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New solutions for combatting implant bacterial infection based on silver nano-dispersed and gallium incorporated phosphate bioactive glass sputtered films:A preliminary study

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Bioactive Materials 2022年第2期7卷 325-340页

作者： B.W.Stuart G.E.stan A.C.Popa M.J.Carrington I.Zgura M.Necsulescu D.M.Grant m.grantadvanced materials research group faculty of engineeringuniversity of nottinghamnottinghamng72rduk national institute of materials physics magurelero077125romania army centre for medical research bucharestro010195romania

Ag/Ga were incorporated into resorbable orthopaedic phosphate bioactive glasses(PBG,containing P,Ca,Mg,Na,and Fe)thin films to demonstrate their potential to limit growth of Staphylococcus aureus and Escherichia coli in post-operative prosthetic *** target consecutive co-sputtering was uniquely employed to produce a 46 nm Ag:PBG composite observed by high resolution TEM to consist of uniformly dispersed~5 nm metallic Ag nano-particles in a glass ***^(3+)was integrated into a phosphate glass preform target which was magnetron sputtered to film thicknesses of~400 or 1400 *** coatings exhibited high surface energy of 75.4-77.3 mN/m,attributed to the presence of hydrolytic P-O-P structural surface *** profiles obtained in deionized water,nutrient broth and cell culture medium showed varying ion release profiles,whereby Ga release was measured in 1400 nm coating by ICP-MS to be~6,27,and 4 ppm respectively,fully dissolving by 24 *** of Ag nanoparticles was only observed in nutrient broth(~9 ppm by 24 h).Quantification of colony forming units after 24 h showed encouraging antibacterial efficacy towards both ***(4-log reduction for Ag:PBG and 6-log reduction for Ga-PBG≈1400 nm)and ***(5-log reduction for all physical vapour deposited layers)*** Hs27 fibroblast and mesenchymal stem cell line in vitro tests indicated good cytocompatibility for all sputtered layers,with a marginal cell proliferation inertia in the case of the Ag:PBG composite thin *** study therefore highlights the(i)significant manufacturing development via the controlled inclusion of metallic nanoparticles into a PBG glass matrix by dual consecutive target co-sputtering and(ii)potential of PBG resorbable thin-film structures to incorporate and release cytocompatible/antibacterial *** architectures showed prospective bio-functional performance for a future generation of endo-osseous implant-type coatings.

关键词： Phosphate bioactive glass Silver Gallium Antibacterial Implant coating Magnetron sputtering

Dynamics of Psychopathology Symptoms in Opiate-Dependent Patients During Therapy

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Psychology Research 2015年第12期5卷 693-700页

作者： Igumnov SergeyAleksandrovich stan'ko Edward Pavlovich Nestsiarovich Anastasiya Nikolayevna Serbsky Federal Medical ResearchCentre of Psychiatry and Narcology Ministry of Health of the Russian Federation Moscow Russia Educational Institution GrodnoState Medical University Grodno Belarus Belarusian State Medical University Minsk Belarus

Logical Function Decomposition Method for Synthesis of Digital Logical System Implemented with Programmable Logic Devices (PLD)

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Circuits and Systems 2013年第7期4卷 472-477页

作者： Mihai Grigore Timis Alexandru Valachi Alexandru Barleanu Andrei stan Automatic Control and Computer Engineering Faculty Technical University Gh.Asachi Iasi Romania

The paper consists in the use of some logical functions decomposition algorithms with application in the implementation of classical circuits like SSI, MSI and PLD. The decomposition methods use the Boolean matrix calculation. It is calculated the implementation costs emphasizing the most economical solutions. One important aspect of serial decomposition is the task of selecting “best candidate” variables for the G function. Decomposition is essentially a process of substituting two or more input variables with a lesser number of new variables. This substitutes results in the reduction of the number of rows in the truth table. Hence, we look for variables which are most likely to reduce the number of rows in the truth table as a result of decomposition. Let us consider an input variable purposely avoiding all inter-relationships among the input variables. The only available parameter to evaluate its activity is the number of “l”s or “O”s that it has in the truth table. If the variable has only “1” s or “0” s, it is the “best candidate” for decomposition, as it is practically redundant.

关键词： Combinational Circuits Static Hazard Logic Design Boolean Functions Logical Decompositions

Security Improvement of the Protection of Mobile Networks 3G Communication Channels, Based on Chaotic Cryptographic Algorithms

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通讯和计算机（中英文版） 2013年第3期10卷 346-354页

作者： Ion Sima Daniel Tarmurean Adrian-Viorel diaconu Valentin stan IT&C Department Lumina- The University of South-East Europe Bucharest 021187 Romania ETTI Faculty Politehnica University of Bucharest Bucharest 060042 Romania TET Faculty Politehnica University of Bucharest Bucharest 060042 Romania

Two Analytical Methods for Detection and Elimination of the Static Hazard in Combinational Logic Circuits

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Circuits and Systems 2013年第7期4卷 466-471页

作者： Mihai Grigore Timis Alexandru Valachi Alexandru Barleanu Andrei stan Automatic Control and Computer Engineering Faculty Technical University Gh.Asachi Iasi Romania

In this paper, the authors continue the researches described in [1], that consists in a comparative study of two methods to eliminate the static hazard from logical functions, by using the form of Product of Sums (POS), static hazard “0”. In the first method, it used the consensus theorem to determine the cover term that is equal with the product of the two residual implicants, and in the second method it resolved a Boolean equation system. The authors observed that in the second method the digital hazard can be earlier detected. If the Boolean equation system is incompatible (doesn’t have solutions), the considered logical function doesn’t have the static 1 hazard regarding the coupled variable. Using the logical computations, this method permits to determine the needed transitions to eliminate the digital hazard.

关键词： Combinational Circuits Static Hazard Logic Design Boolean Functions

Intelligent designs in nanophotonics:from optimization towards inverse creation

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PhotoniX 2021年第1期2卷 4-38页

作者： Ning Wang Wei Yan Yurui Qu Siqi Ma stan Z.Li Min Qiu Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province School of EngineeringWestlake University18 Shilongshan RoadHangzhouZhejiang ProvinceChina Institute of Advanced Technology Westlake Institute for Advanced Study18 Shilongshan RoadHangzhouZhejiang ProvinceChina Department of Electrical and Computer Engineering University of Wisconsin-Madison1415 Engineering Dr.Madison 53706WI USA AI lab School of EngineeringWestlake University18 Shilongshan RoadHangzhouZhejiang ProvinceChina.

Applying intelligence algorithms to conceive nanoscale meta-devices becomes a flourishing and extremely active scientific topic over the past few *** design of functional nanostructures is at the heart of this topic,in which artificial intelligence(AI)furnishes various optimization toolboxes to speed up prototyping of photonic layouts with enhanced *** this review,we offer a systemic view on recent advancements in nanophotonic components designed by intelligence algorithms,manifesting a development trend from performance optimizations towards inverse creations of novel *** illustrate interplays between two fields,AI and photonics,we take meta-atom spectral manipulation as a case study to introduce algorithm operational principles,and subsequently review their manifold usages among a set of popular *** arranged from levels of individual optimized piece to practical system,we discuss algorithm-assisted nanophotonic designs to examine their mutual *** further comment on a set of open questions including reasonable applications of advanced algorithms,expensive data issue,and algorithm benchmarking,***,we envision mounting photonic-targeted methodologies to substantially push forward functional artificial meta-devices to profit both fields.

关键词： Artificial intelligence Nanophotonics Metasurface Optical neural network Intelligent designs

Comparison of Five Expression Vectors for the Ha Gene in Constructing a DNA Vaccine for H6N2 Influenza Virus in Chickens

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Advances in Microbiology 2016年第4期6卷 310-319页

作者： Songhua Shan Trevor Ellis John Edwards stan Fenwick Ian Robertson School of Veterinary and Life Sciences Murdoch University Perth Australia

A number of eukaryotic expression vectors have been developed for use as DNA vaccines. They showed varying abilities to initiate immune responses;however, there is little data to indicate which of these vectors will be the most useful and practical for DNA vaccines in different species. This report examines the use of five expression vectors with different promoters and Kozak sequence to express the same hemagglutinin (HA) protein of an H6N2 avian influenza virus for DNA vaccination in chickens. Although intramuscular vaccination with seven DNA constructs elicited no or limited measurable H6 HA antibody responses in Hy-Line chickens, variable reduction in virus shedding for either oropharyngeal or cloacal swabs post-virus challenge were observed. This indicated that all DNA constructs generated some levels of protective immunity against homologous virus challenge. Interestingly, lower dose (50 or 100 μg) of plasmid DNAs consistently induced better immune response than higher dose (300 or 500 μg). In the transfection experiments there appeared to be a hierarchy in the in vitro expression efficiency in the order of pCAG-optiHAk/ pCAG-HAk > pCI-HAk > VR-HA > pCI-HA > pCI-neo-HA > pVAX-HA. Since the level of in vitro expression correlates with the level of immune response in vivo, in vitro expression levels of the DNA constructs can be used as an indicator for pre-selection of plasmid vaccines prior to in vivo assessment. Moreover, our results suggested that the Kozak sequence could be used as an effective tool for DNA vaccine design.

关键词： DNA Vaccine Multiple Expressing Vectors H6N2 Avian Influenza a Virus Chickens

A single-stage bilayered skin reconstruction using Glyaderm■as an acellular dermal regeneration template results in improved scar quality:an intra-individual randomized controlled trial

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Burns & Trauma 2023年第1期11卷 408-428页

作者： Ignace De Decker Henk Hoeksema Jozef Verbelen Petra De Coninck Marijn Speeckaert Sofie De Schepper Phillip Blondeel Ali Pirayesh stan Monstrey Karel E.Y.Claes Burn Center Ghent University HospitalC.Heymanslaan 109000 GhentBelgium Department of Plastic Surgery Ghent University HospitalC.Heymanslaan 109000 GhentBelgium Department of Nephrology Ghent University HospitalC.Heymanslaan 109000 GhentBelgium Department of Dermatology Ghent University HospitalC.Heymanslaan 109000 GhentBelgium Plastic surgeon in private practice in Amsterdam AmsterdamThe Netherlands

Background:Absence of almost the entire reticular dermal layer is inherent to the use of autologous split-thickness skin grafting(STSG)to close full-thickness wounds,often resulting in hypertrophic scars and *** dermal substitutes have been developed,but unfortunately most have varying results in terms of cosmetic and/or functional improvement as well as patient satisfac-tion,in addition to high *** skin reconstruction using the human-derived glycerolized acellular dermis(Glyaderm■)has been reported to result in significantly improved scar quality using a two-step *** the necessary two-step procedure for most commercially available dermal substitutes,in this study we aimed to investigate the use of Glyaderm■in a more cost-effective single-stage *** is a method which,if autografts are available,is preferred by the majority of surgeons given the reduction in costs,hospitalization time and infection ***:A prospective,randomized,controlled,intra-individual,single-blinded study was per-formed,investigating the simultaneous application of Glyaderm■and STSG *** alone in full-thickness burns or comparable deep skin *** the acute phase,bacterial load,graft take and time to wound closure were assessed and were the primary *** and functional results(secondary outcomes)were evaluated at 3,6,9 and 12 months follow-up using subjective and objective scar measurement *** for histological analysis were taken at 3 and 12 ***:A total of 66 patients representing 82 wound comparisons were *** take(>95%),pain management and healing time were comparable in both *** 1 year follow-up,the overall Patient and Observer Scar Assessment Scale assessed by the patient was significantly in favour of sites where Glyaderm■was *** infrequently,patients attributed this difference to improved skin *** analysis showed the presence of a well-formed neoder

关键词： Burn Scar Skin Glyaderm■ Dermal substitute Dermal regeneration template Elastin

Population Characteristics of Ozark Bass (<i>Ambloplites constellatus</i>) in the Upper White River Basin of Northern Arkansas

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Natural Resources 2019年第5期10卷 121-138页

作者： Ashley Rodman Kristofor R. Brye Daniel Magoulick stan Todd Buffalo National River National Park Service Harrison AR USA Department of Crop Soil and Environmental Sciences University of Arkansas Fayetteville USA U.S. Geological Survey Arkansas Cooperative Fish and Wildlife Research Unit Department of Biological Sciences University of Arkansas Fayetteville USA Arkansas Game and Fish Commission Little Rock AR USA

Ozark Bass (Ambloplites constellatus) is an understudied, endemic fish species in the Upper White River Basin of northern Arkansas. This study was part of an effort by fisheries managers to gather baseline data about the Ozark Bass to aid in understanding population dynamics and contribute to the limited data available for use in determining the efficacy of harvest regulations. Select population characteristics of Ozark Bass in two northern Arkansas streams were determined, population characteristics of Ozark Bass were compared to Shadow Bass (Ambloplites ariommus) and Rock Bass (Ambloplites rupestris) data collected from previous studies in southern Missouri, and relative condition, length-at-age, and annual survival of Ozark Bass were compared between sample streams. Sampling occurred in Crooked Creek and the Buffalo River during summer 2013 via boat electroshocking. Length and weight data were recorded for all Ozark Bass collected, and fish ages were determined through selective otolith retrieval and age-length keys. Ozark Bass in Crooked Creek had greater relative condition than Ozark Bass in Buffalo River (P P > 0.05) between sexes for fish collected from only the Buffalo River. Ozark Bass mean annual survival was similar between Crooked Creek (55% ± 5% as 95% confidence interval (CI)) and the Buffalo River (50% ± 7% CI) for fish age 2 to 9. Calculated Ozark Bass lengths-at-age for fish from both streams were comparable to the Von Bertalanffy growth estimates, except the Buffalo River age 7 category where there was only one observation. The relationship between Ozark Bass age and length differed between sampled streams, and variability in growth rates and length-at-age were observed among Ambloplites species. Results of this study contribute to the understanding of the population dynamics of the Ozark Bass that will lead to improved fisheries management.

关键词： Endemic Fish Measurements Fishing Regulations Ozarks