSION： These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2.

关键词： Colorectal carcinoma Colon cancer Omega-3 Omega-6 Polyunsaturated fatty acids Arachidonic acid Docosahexaenoic acid ProstaglandinE2 Cyclooxygenase-2 Apoptosis

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用加脂剂提高染色革耐晒度的可能性

皮革化工 1983年第1期 48-53页

作者： R·nowak Krefeld 杨立贵

染色革的耐晒性是未整饰或稍加整饰过革的决定性的质量标准。染色革的耐晒性除了染料的选择之外,所用助剂的种类、投量及加脂剂都有很大的影响,实际上仅以选择染料,往往是达不到理想的耐晒牢度。经研究表明:在加脂期间加入少量的烷基... 详细信息

染色革的耐晒性是未整饰或稍加整饰过革的决定性的质量标准。染色革的耐晒性除了染料的选择之外,所用助剂的种类、投量及加脂剂都有很大的影响,实际上仅以选择染料,往往是达不到理想的耐晒牢度。经研究表明:在加脂期间加入少量的烷基磺酰氨,可明显地提高染料的耐晒牢度。皮革染色主要用的是酸性染料、直接染料和金属铬合染料,也使用少量的碱性染料。

关键词：磺酰氯代烷动物油磺化加脂剂耐晒牢度染色性能烷基磺酸钠盐

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帕金森在肠胃？

新发现 2012年第7期 102-106页

作者： Mathieu nowak 王佩瑶(编译)

你有没有想过帕金森可能不是脑科疾病，而是病原体在肠道引发的感染性疾病？在提出近10年后，这一猜测找到了越来越多的证据。如果只是一般的感染，那治愈帕金森病的希望就很大了！

关键词：感染性疾病帕金森病病原体

Antibacterial activity of nanostructured Ti-45S5 bioglass-Ag composite against Streptococcus mutans and Staphylococcus aureus

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Transactions of Nonferrous Metals Society of China 2016年第1期26卷 118-125页

作者： K.JURCZYK M.M.KUBICKA M.RATAJCZAK M.U.JURCZYK K.NIESPODZIANA D.M.nowak M.GAJECKA M.JURCZYK Department of Conservative Dentistry and Periodontology Poznan University of Medical Sciences Faculty of Pharmacy Department of Genetics and Pharmaceutical MicrobiologyPoznan University of Medical Sciences Division of Mother's and Child's Health Poznan University of Medical Sciences Institute of Materials Science and Engineering Poznan University of Technology Institute of Human Genetics Polish Academy of Sciences

Mechanical alloying and annealing at 1150 °C for 2 h under an argon atmosphere were used to prepare Ti-45S5 bioglass nanocomposites. Ti-45S5 bioglass material was chemically modified by silver. The antibacterial activity of Ti-10% 45S5 bioglass nanocomposite containing silver against Streptococcus mutans and Staphylococcus aureus was studied. Nanocomposites were characterized by X-ray diffraction, scanning electron microscopy equipped with an electron energy dispersive spectrometer and transmission electron microscopy to evaluate phase composition, crystal structure and grain size. In vitro bacterial adhesion study indicated a significantly reduced number of Streptococcus mutans and Staphylococcus aureus on the bulk nanostructured Ti-45S5 bioglass-Ag plate surface in comparison to that on microcrystalline Ti plate surface. Nanostructured Ti-based biomaterials can be considered to be the future generation of dental implants.

关键词： 45S5 bioglass antimicrobial activity silver titanium dental implant Streptococcus mutans Staphylococcus aureus

Functional recovery after peripheral nerve injury via sustained growth factor delivery from mineral-coated microparticles

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Neural Regeneration Research 2021年第5期16卷 871-877页

作者： Daniel J.Hellenbrand Clayton L.Haldeman Jae-Sung Lee Angela G.Gableman Elena K.Dai Stephen D.Ortmann Jerrod CGotchy Kierra K.Miller Adrianna M.Doucas Nicole C.nowak William L.Murphy Amgad S.Hanna Department of Neurological Surgery University of WisconsinMadisonWIUSA Department of Biomedical Engineering University of WisconsinMadisonWIUSA Department of Orthopedics and Rehabilitation University of Wisconsin Madison WIUSA

The gold standard for treating peripheral nerve injuries that have large nerve gaps where the nerves cannot be directly sutured back together because it creates tension on the nerve,is to incorporate an autologous nerve ***,even with the incorporation of a nerve graft,generally patients only regain a small portion of function in limbs affected by the ***,there has been some promising results using growth factors to induce more axon growth through the nerve graft,many of these previous therapies are limited in their ability to release growth factors in a sustained manner and tailor them to a desired time *** ideal drug delivery platform would deliver growth factors at therapeutic levels for enough time to grow axons the entire length of the nerve *** hypothesized that mineral coated microparticles(MCMs)would bind,stabilize and release biologically active glial cell-derived neurotrophic factor(GDNF)and nerve growth factor(NGF)in a sustained ***,the objective of this study was to test the ability of MCMs releasing growth factors at the distal end of a 10 mm sciatic nerve graft,to induce axon growth through the nerve graft and restore hind limb *** sciatic nerve grafting in Lewis rats,the hind limb function was tested weekly by measuring the angle of the ankle at toe lift-off while walking down a *** weeks after grafting,the grafts were harvested and myelinated axons were analyzed proximal to the graft,in the center of the graft,and distal to the *** physiological conditions in vitro,the MCMs delivered a burst release of NGF and GDNF for 3 days followed by a sustained release for at least 22 *** vivo,MCMs releasing NGF and GDNF at the distal end of sciatic nerve grafts resulted in significantly more myelinated axons extending distal to the graft when compared to rats that received nerve grafts without growth factor *** rats with nerve grafts incorporated with MCMs releasing NGF and G

关键词： autografts axon growth drug delivery glial cell-derived neurotrophic factor growth factors mineral coatings nerve grafting nerve growth factor

抗白细胞介素2受体单克隆抗体在肝移植中的应用

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中华器官移植杂志 2003年第3期24卷 185-187页

作者：葛旭鹏 Gunnar Soderdahl 吴国生 Grzegorz nowak Bo-Goran Ericzon 白求恩医科大学第一医院普外科长春130021 TransplantationDepartmentHuddingeHospital 第四军医大学附属西京医院胃肠外科

目的　探讨抗白细胞介素 2受体单克隆抗体 (抗IL 2R单克隆抗体 )的不同给药时间对肝移植后排斥反应的影响。方法　将 2 1例肝移植患者分为两组 ,均于手术当天及术后第 4d分别应用 2 0mg的抗IL 2R单克隆抗体 ,不同的是前 10例患者的首次... 详细信息

目的　探讨抗白细胞介素 2受体单克隆抗体 (抗IL 2R单克隆抗体 )的不同给药时间对肝移植后排斥反应的影响。方法　将 2 1例肝移植患者分为两组 ,均于手术当天及术后第 4d分别应用 2 0mg的抗IL 2R单克隆抗体 ,不同的是前 10例患者的首次剂量于移植肝恢复血流后给予 (术中组 ) ,而后 11例患者在移植术开始前 2h给予 (术前组 )。两个组术后均同时使用环孢素A和激素。术后随访 3个月。结果　经活检病理证实的急性排斥反应 ,术中组的发生率为 5 / 10 ( 5 0 % ) ,术前组为 3/ 11( 2 7% ) ;术中组还有 3例急性排斥反应未经病理证实 ,但抗排斥治疗有效 ,而术前组无类似病例 ,若综合考虑 ,则术前组急性排斥反应发生率 ( 3/ 11)明显低于术中组 ( 8/ 10 ,P <0 .0 5 )。在抗IL 2R单克隆抗体应用的过程中没有发现明显的不良反应。结论　术前 2h给予抗IL

关键词：肝移植术抗白细胞介素2受体单克隆抗体给药时间急性排斥反应