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Asterohyptis stellulata: Phytochemistry and wound healing activity

Food Bioscience 2022年第6期50卷 2067-2076页

作者： Nallely Álvarez-Santos Edgar Antonio Estrella-Parra José del Carmen Benítez-Flores Rocío Serrano-Parrales Tomás Ernesto Villamar-Duque martha angelica santiago-santiago María del Rosario González-Valle José Guillermo Avila-Acevedo Ana María García-Bores Phytochemistry Laboratory UBIPROSuperior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Postgraduate Biological Sciences Postgraduate Studies UnitNational Autonomous University of Mexico (UNAM)CoyoacanMexico City04510Mexico Histology Laboratory 1 UMFSuperior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Natural Products Bioactivity Laboratory UBIPROSuperior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Bioterium Superior Studies Faculty (FES)-IztacalaNational Autonomous University of Mexico (UNAM)Tlalnepantla de BazMexico State54090Mexico Plant Physiology Laboratory UBIPROFES-IztacalaUNAMTlalnepantla de BazMexico State54090Mexico

Wound healing is a fast and efficient process. However, several factors can inhibit healing, causing scars and chronic wounds. This affects patients in their physical integrity, economic and emotional stability. Many plants that are used in wound healing treatment have antioxidant/anti-inflammatory activity through ROS regulation and antibacterial activity. Asterohyptis stellulata is used for wound treatment. Nonetheless, this activity has not been evaluated and its secondary metabolites are unknown. Therefore, the objective of this study was to analyze its phytochemical composition and evaluate the healing activity of A. stellulata. For the identification of secondary metabolites, the following were carried out: phytochemical screening, quantification of total phenols, and HPLC/MS analysis. The antibacterial and antioxidant activities were also determined. Next, to evaluate the healing activity of topical application of A. stellulata, the extract was mixed with the Beeler base to form an emulsion and an irritability test was performed. The emulsion was evaluated on incisional wounds of CD1 et/et mice, with tests of tensile strength, total re-epithelialization time, wound closure speed, and histological analysis. The phytochemical analysis showed that A. stellulata has phenolic compounds, two quercetin derivatives and a glycoside rosmarinic acid as main compounds. The extract exhibited medium antibacterial and antioxidant activities. A. stellulata emulsion was non-irritating, promoted closure speed of wound (1.479 ± 0.043 mm/day), increased tensile strength (980 ± 89.44 g), and histological analysis showed it promoted mice skin regeneration, compared to the controls (Recoveron® and Beeler base). This study confirms the traditional use of the A. stellulata.

关键词： Asterohyptis stellulata Phytochemistry Skin regeneration Wound healing

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Use of an Electroacoustic Device and Bone Densitometry in the Diagnosis of Osteoporosis: Utility, Validation, and Comparison

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Open Journal of Applied Sciences 2020年第5期10卷 210-218页

作者： Teodoro Cordova-Fraga martha Alicia Hernandez-Gonzalez angelica Hernandez-Rayas Daniel Alberto Ponce-Covarrubias Departamento de Ingeniería Física-DCI Universidad de Guanajuato campus León Loma del Bosque N. 103 Lomas del Campestre León GTO Mexico IMSS Unidad Médica Alta Especialidadclínica T1 Bajío. Blvd. Adolfo López Mateos S/N Los Paraísos León GTO Mexico

According to the World Health Organization (WHO), men and women older than 50 years present osteoporosis with reduced bone mass, this is one of the major impact morbidities causes in the world. It may be present in the hip, spine, and wrist. The gold standard technique for osteoporosis diagnosis is the Bone Densitometry (DXA). The evaluation of an electroacoustic device is as an alternative procedure for the validation and diagnosis of osteoporosis. Diagnosis of osteoporosis severity was carried out in 49 females by using the registers of the DEXA’s T-Score values. These were statistically compared with the measurements performed using the electroacoustic device. Non-Significative difference between measurements was found with ANOVA and Chi-square tests and the area for the ROC curve on electroacoustic devices was 0.551. The Bland Altman suggests an excellent concordance between both techniques. This is a non-invasive method that has a proper match with the gold standard. The study suggests that procedure measurements with the electroacoustic device could be implemented as an alternative clinical practice for osteoporosis diagnosis.

关键词： Electroacoustic Device New Diagnostic Tests Densitometry

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