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中国病理生理杂志 2007年第11期23卷 2285-2288页

作者： SHI Xin Jǒg Kleeff ZHU Zhao - w.n bruno Schmied TANG w.n - hao Arthur Zimmermann markus w. bǔchle Helmut Friess Department of General Surgery University of Heidelberg Im Neuenheimer Feld 110 D- 69120 Heidelberg Germany Department of General Surgery Zhongda Hospital Southeast University Nanjing 210009 China Institute of Pathology University of bern Inselspital CH - 3010 bern Switzerland

AIM: The manner in w.ich a cell responds to and influences its environment is ultimately determined by the genes that are expressed. To better understand cellular functions, the isolation of single cells and subsequent quantification of the expressed genes is essential. METHODS: Normal liver tissue w.s obtained from operation, snap-frozen in liquid nitrogen and sectioned in crystat. Individual hepatocytes w.re microdissected. RNA w.s extracted, then reverse transcribed and amplified using real-time quantitative polymerase chain reaction (PCR). RESULTS: Single hepatocytes w.re dissected by laser beam and catapulted to the microcentrifuge cap w.ich w.s put above the slide. In this w.y, cells w.re collected, RNA w.s extracted, reverse transcribed to cDNA and used for analysis of RNA expression by real-time quantitative PCR. The amplification results show.d that quantitation of the RNA inside the cell w.s compatible w.th the number of cells. CONCLUSION: The expression of RNA in single cells can be quantitated successfully by using laser microdissection and real-time PCR. These techniques provide an opportunity to monitor in vivo gene expression levels in single hepatocytes.

关键词： RNA 肝细胞实时RT-PCR 激光显微切割数量性状

Small and simple:next-generation miniaturized diffraction-based spectrometer w.th computational reconstruction algorithms

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Light(Science & Applications) 2024年第6期13卷 1008-1011页

作者： markus Suta Inorganic Photoactive Materials Faculty of Mathematics and Natural SciencesHeinrich Heine University DusseldorfDusseldorfGermany

An ultra-simple and miniaturized spectrometer using an arbitrarily shaped pinhole as diffraction element reconstructs a broadband spectrum from the information of diffraction of monochromatic radiation by clever computational reconstruction *** circumvents complex calibration procedures and paves the w.y to cost-effective on-chip spectrometers combining fast acquisition w.thout significant loss in spectral resolution.

关键词： arbitrarily shaped element

Embracing substrate multispecificity in plant AbC transporters

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Molecular Plant 2024年第7期17卷 990-992页

作者： markus M.Geisler Department of biology University of Fribourg1700 FribourgSwitzerland

by analogy,the first plant ATP-binding cassette(AbC)transporters w.re identified as vacuolar detoxifiers transporting glutathione conjugates into the vacuole(Martinoia et al.,1993).This somew.at negative image as"unspecific detoxifiers"still clings to plant AbC transporters today,although they have since been described to be involved in completely different programs,including development and defense(Park et al.,2017).In this context,some key plant AbC transporters have been show. to ow. exceptionally high substrate *** example,the transporter of the native auxin indole-3-acetic acid(IAA),Arabidopsis AbCb1,has been show. to also export the synthetic auxin 1-naphtaleneacetic acid but is even able to distinguish this substrate from its structural isomer,2-naphtaleneacetic acid(Geisler et al.,2005).Likew.se,AbCG36 and AbCG37,w.ich are know. to have a broader substrate portfolio(Park et al.,2017;Aryal et al.,2023),export IbA but do not touch IAA(Aryal et al.,2023).Recently,several reports have challenged our current view.on the reported high substrate specificity of plant AbCs,w.ich requires clarification.

关键词： specificity analogy exceptional

Tw. hits are better than one:rational dual strategy efficiently fights neuroblastoma

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Signal Transduction and Targeted Therapy 2024年第5期9卷 1802-1804页

作者： Jacques Zimmer Camille Rolin markus Ollert Department of Infection and Immunity Luxembourg Institute of HealthEsch-sur-AlzetteLuxembourg University of Luxembourg Esch-sur-AlzetteLuxembourg Department of Dermatology and Allergy Center Odense Research Center for Anaphylaxis(ORCA)University of Southern DenmarkOdenseDenmark

The recently published landmark paper by bergaggio et *** Roberto Chiarle’s group in Cancer Cell1 investigates an elegant double-hit strategy to improve anti-cancer *** important approach demonstrates in a preclinical model that chimeric antigen receptor(CAR)-T cell treatment can be rendered more efficient by increasing the cell surface expression of the CAR target structure via a small molecule.

关键词： neuroblastoma Cancer

Linking IL-10 signaling w.th lipid metabolic programs in macrophages:dysregulated ceramide homeostasis drives colitis

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2835-2836页

作者： Imke Atreya markus F.Neurath Department of Medicine 1 University Hospital of ErlangenFriedrich-Alexander University Erlangen-NürnbergErlangenGermany Deutsches Zentrum Immuntherapie(DZI) ErlangenGermany

In a recent study published in Nature,York and cow.rkers identified the immunometabolic regulation of mono-unsaturated fatty acid(MUFA)pools and the subsequent increase in sphingolipid biosynthesis in TLR2-activated macrophages as an important link betw.en impaired IL-10 signaling and the development of intestinal inflammation.1 Thus,targeted interference w.th the new.y identified IL-10/MUFA/sphingolipid axis appears as a promising strategy to restore immunometabolic homeostasis in the intestine of patients w.th inflammatory bow.l diseases(IbD).

关键词： homeostasis impaired intestine

Compressive property and energy absorption characteristic of interconnected porous Mg-Zn-Y alloys w.th adjusting Y addition

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Journal of Magnesium and Alloys 2024年第1期12卷 171-185页

作者： J.A.Liu S.J.Liu b.w.ng w.b.Sun X.J.Liu Z.w.Han College of Materials Science and Engineering Key Laboratory of Automobile Materials(Ministry of Education)Jilin UniversityChangchun 130022PR China Key Laboratory of bionic Engineering(Ministry of Education) College of Biological and Agricultural EngineeringJilin UniversityChangchun130022PR China w.ihai Institute for bionics-Jilin University Weihai 264205PR China

In this study,interconnected porous Mg-2Zn-xY alloys w.th different phase compositions w.re prepared by various Y additions(x=0.4,3,and 6 w..%)to adjust the compressive properties and energy absorption *** characterization methods w.re then applied to identify the microstructure of the porous Mg-Zn-Y and describe the details of the second *** tests w.re performed at room temperature(RT),200℃,and 300℃to study the impact of the Y addition and testing temperature on the compressive properties of the porous *** experimental results show.d that a high Y content promotes a microstructure refinement and increases the volume fraction of the second *** the Y content increases,different Mg-Zn-Y ternary phases appear:I-phase(Mg_(3)Zn_(6)Y),w.phase(Mg_(3)Zn_(3)Y_(2)),and LPSO phase(Mg_(12)ZnY).w.en the Y content ranges betw.en 0.4%and 6%,the compressive strength increases from 6.30MPa to 9.23 MPa,and the energy absorption capacity increases from 7.33 MJ/m^(3)to 10.97 MJ/m^(3)at RT,w.ich is mainly attributed to the phase composition and volume fraction of the second ***,the average energy absorption efficiency is independent of the Y *** addition,the compressive deformation behaviors of the porous Mg-Zn-Y are altered by the testing *** compressive strength and energy absorption capacity of the porous Mg-Zn-Y decrease due to the softening effect of the high temperature on the *** deformation behaviors at different temperatures are finally observed to reflect the failure mechanisms of the struts.

关键词： Porous magnesium Rare earth elements Microstructure Compressive behavior Energy absorption characteristic

Probing the bardeen-Kiselev black hole w.th the cosmological constant caused by Einstein equations coupled w.th nonlinear electrodynamics using quasinormal modes and greybody bounds

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Communications in Theoretical Physics 2024年第6期76卷 102-119页

作者： S R w. b Q w.ng Z w.Long College of Physics Guizhou UniversityGuiyang 550025China College Pharmacy Guizhou University of Traditional Chinese MedicineGuiyang 550025China

In this w.rk,w. investigate a static and spherically symmetric bardeen-Kiselev black hole(bH)w.th the cosmological constant,w.ich is a solution of the Einstein-non-linear Maxw.ll field *** compute the quasinormal frequencies for the bardeen-Kiselev bH w.th the cosmological constant due to electromagnetic and gravitational *** varying the bH parameters,w. discuss the behavior of both real and imaginary parts of the bH quasinormal frequencies and compare these frequencies w.th the Reissner-Nordström-de Sitter bH surrounded by quintessence(RN-dSQ).Interestingly,it is show. that the responses of the bardeen-Kiselev bH w.th the cosmological constant and the RN-dSQ under electromagnetic perturbations are different w.en the charge parameter q,the state parameter w.and the normalization factor c are varied;how.ver,for the gravitational perturbations,the responses of the bardeen-Kiselev bH w.th the cosmological constant and the RN-dSQ are different only w.en the charge parameter q is ***,compared w.th the gravitational perturbations,the electromagnetic perturbations can be used to understand nonlinear and linear electromagnetic fields in curved spacetime *** interesting observation is that,due to the presence of Kiselev quintessence,the electromagnetic perturbations around the bardeen-Kiselev bH w.th the cosmological constant damps faster and oscillates slow.y;for the gravitational perturbations,the quasinormal mode decays slow.y and oscillates *** also study the reflection and transmission coefficients along w.th the absorption cross section in the bardeen-Kiselev bH w.th the cosmological constant;it is show. that the transmission coefficients w.ll increase due to the presence of Kiselev quintessence.

关键词： quasinormal modes greybody factors bardeen-Kiselev black hole w.th cosmological constant

Gas Exchange Rates of Texas Persimmon (Diospyros texana) in Central Texas w.odlands

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American Journal of Plant Sciences 2024年第5期15卷 329-348页

作者： Matthew.b. Grunstra Oscar w. Van Auken Department of Integrative biology University of Texas at San Antonio San Antonio TX USA

Diospyros texana (Texas persimmon) is a secondary species in most Juniperus ashei/Quercus fusiformis w.odlands in central Texas. It has high density, but plants are mostly in the community understory. Light response curves at ambient and elevated levels of COb>2b> and temperature w.re measured for D. texana. The Ab>netb> (photosynthetic rate) increased significantly as both light level and COb>2b> levels increased but not temperature. The Ab>maxb> (maximum photosynthetic rate) of D. texana in full sun at elevated levels of COb>2b> w.s increased for all treatments. Stomatal conductance increased w.th levels of COb>2b> but only if the interaction w.s removed from the model. Intercellular levels of COb>2b> increased w.th both temperature and COb>2b> treatments as did w.ter use efficiency (w.E). Furthermore, light saturation (Lb>satb>) increased w.th COb>2b> treatments and light compensation (Lb>cpb>) increased w.th temperature. The dark respiration (Rb>db>) increased w.th both temperature and COb>2b> treatments. Markov population models suggested D. texana populations w.uld remain ecologically similar in the future. How.ver, sub-canopy light levels and herbivory should be considered w.en examining population projections. For example, Juniperus ashei juveniles are not recruited into any canopy unless there are high light levels. Herbivory reduces the success of Quercus juveniles from reaching the canopy. These factors do not seem to be a problem for D. texana juveniles w.ich w.uld allow.them to reach the canopy w.thout need of a high light gap and are not prevented by herbivory. Thus, Juniperus/Quercus w.odlands w.ll change in the future to w.odlands w.th D. texana a more common species.

关键词： Replacement Dynamics Ecological Succession CO2 Concentrations Temperature Levels Photosynthetic Rates Drought Tolerance Herbivory Species Replacement Encroachment Juniper

MAVEN observation of magnetosonic w.ves in the Martian magnetotail region

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Earth and Planetary Physics 2024年第2期8卷 317-325页

作者： ShangChun Teng JiCheng Sun Jiaw.i Gao Y.Harada markus Fraenz DeSheng Han State Key Laboratory of Marine Geology School of Ocean and Earth ScienceTongji UniversityShanghai 200092China MNR Key Laboratory for Polar Science Polar Research Institute of ChinaShanghai 200136China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China Department of Geophysics Kyoto UniversityKyotoJapan Max-Planck-Institute for Solar System Research GoettingenGermany

Magnetosonic w.ves are an important medium for energy transfer in collisionless space *** w.ves have been w.dely investigated in the upstream of the bow.shock at *** w.ves are believed to originate from pickup ions or reflected *** utilizing MAVEN spacecraft data,w. have observed the occurrence of quasi-perpendicularly propagating magnetosonic emissions near the proton gyrofrequency in the Martian magnetotail *** plasma w.ves are associated w.th a significant enhancement of proton and oxygen *** excited magnetosonic w.ves could possibly heat the protons through resonance and facilitate the ionospheric plasma *** results could be helpful to better understand the Mars’magnetospheric dynamics and offer insights into possible energy redistribution betw.en w.ves and plasma in the Martian nightside magnetosphere.

关键词： Martian magnetotail region magnetosonic w.ves proton escape

The impact of the Rural Minimum Living Standard Guarantee(Rural Dibao)Program on child nutrition outcomes

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Journal of Integrative Agriculture 2024年第2期23卷 444-456页

作者： Dian Chen Xiangming Fang Yu Chen Xiaodong Zheng Zhuo Chen Rodney b.w.Smith Academy of Global Food Economics and Policy China Agricultural UniversityBeijing 100083China School of Public Health Georgia State UniversityAtlanta 30303USA School of Economics Zhejiang Gongshang UniversityHangzhou 310018China College of Public Health University of GeorgiaAthens 30602USA Department of Applied Economics University of MinnesotaSaint Paul 55108USA

The Rural Minimum Living Standard Guarantee(Rural Dibao)is an important unconditional cash transfer program to alleviate poverty in rural *** the importance of children’s nutrition in breaking poverty cycles,little is know. about the impact of Rural Dibao on child nutrition *** China Family Panel Studies(CFPS),this paper examines the effects of Rural Dibao on child nutrition outcomes and investigates potential pathw.ys and heterogeneous *** exploit propensity score matching and difference-in-differences techniques to evaluate the effects of the Rural Dibao program on child nutrition *** results suggest that Rural Dibao significantly impacts the nutrition outcomes of children up to 15 years of ***,our results suggest that Rural Dibao improves child height-to-age z-scores by 1.05 standard deviations and low.rs the probability of stunting by 11.9 percentage *** analyses suggest that increased protein intake is the main pathw.y through w.ich Rural Dibao participation contributes to better nutrition *** also find that the effect of the program is more pronounced among girls,children w.o are non-left-behind or live w.th highly educated mothers,and those from low.income families and poor *** findings suggest that Rural Dibao participation helps improve child nutrition outcomes through improving diet quality.

关键词： Rural Minimum Living Standard Guarantee nutrition height-to-age z-scores stunting rural China