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Plant Communications 2020年第5期1卷 129-140页

作者： Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl Alisdair R.Fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Department of Biology Hong Kong Baptist Universityand State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongShatinHong Kong Institute for Developmental Genetics Heinrich Heine UniversityDusseldorfGermany

Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains ***-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis *** an efficient high-throughput transient expression system is currently lacking in the model plant species ***,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in *** and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and *** of this method was independently verified in three independent research facilities,pointing to the robustness of this ***,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

关键词： transient expression agro-infiltration subcellular localization protein-protein interaction

Mesenchymal stem cells secretome:The cornerstone of cell-free regenerative medicine

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World Journal of Stem Cells 2020年第12期12卷 1529-1552页

作者： Alberto González-González Daniel García-Sánchez Monica Dotta JoséC Rodríguez-rey Flor M Pérez-Campo Department of Molecular Biology_IDIVAL Faculty of MedicineUniversity of CantabriaSantander 39011CantabriaSpain

Mesenchymal stem cells(MSCs)are the most frequently used stem cells in clinical trials due to their easy isolation from various adult tissues,their ability of homing to injury sites and their potential to differentiate into multiple cell ***,the realization that the beneficial effect of MSCs relies mainly on their paracrine action,rather than on their engraftment in the recipient tissue and subsequent differentiation,has opened the way to cell-free therapeutic strategies in regenerative *** the soluble factors and vesicles secreted by MSCs are commonly known as *** secretome has a key role in cell-to-cell communication and has been proven to be an active mediator of immunemodulation and regeneration both in vitro and in ***,the use of secretome has key advantages over cell-based therapies,such as a lower immunogenicity and easy production,handling and ***,MSCs can be modulated to alter their secretome composition to better suit specific therapeutic goals,thus,opening a large number of *** these advantages now place MSCs secretome at the center of an important number of investigations in different clinical contexts,enabling rapid scientific progress in this field.

关键词： Mesenchymal stem cells Secretome Soluble factors Extra-cellular vesicles Exosomes Bone regeneration

Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars

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Protein & Cell 2012年第2期3卷 123-131页

作者： Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping Ko Chun-Hsiang Huang Feifei Ren Chun-Chi Chen Yanhe Ma rey-Ting Guo Yuanxia Sun Industrial Enzymes National Engineering Laboratory Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China Institute of Biological Chemistry Academia SinicaTaipei 115TaiwanChina CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in *** cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used *** this study,the crystal structure of the *** DPEase was *** enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active *** crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also *** the complex structures of *** DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site ***,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed *** results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis.

关键词： D-psicose 3-epimerase ketohexose complex structure

Single operator choledochoscopy and its role in daily endoscopy routine

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World Journal of Gastrointestinal Endoscopy 2013年第5期5卷 203-210页

作者： Arthur Hoffman Johannes Wilhelm rey Ralf Kiesslich Department of Interal Medicine St.Mary HospitalFrankfurt am Main60318 HessenGermany

Different diagnostic procedures exist for the detection of bile duct lesions in clinical ***,neither retrograde contrast imaging of the bile duct endoscopic retrograde cholangiopancreatogram nor other imaging procedures allow a safe diagnosis of the *** choledochoscopy may be a useful diagnostic procedure in macroscopic assessing lesions of the bile *** if the diagnostic sensitivity and specificity is not sufficient,first studies suggest an enhanced diagnostic accuracy for *** the progress of choledochoscopy has started in the 1970 different improvements were ***,the examination can be performed by an examiner and samples can be *** and Resolution quality has improved over the past years,*** SpyGlass system is a technically advanced cholangioscopic device to provide endoscopic diagnosis in case of inconclusive bile duct *** more,two more lumina allow specific biopsy forceps and optical fibers for electrohydraulic or laser *** most frequent useful insert of SpyGlass in clinical practice are in complex gallstones and bile duct lesions of unclear dignity.

关键词： Endoscopic retrograde cholangiography Endoscopic choledochoscopy SpyGlass Direct Visualization System TM 'Mother-baby' endoscope technique Gallstones Bile duct lesions

Food Habits and Distribution of the Lake Taal Sea Snake(Hydrophis semperi Garman 1881) and the Sympatric Little File Snake(Acrochordus granulatus Schneider 1799) in Lake Taal, Philippines

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Asian Herpetological Research 2014年第4期5卷 255-262页

作者： Vhon Oliver S.GARCIA rey Donne S.PAPA Jonathan Carlo A.BRIONES Norman MENDOZA Noboru OKUDA Arvin C.DIESMOS The Graduate School University of Santo Tomas Research Center for the Natural and Applied Sciences University of Santo Tomas Department of Biological Sciences University of Santo Tomas Department of Science and Technology Philippine Nuclear Research Institute Center for Ecological Research Kyoto University Herpetology Section Zoology Division National Museum of the Philippines

Our knowledge about the food habits of sea snakes and how it is associated with their distribution has seen much development through its description across a number of species available through published literature except for the key threatened species such asHydrophis semperi. This paper aims to describe the food habits ofH. semperi through gut content and stable isotope analyses. We also compared data with the Little File Snake,Acrochordus granulatus, sympatric withH. *** captures ofH. semperi suggest that the sea snake tends to occur in the littoral zones and the shallower portions of the limnetic zone. Gut content analysis ofH. semperi have shown that gobies and eels are primary prey items. Halfbeaks （Family Hemiramphidae） were recorded as one of the Lake Taal Sea Snake＇s prey items which is considered as a new prey record for sea snakes. These extracted gut contents are conifrmed to be the temporal food preference ofH. semperi given our detected stable isotope signatures. It appears thatA. granulatus and H. semperi share common prey items suggesting possible diet overlap and resource *** study reports the ifrst account of the endemic Lake Taal Sea Snake＇s distribution and food habits which poses implications towards its conservation as it occurs in a restricted ecosystem that has undergone considerable habitat alteration.

关键词： freshwater diet feeding Hydrophis semperi Hydrophiidae

Tuberculosis and hematopoietic stem cell transplant: Review of a difficult and often underestimated problem

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World Journal of Clinical Infectious Diseases 2013年第4期3卷 70-78页

作者： Guadalupe García-Elorriaga Guillermo del rey-Pineda Hospital for Infectious Disease "La Raza" National Medical Center Department of Infectious Disease and Intestinal Bacteriology Laboratory "Federico Gómez"Children’s HospitalDepartment of Health Central Blood Bank "La Raza"National Medical Center Social Security Mexican Institute

Recipients of solid organ transplants(SOT) and stem cell transplants(SCT) constitute a group of patients at risk for tuberculosis(TB) development. The prevalence of active TB in patients undergoing SOT is higher than in patients undergoing SCT, probably due to the shorter period of immunosuppression in the latter. We reviewed the importance of SCT in individuals with hematological malignancies. Most TB cases occur in transplant patients by reactivation of latent infection after immunosuppression, most often within the first year after transplant, leading to graft loss and in some cases, death. Relevant variables to assess the risk of TB infection in a transplant recipient include the donor's and recipient's medical histories, imaging results, microbiology and tuberculin skin test(TST) and interferon-gamma release assays(IGRA). TST is routinely performed in the donor and recipient before transplantation. If TST is > 5 mm in the recipient or > 10 mm in the donor, it is necessary to exclude active TB(pulmonary and renal). Chemopro-phylaxis is recommended in TST(+) recipients and in recipients with recent seroconversion, in donors with a history of untreated TB or in contact with an individual with active TB, if radiological images are suspicious and the IGRA is(+). The drug of choice is isoniazid. These topics are herewith reviewed.

关键词： Tuberculosis Prophylaxis Transplant Solid organ transplantation Hematopoietic stem cell transplantation

Uncertainty Propagation of Structural Computation for Fatigue Assessment

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Journal of Marine Science and Application 2022年第4期21卷 55-66页

作者： Ludovic Mell Valentine rey Franck Schoefs Benjamin Rocher Nantes Université École Centrale NantesCNRSGeMUMR 6183F-44000 NantesFrance Chantiers de l’Atlantique Avenue Bourdelle-CS90180St-Nazaire CEDEX 44613France

Offshore wind substations are subjected to uncertain loads from waves,wind and *** states are composed of irregular waves which statistics are usually *** loads may induce fatigue failure of some structural components of the *** combining fatigue damage computed through numerical simulations for each sea state endured by the structure,it is possible to assess fatigue failure of the structure over the whole deployment ***,the influence of the discretization error on the fatigue damage is rarely *** is possible to estimate the discretization error on the quantity of interest computed at the structural detail suspected to ***,the relation between this local quantity of interest and the fatigue damage is *** this paper,a method that allows propagating error bounds towards fatigue damage is *** increasing computational burden,computing discretization error bounds is a useful output of finite element *** can be utilized to either validate mesh choice or guide remeshing in case where potential error on the fatigue damage is too *** method is applied to an offshore wind substation developped by Chantiers de l’Atlantique using two discretization error estimators in a single sea state.

关键词： Offshore wind turbines Uncertainty propagation Fatigue damage Discretization error

New aspects of modern endoscopy

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World Journal of Gastrointestinal Endoscopy 2014年第8期6卷 334-344页

作者： Johannes Wilhelm rey Ralf Kiesslich Arthur Hoffman Department of Internal Medicine St. Mary’s Hospital

The prognosis for patients with malignancies of the ga-strointestinal-tract is strictly dependent on early detec-tion of premalignant and malignant lesions. However, small, flat or depressed neoplastic lesions remain dif-ficult to detect with these technologies thereby limiting their value for polyp and cancer screening. At the same time computer and chip technologies have undergone major technological changes which have greatly im-proved endoscopic diagnostic investigation. New imag-ing modalities and techniques are very notable aspects of modern endoscopy. Chromoendoscopy or filter-aided colonoscopy(virtual chromoendoscopy) with high defi-nition endoscopes is able to enhance the detection and characterization of lesions. Finally, confocal laser en-domicroscopy provides histological confirmation of the presence of neoplastic changes. The developing tech-niques around colonoscopy such as the retro-viewing colonoscope, the balloon-colonoscope or the 330-de-grees-viewing colonoscope try to enhance the efficacy by reducing the adenoma miss rate in right-sided, non-polypoid lesions. Colon capsule endoscopy is limited to identifying cancer and not necessarily small adenomas. Preliminary attempts have been made to introduce this technique in clinical routine.

关键词： Modern endoscopy High definition endos-copy Virtual chromoendoscopy Autofluorescence En-domicroscopy Molecular imaging

Experimental test of a building thermal system for preventive maintenance based on thermoeconomic analysis

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Energy Storage and Saving 2024年第1期3卷 42-51页

作者： I.Prol-Godoy A.Picallo-Perez J.M.Sala-Lizarraga J.rey-Martínez Research group ENEDI Department of Thermal EngineeringUniversity of the Basque Country(UPV/EHU)Bilbao48013Spain Research Group Termotecnia University of ValladolidValladolid47002Spain

An analysis of the sectorial structure of energy consumption shows that residential and tertiary sector buildings are the third-highest consumers,responsible for 29.5%of a city’s final energy *** Building Quality Control Laboratory of the Basque Government aims to promote quality,innovation,and sustainability in *** accomplish this goal,it has constructed an experimental facility with different energy generation technologies and a very versatile control system for testing different energy systems and operation *** this study,we tested a facility for supplying domestic hot water and heating for a multi-family house by means of a condensing boiler and an aerothermal heat pump(together with the corresponding control).This installation could reproduce the thermal demands required to be satisfied by the generation equipment through a programmed operation of the installation based on real ***,this installation was analyzed using thermoeconomics(TE)to solve problems unable to be solved using traditional energy analyses based on the First Law of *** problems include:(1)Determining the costs of the products of the installation based on physical criteria,(2)detecting the places where losses actually occur,evaluating their costs,and proposing cost-effective improvements,and(3)diagnosing issues in the *** a result,this paper suggests a solution to the preventive maintenance problems confronting the technical maintenance personnel for thermal installations in buildings by applying TE knowledge and using real data collected from sensors.

关键词： Experimental essay Energy savings in thermal systems Thermoeconomics Thermoeconomic costs Diagnosis

Perceptions of the Role Played by Aquaculture and the Services It Provides for Territories: Complementarity of Survey Types

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Open Journal of Ecology 2018年第4期8卷 270-285页

作者： Syndhia Mathé Hélène rey-Valette UMR Innovation TA C-85/15 73 rue Jean-Fran&#231 ois Breton 34398 Montpellier Cedex 5 Montpellier France INNOVATION Univ Montpellier CIRAD INRA Montpellier SupAgro Montpellier France IITA Cameroun Yaoundé Cameroun Centre d’Economie de l’Environnement-Montpellier (CEE-M) and LAMETA Univ. Montpellier CNRS INRA SupAgro France

This article discusses the perception of ecosystem services for French and Brazilian pond fish farming. Its aim is to study the extent to which the industry and its stakeholders are aware of the existence of such services in order to be able to prioritize them with respect to social well-being. This identification phase is seldom discussed. It requires that survey questionnaires be designed so as to improve understanding of these perceptions. The surveys undertaken combined both open questions where the interviewees expressed their perceptions spontaneously and closed questions where they were invited to rank a pre-established list of the relevant services. These surveys involved 133 fish farmers and 93 stakeholders. The results show that perceptions differ by question type, and confirm the significance of the questionnaire design and the importance of combining several kinds of approach.

关键词： Ecosystem Services Ponds Fish Farming Questionnaire Design France Brazil