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Fatty acids derived from oviposition systems guide female black soldier flies(Hermetia illucens)toward egg deposition sites

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Insect Science 2024年第4期31卷 1231-1248页

作者： Patrick klüber Emna Arous Jeanny Jerschow Marco Fraatz Daniel Bakonyi Martin Rühl Holger Zorn Department Bioresources Fraunhofer Institute for Molecular Biology and Applied EcologyGiessenGermany Institute of Food Chemistry and Food Biotechnology Justus Liebig University GiessenGiessenGermany

The black soldier fly,Hermetia illucens,comes with big promises for industrial purposes since its larvae feed polyphagously on a broad spectrum of organic ***,research focusing on adult flies is scarce,which is inconsistent with their reproductive relevance within the rearing *** particular,directed oviposition is a challenge in artificial ***,decomposing organic matter is commonly used as oviposition substrate,which has extensive potential for improvement in view of the lack of standardization and the risk of microbial ***,we identified three fatty acids and one fatty acid methyl ester derived from the surface of old oviposition sites and targeted to elucidate their effect on preference behavior and oviposition site selection using Y-olfactometry and prepared oviposition sites,*** to tetradecanoic acid attracted gravid females and stimulated oviposition most strongly,while decanoic acid demonstrated a repulsive *** kept in mixed-sex populations were attracted by tetradecanoic acid,resulting in a higher egg mass found in the compound box(3.0–11.4 fold),a≥2.3 fold reduction of nonspecifically deposited eggs,and the highest total egg ***,decanoic and dodecanoic acid caused females to lay a greater proportion of eggs nonspecifically outside both *** data suggest that fatty acids,especially tetradecanoic acid,are important cues for oviposition site selection in black soldier *** order to achieve a directed oviposition behavior,the role of further short-and long-chain fatty acids as attractants should be examined.

关键词： black soldier fly Hermetia illucens fatty acids insects as food and feed oviposition oviposition site selection tetradecanoic acid

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听觉躯体感觉双刺激模式通过减少大脑神经同步活动减轻豚鼠和患者的耳鸣程度

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听力学及言语疾病杂志 2018年第5期26卷 566-567页

作者：吴旭黄治物 marks kl Martel DT Wu C 上海交通大学医学院附属第九人民医院耳鼻咽喉头颈外科上海4200011

耳鸣是指患者没有受到外界声源或电刺激时,主观上耳内或颅内有声音感觉,是耳科较为常见的一种临床症状。据报道,在美国有15%的人口受到耳鸣的影响。耳鸣会严重地影响患者的生活质量,造成抑郁、焦虑、睡眠障碍和注意力不集中等困扰。然而... 详细信息

耳鸣是指患者没有受到外界声源或电刺激时,主观上耳内或颅内有声音感觉,是耳科较为常见的一种临床症状。据报道,在美国有15%的人口受到耳鸣的影响。耳鸣会严重地影响患者的生活质量,造成抑郁、焦虑、睡眠障碍和注意力不集中等困扰。然而,目前已有的耳鸣治疗方法仅能缓解患者的耳鸣反应,而不能完全治愈耳鸣。背侧耳蜗核是哺乳动物听觉通路上第一个多感官融合的位点,其主要的输出神经元细胞是梭形细胞。在耳鸣的中枢化机制中,耳鸣是由于神经活动节律失常引起的,其中神经自发活动和同步活动增加是其两个重要的病理生理特征。因此背侧耳蜗核被认为是耳鸣神经活动异常节律的起点。

关键词：同步活动大脑神经躯体感觉听觉通路耳鸣患者刺激模式豚鼠

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Clustering of voltage-gated ion channels as an evolutionary trigger of myelin formation

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Neural Regeneration Research 2024年第8期19卷 1631-1632页

作者： Henrike Ohm Simone Rey Christian klambt Institut fur Neuro-und Verhaltensbiologie MunsterGermany

Neurons carry apical dendrites that perceive information and a basal axon that transmits the computed information towards its to *** axon originates at the axon hillock which is followed by the axon initial ***,action potentials are initiated that are based on millisecond long openings of specific voltagegated sodium and potassium channels that are conserved in all parahoxozoa(Placozoa,Cnidaria,Bilateria)(Li et al.,2015).

关键词： initiated carry potassium

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Customized molecular tools to strengthen metabolic engineering of cyanobacteria

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Green Carbon 2024年第2期2卷 149-163页

作者： Stephan klähn Franz Opel Wolfgang R.Hess Department of Solar Materials Biotechnology Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany Genetics and Experimental Bioinformatics Albert-Ludwig University FreiburgFreiburg im Breisgau 79104Germany

Cyanobacteria are promising oxygenic phototrophs for the production of various *** their(photo)biotechnological exploitation,molecular tools are required,such as,for the introduction and expression of heterologous genes,or the modulation of enzyme activities or entire *** and strategies for the development of photosynthetic biomanufacturing technologies based on cyanobacteria have been extensively reviewed,as well as certain specialized aspects of their genetic ***,options for metabolic engineering of specific cyanobacterial cells are still less developed than those for other bacteria of biotechnological *** addition to the standard genetic toolbox for“classical”metabolic engineering,we emphasize certain aspects,including recently developed vector systems for the extrachromosomal maintenance of genes and approaches based on clustered regularly interspaced short palindromic repeats(CRISPR)*** highlight the development of custom molecular tools for specific strains or products,discuss the emerging use of small regulatory proteins that appear promising for advanced metabolic engineering approaches to promote specific product formation,and provide an overview of suitable online ***,we discuss the current trends in this field and indicate their potential,such as using suitable product sensors that enable systematic screening,and optimization approaches.

关键词： Metabolic engineering Cyanobacteria Synthetic plasmids Regulatory proteins Sensors

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Pathogenesis and clinical features of severe hepatitis E virus infection

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World Journal of Virology 2024年第2期13卷 19-33页

作者： László Orosz Károly Péter Sárvári Áron Dernovics András Rosztóczy klára Megyeri Department of Medical Microbiology University of SzegedSzeged 6720Csongrád-CsanádHungary Department of Internal Medicine Division of GastroenterologyUniversity of SzegedSzeged 6725Csongrád-CsanádHungary

The hepatitis E virus(HEV),a member of the Hepeviridae family,is a small,non-enveloped icosahedral virus divided into eight distinct genotypes(HEV-1 to HEV-8).Only genotypes 1 to 4 are known to cause diseases in *** 1 and 2 commonly spread via fecal-oral transmission,often through the consum-ption of contaminated *** 3 and 4 are known to infect pigs,deer,and wild boars,often transferring to humans through inadequately cooked *** hepatitis caused by HEV in healthy individuals is mostly asymptomatic or associated with minor symptoms,such as ***,in immunosup-pressed individuals,the disease can progress to chronic hepatitis and even escalate to *** pregnant women,an HEV infection can cause fulminant liver failure,with a potential mortality rate of 25%.Mortality rates also rise amongst cirrhotic patients when they contract an acute HEV infection,which can even trigger acute-on-chronic liver failure if layered onto pre-existing chronic liver *** the prevalence of HEV infection continues to rise worldwide,highlighting the particular risks associated with severe HEV infection is of major medical *** text offers a brief summary of the characteristics of hepatitis developed by patient groups at an elevated risk of severe HEV infection.

关键词： Hepatitis E virus Cirrhosis Acute-on-chronic liver failure Pregnancy Immune dysfunction Open reading frames 1-4

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Is there a potential of circulating miRNAs as biomarkers in rheumatic diseases?

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Genes & Diseases 2023年第4期10卷 1263-1278页

作者： klára Prajzlerová Ladislav Senolt Mária Filková Institute of Rheumatology Prague 12800Czech Republic Department of Rheumatology First Faculty of MedicineCharles UniversityPrague 12800Czech Republic

t MicroRNAs(miRNAs)are small non-coding single-stranded RNAs of about 22 nucle-otides in length that act as post-transcriptional regulators of gene *** on the complementarity between miRNA and target mRNA,cleavage,destabilization,or transla-tional suppression of mRNA occurs within the RISC(RNA-induced silencing complex).As gene expression regulators,miRNAs are involved in a variety of biological *** of miRNAs and their target genes contribute to the pathophysiology of many diseases,including autoimmune and inflammatory *** are also present extracellularly in their sta-ble form in body *** incorporation into membrane vesicles or protein complexes with Ago2,HDL,or nucleophosmin 1 protects them against ***-free miRNAs can be deliv-ered to another cell in vitro and maintain their functional ***,miRNAs can be considered mediators of intercellular *** remarkable stability of cell-free miRNAs and their accessibility in body fluid makes them potential diagnostic or prognostic bio-markers and potential therapeutic *** we provide an overview of the potential role of circulating miRNAs as biomarkers of disease activity,therapeutic response,or diagnosis in rheumatic *** circulating miRNAs reflect their involvement in the pathogenesis,while for plenty,their pathogenetic mechanisms remain to be *** miRNAs described as biomarkers were also shown to be of therapeutic potential,and some miRNAs arealready tested in clinical trials.

关键词： Biomarker Diagnosis miRNA Rheumatic diseases Therapy

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Recent advances on the synthesis and application of tetrahydro-γ-carbolines

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Chinese Chemical Letters 2023年第12期34卷 119-136页

作者： Haibo Mei klára Aradi Loránd Kiss Jianlin Han Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources Nanjing Forestry University Institute of Organic Chemistry Research Centre for Natural Sciences Stereochemistry Research Group

Tetrahydro-γ-carbolines(THγ Cs) constitute one of the most important subtypes of indole alkaloids. In addition to being substructures of natural products, these structural motifs and moieties can often be found in pharmaceuticals due to their diverse bioactivities such as antiviral, antibacterial, antifungal,antiparasitic, antitumor, anti-inflammatory, and neuropharmacological activities. Beyond the pharmacological and biological aspects of these scaffolds, they have considerable synthetic applications for the construction of further bioactive compounds, too. The aim of this review is to summarize recent developments in the synthesis of this compound class.

关键词： Tetrahydro-γ -carbolines Synthetic methods Bioactive molecuels Asymmetric synthesis Indole Polycyclic compounds

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Machine learning model for snow depth estimation using a multisensory ubiquitous platform

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Journal of Mountain Science 2022年第9期19卷 2506-2527页

作者： Sofeem NASIM Mourad OUSSALAH Björn klÖVE Ali Torabi HAGHIGHI Department of Computer Science and Engineering University of OuluOulu 90570Finland Water Energy and Environmental EngineeringUniversity of OuluOulu 90570Finland

Snow depth estimation is an important parameter that guides several hydrological applications and climate change *** advances in remote sensing technology and enhanced satellite observations,the estimation of snow depth at local scale still requires improved accuracy and *** advances in ubiquitous and wearable technology promote new prospects in tackling this *** this paper,a wearable IoT platform that exploits pressure and acoustic sensor readings to estimate and classify snow depth classes using some machine-learning models have been put ***,the results of Random Forest classifier showed an accuracy of 94%,indicating a promising alternative in snow depth measurement compared to in situ,LiDAR,or expensive large-scale wireless sensor network,which may foster the development of further affordable ecological monitoring systems based on cheap ubiquitous sensors.

关键词： Snow depth Wearable technology Machine learning Random forest classifier

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Speciation in nanosecond laser ablation of zinc in water

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Science China(Physics,Mechanics & Astronomy) 2022年第7期65卷 90-98页

作者： Stefan Reich Yannis klügl Anna Ziefuss RenéStreubel Jorg Gottlicher Anton Plech Institute for Photon Science and Synchrotron Radiation Karlsruhe Institute of TechnologyKarlsruhe D-76021Germany Department of Technical ChemistryⅠand Center for Nanointegration Duisburg-Essen University of Duisburg-EssenEssen D-45141Germany Fraunhofer Institute for High-Speed Dynamics Ernst-Mach-Institute(EMI)Freiburg 79104Germany

In situ experimental methods have been applied to resolve mass flow and chemical speciation in the pulsed laser ablation of zinc in water. The chemical speciation has been resolved by time-resolved μ-X-ray absorption spectroscopy and mapped onto the macroscopic mass flow during material ejection from the metallic target and bubble dynamics of evaporated water. Large particles and agglomerates have been detected via dark-field X-ray imaging with a Shack-Hartmann sensor. The characteristic of the dynamics is that the vapor bubble is nearly homogeneously filled with ablated material. This persists during bubble collapse,which means that the ablated particles are captured and retracted towards the target. Limited mass escape is indicated by the X-ray absorption signal. Importantly, the near-edge structure at the Zn-K;transition delivers information on the chemical state of the ejected material. It clearly confirms that oxidation is not present within the bubble phase and the following sub-millisecond time scale. The oxidation proceeds on Zn nanoparticles in suspension on a second to minute course. Within the first microseconds,a Zn atom phase is detected that resembles Zn vapor. The addition of either reductive NaBH;or oxidative HAuCl;to the water phase influences the quantity of the atom contribution moderately, but does not influence the initial atom phase. Such behavior must be understood in terms of the nanosecond pulse excitation. After ejected material and a plasma is formed within the pulse duration of 7 ns the laser is able to further heat the ejecta and transform it partly into vapor. Correspondingly, the coupling of energy into the ablation zone as followed by plasma intensity and bubble size follows a threshold behavior as a function of laser fluence, marking the onset of laser-plasma heating. The reaction conditions inside the bubble are probably reductive due to the concomitant formation of excess hydrogen.

关键词： laser ablation in liquids elemental speciation X-ray absorption spectroscopy oxidation zinc oxide nanoparticles

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Estimating Construction Material Indices with ARIMA and Optimized NARNETs

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Computers, Materials & Continua 2023年第1期74卷 113-129页

作者： Ümit Isıkdag Aycan Hepsag Sureyya Imre Bıyıklı DeryaÖz Gebrail Bekdas Zong Woo Geem Department of Informatics Mimar Sinan Fine Arts UniversityIstanbulTurkey Econometrics Department Istanbul UniversityIstanbulTurkey MIS Department Istanbul Gelisim UniversityIstanbulTurkey Computing HND Prog. Nisantasi UniversityIstanbulTurkey Department of Civil Engineering Istanbul University-CerrahpasaIstanbulTurkey College of IT Convergence Gachon UniversitySeongnam13120Korea

Construction Industry operates relying on various key economic *** of these indicators is material *** the other hand,cost is a key concern in all operations of the construction *** the uncertain conditions,reliable cost forecasts become an important source of *** cost is one of the key components of the overall cost of *** addition,cost overrun is a common problem in the construction industry,where nine out of ten construction projects face cost *** order to carry out a successful cost management strategy and prevent cost overruns,it is very important to find reliable methods for the estimation of construction material *** prices have a time dependent *** order to increase the foreseeability of the costs of construction materials,this study focuses on estimation of construction material indices through time series *** different types of analysis are implemented for estimation of the future values of construction material *** first method implemented was Autoregressive Integrated Moving Average(ARIMA),which is known to be successful in estimation of time series having a linear *** second method implementedwas Non-LinearAutoregressive Neural Network(NARNET)which is known to be successful in modeling and estimating of series with non-linear *** results have shown that depending on the nature of the series,both these methods can successfully and accurately estimate the future values of the *** addition,we found out that Optimal NARNET architectures which provide better accuracy in estimation of the series can be identified/discovered as result of grid search on NARNET hyperparameters.

关键词： Construction material indices ARIMA non-linear autoregressive neural network NARNETs

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