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Human sperm quality and lipid content after migration into normal ovulatory human cervical mucus containing low numbers of leukocytes

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Asian Journal of Andrology 2009年第3期11卷 308-316页

作者： Nozha Chakroun-Feki Patrice Therond martine couturier Florence Eustache Gerard Limea Alain Legrand Pierre Jouannet Jacques Auger Laboratory of Reproductive Biology/CECOS （Center of Study and Conservation of human Eggs and Sperm） Cochin Hospital Rend Descartes University Paris 75014 France Laboratory of Clinical and Metabolic Biochemistry EA 3617 Rend Descartes University Paris 75270 Cedex 06 France INSERM U551 La Pitie Hospital 75651 Paris Cedex 13 France

The aim of this study was to investigate whether a relationship exists between the presence of low numbers of leukocytes in normal ovulatory cervical mucus and sperm quality and lipid content after migration. The percentages of live, motile and morphologically normal spermatozoa, movement parameters assessed by computer-aided sperm analysis （CASA）, and ionophore-induced acrosome reaction measured by flow cytometry were determined before and after migration. High-performance liquid chromatography with ultraviolet detection was used to measure the sperm lipid content, including the various diacyl subspecies. The number of leukocytes found in solubilized mucus samples was counted using a haemocytometric method. Overall, the presence of leukocytes in the cervical mucus samples did not significantly influence sperm motility and morphology, sperm kinematic parameters, or the sperm content in sphingomyelin or cholesterol. In contrast, after migration, the decrease in various sperm diacyls and the level of induced acrosome reaction was significantly less pronounced in mucus samples containing ≥10^4 leukocytes than in mucus samples with no or rare leukocytes whereas the level of induced acrosome reaction was higher. The present data suggest that the low level of leukocytes found in normal ovulatory cervical mucus could influence the process of sperm lipid remodelling/capacitation.

关键词： cervical mucus leukocyte lipid spermatozoa

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Amplification of Integrated Viral DNA and Flanking DNA Sequences Through the Activation of the Viral Replication Origin by E1/E2 Protein Expression in Cervical Carcinoma-derived Cells

Amplification of Integrated Viral DNA and Flanking DNA Seque...

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24th International Papillomavirus Conference and Clinical Workshop

作者： martine Peter Jerme couturier Ene Ustav Mart Ustav Xavier Sastre-Garau Institut Curie Institut Curie Dept.of Tumor Biology 26ruedUIm75248 Paris Cedex05 Institute of Technology Dept.of Biomedical Technology University of TartuNooruse 150411 TartuEstonia Institute of Technology Dept.of Biomedical Technology University of TartuNooruse 150411 TartuEstonia Institut Curie Dept.of Tumor Biology 26ruedUIm75248 Paris Cedex05

＜正＞In most cases of invasive cervical carcinoma,HPV DNA sequences are integrated into the host *** sequences, encompassing the URR and the E6/E7 ORFs,are amplified together with flanking cellular sequences Stretched amplicons analysis susggests that integration of HPV DNA occurs before amplification Recent data indicated that the re-expression of E1

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