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芒果MiERF3基因的克隆及表达分析

热带作物学报 2024年

作者：窦雅琪王倩 Muhammad Muzammal aslam 邵远志李雯海南大学热带农林学院海南大学生命健康学院海南大学三亚南繁研究院

芒果(Mangifera indica L.)是海南省重要的热带水果之一，呼吸跃变型水果，乙烯在芒果后熟过程中扮演着至关重要的角色。研究指出，ERF转录因子是乙烯信号通路的关键下游成分，作为最大的转录因子家族之一，起到调节信号传导和生理反应... 详细信息

芒果(Mangifera indica L.)是海南省重要的热带水果之一，呼吸跃变型水果，乙烯在芒果后熟过程中扮演着至关重要的角色。研究指出，ERF转录因子是乙烯信号通路的关键下游成分，作为最大的转录因子家族之一，起到调节信号传导和生理反应的作用。为了探究ERF转录因子对采后芒果果皮色泽的影响，本研究以‘贵妃’芒果为研究对象，通过PCR克隆得到MiERF3基因，利用生物信息学方法分析MiERF3的基本理化特征、保守结构域、蛋白质结构、进化关系等；利用实时荧光定量PCR(qRT-PCR)技术分别对MiERF3基因在芒果不同组织部位和采后贮藏时期的特异性表达进行分析。结果可得，MiERF3长度为717 bp，编码238个氨基酸，蛋白分子式为C1130H1763N339O355S9，分子量为26.066 kDa，理论等电点为8.62，带30个正电残基，带27个负电残基，蛋白具有亲水性，结构不稳定；MiERF3蛋白不含信号肽，不含跨膜区域，磷酸化修饰以苏氨酸为主，包含一个AP2保守结构域，二级结构以61.67%的无规卷曲为主，还含有27.75%的α螺旋、3.96%的β折叠、6.61%的延长链，其三维结构模型包含有β折叠3个和α螺旋1个，符合AP2结构域的特征；多序列比对和motif分析结果表明MiERF3蛋白属于ERF亚家族成员；系统进化树分析结果表明与MiERF3蛋白亲缘关系最近的是同为漆树科植物的阿月浑子PvERF3-like；洋葱亚细胞定位及转录自激活检测结果显示MiERF3蛋白定位于细胞核且具有转录自激活活性；实时荧光定量分析表明，MiERF3基因的表达量在不同芒果部位表达情况不同，其中在芒果果实阳面果皮的表达量最高；且在芒果贮藏期间，其表达量有明显的先增后减的趋势，故而猜测MiERF3在果实成熟转色过程中起正调控作用。本研究为揭示ERF转录因子在芒果采后成熟衰老过程中的调控作用提供理论依据。

关键词：芒果 ERF 生物信息学分析表达分析

荔枝LcWRKY47基因的克隆、亚细胞定位、表达及延缓果实褐变的功能初探

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热带作物学报 2024年

作者：程南谱王鑫 Muhammad Muzammal aslam 邵远志李雯海南大学三亚南繁研究院海南大学热带农林学院海南大学食品科学与工程学院海南大学生命健康学院

荔枝（Litchi chinensis Sonn.）是亚热带地区重要的经济作物之一，但荔枝采后难贮存、易褐变，是目前保鲜过程中存在的最大问题之一。WRKY家族是植物中常见的一类转录因子，主要调控植物的逆境胁迫响应以及成熟衰老等生理过程。为了探... 详细信息

荔枝（Litchi chinensis Sonn.）是亚热带地区重要的经济作物之一，但荔枝采后难贮存、易褐变，是目前保鲜过程中存在的最大问题之一。WRKY家族是植物中常见的一类转录因子，主要调控植物的逆境胁迫响应以及成熟衰老等生理过程。为了探究WRKY对采后荔枝果实成熟衰老的影响，本研究以‘妃子笑’荔枝为试验对象，克隆出LcWRKY47基因，长度为1077 bp;LcWRKY47蛋白中含有WRKY保守结构域，二级结构以无规则卷曲为主，占比65.64%，三级结构主要为无规则卷曲和α螺旋。通过motif分析及多序列比对，发现LcWRKY47蛋白属于第Ⅱ类WRKY转录因子；系统进化分析树表明荔枝LcWRKY47与同为无患子科的龙眼DlWRKY47亲缘关系最近；亚细胞定位实验表明，LcWRKY47蛋白定位在细胞核中；RTqPCR分析表明，2 mmol/L草酸处理后LcWRKY47基因在荔枝果皮和果肉中的表达趋势相同，均先升后降，后期处理组表达量均显著高于对照组，推测草酸处理后该转录因子在荔枝果实成熟衰老过程中起正调控作用。过表达LcWRKY47基因发现，转基因果实中LcWRKY47的表达量显著高于对照果实，且转基因荔枝衰老褐变情况明显优于处理组。综上推测，LcWRKY47转录因子可能在荔枝衰老与褐变过程中起着一定的调节作用。

关键词：荔枝 WRKY转录因子基因克隆亚细胞定位表达分析

Point-of-care ultrasonography spotlight:Could venous excess ultrasound serve as a shared language for internists and intensivists?

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World Journal of Critical Care Medicine 2024年第2期13卷 126-136页

作者： Anosh aslam Khan Hasham Saeed Ibtehaj Ul Haque Ayman Iqbal Doantrang Du Abhilash Koratala Department of Internal Medicine Monmouth Medical CenterLong BranchNJ 07740United States Department of Internal Medicine Trinitas Regional Medical CenterElizabethNJ 07202United States Department of Anesthesiology Dr.Ruth K M Pfau Civil HospitalKarachi 74400Pakistan Department of Internal Medicine Dow Medical CollegeKarachi 74200Pakistan Division of Nephrology Medical College of WisconsinMilwaukeeWI 53226United States

Point-of-care ultrasonography(POCUS),particularly venous excess ultrasound(VExUS)is emerging as a valuable bedside tool to gain real-time hemodynamic *** modality,derived from hepatic vein,portal vein,and intrarenal vessel Doppler patterns,offers a scoring system for dynamic venous congestion *** an assessment can be crucial in effective management of patients with heart failure *** facilitates diagnosis,quantification of congestion,prognostication,and monitoring the efficacy of decongestive *** such,it can effectively help to manage cardiorenal syndromes in various clinical *** or eVExUS explores additional veins,potentially broadening its *** VExUS demonstrates promising outcomes,challenges persist,particularly in cases involving renal and liver parenchymal disease,arrhythmias,and situations of pressure and volume overload *** in utilizing spectral Doppler is pivotal for clinicians to effectively employ this ***,the integration of POCUS,especially advanced applications like VExUS,into routine clinical practice necessitates enhanced training across medical specialties.

关键词： Point-of-care ultrasonography Ultrasound Venous excess ultrasound Doppler Congestion Heart failure

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黑芝麻种质资源苗期耐渍性鉴定及评价

江西农业大学学报 2024年第1期46卷 13-24页

作者：万泽华华之梦王敏 Muhammad Talha aslam 沈庭海卢维娜何斌方圣吴自明江西农业大学作物生理生态与遗传育种教育部重点实验室江西南昌330045 浙江省绍兴市农业科学研究院浙江绍兴312035

【目的】渍害是影响黑芝麻生长的主要障碍因素之一,鉴定黑芝麻种质资源苗期耐渍性,确定可靠的评价指标,为耐渍黑芝麻品种的选育和栽培提供理论依据。【方法】以52份黑芝麻种质资源为试验材料,设置苗期渍水7 d处理,测定存活率、株高和根... 详细信息

【目的】渍害是影响黑芝麻生长的主要障碍因素之一,鉴定黑芝麻种质资源苗期耐渍性,确定可靠的评价指标,为耐渍黑芝麻品种的选育和栽培提供理论依据。【方法】以52份黑芝麻种质资源为试验材料,设置苗期渍水7 d处理,测定存活率、株高和根长等7个苗期指标,计算各指标的耐渍系数,采用主成分分析、隶属函数分析、聚类分析、相关性分析和逐步回归分析,将不同黑芝麻种质资源进行耐渍性综合评价及指标筛选。【结果】主成分分析法将7个指标简化为4个相互独立的综合指标,能够包含全部信息的79.25%;利用隶属函数法计算耐渍性综合评价值,并对其进行聚类分析,将52份黑芝麻品种划分为三大类,第一类为高度耐渍品种共5份,第二类为中度耐渍品种共39份,第三类为渍害敏感品种共8份。相关性分析表明株高、根长、地上部生物量和地下部生物量为综合评价值的主要影响因子,且各指标间也有不同程度的相关性。利用回归分析法建立芝麻苗期耐渍性预测模型,并从7个鉴定指标中筛选出株高、根长、地上部生物量和地下部生物量4个关键指标。【结论】都昌黑芝麻、鄱阳黑芝麻、多蒴黑芝麻、星子黑芝麻和17-50为高度耐渍品种。株高、根长、地上部生物量和地下部生物量可作为快速鉴定芝麻苗期的耐渍性的关键指标。

关键词：黑芝麻苗期耐渍性主成分分析聚类分析综合评价

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The Influence of Depression on Different Age and Gender Groups in Bahawalpur

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Journal of Clinical and Nursing Research 2024年第7期8卷 196-207页

作者： Mehran Khan Muhammad Shahbaz Tooba Waris Shoaib Yaseen Iqra Afzal Emaan Khadim Sawera Usman Muhammad Ismail Shah Sulaiman aslam Zafar Iqbal Bahawalpur College of Pharmacy BMDC Complex63100PunjabPakistan

In Bahawalpur,a cross-sectional study assessed depression prevalence across age and gender groups,involving 442 participants from diverse socioeconomic backgrounds and *** the PHQ9 questionnaire,84%were found to meet depression *** findings revealed a higher incidence in females(88%)than males(79%),with the greatest disparity among young adults,particularly young women,due to factors like academic pressure and financial *** children had the lowest depression rates(68%),possibly due to better *** individuals exhibited more severe depression,likely related to aging and domestic *** study’s findings highlight a significant variation in depression severity across different demographic groups,with an overall higher incidence in *** research underscores the necessity for targeted mental health resources and interventions tailored to the specific needs of each demographic *** also points to the importance of addressing academic and socioeconomic stressors to mitigate depression,particularly among young *** the study provides valuable insights,it relies on self-reported data,which may introduce ***,future research should include clinical assessments to validate these findings and ensure a more accurate representation of depression within the community.

关键词： Influence Depression Different age Gender groups Bahawalpur

Long-term outcomes of titanium-nitride-oxide coated stents and drug-eluting stents in acute coronary syndrome:A systematic review and meta-analysis

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World Journal of Cardiology 2024年第5期16卷 293-305页

作者： Muhammad Ahmed Ali Fahim Afia Salman Hira Anas Khan Syed Muhammad Hasan Muskan FatimaBhojani Sarah aslam Amna Zia Ul Haq Vishal Reddy Bejugam Beena Muntaha Nasir Wajiha Gul Abdul Moeed Abdelrahman S Abdalla Muhammad Majid Muhammad Sohaib Asghar Md Al Hasibuzzaman Department of Internal Medicine Dow University of Health SciencesKarachi 74200Pakistan Department of Internal Medicine North Central Bronx HospitalBronxNY 10467United States Department of Urology Center for Academic MedicineStanford University School of MedicinePalo AltoCA 33872United States Department of Cardiovascular Imaging Cleveland ClinicClevelandOH 44195United States Division of Nephrology and Hypertension Mayo Clinic RochesterRochesterMN 55905United States Institute of Nutrition and Food Science University of DhakaDhaka 1000Bangladesh

BACKGROUND In severe cases of coronary artery disease,percutaneous coronary intervention provide promising *** stent used could be a drug-eluting stent(DES)or a titanium-nitride-oxide coated stent(TiNOS).AIM To compare the 5-year effectiveness and safety of the two stent *** The following systematic review and meta-analysis was conducted in accordance with the preferred reporting items for systematic reviews and meta-analysis guidelines,and PubMed/MEDLINE,Scopus,and Cochrane Central were searched from inception till August *** outcomes were major adverse cardiac events(MACE),cardiac death,myocardial infarction(MI),cardiac death or MI,and ischemia-driven total lesion revascularization(ID-TLR).RESULTS Four randomized controlled trials(RCT),which analyzed a sum total of 3045 patients with acute coronary syndrome(ACS)after a median follow-up time of 5 years were *** statistically insignificant,an increase in the ID-TLR was observed in patients receiving TiNOSs vs *** addition,MI,cardiac death and MI,and definite stent thrombosis(DST)were significantly decreased in the TiNOS *** analysis revealed no significant results with meta-regression presenting non-ST elevated MI(NSTEMI)as a statistically significant covariate in the outcome of *** TiNOS was found to be superior to DES in terms of MI,cardiac death or MI,and DST outcomes,however,the effect of the two stent types on ID-TLR and MACE was not significant.A greater number of studies are required to establish an accurate comparison of patient outcomes in TiNOS and DES.

关键词： Stents Drug-eluting Major adverse cardiac events All-cause death Meta-analysis

Accretion onto the Magnetically Charged Regular Black Hole

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Chinese Physics Letters 2017年第7期34卷 38-40页

作者： M.Azam A.aslam Division of Science and Technology University of Education Lahore 54590 Pakistan

We investigate the accretion process for static spherically symmetric geometry, i.e., magnetically charged regular black hole with isotropic fluid. W＇e obtain generalized expressions for the velocity （u（r））, speed of sound （cs2）, energy density （ρ（r）） and accretion rate （M） at the critical point near the regular black hole during the accretion process. We also plot these physical parameters against fixed values of charge, mass and different values of equation of state parameter to study the process of accretion. We find that radial velocity and energy density of the fluid remain positive and negative as well as rate of change of mass is increased and decreased for dust, stiff, quintessence fluid and phantom-like fluid, respectively.

关键词： Accretion onto the Magnetically Charged Regular Black Hole

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Evaluation of the adsorption potential of titanium dioxide nanoparticles for arsenic removal

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Journal of Environmental Sciences 2009年第3期21卷 402-408页

作者： NABI Deedar aslam Irfan QAZI Ishtiaq A Institute of Environmental Science and Engineering National University of Sciences and Technology Rawalpindi 46000 Pakistan

The adsorption potential of titanium dioxide （TiO2） nanoparticles for removing arsenic from drinking water was evaluated. Pure and iron-doped TiO2 particles are synthesized via sol-gel method. The synthesized TiO2 nanoparticles were then immobilized on ordinary sand for adsorption studies. Adsorption isotherms were conducted on the synthesized nanoparticles as well as the sand coated with TiO2 nanoparticles under varying conditions of air and light, namely, the air-sunlight （A-SL）, air-light （AL）, air-dark （AD） and nitrogen-dark （ND）. X-ray diffraction （XRD） analysis showed that the pure and iron-doped TiO2 nanoparticles were in 100% anatase crystalline phase with crystai sizes of 108 and 65 nm, respectively. Adsorption of arsenic on the three adsorbents was non-linear that could be described by the Freundlich and Langmuir adsorption models. Iron doping enhanced the adsorption capacity of TiO2 nanoparticles by arresting the grain growth and making it visible light responsive resulting in a higher affinity for arsenic. Similarly, the arsenic removal by adsorption on the sand coated with TiO2 nanoparticles was the highest among the three types of sand used. In all cases, As（V） was adsorbed more compared with As（Ⅲ）. The solution pH appeared to be the most important factor in controlling the amount of arsenic adsorbed.

关键词： arsenic removal titanium dioxide nanoparticles adsorption isotherm immobilization of nanoparticles on sand

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B→0^+(1^+)+ missing energy in unparticle physics

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Chinese Physics C 2009年第5期33卷 332-339页

作者： M.Jamil aslam 吕才典 Institute of High Energy Physics CAS Department of Physics COMSATS Institute of Information Technology School of Physics and Technology Nanjing Normal University

We examine the effects of an unparticle U as a possible source of missing energy in the p-wave decays of a B meson. The dependence of the differential branching ratio on the K0^＊（K1） - meson＇s energy is discussed in the presence of scalar and vector unparticle operators and significant deviation from the standard model value is found after addition of these operators. Finally, we have shown the dependence of the branching ratio for the above-mentioned decays on the parameters of unparticle stuff like effective couplings, cutoff scale Au and the scale dimensions du.

关键词： unparticle missing energy B physics

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