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Involvement of Met receptor pathway in aggressive behavior of colorectal cancer cells induced by parathyroid hormone-related peptide

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World Journal of Gastroenterology 2022年第26期28卷 3177-3200页

作者： María Belén Novoa d.az Pedro Carriere Graciela Gigola Ariel Osvaldo Zwenger Natalia Calvo Claudia Gentili departamento de Biología Bioquímica y FarmaciaUniversidad Nacional del Sur(UNS)-INBIOSUR(CONICET-UNS)Bahía Blanca 8000Buenos AiresArgentina Centro de Estudios Clínicos SAGA CEC SAGASantiago de Chile 8320000Chile

BACKGROUNd Parathyroid hormone-related peptide(PTHrP)plays a key role in the development and progression of many *** found that in colorectal cancer(CRC)HCT116 cells,the binding of PTHrP to its receptor PTHR type 1(PTHR1)activates events associated with an aggressive *** HCT116 cell xenografts,PTHrP modulates the expression of molecular markers linked to tumor *** evidence suggests that the Met receptor is involved in the development and evolution of *** on these data,we hypothesized that the signaling pathway trigged by PTHrP could be involved in the transactivation of Met and consequently in the aggressive behavior of CRC *** To elucidate the relationship among PTHR1,PTHrP,and Met in CRC *** For in vitro assays,HCT116 and Caco-2 cells derived from human CRC were incubated in the absence or presence of PTHrP(1-34)(10-8 M).Where indicated,cells were pre-incubated with specific kinase inhibitors or dimethylsulfoxide,the vehicle of the *** protein levels were evaluated by Western blot ***-time polymerase chain reaction(RT-qPCR)was carried out to determine the changes in gene *** healing assay and morpho logical monitoring were performed to evaluate cell migration and changes related to the epithelialmesenchymal transition(EMT),*** number of viable HCT116 cells was counted by trypan blue dye exclusion test to evaluate the effects of irinotecan(CPT-11),oxaliplatin(OXA),or doxorubicin(dOXO)with or without *** in vivo tests,HCT116 cell xenografts on 6-wk-old male N:NIH(S)_nu mice received daily intratumoral injections of PTHrP(40μg/kg)in 100μL phosphate-buffered saline(PBS)or the vehicle(PBS)as a control during 20 *** slaughter was carried out and the tumors were removed,weighed,and fixed in a 4%formaldehyde solution for subsequent treatment by *** evaluate the expression of molecular markers in human tumor samples,we studied 23 specimens ob

关键词： PTHrP MET receptor tyrosine kinase Parathyroid hormone receptor type 1 Colorectal cancer drug resistance

Involvement of parathyroid hormone-related peptide in the aggressive phenotype of colorectal cancer cells

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World Journal of Gastroenterology 2021年第41期27卷 7025-7040页

作者： María Belén Novoa d.az Pedro Matías Carriere María Julia Martín Natalia Calvo Claudia Gentili departamento de Biología Bioquímica y FarmaciaUniversidad Nacional del Sur(UNS)-INBIOSUR(CONICET-UNS)Bahía Blanca 8000Buenos AiresArgentina departamento de Química Universidad Nacional del Sur(UNS)-INQUISUR(CONICET-UNS)Bahía Blanca 8000Buenos AiresArgentina

Colorectal cancer(CRC)remains one of the leading causes of mortality from malignant diseases *** general terms,CRC presents high heterogeneity due to the influence of different genetic and environmental factors;also,the neoplastic cells are strongly influenced by the extracellular matrix and several surrounding cells,known together as the tumor microenvironment(TME).Bidirectional communication takes place between the tumor and the TME through the release of autocrine and paracrine *** hormone-related peptide(PTHrP)is a cytokine secreted by a wide variety of tissues and is able to regulate several cellular functions both in physiological as well as in pathological *** exerts its effects as a paracrine/autocrine factor,although its mode of action is mainly *** has been shown that this peptide is expressed by several tumors and that the tumor secretion of PTHrP is responsible for the malignant humoral *** years ago,when our research group started studying PTHrP effects in the experimental models derived from intestinal tumors,the literature available at the time addressing the effects of PTHrP on colorectal tumors was limited,and no articles had been published regarding to the paracrine action of PTHrP in CRC *** on this and on our previous findings regarding the role of PTH in CRC cells,our purpose in recent years has been to explore the role of PTHrP in *** analyzed the behavior of CRC cells treated with exogenous PTHrP,focalizing in the study of the following events:Survival,cell cycle progression and proliferation,migration,chemoresistance,tumor-associated angiogenesis,epithelial to mesenchymal transition program and other events also associated with invasion,such us the induction of cancer stem cells *** work summarizes the major findings obtained by our investigation group using in vitro and in vivo CRC models that evidence the participation of PTHrP in the acquisition of an aggressiv

关键词： Parathyroid hormone-related protein Colorectal cancer Tumor biomarkers Neoplastic processes drug resistance Tumor microenvironment

Survival effect of probiotics in a rat model of colorectal cancer treated with capecitabine

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World Journal of Gastrointestinal Oncology 2021年第10期13卷 1518-1531页

作者： Graciela Gigola Pedro Carriere María Belén Novoa d.az Gabriela Perdigon Ariel Osvaldo Zwenger Claudia Gentili Biología Bioquímica y FarmaciaUniversidad Nacional del Sur(UNS)-INBIOSUR(CONICET-UNS)Bahía Blanca 8000Buenos Aires ProvinciaArgentina San Miguel de Tucumán 4000Argentina Centro de Estudios Clínicos SAGA CEC SAGASantiago de Chile 8320000Chile

BACKGROUNd Probiotics are used to manage a number of gastrointestinal disorders due to their beneficial *** reports showed that probiotics also improve the life quality of patients with colorectal cancer(CRC)subjected to oncologic *** a CRC animal model,probiotics supplementation has the potential to decrease the formation of aberrant crypts and ameliorate tumor malignancy,enhancing the antitumor effect of 5-fluorouracil(5-FU)*** on these data,we hypothesize that the administration of probiotics impact positively in the overall survival and life quality of rats with CRC under the treatment of capecitabine,which is the pro drug of *** To evaluate the probiotics effects in a rat CRC model treated with capecitabine and followed until the end of *** 1,2-dimethylhid.azine dihydrochloride(1,2-dMH)was employed as carcinogen inductor of *** male Wistar-Lewis rats were randomly assigned to one of five following groups:Control(n=5),Control+probiotics(Control-P group,n=5),1,2-dMH alone(dMH group,n=10),1,2-dMH+capecitabine(dMH-C group,n=10),1,2-dMH+probiotics(dMH-P group,n=10)and 1,2-dMH+capecitabine+probiotics(dMH-C-P group,n=10).All parametric data were expressed as the mean±*** statistical significance of differences was analyzed using one-way *** were analyzed with InfoStat *** results were considered statistically significant at Pd with the Kaplan-Meier estimator with the log-rank *** The data of mean overall survival for dMH,dMH-P,dMH-C,dMH-C-P,Control and Control-P groups were 250 d[95%confidence interval(CI):242.5-253.1],268 d(95%CI:246.3-271.4),380 d(95%CI:337.8-421.9),480 d(95%CI:436.9-530.7),588 d(95%CI:565.8-609.3)and 590 d(95%CI:564.3-612.9),respectively,with a significant difference between dMH-C and dMH-C-P groups(P=0.001).Comparing all groups by Kaplan-Meier estimator,we found a significantly different in the overall survival of dMH and dMH-

关键词： Colorectal cancer Capecitabine Probiotics Survival Life quality Animal experimentation

Biochar from grape pomace,a waste of vitivinicultural origin,is effective for root-knot nematode control

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Biochar 2023年第1期5卷 519-541页

作者： Ángela Martínez-Gómez Maria Fe Andrés Ángel Barón-Sola Fernando E.d.az-Manzano Ibraheem Yousef Ismael F.Mena Elena d.az Óscar Gómez-Torres azucena González-Coloma Luis E.Hernández Carolina Escobar Facultad de Ciencias Ambientales y Bioquímica Universidad de Castilla-La ManchaToledoSpain departamento de Protección Vegetal Instituto Ciencias Agrarias CSICMadridSpain departamento de Biología Universidad Autónoma de MadridMadridSpain CELLS ALBA Carrer del Llum 2-2608290Cerdanyola del VallèsBarcelonaSpain departamento de Ingeniería Química Universidad Autónoma de MadridMadridSpain Faculty of Chemical Sciences&Technologies Chemical Engineering DepartmentAv.Camilo JoséCela1213071University of Castilla-La ManchaCiudad RealSpain

Root-knot nematodes(RKNs)are obligate endoparasites that feed on their host plants to complete its life cycle,representing a major threat to agriculture and economy *** development of new management strategies becomes essential as effective chemical nematicides are progressively being ***,we analysed grape pomace-derived biochars,pyrolysed at 350℃(BC350)and 700℃(BC700),focusing on their potential for RKN *** thermal treatment of grape pomace caused an increase in the concentration of carbon and plant macro-and micronutrients,which were largely present in a water-soluble *** radiation-based Fourier transform infrared microspectroscopy data showed a general loss of carboxylic functional groups during pyrolysis,partially contributing to the alkalinisation of both biochars,mostly in *** electron microscopy coupled with energydispersive X-ray spectroscopy analysis revealed a highly porous structure filled with different crystals composed of elements such as K,Ca,Mg,P,Si or Al,which could be a suitable environment for the growth of ***-derived aqueous extracts showed phytotoxicity to tomato seedlings at high concentrations,and disappeared upon dilution,but no toxic effect was observed on the nematode’s infective ***,the infective and reproductive traits of a Meloidogyne javanica population in tomato were significantly reduced(*** masses and eggs per plant)in washed-biochar-treated soil in pots(0.75%;BC350W).Therefore,the large amount of grape waste generated after wine production can be transformed into a valuable product such as biochar,effective for RKNs control,thus reducing the waste management problem and contributing to a circular economy.

关键词： Biochar Grape pomace Tomato Root-knot nematode control Meloidogyne spp.

A brief history of the Compendium of Physical Activities

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Journal of Sport and Health Science 2024年第1期13卷 3-5页

作者： Barbara E.Ainsworth Stephen d.Herrmann david R.Jacobs Jr. Melicia C.Whitt-Glover Catrine Tudor-Locke School of Exercise and Health Shanghai University of SportShanghai 200438China College of Health Solutions Arizona State UniversityPhoenixAZ 85003USA Kansas Center for Metabolism and Obesity Research University of Kansas Medical CenterKansas CityKS 66160USA division of Epidemiology and Community Health University of MinnesotaMinneapolisMN 55455USA Gramercy Research Group KnightdaleNC 27545USA College of Health and Human Services University of North Carolina at CharlotteCharlotteNC 28223USA

The Compendium of Physical Activities(Compendium)was developed to address consistent assignment of physical activity(PA)intensity values used in PA epidemiology research of the association between PA and health outcomes.1The known protective effects of PA on incident health outcomes traces to the mid-1900s,with over 50 studies examining coronary heart disease(CHd)as the outcome of interest.

关键词： epidemiology Activities protective

Graphene-calcium carbonate coating to improve the degradation resistance and mechanical integrity of a biodegradable implant

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Journal of Magnesium and Alloys 2024年第1期12卷 394-404页

作者： Lokesh Choudhary Parama Chakraborty Banerjee R.K.Singh Raman derrek E.Lobo Christopher d.Easton Mainak Majumder Frank Witte Jörg F.Löffler department of Mechanical and Aerospace Engineering Monash UniversityClayton CampusMelbourneVIC 3800Australia department of Chemical and Biological Engineering Monash UniversityClayton CampusMelbourneVIC 3800Australia Nanoscale Science and Engineering Laboratory(NSEL) Bldg 37Monash UniversityClayton CampusMelbourneVIC 3800Australia CSIRO Materials Science and Engineering Bayview AvenueClaytonVIC 3168Australia Julius Wolff Institute and Berlin Brandenburg Center for Regenerative Therapies Charité–Universitätsmedizin BerlinAugustenburger Platz 113353 BerlinGermany Laboratory of Metal Physics and Technology Department of MaterialsETH Zurich8093 ZurichSwitzerland

Biodegradable implants are critical for regenerative orthopaedic procedures,but they may suffer from too fast corrosion in human-body *** necessitates the synthesis of a suitable coating that may improve the corrosion resistance of these implants without compromising their mechanical *** this study,an az91 magnesium alloy,as a representative for a biodegradable Mg implant material,was modified with a thin reduced graphene oxide(RGO)-calcium carbonate(CaCO_(3))composite *** analytical and in-vitro electrochemical characterization reveals that this coating significantly improves the corrosion resistance and mechanical integrity,and thus has the potential to greatly extend the related application field.

关键词： Graphene coating Biodegradable implant Hydroxyapatite Corrosion Magnesium alloy

Preliminary design for Station-Keeping of a Communication Satellite

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Computer Aided drafting,design and Manufacturing 2010年第2期20卷 68-77页

作者： LENIN A Luna d.az School of Astronautics Beijing University of Aeronautics and Astronautics

This paper has analyzed the perturbations of a GeoX-Sat （virtual satellite） in geostationary orbit （GEO）. And control methods, strategies and calculation processes are designed of the East-West （E/W） station-keeping of the GeoX-Sat. The Attitude and Orbit Control Subsystem （AOCS） provides attitude information and maintains the required spacecraft attitude during all phases of the mission, starting at spacecraft separation from the launch vehicle and throughout its operational lifetime. For the GeoX-Sat exercising design, due to time limitations; only two modes of operation were considered after assumed launching operation： normal and station keeping.

关键词： communication satellite AOCS East/West station keeping

A comparison of process performance during the anaerobic mono-and co-digestion of slaughterhouse waste through different operational modes

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Journal of Environmental Sciences 2018年第2期30卷 149-156页

作者： Jhosané Pagés-d.az Ileana Pereda-Reyes Jose Luis Sanz Magnus Lundin Mohammad J.Taherzadeh Ilona Sárvári Horváth Process Engineering Centre (CIPRO) Universidad Tecnológica de La Habana "José Antonio Echeverría" (Technical University of Havana "José A. Echeverría") (Cujae) Swedish Centre for Resource Recovery University of Bor?s department of Molecular Biology Autonomous University of Madrid

The use of consecutive feeding was applied to investigate the response of the microbial biomass to a second addition of substrates in terms of biodegradation using batch tests as a promising alternative to predict the behavior of the process. Anaerobic digestion（Ad） of the slaughterhouse waste（SB） and its co-digestion with manure（M）, various crops（VC）, and municipal solid waste were evaluated. The results were then correlated to previous findings obtained by the authors for similar mixtures in batch and semi-continuous operation modes. Ad of the SB failed showing total inhibition after a second feeding. Co-digestion of the SB ＋ M showed a significant improvement for all of the response variables investigated after the second feeding, while co-digestion of the SB ＋ VC resulted in a decline in all of these response variables. Similar patterns were previously detected, during both the batch and the semi-continuous modes.

关键词： Co-digestion Biomethane potential test Second feeding Specific methanogenic activity Slaughterhouse waste

Photooxidative stress activates a complex multigenic response integrating the phenylpropanoid pathway and ethylene,leading to lignin accumulation in apple(Malus domestica Borkh.)fruit

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Horticulture Research 2020年第1期7卷 2501-2513页

作者： Carolina A.Torres Constanza azocar Patricio Ramos Ricardo Pérez-d.az Gloria Sepulveda María A.Moya-León Facultad de Ciencias Agrarias Universidad de TalcaTalcaChile department of Horticulture Tree Fruit Research&Extension CenterWashington State UniversityWenatcheeWAUSA Universidad Andres Bello Facultad Ciencias BiologicasSantiagoChile Instituto de Ciencias Biológicas Universidad de TalcaTalcaChile Núcleo Cientifíco Multidisciplinario-dI Universidad de TalcaTalcaChile

Photooxidative stress,when combined with elevated temperatures,triggers various defense mechanisms leading to physiological,biochemical,and morphological changes in fruit ***,during sun damage,apple fruit undergo textural changes characterized by high flesh firmness compared to unexposed *** and Royal Gala apples were suddenly exposed to sunlight on the tree and then sampled for up to 29 *** wall components and lignin biosynthetic pathway analyses were carried out on the fruit *** harvest,Fuji apples with different sun exposure levels,such as exposed to direct sunlight(Exp),shaded(Non-Exp),and with severe sun damage(Sev),were also *** fruit suddenly exposed to sunlight,the expression levels of phenylpropanoid-related genes,phenylalanine ammonia lyase(MdPAL),chalcone synthase(MdCHS),and flavanone-3-hydroxylase(MdF3H),were upregulated in the skin and flesh of Exp and *** had little effect on the lignin-related genes caffeic acid Omethyltransferase 1(MdCOMT1)and cinnamyl alcohol dehydrogenase(MdCAd)in the skin;however,the expression of these genes was highly induced in the flesh of Exp and Sev in both *** deposition increased significantly in skin with sun injury(Sev);in flesh,this increase occurred late during the stress ***,the ethylene biosynthesis genes 1-aminocyclopropane-1-carboxylate synthase(MdACS)and 1-aminocyclopropane-1-carboxylate oxidase(MdACO)were highly expressed in the skin and flesh tissues but were more upregulated in Sev than in Exp during the time-course experiment,which paralleled the induction of the phenylpropanoid pathway and lignin *** harvest,flesh from Sev fruit exhibited higher firmness than that from Non-Exp and Exp fruit,although no differences were observed in the alcohol-insoluble residues(AIR)among *** fractionation of cell wall polymers revealed an increase in the uronic acid contents of the water-soluble pectin fraction(WSF)in Ex

关键词： stress involvement suddenly