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Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

World Journal of Gastroenterology 2009年第9期15卷 1079-1084页

作者： Piet Habbel Karsten H Weylandt Katja Lichopoj Johannes Nowak martin purschke Jing-Dong Wang Cheng-Wei He Daniel C Baumgart Jing X Kang Department of Medicine Massachusetts General Hospital and Harvard Medical School Boston MA 02114 United States Department of Medicine Division of Gastroenterology and Hepatology Charité Medical Center-Virchow-Hospital Medical School of the Humboldt-University of Berlin 13344 Berlin Germany

AIM:To investigate the impact of arachidonic acid (AA) and docosahexaenoic acid (DHA) and their combination on colon cancer cell ***:The LS-174T colon cancer cell line was used to study the role of the prostaglandin precursor AA and the omega-3 polyunsaturated fatty acid DHA on cell growth. Cell viability was assessed in XTT assays. For analysis of cell cycle and cell death,flow cytometry and DAPI staining were applied. Expression of cyclooxygenase-2 (COX-2),p21 and bcl-2 in cells incubated with AA or DHA was examined by real-time RT-PCR. Prostaglandin E2 (PGE2) generation in the presence of AA and DHA was measured using a ***:AA increased cell growth,whereas DHAreduced viability of LS 174T cells in a time-and dose-dependent manner. Furthermore,DHA down-regulated mRNA of bcl-2 and up-regulated p21. Interestingly,DHA was able to suppress AA-induced cell proliferation and significantly lowered AA-derived PGE2 formation. DHA also down-regulated COX-2 expression. In addition to the effect on PGE2 formation,DHA directly reduced PGE2-induced cell proliferation in a dose-dependent manner. CONCLUSION:These results suggest that DHA can inhibit the pro-proliferative effect of abundant AA or PGE2.

关键词：二十二碳六烯酸人结肠癌细胞花生四烯酸诱导扫描仪 LS 酶联免疫吸附试验前列腺素E2 酸抑制

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No plant functional diversity effects on foliar fungal pathogens in experimental tree communities

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Fungal Diversity 2014年第3期66卷 139-151页

作者： Lydia Hantsch Uwe Braun Josephine Haase Oliver purschke Michael Scherer-Lorenzen Helge Bruelheide Institute of Biology Geobotany and Botanical GardenMartin Luther University Halle WittenbergAm Kirchtor 106108 Halle(Saale)Germany Department of Environmental Systems Science ETH ZurichEcosystem ManagementInstitute of Terrestrial EcosystemsUniversitaetsstr.168092 ZurichSwitzerland German Centre for Integrative Biodiversity Research(iDiv)Halle-Jena-Leipzig Deutscher Platz 5e04103 LeipzigGermany Faculty of Biology GeobotanyAlberts-Ludwigs University of FreiburgSchaenzlestr.179104 FreiburgGermany

Foliar fungal pathogens affect forest ecosystem processes by exerting highly species-specific impacts on growth and survival of *** many ecosystem processes in forests depend on functional diversity of specific tree species,a close relationship is expected between this and foliar fungal pathogen *** for such a relationship in the German tree diversity experiment BIOTREE(Bechstedt),we hypothesized that pathogen richness and pathogen load decline with increasing functional diversity of tree *** macro-and microscopic analyses,we assessed pathogen richness and load on 16 tree species in plots that,although differing in functional diversity,had the same tree species *** found no effects of functional diversity on pathogen richness or ***,we encountered strong species identity effects in plot species composition,as susceptible tree species contributed positively to each community’s pathogen richness and ***,testing for effects of particular leaf traits and geographical range size of host species revealed a significant effect of total leaf phenolics,which was unexpected as pathogen richness increased with increasing content in *** study showed that at the community level,host species’identity was more important for foliar fungal pathogen richness and load than the functional diversity of host *** positive relationship between pathogen richness and phenolics in leaves,along with the finding that pathogen richness is very much conserved in tree species,point to an evolutionary arms race between hosts and fungi resulting from fungi increasing their capacity to infect tree leaves and trees boosting their defences.

关键词： Biodiversity-ecosystem functioning BIOTREE experiment Host defence traits Phylogenetic pattern Polyphenolics Tree species identity effects

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