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Effect of Pseudomonas fluorescensand Burkholderia pyrrociniaon the Growth Improvement and Physiological Responses in Brachiaria brizantha

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American Journal of Plant Sciences 2018年第2期9卷 250-265页

作者： Monyck Jeane dos Santos Lopes Moacyr Bernardino Dias Filho Thomaz Henrique dos Reis Castro marta cristina corsi de filippi Gisele Barata da Silva Plant Protection Laboratory Institute of Agricultural Sciences Federal Rural University of Amazonia (UFRA) Belém Brazil Brazilian Agricultural Research Corporation (Embrapa) Belém Brazil Brazilian Agricultural Research Corporation (Embrapa) Goiania Brazil

The use of beneficial microorganisms in forage grasses is a potentially advantageous technique for a more sustainable pasture management by decreasing the need for chemical fertilization. Our aims were to determine the best method of microorganism inoculation on Brachiaria (Syn. Urochloa) brizantha cv. BRS Piata, compare the responses of inoculated plants of this forage grass with fertilized and unfertilized controls and examine its effect on some morphological, physiological and biochemical responses. On the first experiment, three inoculation methods were tested: in the seed, seed and soil, and soil, with Pseudomonas fluorescens (BRM-32111) and Burkholderia pyrrocinia (BRM-32113). In the second experiment, fertilized and unfertilized plants were either inoculated with BRM-32111, BRM-32113 and co-inoculated (BRM-32111 + BRM-32113). In a final experiment, B. brizantha was inoculated by soil drenching with BRM-32111, BRM-32113 and co-inoculated (BRM-32111 + BRM-32113), and compared to fertilized- and unfertilized-controls. The inoculation by soil drenching, at seedling stage, was more effective than inoculation only in the seed or both in the seed and by soil drenching. The fertilizer may have suppressed the beneficial bacterial effects on the growth of B. brizantha. P. fluorescens and B. pyrrocinia co-inoculated increased nitrate, protein, nitrogen concentration, Spad index (chlorophyll content), leaf area, number of tillers, net photosynthesis and total biomass production of B. brizantha plants. Our results point out to a potentially valuable source of practical information in the search of an eco-friendlier approach to increase pasture productivity.

关键词： Forage Grass Rhizobacteria Photosynthesis Nitrate Nitrogen

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Sexual dimorphism in the antitumor immune responses elicited by the combination of fasting and chemotherapy

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Cancer Communications 2024年第4期44卷 508-513页

作者： Andrés Pastor-Fernández Manuel Montero Gómez de las Heras Jose Ignacio Escrig-Larena marta Barradas cristina Pantoja Adrian Plaza Jose Luis Lopez-Aceituno Esther Durán Alejo Efeyan Maria Mittelbrunn Lola Martinez Pablo Jose Fernandez-Marcos Metabolic Syndrome Group(BIOPROMET) Madrid Institute for Advanced Studies(IMDEA)FoodE28049MadridSpain Molecular Biology Center Severo Ochoa(CBMSO) Spanish National Research Council(CSIC)Madrid Autonoma University(UAM)MadridSpain department of Basic Medical Sciences Institute of Applied Molecular MedicineSchool of MedicineUniversity Studies Center(CEU)-San Pablo UniversityMadridSpain Metabolism and Cell Signaling Laboratory Spanish National Cancer Research Centre(CNIO) MadridSpain 5Flow Cytometry UnitBiotechnology ProgrammeSpanish National Cancer Research Centre(CNIO)MadridSpain

Fasting reduces chemotherapy toxicity[1],enhances immunogenic tumor cell death[2,3]and increases CD8^(+)T cell infiltration in tumors,particularly when combined with chemotherapy[2,3]or immunotherapy[4].More-over,fast... 详细信息

关键词： chemotherapy fasting immune

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Epidemiology,therapy and outcome of hepatocellular carcinoma between 2010 and 2019 in Piedmont,Italy

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 761-772页

作者： Christian Bracco marta Gallarate Marco Badinella Martini Corrado Magnino Salvatore D'Agnano Roberta Canta Giulia Racca Remo Melchio cristina Serraino Valentina Polla Mattiot Giovanni Gollè Luigi Fenoglio department of Internal Medicine Santa Croce e Carle General HospitalCuneo 12100Italy department of Medical Sciences "City of Health and Science"University HospitalTorino 10100Italy

BACKGROUND Hepatocellular carcinoma(HCC)is the most common primary liver malignancy and the second leading cause of cancer deaths *** is often diagnosed at an advanced stage and therefore its prognosis remains poor with a low 5-year survival *** patients have increasingly complex and constantly changing characteristics,thus up-to-date and comprehensive data are *** To analyze the epidemiology and main clinical characteristics of HCC patients in a referral center hospital in the northwest of Italy between 2010 and *** In this retrospective study,we analyzed the clinical data of all consecutive patients with a new diagnosis of HCC recorded at"Santa Croce e Carle"Hospital in Cuneo(Italy)between 1 January 2010 and 31 december *** highlight possible changes in HCC patterns over the 10-year period,we split the population into two 5-year groups,according to the diagnosis period(2010-2014 and 2015-2019).RESULTS Of the 328 HCC patients who were included(M/F 255/73;mean age 68.9±11.3 years),154 in the first period,and 174 in the *** C virus infection was the most common HCC risk factor(41%,135 patients).The alcoholic etiology rate was 18%,the hepatitis B virus infection etiology was 5%,and the non-viral/non-alcoholic etiology rate was 22%.The Child-Pugh score distribution of the patients was:class A 75%,class B 21%and class C 4%.The average Mayo end-stage liver disease score was 10.6±3.7.A total of 55 patients(17%)were affected by portal vein thrombosis and 158(48%)by portal *** average nodule size of the HCC was 4.6±3.1 cm.A total of 204 patients(63%)had more than one nodulederwent ***,presence of ascites,BCLC stage and therapy w

关键词： Hepatocellular carcinoma Hepatitis virus Alcoholic hepatitis Non-alcoholic fatty liver disease Diagnosis Etiology Treatment

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Gsα^(R201C)and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells

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Bone Research 2022年第4期10卷 784-798页

作者： Biagio Palmisano Rossella Labella Samantha Donsante cristina Remoli Emanuela Spica Ilenia Coletta Giorgia Farinacci Michele dello Spedale Venti Isabella Saggio marta Serafini Pamela Gehron Robey Alessandro corsi Mara Riminucci department of Molecular Medicine Sapienza University of RomeRome 00161Italy Tettamanti Research Center Department of PediatricsUniversity of Milano Bicocca/Fondazione MBBMMonza 20900Italy department of Biology and Biotechnology Sapienza University of RomeRome 00185Italy Institute of Structural Biology and School of Biological Sciences Nanyang Technological University 639798 SingaporeSingapore CNR Institute of Molecular Biology and Pathology Piazzale Aldo Moro 5Rome 00185Italy Skeletal Biology Section National Institute of Dental and Craniofacial ResearchNational Institutes of HealthDepartment of Health and Human ServicesBethesdaMD 20892USA

The Gsα/cAMP signaling pathway mediates the effect of a variety of hormones and factors that regulate the homeostasis of the post-natal ***,the dysregulated activity of Gsαdue to gain-of-function mutations (R201C/R201H)results in severe architectural and functional derangements of the entire bone/bone marrow *** the consequences of gain-of-function mutations of Gsαhave been extensively investigated in osteoblasts and in bone marrow osteoprogenitor cells at various differentiation stages,their effect in adipogenically-committed bone marrow stromal cells has remained *** generated a mouse model with expression of Gsα^(R201C) driven by the Adiponectin(Adq)***-Gsα^(R201C) mice developed a complex combination of metaphyseal,diaphyseal and cortical bone *** the metaphysis,Gsα^(R201C) caused an early phase of bone resorption followed by bone *** bone formation was sustained by cells that were traced by Adq-Cre and eventually resulted in a high trabecular bone mass *** the diaphysis,Gsα^(R201C),in combination with estrogen,triggered the osteogenic activity of Adq-Cre-targeted perivascular bone marrow stromal cells leading to intramedullary bone ***,consistent with the previously unnoticed presence of Adq-Cre-marked pericytes in intraosseous blood vessels,Gsα^(R201C) caused the development of a lytic phenotype that affected both cortical(increased porosity)and trabecular(tunneling resorption)*** results provide the first evidence that the Adq-cell network in the skeleton not only regulates bone resorption but also contributes to bone formation,and that the Gsα/cAMP pathway is a major modulator of both functions.

关键词： estrogen sustained skeleton

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Occurrence of human pathogenic viruses in drinking water and in its sources:A review

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Journal of Environmental Sciences 2023年第10期132卷 145-161页

作者： Marco Panizzolo marta Gea Elisabetta Carraro Giorgio Gilli Silvia Bonetta cristina Pignata department of Public Health and Pediatrics University of TorinoPiazza Polonia 9410126TorinoItaly department of Life Sciences and Systems Biology University of Torinovia Accademia Albertina 1310123TorinoItaly

Since many waterborne diseases are caused by human pathogenic viruses,virus monitoring of drinking water(DW)and DW sources is crucial for public ***,the aim of this review was to describe the occurrence of human pathogenic viruses in DW and DW sources;the occurrence of two viruses proposed as novel indicators of human faecal contamination(Peppermild mottle virus and Tobacco mosaic virus)was also *** research was focused on articles that assessed viral occurrence using molecular methods in the surface water used for DW production(SW-D),groundwater used for DW production(GW-D),DW and bottled-DW(BW).A total of 1544 studies published in the last 10 years were analysed,and 79 were ultimately *** considering the detection methods,filtration is the most common concentration technique,while quantitative polymerase chain reaction is the most common quantification *** virus occurrence in SW-D,GW-D,and DW,high percentages of positive samples were reported for adenovirus,polyomavirus and Pepper mild mottle *** genomes were frequently detected in SW-D and rarely in GW-D,suggesting that GW-D may be a safe DW *** genomes were also detected in DW,posing a possible threat to human *** lowest percentages of positive samples were found in Europe,while the highest were found in Asia and South *** three articles assessed viral occurrence in *** review highlights the lack of method standardization and the need for legislation updates.

关键词： Drinking water Enteric virus Human health Microbial water quality Molecular methods Surface water

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The role of rhizobacteria in rice plants: Growth and mitigation of toxicity

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Journal of Integrative Agriculture 2018年第12期17卷 2636-2647页

作者： Marcela C F Rêgo Aline F Cardoso Thayná da C Ferreira marta C C de filippi Telma F V Batista Rafael G Viana Gisele B da Silva Plant Protection Laboratory Institute of Agrarian Sciences Federal Rural University of Amazon Phytopathology Laboratory Brazilian Enterprise for Agricultural Research-Rice and Beans

Allelopathic compounds reduce the growth and productivity of upland rice plants, especially in consecutive plantations. The rhizobacteria Pseudomonas fluorescens BRM-32111 and Burkholderia pyrrocinia BRM-32113 have been recorded as growth promoters in rice. This study was developed to understand the effect of the application of rhizobacteria on upland rice plants in consecutive plantations. Experiments were conducted in a completely randomized design with four replications of four treatments: rice seed inoculated with P. fluorescens BRM-32111, rice seed inoculated with B. pyrrocinia BRM-32113(both sown on soil with rice residue), non-inoculated plants sown on soil with rice residue(control with residue(WR)), and non-inoculated plants on soil with no residue(NR). Roots and seedling growth were adversely affected by allelopathic compounds in control WR plants. Plants inoculated with rhizobacteria P. fluorescens BRM-32111 or B. pyrrocinia BRM-32113 induced an increase of 88% in biomass, 3% in the leaf area, 40% in length, 67% in root biomass, 21% in chlorophyll a, 53% in chlorophyll(a+b), 50% in rate of carbon assimilation(A), 227% in A/rubisco carboxylation efficiency(Ci) and 63% in water use efficiency(WUE) compared to control WR plants. These results indicate that rhizobacteria P. fluorescens BRM-32111 and B. pyrrocinia BRM-32113 increase the tolerance of rice plants to stress from allelochemicals. There are possible practical agricultural applications of these results for mitigating the effects of environmental allelochemistry on upland rice.

关键词： allelopathy B.pyrrocinia P.fluorescens rhizobacteria rice

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An integrated approach to investigate climate-driven rockfall occurrence in high alpine slopes: the Bessanese glacial basin, Western Italian Alps

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Journal of Mountain Science 2020年第11期17卷 2591-2610页

作者： VIANI cristina CHIARLE marta PARANUNZIO Roberta MERLONE Andrea MUSACCHIO Chiara COPPA Graziano NIGRELLI Guido department of Earth Sciences University of Torino10125 TorinoItaly Research Institute for Geo-Hydrological Protection Italian National Research Council10135 TorinoItaly Institute of Atmospheric Sciences and Climate Italian National Research Council10133 TorinoItaly National Institute of Metrological Research 10135 TorinoItaly

Rockfalls are one of the most common instability processes in high *** represent a relevant issue,both for the risks they represent for(infra)structures and frequentation,and for their potential role as terrestrial indicators of climate *** study aims to contribute to the growing topic of the relationship between climate change and slope instability at the basin *** selected study area is the Bessanese glacial basin(Western Italian Alps)which,since 2016,has been specifically equipped,monitored and investigated for this *** order to provide a broader context for the interpretation of the recent rockfall events and associated climate conditions,a cross-temporal and integrated approach has been *** this purpose,geomorphological investigations(last 100 years),local climate(last 30 years)and near-surface rock/air temperatures analyses,have been carried *** research outcomes show that rockfalls occurred in two different geomorphological positions:on rock slopes in permafrost condition,facing from NW to NE and/or along the glacier margins,on rock slopes uncovered by the ice in the last *** thaw of the active layer and/or glacier debutressing can be deemed responsible for slope failure *** regard to timing,almost all dated rock falls occurred in *** the July events,initiation may have been caused by a combination of rapid snow melt and enhanced seasonal thaw of the active layer due to anomalous high temperatures,and *** events are,instead,associated with a significant positive temperature anomaly on the quarterly scale,and they can be ascribed to the rapid and/or in depth thaw of the permafrost active *** to our findings,we can expect that in the Bessanese glacierized basin,as in similar high mountain areas,climate change will cause an increase of slope instability in the *** fasten knowledge deepening,we highlight the need for a growth of a network of high elevation

关键词： Rockfalls Climate change Air and rock temperature Periglacial environment Western Italian Alps

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Distribution of DNA damage in the human sperm nucleus:implications of the architecture of the sperm head

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Asian Journal of Andrology 2020年第4期22卷 401-408页

作者： Silvia González-Rojo cristina Fernández-Díez marta Lombó María Paz Herráez department of Molecular Biology Faculty of BiologyUniversidad de LeónCampus de Vegazana s/n LeónLeón 24071Spain

The sperm nucleus is prone to sustain DNA damage before and after *** of the damage is not homogeneous,and the factors determining differential sensitivity among nuclear regions have not yet been *** sperm chromatin contains three structural domains,two of which are considered the most susceptible to DNA damage:the histone bound domain,harboring developmental related genes,and the domain associated with nuclear matrix *** a quantitative polymerase chain reaction(qPCR)approach,we analyzed the number of lesions in genes homeobox A3(HOXA3),homeobox B5(HOXB 5),sex-determining region Y(SRY)-box 2(SOX2),β-GLOBIN,rDNA 18S,and rDNA 28S in human sperm after ultraviolet irradiation(400μW cm−2,10 min),H2O2treatment(250 mmol l−1,20 min),and cryopreservation,which showed differential susceptibility to genetic *** vulnerability is dependent on the genotoxic agent and independent of the sperm nuclear proteins to which the chromatin is bound and of accessibility to the transcription *** of 8-hydroxy-2'-deoxyguanosine(8-OHdG)showed that the highest level of oxidation was observed after *** distribution of oxidative lesions also differed depending on the genotoxic agent.8-OHdG did not colocalize either with histone 3(H3)or with type IIα+βtopoisomerase(TOPO IIα+β)after H2O2treatment but matched perfectly with peroxiredoxin 6(PRDX6),which is involved in *** study reveals that the characteristics of the sperm head domains are responsible for access of the genotoxicants and cause differential degree of damage to nuclear areas,whereas chromatin packaging has a very limited *** histone-enriched genes analyzed cannot be used as biomarkers of oxidative DNA damage.

关键词： biomarkers DNA damage human sperm oxidative stress sperm chromatin sperm quality

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Long-term cross-sex hormone treatment is safe in ：ranssexual subjects

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Asian Journal of Andrology 2012年第6期14卷 813-814页

作者： Maria cristina Meriggiola marta Berra Center for Protection of Sexual Health Physiopathology of Reproduction and Gynecology University of Bologna and S Orsola-Malpighi Hospital Bologna 40138 Italy

The European Journal of Endocrinology recently published a paper （Asscheman et al.） presenting mortality data from more than a thousand transsexuals followed for a median time of 18 years and who had undergone prev...

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Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry

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BIOCELL 2013年第2期37卷 37-43页

作者： SOLANGE marta FRANZOI de MORAES THAIS ANDRÉIA BROGIO JACQUELINE NELISIS ZANONI MARIANA cristina VICENTE UMADA ZAPATER SIDNEY BARNABÉPERES LUZMARINA HERNANdeS Universidade Estadual de Maringá Avenida Colombo 5790Bloco H79sala 108 MaringáParanáBrazil87020900

Creatine is widely used by athletes as an ergogenic *** aim of this study was to evaluate the infl uence of creatine supplementation on the duodenum of rats submitted to physical *** number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were *** animals received a standard chow for 8 weeks,and the treated ones received the standard chow for 4 weeks and were later fed with the same chow but added with 2%*** were divided in groups:sedentary,sedentary supplemented with creatine,trained and trained supplemented with *** training consisted in treadmill running for 8 *** samples were either processed for whole mount preparations or for paraffi n embedding and hematoxylin-eosin staining for histological and morphometric studies of the mucosa,the muscular tunic and myenteric *** was observed that neither creatine nor physical training alone promoted alterations in muscular tunic thickness,villus height or crypts depth,however,a reduction in these parameters was observed when both were *** number of myenteric neurons was unchanged,but the neuronal cell body area was reduced in trained animals but not when training and creatine was associated,suggesting a neuroprotector role of this substance.

关键词： nutritional supplementation duodenum exercise

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