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Efficacy and long-term evaluation of intramyocardial injection of autologous CD34-enriched PBMSC in old myocardial infarction

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World Journal of Cardiovascular Diseases 2012年第4期2卷 283-290页

作者： Jose Luis Aceves Abel Archundia Araceli Paez Rafael Vilchis Elvira Varela Emma Rodriguez Guillermo Diaz Lourdes Flores-Luna martha alvarado Manuel Lopez Hernandez Luis Felipe Montano Felipe Masso Department of Cardiovascular Surgery National Medic Center 20 de Noviembre ISSSTEMexico CityMexico Department of Cellular Physiology National Institute of Cardiology“Ignacio Chavez”Mexico CityMexico Center of Population Health Research National Institute of Public HealthMexico CityMexico Department of Hematology National Medic Center 20 de Noviembre ISSSTEMexico CityMexico Immunobiology Laboratory Department of Cell and Tissue BiologySchool of Medicine UNAMMexico CityMexico

Aims: We have shown that autologous transplant of CD34+-enriched peripheral-blood mononuclear cells (PBMSC) could restore depressed myocardial function, and sustain adequate myocardial function 12 months after surgery in patients with old (>one year-old) myocardial infarction. Our aim is to report the long-term morbidity and mortality efficacy of this procedure. Methods and results: Seventy patients with an old anteroseptal myocardial infarction were followed for 2 to 7 years, 35 had a revascularization procedure and received an intra-myocardial injection of autologous CD34+-enriched PBMSC (8 × 108 mononuclear cells/ml including 3 × 107 CD34+ cells/ml)(Group A). Group B patients only had the revascularization. Abnormal pre-surgical values of LVEF (33.2% ± 4.8%), LVDV (178 ± 13.7 ml), LVSV (120 ± 16 m), LVDD (58.9 ± 3.84 mm), E and A waves without contractility in infarction area in group A patients improved to approximate normal values (50% ± 3% for LVEF;90 ± 9.3 ml for LVDV;80 ± 9.9 ml for LVSV;55.3 ± 3 mm for LVDD;5.2 ± 0.5 cm/s for E wave and 4.18 ± 0.3 cm/s for A wave) 1 year after the procedure and have remained unaltered for all the follow-up period. All the patients remain alive. Only seven patients have been readmitted to the hospital for non-myocardial related events. Group B only 11 patients continued alive to 5 years after surgery and LEVF never increased more than 6%, all of them with many hospitalizations (n ≥ 10) by heart failure events. Conclusion: Intramyocardial injection of CD34+ highly enriched PBSC represent an encouraging alternative for patients with severely scarred and dysfunctional myocardium.

关键词： Stem Cells Myocardial Infarction CD34+ Cells Revascularization Heart Failure Ventricular Function

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CXCR4+ and SDF-1+ Bone Marrow Cells Are Mobilized into the Blood Stream in Acute Myocardial Infarction and Acute Ischemia

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World Journal of Cardiovascular Diseases 2014年第7期4卷 361-367页

作者： Jose Luis Aceves Rafael Vilchis Maria Antonieta Medina Monserrat Borja Silvia Cortes Guillermo Diaz Armando Castro Alexis Gomez Jose JParra martha alvarado Manuel Lopez Hernandez Virna Poveda Felipe Masso Luis FMontano Division de Cardiocirugia Centro Medico Nacional 20 de Noviembre ISSSTEMexico DFMexico Departamento de Hematologia Centro Medico Nacional 20 de Noviembre ISSSTEMexico DFMexico Laboratorio de Hormonas Centro Medico Nacional 20 de Noviembre ISSSTEMexico DFMexico Departamento de Inmunobiologia Biologia Celular y Tisular Facultad de Medicina UNAMMexico DFMexico

Cell therapy has shown beneficial effects on ventricular function and tissue regeneration in patients with acute and chronic myocardial infarction, although with diverse grades of variability in the results, possibly by proportion, subtype and cell cycle status. Objective: Identify and phenotypically characterize, via CXCR4 and SDF-1 expression, the bone marrow cell subpopulations that are mobilized into the bloodstream in patients with Acute Myocardial Infarction (AMI) and Acute Ischemia (AI) such as acute angina and Chronic Ischemia (CI) such as chronic stable angina, and also determine the cell cycle status of these cells. Method: Patients with AMI and AI were recruited in the ICCU, and patients with CI in the departments of cardiology and cardiovascular surgery. The quantification of cellular subpopulations was made by cytofluorometry with a FACS caliburcyto fluorometry (Becton Dickinson) with specific FITC-labeled anti human monoclonal antibodies against CD34, CD133, CD117, CD48, CXCR4, SDF-1 and Ki67 (Becton Dickinson). Serum concentration of IL-6 and IL-8 were determined by a sequential solid phase chemiluminescent assay performed in a SIEMENS IMMULITE 1000 Analyzer. Statistical analysis was made with the SPSS version 20.0 for Windows. A p value 3/ml) than that in AI (9.2 ± 1.3 × 103/ml) and CI (6.6 ± 1.1 × 103/ml) patients (p p = 0.22 to 0.39), but interestingly in AMI and AI patients, cells were CXCR4+ in almost half of these mobilized cells, although the proportion was significantly higher in AMI patients (46.8% ± 7.1% to 55.7% ± 6.3% vs 23% ± 1.6% to 28.4% ± 2.1%, p = 0.03 to 0.05). A similar behavior was observed with the Ki67 antibody (29.9% ± 2.1% to 36.1% ± 6.3% vs 10% ± 1.2% to 24% ± 1.1%, p = 0.001 to 0.05). Bivariate analysis of the results showed a significant correlation of the cell proportion in AMI but not in AI and CI patients (p = 0.001 to 0.05;0.12 to 0.87 and 0.17 to 0.92 respectively). The amount of myocardial tissue infarcted did not show

关键词： Stem Cells CXCR4:SDF-1 Axis Cardiac Repair Acute and Chronic Ischemia

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Male age and strain affect ejaculate quality in the Mexican fruit fly

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Insect Science 2018年第4期25卷 703-711页

作者： Mariana Herrera-Cruz Solana Abraham Nicolas NuSez-Beverido Norma Flores-Estevez martha Reyes-Hernandez Mayvi alvarado Diana P6rez-Staples Catedra CONACYT- Facultad de Medicina y Cirugia Universidad Autonoma 'Benito Juarez' de Oaxaca Oaxaca Mexico Laboratorio de Investigaciones Ecoetologicas de Moscas de la Fruta y sus Enemigos Naturales (LIEMEN) PROIMI Tucuman Argentina CONICET Argentina INBIO TECA Universidad Veracruzana Xalapa Veracruz Mexico Instituto de Neuroetologia Universidad Veracruzana Xalapa Veracruz Mexico

Aging in all organisms is inevitable. Male age can have profound effects on mating success and female reproduction, yet relatively little is known on the effects of male age on different components of the ejaculate. Furthermore, in mass-reared insects used for the Sterile Insect Technique, there are often behavioral differences between mass-reared and wild males, while differences in the ejaculate have been less studied. The ejaculate in insects is composed mainly of sperm and accessory gland proteins. Here, we studied how male age and strain affected （i） protein quantity of testes and accessory glands, （ii） the biological activity of accessory gland products injected into females, （iii） sperm viability, and （iv） sperm quantity stored by females in wild and mass-reared Anastrepha ludens （Diptera： Tephritidae）. We found lower protein content in testes of old wild males and lower sperm viability in females mated with old wild males. Females stored more sperm when mated to young wild males than with young mass-reared males. Accessory gland injections of old or young males did not inhibit female remating. Knowledge of how male age affects different ejaculate components will aid our understanding on investment of the ejaculate and possible postcopulatory consequences on female behavior.

关键词： accessory glands age Anastrepha ludens mass-rearing sperm Tephritidae

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Efficacy and long-term evaluation of intramyocardial injection of autologous CD34-enriched PBMSC in old myocardial infarction

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World Journal of Cardiovascular Diseases（心血管病） 2012年第4期

作者： José Luis Aceves Abel Archundia Araceli Páez Rafael Vilchis Elvira Varela Emma Rodriguez Guillermo Diaz Lourdes Flores-Luna martha alvarado Manuel Lopez H. Luis Felipe Monta？o Felipe Masso

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The Pharmacologic Intensification of the Water Dissociation Process, or Human Photosynthesis, and Its Effect over the Recovery Mechanisms in Tissues Affected by Bloodshed of Diverse Etiology

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International Journal of Clinical Medicine 2011年第3期2卷 332-338页

作者： Arturo Solís Herrera María del Carmen Arias Esparza J. Jesús alvarado Esquivel Graciela Landín Miranda Ruth Isabel Solís Arias Paola Eugenia Solís Arias martha Patricia Solís Arias 不详

The photoreceptor layer of the human retina has several characteristics that are unique. Their energy requirements are the highest in the organism;in proportion, rods and cones require 10-fold the energy consumed by the cerebral cortex, 6-fold more than the cardiac muscle, and 3-fold more than the renal cortex. Astonishingly, the photoreceptor layer has no blood vessels at all. So, where is the energy to this tissue coming from? In this article we’ll describe the hitherto unknown explanation.

关键词： Melanin Hydrogen Oxygen Water Energy Human Photosynthesis

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