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Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYp2C9 enzyme in permeabilized Saccharomyces cerevisiae cells

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Synthetic and Systems Biotechnology 2024年第3期9卷 406-415页

作者： Rana Azeem Ashraf Matthias Bureik mario Andrea Marchisio School of pharmaceutical Science and Technology Tianjin University300072TianjinChina

Gene circuits allow cells to carry out complex functions such as the precise regulation of biological metabolic *** this study,we combined,in the yeast ***,genetic regulatory elements with the enzymatic reactions of the human CYp2C9 and its redox partner CpR on luciferin substrates and *** cells were permeabilized and used as enzyme bags in order to host these metabolic *** engineered three different(genetic)-enzymatic basic Boolean gates(YES,NOT,and N-IMpLY).In the YES and N-IMpLY gates,human CYp2C9 was expressed under the galactose-inducible GAL1 *** carbon monoxide releasing molecule CORM-401 was used as an input in the NOT and N-IMpLY gates to impair CYp2C9 activity through inhibition of the Fe+2-heme prosthetic group in the active site of the human *** study provides a new approach in designing synthetic bio-circuits and optimizing experimental conditions to favor the heterologous expression of human drug metabolic enzymes over their endogenous *** new approach will help study precise metabolic attributes of human p450s.

关键词： Cytochrome p450 Drug metabolism Budding yeast Diclofenac Synthetic biology CORM-401 Boolean gates

Application of flame-formed carbon nanoparticle films for ethanol sensing Author links open overlay panel

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particuology 2024年第8期91卷 202-210页

作者： pegah Darvehi Luca Basta mario Commodo patrizia Minutolo Andrea D'Anna Istituto di Scienze e Tecnologie per l’Energia e la MobilitàSostenibili Consiglio Nazionale delle RicercheP.le Tecchio 8080125NapoliItaly Dipartimento di Ingegneria Chimica dei Materiali e della Produzione IndustrialeUniversitàdegli Studi di Napoli Federico IIP.le Tecchio 8080125NapoliItaly

Carbon nanoparticles(CNps)have received considerable attention due to their exceptional qualities and *** unique physical and chemical characteristics make them extremely intriguing as materials for numerous high-potential applications,such as electronics and gas *** study focused on producing carbon-based nanomaterial devices by deposition of flame-formed carbon nanoparticles on a suitable substrate and investigating their gas-sensing *** were produced in a fuel-rich laminar premixed ethylene/air flame and the collected CNp film was morphologically and electrically *** electrical conductivity of the film was investigated as a function of ethanol concentration and amount of deposited ***,CNp films exhibited high sensitivity to ambient ethanol gas concentrations,and rapid recovery times at room temperature,and showed a sensitivity increasing with the amount of deposited material and the surface *** findings demonstrate the high potential of combustion-generated CNps as building materials for low-cost and portable ethanol sensors.

关键词： Flame synthesis Carbon nanoparticles Soot Nanostructured thin film Optical and electrical characterization Atomic force microscopy Sensors

Chestnut tannin:New use,research and bioeconomy

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Journal of Bioresources and Bioproducts 2024年第3期9卷 246-252页

作者： Rosaria Ciriminna Francesco Meneguzzo Giovanna Li petri Cosimo Meneguzzo Giuseppe Angellotti mario pagliaro Istituto per lo Studio dei Materiali Nanostrutturati CNRvia U.La Malfa 15390146 PalermoItaly Istituto per la Bioeconomia CNRvia Madonna del Piano 1050019 Sesto Fiorentino(FI)Italy HyRes Srl via Salvator Rosa 1882100 BeneventoItaly

Chestnut tannin,extracted from the bark or wood of chestnut trees,possesses unique properties that make it valuable in various *** serves as a natural source of tannins,which are widely used in the production of leather,textiles,and wood *** research continues to explore its potential applications,chestnut tannin remains a promising resource with diverse industrial *** new use,research and bioeconomy aspects,this study provides a unified perspective on chestnut *** advanced applications will likely emerge shortly.

关键词： Tannin Chestnut Bioeconomy Tannin market Hydrolysable tannin

patients’dissatisfaction with multifocal intraocular lenses managed by exchange with other multifocal lenses of diferent optical profles

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Eye and Vision 2024年第1期10卷 31-40页

作者： Olena Al‑Shymali Colm McAlinden Jorge L.Alio del Barrio mario Canto‑Cerdan Jorge L.Alio Research&Development Department VISSUM Innovation and Department of Cornea Cataract and Refractive SurgeryVISSUM CorporationAlicanteSpain Department of Ophthalmology Singleton HospitalSwansea Bay University Health BoardSwanseaUK Division of Ophthalmology School of MedicineVISSUM AlicanteUniversidad Miguel HernándezCalle Cabañal 103016 AlicanteSpain Cornea Cataract and Refractive Surgery DepartmentVISSUM CorporationAlicanteSpain

Background:The aim of the study was to evaluate the outcomes of dissatisfed patients reporting poor visual quality following implantation of multifocal intraocular lenses(MF-IOLs),managed by IOL exchange with another multifocal optical ***:This is a retrospective series of ***-IOL exchange was done in 15 dissatisfed patients(30 eyes)with the perception of poor visual quality for far distance afected by neuroadaptation *** underwent a bilateral exchange of a MF-IOL with another MF-IOL of a diferent optical *** outcomes and complications were *** including Quality of Vision(QoV),Visual Function Index-14(VF-14)and its Rasch-revised version(VF-8R)and a satisfaction questionnaire were also used for outcome ***:The mean elapsed time from implantation to explantation-reimplantation was 11.8 *** QoV scores improved signifcantly across all the three *** function improved with a change in VF-14 score from 60.41±24.81 to 90.16±10.91(pproved as *** uncorrected distance visual acuity improved from 0.24 to 0.12 logMAR after exchange(pproved from 0.15 to 0.04 logMAR(ppatients’satisfaction following MF-IOL exchange,80%of the patients reported they would have the MF-IOL reimplantation procedure ***:patient dissatisfaction with neuroadaptation failure following MF-IOL implantation can be managed in 80%of our cases by MF-IOL exchange with a diferent MF-IOL optical profle.

关键词： Multifocal intraocular lenses IOL exchange Visual acuity Neuroadaptation failure

Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment: A review of their phylogeny, metabolism, and detection

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Environmental Science and Ecotechnology 2024年第5期21卷 1-16页

作者： Lucia Ruiz-Haddad Muhammad Ali mario pronk Mark C.Mvan Loosdrecht pascal E.Saikaly Environmental Science and Engineering program Biological and Environmental Science and Engineering(BESE)DivisionKing Abdullah University of Science and Technology(KAUST)Thuwal23955-6900Saudi Arabia Water Desalination and Reuse Center Biological and Environmental Science and Engineering(BESE)DivisionKing Abdullah University of Science and Technology(KAUST)Thuwal23955-6900Saudi Arabia Department of Civil Structural&Environmental EngineeringTrinity College DublinThe University of DublinDublin2Ireland Department of Biotechnology Delft University of TechnologyDelft2629 HZthe Netherlands

Currently,the most cost-effective and efficient method for phosphorus(p)removal from wastewater is enhanced biological p removal(EpBR)via polyphosphate-accumulating organisms(pAOs).This study integrates a literature review with genomic analysis to uncover the phylogenetic and metabolic diversity of the relevant pAOs for wastewater *** findings highlight significant differences in the metabolic capabilities of pAOs relevant to wastewater ***,Candidatus Dechloromonas and Candidatus Accumulibacter can synthesize polyhydroxyalkanoates,possess specific enzymes for ATp production from polyphosphate,and have electrochemical transporters for acetate and *** contrast,Tetrasphaera,Candidatus phosphoribacter,Knoellia,and phycicoccus possess polyp-glucokinase and electrochemical transporters for sugars/amino ***,this review explores various detection methods for polyphosphate and pAOs in activated sludge wastewater treatment ***,FISH-Raman spectroscopy emerges as one of the most advanced detection ***,this review provides critical insights into pAO research,underscoring the need for enhanced strategies in biological phosphorus removal.

关键词： polyphosphate-accumulating organisms(pAOs) Enhanced biological phosphorus removal (EBpR)Tetrasphaera Ca.accumulibacter Ca.phosphoribacter Knoellia phycicoccus

Antifouling properties of Electrospun polymeric Coatings Induced by Controlled Surface Morphology

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Energy & Environmental Materials 2024年第6期7卷 409-419页

作者： Fabio L.Favrin Lorenzo Zavagna Matteo Sestini Semih Esin Bahareh Azimi Massimiliano Labardi mario Milazzo Giuseppe Gallone Giovanna Batoni Serena Danti Department of Civil and Industrial Engineering University of PisaLargo Lucio Lazzarino 256122PisaItaly Department of Translational Research and New Technologies in Medicine and Surgery University of Pisavia San Zeno 35-3956123PisaItaly Institute for Chemical and physical processes(IpCF) National Research Council(CNR)Pisa Research AreaVia Moruzzi 156124PisaItaly Department of Civil and Environmental Engineering Massachusetts Institute of Technology(MIT)Massachusetts Ave.77CambridgeMassachusetts 02139USA

Nosocomial infections affect implanted medical devices and greatly challenge their functional outcomes,becoming sometimes life threatening for the ***,aggressive antibiotic therapies are administered,which often require the use of last-resort drugs,if the infection is caused by multi-drug-resistant *** the risk of bacterial contamination of medical devices in the hospitals has thus become an emerging *** routes to control these infections are based on materials provided with intrinsic bactericidal properties(i.e.,chemical action)and on the design of surface coatings able to limit bacteria adhesion and fouling phenomena(i.e.,physical action),thus preventing bacterial biofilm ***,we report the development and validation of coatings made of layer-by-layer deposition of electrospun poly(vinylidene fluoride-co-trifluoro ethylene)p(VDF-TrFE)fibers with controlled orientations,which ultimately gave rise to antifouling *** obtained 10-layer surface morphology with 90°orientation fibers was able to efficiently prevent the adhesion of bacteria,by establishing a superhydrophobic-like behavior compatible with the Cassie-Baxter ***,the results highlighted that surface wettability and bacteria adhesion could be controlled using fibers with diameter comparable to bacteria size(i.e.,achievable via electrospinning process),by tuning the intra-fiber spacing,with relevant implications in the future design of biomedical surface coatings.

关键词： additive manufacturing Escherichia coli fibers poly(vinylidene fluoride-cotrifluoro ethylene)p(VDF-TrFE) pseudomonas aeruginosa superhydrophobic

Enhanced entropy generation and heat transfer characteristics of magnetic nano-encapsulated phase change materials in latent heat thermal energy storage systems

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Applied Mathematics and Mechanics(English Edition) 2024年第6期45卷 1051-1070页

作者： p.S.REDDY p.SREEDEVI Department of Mathematics Rajeev Gandhi Memorial College of Engineering and TechnologyNandyal 518501India

The objective of the current study is to investigate the importance of entropy generation and thermal radiation on the patterns of velocity,isentropic lines,and temperature contours within a thermal energy storage device filled with magnetic nanoencapsulated phase change materials(NEpCMs).The versatile finite element method(FEM)is implemented to numerically solve the governing *** effects of various parameters,including the viscosity parameter,ranging from 1 to 3,the thermal conductivity parameter,ranging from 1 to 3,the Rayleigh parameter,ranging from 102 to 3×10^(2),the radiation number,ranging from 0.1 to 0.5,the fusion temperature,ranging from 1.0 to 1.2,the volume fraction of NEpCMs,ranging from 2%to 6%,the Stefan number,ranging from 1 to 5,the magnetic number,ranging from 0.1 to 0.5,and the irreversibility parameter,ranging from 0.1 to 0.5,are examined in detail on the temperature contours,isentropic lines,heat capacity ratio,and velocity ***,the heat transfer rates at both the cold and hot walls are analyzed,and the findings are presented *** results indicate that the time taken by the NEpCMs to transition from solid to liquid is prolonged inside the chamber region as the fusion temperatureθf ***,the contours of the heat capacity ratio Cr decrease with the increase in the Stefan number Ste.

关键词： volume fraction of nano-encapsulated phase change material(NEpCM) radiation Stefan number heat capacity ratio entropy generation

A Hybrid Optimization Approach of Single point Incremental Sheet Forming of AISI 316L Stainless Steel Using Grey Relation Analysis Coupled with principal Component Analysiss

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Journal of Wuhan University of Technology(Materials Science) 2024年第1期39卷 160-166页

作者： A Visagan p Ganesh Department of production Technology Madras Institute of TechnologyChennai-600044India

We investigated the parametric optimization on incremental sheet forming of stainless steel using Grey Relational Analysis(GRA) coupled with principal Component Analysis(pCA). AISI 316L stainless steel sheets were used to develop double wall angle pyramid with aid of tungsten carbide tool. GRA coupled with pCA was used to plan the experiment conditions. Control factors such as Tool Diameter(TD), Step Depth(SD), Bottom Wall Angle(BWA), Feed Rate(FR) and Spindle Speed(SS) on Top Wall Angle(TWA) and Top Wall Angle Surface Roughness(TWASR) have been studied. Wall angle increases with increasing tool diameter due to large contact area between tool and workpiece. As the step depth, feed rate and spindle speed increase,TWASR decreases with increasing tool diameter. As the step depth increasing, the hydrostatic stress is raised causing severe cracks in the deformed surface. Hence it was concluded that the proposed hybrid method was suitable for optimizing the factors and response.

关键词： single point incremental forming AISI 316L taguchi grey relation analysis principal component analysis surface roughness scanning electron microscopy

Electro-assisted photocatalytic reduction of CO_(2) in ambient air using Ag/TNTAs at the gas-solid interface

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Materials Reports(Energy) 2024年第2期4卷 71-82页

作者： Feng Yue Zhaoya Fan Cong Li Yang Meng Shuo Zhang Mengke Shi Minghua Wang mario Berrettoni Jun Li Hongzhong Zhang Henan Collaborative Innovation Center of Environmental pollution Control and Ecological Restoration Zhengzhou University of Light IndustryZhengzhou450001PR China Henan Institute of Advanced Technology Zhengzhou UniversityZhengzhou450052PR China Department of Chemistry University of CamerinoCamerino62032MacerataItaly

The direct conversion of atmospheric CO_(2) into fuel via photocatalysis exhibits significant practical application value in advancing the carbon *** this study,we established an electro-assisted photocatalytic system with dual compartments and interfaces,and coated Ag nanoparticles on the titanium nanotube arrays(TNTAs)by polydopamine *** the absence of sacrificial agent and alkali absorption liquid conditions,the stable,efficient and highly selective conversion of CO_(2) to CO at the gas-solid interface in ambient air was realized by photoelectric ***,with the assistance of potential,the CO formation rates reached 194.9μmol h^(−1) m^(−2) and 103.9μmol h^(−1) m^(−2) under ultraviolet and visible light irradiation,respectively;the corresponding CO_(2) conversion rates in ambient air were 30%and 16%,*** excellent catalytic effect is mainly attributed to the formation of p–N heterojunction during the catalytic process and the surface plasmon resonance ***,the introduction of solid agar electrolytes effectively inhibits the hydrogen evolution reaction and improves the electron utilization *** system promotes the development of photocatalytic technology for practical applications and provides new insights and support for the carbon cycle.

关键词： Electro-assisted photocatalytic Gas-solid interface Ambient air p–N heterojunction practical applications