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Detection of aac(3)IIc, aac(6)Ib, armA Genes Coding for Escherichia coli Resistance to Aminoglycosides in Burkina Faso

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Advances in Infectious Diseases 2023年第4期13卷 574-585页

作者： Pamane Djagbare Christelle Nadembega Tani Sagna Abdoul Karim Ouattara Emmanuel Sampo Théodora Zohoncon Moussa Ouedraogo marius belemgnegre Dorcas Obiri-Yeboah Jacques Simpore Laboratory of Molecular Biology and Genetics LaBioGene Joseph KI-ZERBO University Ouagadougou Burkina Faso Institute for Research in Health Sciences/National Center for Scientific and Technological Research IRSS-CNRST Ouagadougou Burkina Faso Biomedical Laboratory of the Schiphra Hospital Ouagadougou Burkina Faso Biomedical Laboratory of Saint Camille Hospital HOSCO Ouagadougou Burkina Faso School of Medical Sciences Department of Microbiology and Immunology University of Cape Coast University Post Office Cape Coast Ghana

Background and Prupose: Antibiotic resistance is a major global health concern. In addition to the existing data on the prevalence of bacterial resistance to antibiotics, there are patchy data on bacterial resistance to aminoglycosides in Burkina Faso. In this study, we determined the prevalence of aminoglycoside resistance genes in E. coli, including aac(3)-IIc, aac(6)-Ib and armA in Ouagadougou, and determined which antibiotics in this class are most affected by resistance. Material and Methods: This study was conducted on 216 E. coli strains collected from the biomedical analysis laboratories of Saint Camille and Schiphra hospitals. E. coli strains were isolated from pus and urine samples collected between September 2018 and January 2019. Antibiotic susceptibility testing was performed using aminoglycosides, β-lactams, fluoroquinolones, and sulfonamides. Aminoglycoside resistance genes were detected in strains with at least one aminoglycoside resistance gene using conventional/multiplex PCR. Results: Aminoglycoside resistance was observed in 46.8% (101/216) of strains. The resistance rates were respectively 45.37% for Tobramycin, 32.40% for Gentamicin, 14.81% for Kanamycin, 2.31% for Netilmicin, 1.84% for Neomycin, and 0.46% for Amikacin. PCR showed that 86 strains (85.15%) possessed the aac(3)-IIc gene, 71 strains or 70.30%) possessed the aac(6’)-Ib gene, and nine strains (8.91%) possessed the armA gene. Conclusion: Aminoglycoside resistance in pathogenic E. coli strains is mainly due to the presence of the aac(3’)-IIc and aac(6’)-Ib genes. The presence of armA was first reported in Burkina Faso. Netilmicin, Neomycin and Amikacin are good therapeutic options for treating urinary tract and pus-forming infections.

关键词： E. coli Aminoglycoside Resistance acc(3’)-IIc aac(6’)-Ib armA Burkina Faso

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Hepatitis B and C Immunological and Molecular Parameter Analysis in HIV-Positive Patients Undergoing Antiretroviral Therapy at Saint Camille Hospital in Ouagadougou (HOSCO), Burkina Faso

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Advances in Infectious Diseases 2022年第1期12卷 20-41页

作者： Denise P. Ilboudo W. W. Eudoxie Kévine Savadogo Théodora M. Zohoncon Estelle Savadogo Yéri Esther Hien Lassina Traore Djéneba Ouermi Wendkuuni Florencia Djigma Christelle M. Nadembega marius belemgnegre Paul Ouedraogo Mahamoudou Sanou Damintoti Simplice Karou Yves Traore Jacques Simpore Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT University Joseph KI-ZERBO Ouagadougou Burkina Faso University of Fada N’Gourma Fada N’Gourma Burkina Faso Laboratory of Biochemistry and Applied Immunology University Joseph KI-ZERBO Ouagadougou Burkina Faso Biomolecular Research Center Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Université Saint Thomas d’Aquin Faculté de Médecine Ouagadougou Burkina Faso Hopital Saint Camille Ouagadougou (HOSCO) Ouagadougou Burkina Unité de Formation et de Recherche en Science De la Santé (UFR/SDS) Université Joseph KI-ZERBO (UJKZ) Ouagadougou Burkina Faso Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA-UL) Université de Lomé Lomé Togo

Knowledge of the clinical and biological profile of patients infected with HIV and hepatitis B and/or C is essential in order to identify and implement effective management strategies. Methods: This was a retrospective descriptive study from January 01, 2016 to June 01, 2021. Adult patients aged at least 18 years infected with HIV type 1 and/or 2, na?ve to ARV treatment. Univariate analyses were assessed using Pearson’s Chi2 test. The Student Newman test was used for comparison between groups using R software version 4.0.2. Objective: To draw up the epidemiological, clinical, paraclinical and evolutionary profiles of HIV-treated-patients in relation to HIV/HBV and HIV/HCV co-infections in order to allow the identification and the implementation of effective management strategies. Results: Of the 379 patients included 280 (73.88%) were women. At treatment initiation, the mean age was 40.14 ± 11.84 years. The majority of patients consulted at WHO stage III (51.45%). Clinical suspicion was the most frequent screening circumstance (51.71%). The pathologies frequently reported at the first consultation were diarrhea (28%) and shingles (16%). Body mass index was normal in 50.5% of patients. HIV1 infection was the majority (91.03%). A total of 270 had a CD4 count at treatment initiation. The mean CD4 cell count was 304.17 ± 242.06 cells/μL, and 116 (42.59%) of them had a CD4 ≤ 200 cells/μL. Viral load at treatment initiation was documented in 62 patients (16.35%) and 70.97% of them had a detectable viral load (greater than 1000 copies/mL). The clinical and biological evolution was relatively good in patients after therapeutic initiation. HIV-HBV co-infection was 24.11% and HIV-HCV co-infection was 2.26%. The mortality rate was 3.69%. Conclusion: These results reflect a significant delay in HIV infection diagnosis. Furthermore, hepatitis B and/or C is co-infections that increasingly affect people living with HIV. It also appears that COVID 19 disease has had a strong impact

关键词： HIV Clinical-Biological Profile Hepatitis B and C Co-Infection Burkina Faso

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