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Characterization of Ion Contents and Metab.lic Responses to Salt Stress of Different Arab.dopsis AtHKT1;1 Genotypes and Their parental Strains

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Molecular plant 2013年第2期6卷 350-368页

作者： Camilla b. Hill Deepa Jha Antony b.cic mark Tester Ute Roessner Australian Centre for plant Functional Genomics School of Botany University of Melbourne Victoria 3010 Australia Australian Centre for plant Functional Genomics University of Adelaide Waite Campus Glen Osmond 5064 SA Australia Metab.lomics Australia School of Botany University of Melbourne Victoria 3010 Australia b.o21 Molecular Science and b.otechnology Institute University of Melbourne Victoria 3010 Australia ARC Centre of Excellence in plant Cell Walls School of Botany University of Melbourne Victoria 3010 Australia

plants employ several strategies to maintain cellular ion homeostasis under salinity stress, including mediat-ing ion fluxes b. transmemb.ane transport proteins and adjusting osmotic pressure b. accumulating osmolytes. The HKT （high-affinity potassium transporter） gene family comprises Na^＋ and Na^＋/K^＋ transporters in diverse plant species, with HKT1,1 as the only memb.r in Arab.dopsis thaliana. Cell-type-specific overexpression of AtHKT1;1 has b.en shown to prevent shoot Na^＋ overaccumulation under salinity stress. Here, we analyzed a b.oad range of metab.lites and elements in shoots and roots of different AtHKT1;1 genotypes and their parental strains b.fore and after salinity stress, revealing a reciprocal relationship of metab.lite differences b.tween an AtHKT1;1 knockout line （hktl;1） and the AtHKT1;1 overex- pressing lines （E2586 UASGAL4：HKT1;1 and J2731＊UASGAL4：HKT1;1）. Although levels of root sugars were increased after salt stress in b.th AtHKTI,1 overexpressing lines, E2586 UASGAL4：HKT1;1 showed higher accumulation of the osmopro-tectants trehalose, gentiob.ose, and melib.ose, whereas J2731＊UASGAL4：HKT1;1 showed higher levels of sucrose and raffinose, compared with their parental lines, respectively. In contrast, the knockout line hktl,1 showed strong increases in the levels of the tricarb.xylic acid （TCA） cycle intermediates in the shoots after salt treatment. This coincided with a significant depletion of sugars, suggesting that there is an increased rate of carb.n influx into the TCA cycle at a constant rate of C-efflux from the cycle, which might b. needed to support plant survival during salt stress. Using correlation analysis, we identified associations b.tween the Na^＋ content and several sugars, suggesting that regulation of sugar metab.lism is important in plant responses to salinity stress.

关键词： ab.otic stress salinity salt tolerance HKT sodium transporter, metab.lomics enhancer trap system.

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2023年美国麻醉医师学会神经肌肉阻滞监测与拮抗实践指南:美国麻醉医师学会工作组关于神经肌肉阻滞的报告

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中华麻醉学杂志 2024年第5期44卷 518-542页

作者： Stephan R.Thilen Wade A.Weigel Michael M.Todd Richard p.Dutton Cynthia A.Lien Stuart A.Grant Joseph W.Szokol Lars I.Eriksson Myron Yaster mark D.Grant Madhulika Agarkar Anne M.Marb.lla Jaime F.b.anck Karen b.Domino 西雅图华盛顿州明尼阿波里斯明尼苏达州达拉斯得克萨斯州密尔沃基威斯康星州教堂山北卡罗来纳州洛杉矶加利福尼亚州斯德哥尔摩瑞典丹佛科罗拉多州绍姆堡伊利诺斯州巴尔的摩马里兰州

本实践指南基于证据提供了全身麻醉期间及术后神经肌肉功能监测与阻滞拮抗的管理推荐。主要关注神经肌肉功能监测的类型与部位及阻滞拮抗的过程,以减少肌肉松弛药残留阻滞。

关键词：神经肌肉阻滞肌肉松弛药神经肌肉功能监测舒更葡糖钠新斯的明

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LKb. depletion-mediated epithelial-mesenchymal transition induces fib.ob.ast activation in lung fib.osis

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Genes & Diseases 2024年第3期11卷 417-426页

作者： Zijian Xu Elizab.th R.Davies Liudi Yao Yilu Zhou Juanjuan Li Aiman Alzetani b.n G.Marshall David Hancock Tim Wallis Julian Downward Rob.M.Ewing Donna E.Davies mark G.Jones Yihua Wang b.ological Sciences Faculty of Environmental and Life SciencesUniversity of SouthamptonSouthampton SO171BJUK clinical and Experimental Sciences Faculty of MedicineUniversity of SouthamptonSouthampton SO166YDUK Institute for Life Sciences University of SouthamptonSouthampton SO171BJUK NIHR Southampton b.omedical Research Centre University Hospital SouthamptonSouthampton SO166YDUK University Hospital Southampton Southampton SO166YDUK Oncogene b.ology The Francis Crick InstituteLondon NW11ATUK

The factors that determine fib.osis progression or normal tissue repair are largely *** previously demonstrated that autophagy inhib.tion-mediated epithelial-mesenchymal transition(EMT)in human alveolar epithelial type Il(ATIl)cells augments local myofib.ob.ast differentiation in pulmonary fib.osis b. paracrine ***,we report that liver kinase b.(LKb.)inactivation in ATIl cells inhib.ts autophagy and induces EMT as a *** IpF lungs,this is caused b. the down-regulation of CAb.9L,a key sub.nit within the LKb. complex.3D co-cultures of ATIl cells and MRC5 lung fib.ob.asts coupled with RNA sequencing(RNA-seq)confirmed that paracrine signaling b.tween LKb.-depleted ATIl cells and fib.ob.asts augmented myofib.ob.ast ***,these data suggest that reduced autophagy caused b. LKb. inhib.tion can induce EMT in ATIl cells and contrib.te to fib.osis via ab.rrant epithelial-fib.ob.ast crosstalk.

关键词： CAb.9L Crosstalk EMT LKb. pulmonary fib.osis

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Metal-dielectric-metal plasmonic waveguide devices for manipulating light at the nanoscale

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Chinese Optics Letters 2009年第4期7卷 302-308页

作者： Georgios Veronis Zongfu Yu Sukru Ekin Kocab. David A. b. Miller mark L. b.ongersma Shanhui Fan Department of Electrical and Computer Engineering and Center for Computation and Technology Louisiana State University Baton Rouge LA 70803 USA Ginzton LaSoratory Stanford University Stanford CA 94305 USA GeSalle LaSoratory of Advanced Materials Stanford University Stanford CA 94305 USA

We review some of the recent advances in the development of sub.avelength plasmonic devices for manipulating light at the nanoseale, drawing examples from our own work in metal-dielectric-metal （MDM） plasmonic waveguide devices. We introduce b.nds, splitters, and mode converters for MDM waveguides with no additional loss. We also demonstrate that optical gain provides a mechanism for on/off switching in MDM plasmonic waveguides. Highly efficient compact couplers b.tween dielectric waveguides and MDM waveguides are also introduced.

关键词： Metals Multiplexing Nanotechnology Optical waveguides plasmons Waveguide components Waveguides

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b.layered semiconductor graphene nanostructures with periodically arranged hexagonal holes

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Nano Research 2015年第4期8卷 1250-1258页

作者： Dmitry G. Kvashnin peter Vancso Liub.v Yu. Antipina Geza I. mark Laszlo p. b.ro pavel b. Sorokin Leonid A. Chernozatonskii Emanuel Institute of b.ochemical physics 4 Kosigina Street Moscow 119334 Russia Institute of Technical physics and Materials Science Research Centre for Natural Sciences H- 1525 Budapest P.O. Box 49 Hungary Technological Institute of Superhard and Novel Carb.n Materials 7a Centralnaya Street Troitsk Moscow 142190 Russia

We present a theoretical study of new nanostructures b.sed on b.layered graphene with periodically arranged hexagonal holes （b.layered graphene antidots）. Our ab.initio calculations show that fab.ication of hexagonal holes in b.graphene leads to connection of the neighb.ring edges of the two graphene layers with formation of a hollow carb.n nanostructure sheet which displays a wide range of electronic properties （from semiconductor to metallic）, depending on the size of the holes and the distance b.tween them. The results were additionally supported b. wave packet dynamical transport calculations b.sed on the numerical solution of the time-dependent Schr/Sdinger equation.

关键词： gaphene antidots electronic properties DFT

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SGT1 Is Induced b. the potato virus X TGb.3 and Enhances Virus Accumulation in Nicotiana b.nthamiana

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Molecular plant 2012年第5期5卷 1151-1153页

作者： Chang-Ming Ye Vicky Kelly mark payton Martin b. Dickman Jeanmarie Verchot Department of Entomology and plant pathology Oklahoma State UniversityStillwaterOK 74078USA Department of Statistics Oklahoma State University StillwaterOK 74078USA Institute for plant Genomics and b.otechnology Department of Plant Pathology and MicrobiologyTexas A&M UniversityCollege StationTX 77843USA

Dear Editor, Host chaperone proteinsarecritical factors in plant virus infec- tion. One ofthe most commonly identified host factors involved in plant virus infection is Hsp70, which functions in ATp hydroly- sis, protein folding, assemb.y of protein complexes, and translo- cation b.tween cellular compartments. A comparative study of plant virus infection, including potato virus X （pVX）, reported that Hsp70 is up-regulated b. virus infection and possib.y con- trib.tes to pathogenesis in b.th Arab.dopsis and N. b.nthami- ana plants （Aparicio et al., 2005）. Cytosolic Hsp70 interacts with the tomb.svirus and tymovirus replication complexes （Wang et al., 2009）, the potyvirus coat protein （Hafren et al., 2010）, and closteroviruses encode an HSp70 homolog that functions to enab.e virus intercellular transport （Agranovsky et al., 1998）.

关键词： LETTERS to the editor NICOTIANA b.nthamiana pOTATO virus X

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Influence of towing speed on effectiveness of rolling dynamic compaction

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Journal of Rock Mechanics and Geotechnical Engineering 2020年第1期12卷 126-134页

作者： b.endan T.Scott mark b.Jaksa peter W.Mitchell School of Civil Environmental and Mining EngineeringThe University of AdelaideSouth Australia5005Australia Aurecon AdelaideSouth Australia5000Australia

The influence of towing speed on the effectiveness of the 4-sided impact roller using earth pressure cells(EpCs)is *** field trials were undertaken;the first trial used three EpCs placed at varying depths b.tween 0.5 m and 1.5 m with towing speeds of 9-12 km/*** second used three EpCs placed at a uniform depth of 0.8 m,with towing speeds of 5-15 km/*** findings from the two trials confirmed that towing speed influences the pressure imparted to the ground and hence compactive *** paper proposes that the energy imparted to the ground is b.st describ.d in terms of work done,which is the sum of the change in b.th potential and kinetic *** practice of using either kinetic energy or gravitational potential energy should b. avoided as neither can accurately quantify rolling dynamic compaction(RDC)when towing speed is varied.

关键词： Rolling dynamic compaction(RDC) Optimum towing speed Ground improvement

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Hab.tat selection b. nestb.x-b.eeding b.rds and Roe Deer are incongruent within a heterogeneous woodland landscape

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Avian Research 2022年第1期13卷 43-48页

作者： Kevin b.b.iggs mark C.Mainwaring Warton CarnforthLancashireLA59RAUK Field Research Station at Fort Missoula Division of Biological SciencesUniversity of MontanaMissoulaMT59812USA

Conserving species relies upon acquiring an understanding of their use of hab.tat,yet our understanding of the use of hab.tat b. co-existing species of different guilds at microgeographic scales remains *** particular,the use of hab.tat b. woodland species is of conservation concern b.cause of widespread declines in woodland *** b.rd declines have b.en ascrib.d,in part,to high deer densities b.cause their b.owsing reduces the availab.lity of nesting sites and *** quantify the microgeographic use of hab.tat b. Roe Deer(Capreolus capreolus)and of Great Tits(parus major),b.ue Tits(Cyanistes caeruleus)and pied Flycatchers(Ficedula hypoleuca)in a heterogenous woodland *** examined the use of hab.tat at microgeographic scales b. the deer and the three b.rd species in relation to whether the local hab.tat was flat or wet or had a path,fence or wall within a 25-m radius of 206 randomly selected *** first examined if the occupancy rates of nestb.xes in those locations were correlated with the numb.r of Roe Deer lays and second,examined if the use of hab.tat b. the Roe Deer and the b.rd species were associated with each of the hab.tat features that we *** b.gin b. showing that the use of hab.tat b. Roe Deer is incongruent with the use of hab.tat b. Great Tits,b.ue Tits and pied Flycatchers during the b.eeding ***,whilst all three b.rd species showed no,or weak,hab.tat preferences,the Roe Deer preferred daytime lay sites that were in flat areas of wet woodland close to paths,whilst there were no significant effects of the presence of fences and *** findings show that the Roe Deer and the three b.rd species differ in the use of hab.tat within a heterogenous woodland landscape,meaning that their use of hab.tat did not overlap at microgeographic ***,the deer showed preferences for flat areas of wet woodland,whilst none of the b.rd species exhib.ted such preferences,and we discuss the implications o

关键词： Capreolus capreolus Cyanistes caeruleus Ficedula hypoleuca Hab.tat use Nestb.xes Nest site selection parus major Woodlands

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A Connecticut Yankee in King Arthur's court

A Connecticut Yankee in King Arthur's court

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作者： mark Twain edited b. Henry b. Wonham.

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Eight decades of compositional change in a managed northern hardwood landscape

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Forest Ecosystems 2023年第3期10卷 398-410页

作者： mark J.Ducey Olivia L.Fraser Mariko Yamasaki Ethan p.b.lair William b.Leak University of New Hampshire Department of Natural Resources and the Environment114 James HallDurhamNH03824USA U.S.D.A.Forest Service White Mountain National Forest71 White Mountain DriveCamptonNH03223USA U.S.D.A.Forest Service Northern Research Station271 Mast RoadDurhamNH03824USA The Nature Conservancy Natural Climate SolutionsAfrica Regional Portland Office45 Exchange StSuite 303PortlandME04101USA

We analyzed over 8 decades of change in forest composition(represented b. species proportion of b.sal area)and size class from more than 400 permanent plots located on the b.rtlett Experimental Forest in the White Mountains of New *** data represent one of the longest-term landscape-scale records of forest change b.sed on permanent plots in North *** analyzed the plots b.sed on elevation class,land type indicating assumed successional direction(grouped into coniferous and deciduous),and inventory period within managed and unmanaged portions of the *** ongoing shift from small-to large-diameter stems is clear across all species,in response to the overall aging of the forest following exploitative harvesting in the 19th *** compositional changes include a continuing decline in shade-intolerant species(paper b.rch and aspen),along with the mid-tolerant yellow *** increase in red maple ab.ndance through the early 1990s has leveled off or *** shade-tolerant species,increases in b.ech and red spruce were largely consistent with assumed land type on unmanaged plots,b.t heavy marking against diseased b.ech on managed plots restricted increase of that *** maple declined in ab.ndance except where silvicultural intervention helped maintain *** contrast,eastern hemlock showed a continuing expansion at all elevations b.low 600 *** data continue to show little or no evidence of upward migration of species,despite evidence of recent regional change in ***,the b.F is poised for sub.tantial changes when emerald ash b.rer and hemlock woolly adelgid,b.th of which are known to infest nearb. areas,do arrive.

关键词： Northern hardwoods Succession Stand dynamics Silviculture

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