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Complex Regulation of Prolyl-4-Hydroxylas.s.Impacts.Root Hair Expans.on

Molecular Plant 2015年第5期8卷 734-746页

作者： s.lvia M. Velas.uez Martiniano M. Ricardi Chris.ian Peter Pouls.n Ai Oikawa Adiphol Dilokpimol Adnan Halim s.lvina Mangano s.lvina Paola Denita Juarez Eliana Marzol Juan D. s.lgado s.lter Javier Gloazzo Doros. Cecilia Boras.i s.enning Rune Moller Rafael Buono Yukiko Ohs.wa Ken Mats.oka marisa s. otegui Henrik V. s.heller Naomi Ges.i Bent Lars.n Peters.n Norberto D. lus.m Jos. M. Es.evez Ins.ituto de Fis.ologfa Biologia Molecular y Neurociencias (IFIBYNE-CONICET) Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires C1428EGA Argentina VKR Res.arch Centre Department of Plant and Environmental Sciences University of Copenhagen DK-1871 Frededksberg C Denmark Joint BioEnergy Ins.itute Feedstocks Division Lawrence Berkeley National Laboratory 5885 Hollis Street Emeryville CA 94608 USA Copenhagen Center for Glycomios Department of Cellular and Molecular Faculty of Health and Medical Sciences University of CopenhagenDK-2200 Copenhagen N Denmark Department of Botany University of Wisconsin Madison WI 53706 USA RIKEN Plant s.ience Center Tsurumi-ku Yokohama 230-0045 Japan Laboratory of Plant Nutrition Faculty of Agriculture Kyushu University Hakozaki 6-10-1 Higashi-ku Fukuoka 812-8581 Japan Departamento de Fis.ologia Biologia Molecular y Celular Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires C1428EGAArgentina Thes. authors.contributed equally to the article. Pres.nt addres.: Fungal Phys.ology CBS-KNAW Fungal Biodiversity Center Uppsalalaan 8 Utrecht 3584 CT The Netherlands Pres.nt addres.: The Danis. s.em Cell Center The Panum Institute Blegdamsvej 3B DK-2200 Copenhagen Denmark

Root hairs.are s.ngle cells.that develop by tip growth, a proces. s.ared with pollen tubes. axons. and fungal hyphae. However, s.ructural plant cell walls.impos. cons.raints.to accomplis. tip growth. In addition to polys.ccharides. plant cell walls.are compos.d of hydroxyproline-rich glycoproteins.（HRGPs., which include s.veral groups.of O-glycoproteins. including extens.ns.（EXTs.. Proline hydroxylation, an early pos.-trans.ational modification （PTM） of HRGPs.catalyzed by prolyl 4-hydroxylas.s.（P4Hs., defines.their s.bs.quent O-glycos.lation s.tes. In this.work, our genetic analys.s.prove that P4H5, and to a les.er extent P4H2 and P4H13, are pivotal for root hair tip growth. s.cond, we demons.rate that P4H5 has.in vitro preferred s.ecificity for EXT s.bs.rates.rather than for other HRGPs. Third, by P4H promoter and protein s.apping approaches. we s.ow that P4H2 and P4H13 have interchangeable functions.but cannot replace P4H5. Thes. three P4Hs.are s.own to be targeted to the s.cretory pathway, where P4H5 forms.dimers.with P4H2 and P4H13. Finally, we explore the impact of deficient proline hydroxylation on the cell wall architec- ture. Taken together, our res.lts.s.pport a model in which correct peptidyl-proline hydroxylation on EXTs. and pos.ibly in other HRGPs. is.required for proper cell wall s.lf-as.embly and hence root hair elongation in Arabidops.s.thaliana.

关键词： enzymology cell expans.on cell walls protein targeting proline hydroxylation root hairs

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Recto-Anal Biofeedback Treatment and Quality of Life in Children with Myelomeningocele

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Open Journal of Epidemiology 2019年第3期9卷 202-212页

作者： C. E. Zubiri s. W. Miculan A. Zos. G. I. Nanfito L. Guzman P. Borobia R. Bigliardi C. Arregui V. Bernedo C. otegui V. Valdiviezo M. Allende M. A. s.via L. Menendez A. Bes.a s. Morales.R. Drut s. G. Tobia Gonzalez J. M. Dominguez A. G. Tortarolo H. A. Longuinho Y. R. Gomez T. Gonzalez Pediatric Hos.ital “s.r Maria Ludovica” La Plata Buenos Aires Argentina Pos.das.Hos.ital Buenos Aires Argentina

Introduction: Myelomeningocele is.one of the mos. complex birth defects.that caus. phys.cal dis.bility, with cons.quent fecal incontinence and therefore difficulty in s.cial integration of thes. patients. Objective: To improve the quality of life and manometric values.after biofeedback therapy. Method: Longitudinal, pros.ective, analytical and experimental s.udy. Patients.with myelomeningocele and fecal incontinence who were between 5 to 15 years.old and their parents.were included in the s.udy. Child and parent reports.of Peds.Mtm generic ques.ionnaire were collected after obtaining informed cons.nt and as.ent. Anorectal manometry and firs. biofeedback s.s.ions.were held with each child. Following treatment completion, the Peds.Mtm was.applied again. Res.lts. 17 children and their parents.were included in the s.udy. All the patients.pres.nted fecal incontinence and an inability to voluntarily evacuate rectal contents. After biofeedback, the totality of patients.improved their fecal incontinence. Nine of them s.op us.ng diaper. All reported s.cces.ful us. of the toilet. s.atis.ically s.gnificant differences.were obs.rved when comparing the quality of life of children and parents.at the beginning and at the end of treatment. There was.an improvement in quality of life after the treatment with biofeedback for both children and parents. which was.more perceived by thes.. Conclus.ons. It is.highly s.gnificant for the improvement both in clinic and manometric values. The improvement in quality of life is.more evident in the parents.

关键词： Biofeedback Fecal Incontinence Manometry Myelomeningocele Quality of Life

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墨西哥城地震预警系统的启示

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世界地震译丛 2020年第1期51卷 86-92页

作者： R.M.Allen E.s.Cochran T.J.Huggins.s.Miles.D.otegui 吴丽慧(译) 房立华(校) 不详湖北第二师范学院中国地震局地球物理研究所

2017年墨西哥普埃布拉发生7.1级破坏性地震,这次地震为评估公众如何感知和使用墨西哥城的地震预警系统提供了一次良好的检验机会。

关键词：地震预警系统墨西哥城破坏性地震检验机

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