检索结果-南通市图书馆

Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases

World Journal of Gastroenterology 2006年第41期12卷 6627-6633页

作者： Claudia Rubie Vilma Oliveira Frick Mathias Wagner Christina Weber Bianca Kruse Katja Kempf Jochen Knig Bettina Rau martin schilling Department of General- Visceral-Vascular-and Paediatric SurgeryUniversity of the SaarlandHomburg/Saar 66421Germany Institute of Pathology University of the SaarlandHomburg/Saar 66421Germany Institute of Medical Biometrics Epidemiologyand Medical InformaticsUniversity of the SaarlandHomburg/ Saar 66421Germany

AIM： To evaluate and compare the expression profiles of CXCL12 （SDF-1）, CCL19 （MIP-3β）, CCL20 （MIP-3a） and CCL21 （6Ckine, Exodus2） and their receptors on RNA and protein levels in hepatocellular carcinoma （HCC） versus colorectal liver metastases （CRLM） and to elucidate their impact on the carcinogenesis and progression of malignant liver diseases. METHODS： Chemokine expression was analyzed by RT-PCR and ELISA in 11 cases of HCC specimens and in 23 cases of CRLM and corresponding adjacent nontumorous liver tissues, respectively. Expressions of their receptors CXCR4, CCR6 and CCR7 were analyzed by RT- PCR and Western blot analysis in the same cases of HCC and CRLM. RESULTS： Significant up-regulation for CCL20/CCR6 was detected in both cancer types. Moreover, CCL20 demonstrated significant overexpression in CRLM in relation to the HCC tissues. Being significantly up-regulated only in CRLM, CXCR4 displayed an aberrant expression pattern with respect to the HCC tissues. CONCLUSION： Correlation of CXCR4 expression with CRLM suggests CXCR4 as a potential predictive factor for CRLM. High level expression of CCL20 and its receptor CCR6 in HCC and CRLM with marked up-regulation of CCL20 in CRLM in relation to HCC tissues indicates involvement of the CCL20/CCR6 ligand-receptor pair in the carcinogenesis and progression of hepatic malignancies.

关键词： Chemokines Chemokine receptors Gene expression Hepatocellular carcinoma Colorectal liver metastases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of preoperative FOLFOX chemotherapy on CCL20/CCR6 expression in colorectal liver metastases

引用

World Journal of Gastroenterology 2011年第26期17卷 3109-3116页

作者： Claudia Rubie Vilma Oliveira Frick Pirus Ghadjar Mathias Wagner Christoph Justinger Stefan Graeber Jens Sperling Otto Kollmar martin K schilling Department of General VisceralVascular and Paediatric SurgeryUniversity of the Saarland Department of Radiation Oncology InselspitalBern University Hospital and University of Bern Institute of Pathology University of the Saarland Institute of Medical Biometrics Epidemiologyand Medical Informatics(IMBEI)University of the Saarland

AIM： To evaluate the influence of preoperative FOLFOX chemotherapy on CCL20/CCR6 expression in liver metastases of stage IV colorectal cancer （CRC） patients.（/7 = 53） and in patients who did not receive FOLFOX chemotherapy prior to liver surgery （n = 29）. RESULTS： Of the 53 patients who received FOLFOX, time to liver surgery was n〈 1 mo in 14 patients, 〈 1 year in 22 patients and 〉 1 year in 17 patients, respectively. In addition, we investigated the proliferation rate of CRC cells in liver metastases in the different patient groups. Both CCL20 and CCR6 mRNA and protein ex- pression levels were significantly increased in patients who received preoperative FOLFOX chemotherapy ~〈 12 mo before liver surgery （P 〈 0.001） in comparison to patients who did not undergo FOLFOX treatment. Further, proliferation of CRLM cells as measured by Ki-67 was increased in patients who underwent FOLFOX treat- ment. CCL20 and CCR6 expression levels were significantly increased in CRLM patients who had undergone preoperative FOLFOX chemotherapy. CONCLUSION： This chemokine/receptor up-regulation could lead to increased proliferation/migration through an autocrine mechanism which might be used by surviving metastatic cells to escape cell death caused by FOLFOX.

关键词： FOLFOX chemotherapy CCL20/CCR6 expression Colorectal liver metastases Proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Correlation of IL-8 with induction, progression and metastatic potential of colorectal cancer

引用

World Journal of Gastroenterology 2007年第37期13卷 4996-5002页

作者： Claudia Rubie Vilma Oliveira Frick Sandra Pfeil Mathias Wagner Otto Kollmar Berit Kopp Stefan Grber Bettina M Rau martin K schilling Department of General Visceral Vascular and Paediatric Surgery University of the Saarland Homburg 66421 Germany Institute of Pathology University of the Saarland Homburg 66421 Germany Institute of Medical Biometrics Epidemiology and Medical Informatics University of the Saarland Homburg 66421 Germany

AIM： To investigate the expression profile of IL-8 in inflammatory and malignant colorectal diseases to evaluate its potential role in the regulation of colorectal cancer （CRC） and the development of colorectal liver metastases （CRLM）.METHODS： IL-8 expression was assessed by quantitative real-time PCR （Q-RT-PCR） and the enzyme-linked immunosorbent assay （ELISA） in resected specimens from patients with ulcerative colitis （UC, n = 6） colorectal adenomas （CRA, n = 8）, different stages of colorectal cancer （n = 48） as well as synchronous and metachronous CRLM along with their corresponding primary colorectal tumors （n = 16）.RESULTS： IL-8 mRNA and protein expression was significantly up-regulated in all pathological colorectal entities investigated compared with the corresponding neighboring tissues. However, in the CRC specimens IL-8 revealed a significantly more pronounced overexpression in relation to the CRA and UC tissues with an average 30-fold IL-8 protein up-regulation in the CRC specimens in comparison to the CRA tissues. Moreover, [L-8 expression revealed a close correlation with tumor grading. Most interestingly, IL-8 up-regulation was most enhanced in synchronous and metachronous CRLM, if compared with the corresponding primary CRC tissues. Herein, an up to 80-fold IL-8 overexpression in individual metachronous metastases compared to normal tumor neighbor tissues was found.CONCLUSION： Our results strongly suggest an association between IL-8 expression, induction and progression of colorectal carcinoma and the development of colorectal liver metastases.

关键词： Interleukin-8 Gene expression profiling RNA and protein expressioni Colorec cal cancer Colorectalliver metastases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IL-8在食管癌中表达对肿瘤血管形成及患者预后的影响

引用

华中科技大学学报（医学版） 2005年第4期34卷 451-453,457页

作者：苏铁芬 Bettina Tilton 袁永辉李娜萍 martin schilling 吴人亮华中科技大学同济医学院基础医学院病理系武汉430030 萨尔州大学洪堡医学院外科德国洪堡66424

目的研究白细胞介素8(IL8)在食管癌组织中的表达变化,以及此变化对肿瘤血管形成、患者预后的影响。方法利用实时逆转录聚合酶链反应(realtimeRT PCR)和免疫组织化学技术检测食管癌及正常组织中IL8mRNA和蛋白表达,并采用Ⅷ因子相关抗原(F... 详细信息

目的研究白细胞介素8(IL8)在食管癌组织中的表达变化,以及此变化对肿瘤血管形成、患者预后的影响。方法利用实时逆转录聚合酶链反应(realtimeRT PCR)和免疫组织化学技术检测食管癌及正常组织中IL8mRNA和蛋白表达,并采用Ⅷ因子相关抗原(FⅧRAg)检测微血管密度。结果食管癌组织中IL8mRNA及蛋白表达显著高于正常组织。IL8mRNA高表达与远处淋巴结转移、微血管高密度、生存时间短(<26个月)、早期复发(<16个月)有关(均P<0.05)。癌组织中微血管密度比周围正常组织高(P<0.01)。结论在食管癌中,IL8mRNA高表达的患者发生转移机率高,复发早,生存时间短;IL8可以作为一个判定食管癌预后的指标。

关键词：食管癌白细胞介素-8 微血管密度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Electronic properties of wurtzite GaAs： A correlated structural, optical, and theoretical analysis of the same polytypic GaAs nanowire

引用

Nano Research 2018年第9期11卷 4708-4721页

作者： Alexander Senichev Pierre Corfdir Oliver Brandt Manfred Ramsteiner Steffen Breuer Jorg schilling Lutz Geelhaar Peter Werner Max-Planck-lnstitut fur Mikrostrukturl)hysik Halle 06120 Germany PauI-Drude-lnstitut for Festkorperelektronik Berlin 10117 Germany Centre for Innovation Competence SiLi-nano Martin-Luther-Universitat Halle 06120 Germany Present address: Birck Nanotechnology Center Purdue University West Lafayette Indiana 47907 USA Present address: Fraunhofer HHI Berlin 10587 Germany Present address: ABB Corporate Research Baden-Dattwi15405 Switzerland

III-V compound semiconductor nanowires are generally characterized by the coexistence of zincblende and wurtzite structures. So far, this polytypism has impeded the determination of the electronic properties of the metastable wurtzite phase of GaAs, which thus remain highly controversial. In an effort to obtain new insights into this topic, we cross-correlate nanoscale spectral imaging by near-field scanning optical microscopy with a transmission electron microscopy analysis of the very same polytypic GaAs nanowire dispersed onto a Si wafer. Thus, spatially resolved photoluminescence spectra could be unambiguously assigned to nanowire segments whose structure is known with lattice-resolved accuracy. An emission energy of 1.528 eV was observed from extended zincblende segments, revealing that the dispersed nanowire was under uniaxial strain presumably due to interaction with its supporting substrate. These crucial information and the emission energy obtained for extended pure wurtzite segments were used to perform envelope function calculations of zincblende quantum disks in a wurtzite matrix as well as the inverse structure. In these calculations, we varied the fundamental bandgap, the electron mass, and the band offset between zincblende and wurtzite GaAs. From this multi-parameter comparison with the experimental data, we deduced that the bandgap between the F8 conduction and A valence band ranges from 1.532 to 1.539 eV in strain-free wurtzite GaAs, and estimated values of 1.507 to 1.514 eV for the F7-A bandgap.

关键词： nanowires crystal-phase quantumstructures wurtzite GaAs strain near-field scanning optical microscopy photoluminescence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于表面等离子体耦合的高密度金纳米线阵列

引用

物理学报 2012年第23期61卷 422-427页

作者：闫红丹 Peter Lemmens Johannes Ahrens martin Brring Sven Burger Winfried Daum Gerhard Lilienkamp Sandra Korte Aidin Lak Meinhard schilling Institute for Condensed Matter Physics Technische Universitt Carolo-WilhelminaBraunschweigGermany 38106 International Graduate School of Metrology Technische Universitat Carolo-WilhelminaBraunschweigGermany 38106 Institut für Anorganische und Analytische Chemie Technische Universitt Carolo-WilhelminaBraunschweigGermany 38106 Konrad-Zuse-Zentrum für Informationstechnik BerlinGermany 14195 Institute of Energy Research and Physical Technologies Technische Universitt ClausthalClausthal-ZellerfeldGermany 38678 Institute of Electrical Measurement and Fundamental Electrical Engineering Technische Universitt Carolo-WilhelminaBraunschweigGermany 38106

利用电化学沉积法在阳极氧化铝模板中制备了高长径比(20—100)金纳米线阵列,并用扫描俄歇电子显微镜对其结构进行了表征.紫外可见吸收光谱显示金纳米线的表面等离子共振包含横向吸收峰(transverse mode)和纵向吸收峰(longitudinal mode)... 详细信息

利用电化学沉积法在阳极氧化铝模板中制备了高长径比(20—100)金纳米线阵列,并用扫描俄歇电子显微镜对其结构进行了表征.紫外可见吸收光谱显示金纳米线的表面等离子共振包含横向吸收峰(transverse mode)和纵向吸收峰(longitudinal mode),具有很强的各向异性特征.纵向吸收峰的强度与入射光的偏振方向和入射角度有关,随着长径比的增加纵向吸收峰位置向高能方向移动.将纳米线之间的表面等离子体能量耦合与分子H聚合体的吸收光谱行为做了比较,认为相邻纳米线间的多重耦合使纵向吸收峰出现蓝移.利用有限元分析法模拟了电场在纳米线阵列和单根纳米线表面的不同分布.

关键词：表面等离子体共振金纳米线阵列阳极氧化铝模板

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：