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Wilt Disease of Tara (Caesalpinia spinoseMolina Kuntz) in Nursery in the Inter Andean Valley of Cochabamba, Bolivia

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American Journal of Plant Sciences 2018年第9期9卷 1963-1973页

作者： mario coca morante Escuela de Ciencias Forestales (ESFOR) Departamento de Fitotecnia Facultad de Ciencias Agrícolas y Pecuarias (FCAyP) “Martin Cárdenas” Universidad Mayor de San Simón Cochabamba Bolivia

Tara (Caesalpinia spinose Molina Kuntz) is a native plant of the Bolivian Andes. A number of projects have promoted the cultivation of this species over the last 20 years, in efforts to make use of its medicinal, food and industrial properties. However, as tara plantations have grown, so too has the incidence of disease affecting the species, leading to economic losses. The aim of the present work was to identify the causal agent of wilting in tara, and to determine the incidence of the disease in nursery-grown plants. Three experimental plots (R1-R3) were established in plantlet production bedsat the ESFOR nursery in order to assess the incidence of wilting. Plant spacing was 1 × 1 m. At the two leaf stage, samples of diseased and apparently healthy plants were taken to the laboratory for the isolation of pathogens. Both the apparently healthy and diseased plants gave rise to the same kinds of fungal colony on PDA. Microscopic examination revealed microconidia, macroconidia, phialides and chlamydospores suggesting the causal agent to be Fusarium oxysporum. Over the (up to) 84-day period following thinning, the incidence of wilting for 2010 was R1 = 2.56%, R2 = 3.15% and R3 = 2.22% per 14 days, and for 2013 it was R1 = 1.24%, R2 = 2.45% and R3 = 2.13% per 14 days;the apparent infection rates for 2010 were rR1 = 0.0003/day, rR2 = 0.0003/day and rR3 = 0.0003/day, and for 2013 they were rR1 = 0.0003/day, rR2 = 0.0004/day and rR3 = 0.0003/day. These values are characteristic of monocyclic epiphytic fungi such as F. oxysporum.

关键词： Soil Pathogens Monocyclic Epiphytic Fungi Disease Intensity Fusarium oxysporum

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Wild potato species (Solanumsection Petota Solanaceae) in the Tunari National Park, Andean Region of Cochabamba, Bolivia

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Open Journal of Ecology 2014年第2期4卷 28-32页

作者： mario coca morante Alejandro coca-Salazar Centro de Biodiversidad y Genética Facultad de Ciencias y Tecnología Universidad Mayor de San Simón Cochabamba Bolivia Departamento de Fitotecnia y Producción Vegetal Facultad de Ciencias Agrícolas Pecuarias Forestales y Veterinarias “Martín Cárdenas” Universidad Mayor de San Simón Cochabamba Bolivia

The Tunari National Park is located in the Tunari Mountain Range, part of the Cochabamba Range in the Bolivian Andes. The Park is home to species of wild potato (Solanum section Petota Solanaceae), but these are now threatened by human activity. The aims of the present study were: 1) to determine the distribution of wild potato species in the Park, and, 2) to determine the size of their populations. Collection routes were established within the Park, and the species present along them recorded in 2006, 2007 and 2008. Their population sizes were determined in 2008. Four wild potato species were identified: S. capsicibaccatum, S. berthaultii, S. brevicaule and S. toralapanum. S. capsicibaccatum was the most abundant and had the most extensive distribution, followed by S. berthaultii;these last two species are likely endemic. The other species, although probably also endemic, were much less widely distributed;some limiting factor(s) would therefore appear to be acting upon them. S. berthaultii showed variations in corolla shape (rotate, pentagonal and semi-stellate) and flower colour (whitish-lilac to blue-lilac). The remaining species showed no apparent intraspecific variation in their morphological characteristics.

关键词： Genetic Erosion Anthropic Activity Biological Diversity

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The Potato Late Blight Caused by Phytophthora infestansMont de Bary as Selection Factor of Phurejas Potatoes (Solanum phurejaJuz et Buk) in Endemic Areas of the Bolivian Andes

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American Journal of Plant Sciences 2013年第1期4卷 53-58页

作者： mario coca-morante Ismael Tolín-Tordoya Departamento de Fitotecnia y Producción Vegetal Facultad de Ciencias Agrícolas Pecuarias Forestales y Veterinarias “Dr. Martin Cárdenas” Universidad Mayor de San Simón Cochabamba Bolivia Programa de Asistencia a la Seguridad Alimentaria La Paz Bolivia

Phurejas potatoes (Solanum phureja Juz et Buk) are cultivated in very restricted areas of the Bolivian Andes. Late blight caused by Phytophthora infestans is a destructive, endemic disease that affects the survival and cultivation of this crop. The aim of this study was to determine the resistance characteristics of phurejas potato varieties to P. infestans in a traditional area of cultivation. An experimental plot was prepared in the locality of Chojchoni (3200 m) and planted with different phurejas varieties: 1) Chojllu Phureja (Sste);2) Phureja Amarilla (Sphu);3) Polo Phureja (Sphu);4) Phureja Roja (Sphu);5) Phureja Negra (Sphu) and 6) Waych’a Pacena (Sadg), a susceptible control. Severity was assessed according to the criteria of the Centro Internacional dela Papa, determining the area under the disease progress curve (AUDPC). Regression analysis (logN disease severity against time) was performed using the logistic model [ln(y/1 - y)] to determine infection rates. The different varieties showed different levels of resistance. The disease progress curve, the AUDPC and the linear regression results revealed the varieties Chojllu Phureja, Phureja Amarilla and Polo Phureja to likely have larger copy numbers of minor resistance genes against P. infestans than Phureja Roja or Phureja Negra. Late blight caused by P. infestans could cause the long-term loss of some phurejas varieties through farmers selecting others less severely affected by the disease.

关键词： Apparent Infection Rate Genetic Erosion Late Blight

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Reassessing the AMPK-MTORC1 balance in autophagy in the central nervous system

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Neural Regeneration Research 2025年第11期20卷 3209-3210页

作者： Marta García-Juan mario Villa Irene Benito-Cuesta Lara Ordónez-Gutiérrez Francisco Wandosell Centro de Biología Molecular“Severo Ochoa”(CSIC-UAM) Nicolas Cabrera 1Universidad Autónoma de MadridMadridSpain Fisiología Animal Departamento de BiologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Department of Clinical Neuroscience CMM Karolinska Universitetssjukhuset SolnaStockholmSweden Departamento de Bioquímica y Biología Molecular Universidad Complutense de MadridMadridSpain Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas(CIBERNED) MadridSpain

Autophagy is a cellular degradation and recycling system,indispensable for cellular and organ development,homeostasis,and *** cellular process is evolutionarily highly conserved to quality control of many proteins and dysfunctional organelles,which finally recycle components as amino *** process is effective during normal physiology as part of anabolism and plays an additional important role during starvation(Dikic and Elazar,2018).Different types of autophagy have been chara cterized based on their dynamic,mechanism of action,target substrates,and protein *** of them are macroautophagy(hereafter called"autophagy"),microa utophagy,and chape rone-media ted autophagy(Fleming et al.,2022).

关键词： homeostasis system hereafter

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Control of Leaf Spot Diseases on Ecotypes of Faba Bean (Vicia fabaL.) Produced in the Andean Region of Bolivia

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American Journal of Plant Sciences 2012年第8期3卷 1150-1158页

作者： M. coca-morante F. Mamani-álvarez Departamento de Fitotecnia y Producción Vegetal Facultad de Ciencias Agrícolas Pecuarias Forestales y Veterinarias Dr. “Martín Cárdenas” Universidad Mayor de San Simón (UMSS) Cochabamba Bolivia 2Instituto Nacional de Investigación Agrícola y Forestal (INIAF) La Paz Bolivia

The basin of Lake Titicaca is a faba bean-producing microregion of Bolivia where the crop is destined for export. The most commonly cultivated ecotypes “Gigante de Copacabana” and “Usnayo” are affected by diseases that can cause production losses. The aims of the present work were to identify the causal agents of leaf spot affecting these ecotypes, to record disease intensity levels, and to estimate their effect on production. In 2004 and 2005, leaflet, stem and pod samples were taken from faba bean plants with leaf spot growing in the Lake Titicaca area, and from plants in an experimental plot established to determine the effect of five different treatments on production and disease intensity: T1 = Control;T2 = seed treatment with Trichoderma sp. + alternate foliar spraying with benomyl and mancozeb;T3 = seed treatment with fludioxanil/metalaxyl M + alternate foliar spraying with benomyl and mancozeb;T4 = foliar spraying with Trichoderma sp.;T5 = alternate foliar spraying with cymoxanyl and mancozeb-chlorothalonil. Microscopic analysis of the samples revealed the presence of new fungal pathogens for faba beans in Bolivia (Cladosporium sp., and Lepthosphaerulina sp.) as well as emerging fungal pathogens (Botrytis cinerea, B. fabae, Ascochyta fabae, Alternaria sp. and Cercospora sp.). None of the treatments affected the disease progression curve (DPC) for incidence, although effects were seen with respect to disease severity. The seed + foliar treatments (T2 and T3) were more effective at controlling disease than leaf treatments on their own (T4 and T5). Modelling analysis showed faba bean leaf spot disease to be moderately destructive. Compared to chemical treatments, biocontrol with Trichoderma spp. preliminary was found to provide good control of the disease. Losses due to leaf spot disease of 36% were recorded, and a strong correlation detected between yield and disease severity.

关键词： Ecotypes Modeling Diseases Intensity

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Non-exposed endoscopic wall-inversion surgery with one-step nucleic acid amplification for early gastrointestinal tumors:Personal experience and literature review

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World Journal of Gastroenterology 2023年第24期29卷 3883-3898页

作者： Francesco Crafa Serafino Vanella Aristide morante Onofrio A Catalano Kelsey L Pomykala mario Baiamonte Maria Godas Alexandra Antunes Joaquim Costa Pereira Valentina Giaccaglia Division of General and Surgical Oncology St.Giuseppe Moscati HospitalCenter of National Excellence and High SpecialtyAvellino 83100Italy Division of Gastorenterology and Endoscopy St.Giuseppe Moscati HospitalCenter of National Excellence and High SpecialtyAvellino 83100Italy Department of Radiology Athinoula A Martinos Center for Biomedical ImagingMassachusetts General HospitalHarvard Medical SchoolBostonMA 02114United States Department of Nuclear Medicine Department of Radiological SciencesUniversity Hospital EssenUniversity of Duisburg-EssenEssen 45141Germany Department of General Surgery Braga HospitalBraga 4710-243Portugal Department of Surgery Medclinic City HospitalDubai 505004United Arab Emirates

BACKGROUND Laparoscopic and endoscopic cooperative surgery is a safe,organ-sparing surgery that achieves full-thickness resection with adequate *** studies have demonstrated the safety and efficacy of these ***,these techniques are limited by the exposure of the tumor and mucosa to the peritoneal cavity,which could lead to viable cancer cell seeding and the spillage of gastric juice or enteric liquids into the peritoneal ***-exposed endoscopic wallinversion surgery(NEWS)is highly accurate in determining the resection margins to prevent intraperitoneal contamination because the tumor is inverted into the visceral lumen instead of the peritoneal *** intraoperative assessment of the nodal status could allow stratification of the extent of ***-step nucleic acid amplification(OSNA)can provide a rapid method of evaluating nodal tissue,whilst nearinfrared laparoscopy together with indocyanine green can identify relevant nodal tissue *** To determine the safety and feasibility of NEWS in early gastric and colon cancers and of adding rapid intraoperative lymph node(LN)assessment with *** The patient-based experiential portion of our investigations was conducted at the General and Oncological Surgery Unit of the *** Moscati Hospital(Avellino,Italy).Patients with early-stage gastric or colon cancer(diagnosed via endoscopy,endoscopic ultrasound,and computed tomography)were *** lesions were treated by NEWS procedure with intraoperative OSNA assay between January 2022 and October *** were examined intraoperatively with OSNA and postoperatively with conventional *** analyzed patient demographics,lesion features,histopathological diagnoses,R0 resection(negative margins)status,adverse events,and follow-up *** were collected prospectively and analyzed *** A total of 10 patients(5 males and 5 females)with an average age of 70.4±4.5 years(range:62

关键词： Laparoscopic and endoscopic cooperative surgery Non-exposed endoscopic wall inversion surgery Early gastric cancer Early colorectal cancer Sentinel lymph node One-step nucleic acid amplification Endoscopic full-thickness resection

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作者： Elsa morante

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Plant-herbivore assemblages under natural conditions are driven by plant size,not chemical defenses

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Journal of Plant Ecology 2017年第6期10卷 1012-1021页

作者： Eva Castells Maria morante Sandra Saura-Mas Anabel Blasco-Moreno Departament de Farmacologia Terapèutica i ToxicologiaUniversitat Autònoma de BarcelonaCerdanyola del Vallès 08193Spain CREAF Cerdanyola del Vallès 08193Spain Department de Biologia Animal Vegetal i EcologiaUniversitat Autònoma de BarcelonaCerdanyola del Vallès 08193Spain Servei d’Estadística Aplicada Universitat Autònoma de BarcelonaCerdanyola del Vallès 08193Spain

Aims Plant secondary metabolites have been traditionally recognized as key traits regulating plant-herbivore ***,the ecological relevance of secondary metabolites as resistance mecha-nisms in comparison to other plant attributes,including physical,morphological or ecological traits,has been recently *** aim to evaluate the role of chemical defenses,plant size and the presence of insect competitors on driving the differences in her-bivory damage under natural *** We performed a replicated field study on the herbivore commu-nity associated with four Senecio species(***,***,*** and ***)during a full-reproductive season in Montseny Natural Park(catalonia,NE Spain).Pyrrolizidine alka-loids(PAs),the most characteristic chemical defenses of Senecio due to their toxic effects on herbivores,were analyzed by gas *** plant size was estimated by the number of flower heads produced over the entire reproductive *** used linear mixed models to explore the relationships between total PA concentrations,plant size and herbivory *** Findings PA concentrations were not related to the natural guild of herbivores within any plant species or insect ***,no significant interactions were found between insect species sharing the same host *** contrast,herbivore abundance was positively related to plant size in ***,*** and *** found no evidence that PAs confer an increased plant resistance against herbivores in *** study supports the hypothesis that plant chemical defenses have a secondary role in determining plant-her-bivore assemblages in comparison to other plant traits under the complexity of natural conditions.

关键词： Senecio herbivory chemical defenses pyrrolizidine alkaloids plant traits

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A digital earth platform for sustainability

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International Journal of Digital Earth 2017年第4期10卷 342-355页

作者： mario Hernandez Engagement Committee Future EarthMexico CityMexico International Society for Digital Earth Mexico CityMexico

Based on the experience of the International Society for Digital Earth(ISDE),this paper describes some challenges foreseen in order to develop a Digital Earth platform that can support the implementation of the Sustainable Development *** use of ready-to-use derived geospatial information is *** Earth’s methodology of‘co-design’aims to bring together natural,social scientists and decision makers to plan and carry out research for *** implies transdisciplinary research,but in order for scientists of different disciplines to work together,they will need to be able to share,access and use common *** is by far not simple!While the good will to share data might exist,the associated technological,ethical and privacy issues are difficult to *** adequate e-infrastructure will be *** could consider to use the SDGs is the basis to develop the desired Digital Earth *** paper,by no means,covers everything for a Digital Earth platform,it aims to trigger research discussions and to have a good view about a starting point.

关键词： Digital earth platform sustainable development Sustainable Development Goals future earth

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PROJECT REGULARITY： DEVELOPMENT AND EVALUATION OF A NEW PROJECT CHARACTERISTIC

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Journal of Systems Science and Systems Engineering 2017年第1期26卷 100-120页

作者： Jordy Batselier mario Vanhoucke Faculty of Economics and Business Administration Ghent Universit)/ Tweekerkenstraat 2 9000 Ghent Belgium Technology and Operations Management Area Vlerick Business School Reep 1 9000 Ghent Belgium UCL School of Management University College London Gower Street London WC1E 6BT United Kingdom

The ability to accurately characterize projects novel project characteristic is developed characteristic, called project regularity, is essential to good project management. Therefore, a reflects the value accrue within a project. This expressed in terms of the newly introduced regular/irregular-indicator RI. The widely accepted management system of earned value management （EVM） forms the basis for evaluation of the new characteristic. More concretely, the influence of project regularity on EVMforecasting accuracy is assessed, and is shown to be significant for both time and cost forecasting. Moreover, this effect appears to be stronger than that of the widely used characteristic of project seriality expressed by the serial/parallel-indicator SP. Therefore, project regularity could also be useful as an input parameter for project network generators. Furthermore, the introduction of project regularity can provide project managers with a more accurate indication of the time and cost forecasting accuracy that is to be expected for a certain project and, correspondingly, of how a project should be built up in order to obtain more reliable forecasts during project control.

关键词： Project management earned value management time and cost forecasting empirical database Monte Carlo simulation project control system

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