检索结果-南通市图书馆

Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity

引用

Journal of Animal Science and Biotechnology 2017年第4期8卷 852-865页

作者： Moana Rodrigues Franca Maressa Izabel Santos da Silva Guilherme Pugliesi Veerle Van Hoeck mario binelli Department of Animal Reproduction School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo225Duque de Caxias Norte Ave.Jd.Elite13635-900 PirassunungaSPBrazil Gamete Research Centre University of AntwerpAntwerpBelgium

Background: In beef cattle, changes in the periovulatory endocrine milieu are associated with fertility and conceptus growth. A large preovulatory follicle(POF) and the resulting elevated concentrations of progesterone(P4) during diestrus positively affect pregnancy rates. Amino acids(AA) are important components of maternally derived secretions that are crucial for embryonic survival before implantation. The hypothesis is that the size of the POF and the concentration of P4 in early diestrus modulate the endometrial abundance of SLC transcripts related to AA transport and metabolism and subsequently impact luminal concentrations of AA. The follicle growth of Nelore cows was manipulated to produce two experimental groups: large POF and CL(LF-LCL group) and small POF and CL(SF-SCL group). On Day 4(D4; Experiment 1) and Day 7(D7; Experiment 2) after Gn RH-induced ovulation(Gn RH treatment = D0), the animals were slaughtered and uterine tissues and uterine washings were collected. q RT-PCR was used to evaluate the expression levels of AA transporters in D4 and D7 endometrial *** concentrations of AA were quantified in D4 and D7 uterine washings by ***: Transcript results show that, on D4, SLC6 A6, SLC7 A4, SLC17 A5, SLC38 A1, SLC38 A7 and SCLY and on D7 SLC1 A4, SLC6 A1, SLC6 A14, SLC7 A4, SLC7 A7, SLC7 A8, SLC17 A5, SLC38 A1, SLC38 A7, SLC43 A2 and DDO were more abundant in the endometria of cows from the LF-LCL group(P < 0.05). In addition, concentrations of AA in the uterine lumen were influenced by the endocrine profiles of the mother. In this context, D4 uterine washings revealed that greater concentrations of taurine, alanine and α-aminobutyric acid were present in SF-SCL(P < 0.05).In contrast, lower concentrations of valine and cystathionine were quantified on D7 uterine washings from SF-SCL cows(P < 0.05).Conclusion: The present study revealed an association between the abundance of transcripts related to AA transport and metabolism in the endo

关键词： Amino acids Beef cattle Sex steroids Uterus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Calcium potentiates the effect of estradiol on PGF2α production in the bovine endometrium

引用

Journal of Animal Science and Biotechnology 2015年第2期6卷 215-221页

作者： Claudia Maria Bertan Membrive Pauline Martins da Cunha Flávio Vieira Meirelles mario binelli S?o Paulo State University Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S?o Paulo Department of Veterinary Medicine School of Animal Sciences and Food Engineering University of S?o Paulo

Background： Estradiol （E2） is required for luteolysis in cows and its injection stimulates prostaglandin F2α （PGF2α） release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore （CI） A23187 to synthesize PGF2α. Results： Treatment with E2 in vivo resulted in a 48.4% increase of PGF2α production by endometrial explants treated in vitro with A23187. Production of PGF20 was better stimulated with A23187 at concentrations of 10^-6 and 10^-5 mol/L compared with other concentrations used. The concentration of PGF2α for untreated bovine endometrial cell cultures was 33.1 pg/mL, while for cultures treated with E2, A23187, or a combination of E2 and A23187, the PGF2α concentration was 32.5, 92.4 and 145.6 pg/mL, respectively. Conclusions： Treatment with A23187 tended to stimulate PGF20 production. In the presence of E2, A23187 significantly stimulated PGF20 synthesis. It appears that A23187 potentiates the effects of E2 with respect to synthesis of endometrial PGF2α in cattle.

关键词： Animal reproduction Cattle Estradiol Luteolysis PGF2α synthesis Reproductive physioloqy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle

引用

Journal of Animal Science and Biotechnology 2019年第1期10卷 56-66页

作者： Thiago Martins Guilherme Pugliesi Mariana Sponchiado Angela M.Gonella-Diaza Oscar A.Ojeda-Rojas Frederich D.Rodriguez Roney S.Ramos Andrea C.Basso mario binelli Department of Animal Reproduction School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo Department of Nutrition and Animal Production School of Veterinary Medicine and Animal ScienceUniversity of Sao Paulo University Corporation of Huila ABS Global In Vitro Brasil Department of Animal Sciences University of Florida

Background: A major,unresolved issue is how the uterine microenvironment determines pregnancy success in *** implantation,conceptus development depends on the uterine secretome(i.e.,histotroph).Despite its pivotal role,little is known about the dynamics of histotroph synthesis and changes in composition throughout the early diestrus and the relevance to pregnancy *** hypothesize that disturbances on histotroph composition affect the establishment of *** was to disturb histotroph composition at early diestrus and verify the effects on:(Exp.1) timing to restore its composition; and(Exp.2) pregnancy rate after multiple-embryo *** cycle of multiparous Nelore cows were synchronized and estrus was considered d 0(D0) of the *** was through flushing each uterine horn with 30 m L of DMPBS and collecting the resulting uterine luminal flushing(ULF) on D1; D4; D7; D1 + D4 + *** group remained *** Exp.1,ULF was collected on D7.5 from all animals and used for quantification of total protein concentration and abundance of *** Exp.2,three in vitro-produced embryos were transferred to the uterine horn ipsilateral to the ovary containing the CL on D7.5 and pregnancy was checked on D25 by ***: In Exp.1,ULF collection on D4 or D7 increased(1.5-to 2.2-folds) the total protein concentration and albumin *** collection on D1 did not alter(P > 0.10) these *** Exp.2,ULF collected on D4 or D7 decreased pregnancy rates to approximately half of that measured in the remaining ***: Subtle perturbations imposed to the native intrauterine milieu,such as those caused by a single,low-volume collection of ULF,profoundly disturbs intrauterine composition and pregnancy *** least 4 d were necessary for the uterus to recover its composition and the functional capacity to carry post-implantation gestation.

关键词： Albumin Embryo Histotroph Protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Impact of hormonal modulation at proestrus on ovarian responses and uterine gene expression of suckled anestrous beef cows

引用

Journal of Animal Science and Biotechnology 2018年第1期9卷 77-90页

作者： Manoel Francisco de Sá Filho Angela Maria Gonella-Diaza Mariana Sponchiado Marcio Ferreira Mendanha Guilherme Pugliesi Roney dos Santos Ramos Sónia Cristina da Silva Andrade Gustavo Gasparin Luiz Lehmann Coutinho Marcelo Demarchi Goissis Fernando Silveira Mesquita Pietro Sampaio Baruselli mario binelli Departamento de Reproducao Animal FMVZ USP Sao Paulo SP Brazil Departamento de Genetica e Biologia Evolutiva IB-USP- Sao Paulo SP Brazil Laboratorio de Biotecnologia Animal ESALQ USP Av Padua Dias Piracicaba SP 11 Brazil Universidade Federal do Pampa Uruguaiana RS Brazil Universidade de Sao Paulo Faculdade de Medicina Veterinriae Zootecnia Departamento de Reprodcao Animal Avenida Duque de Caxias Norte 225 Pirassununga SP Zip Code 13635900 Brazil

Background： This study evaluated the impact of hormonal modulation at the onset of proestrus on ovarian response and uterine gene expression of beef ***： A total of 172 anestrous beef cows were assigned to one of four groups according to the treatment with estradiol cypionate（ECP） and/or equine chorionic gonadotropin（eCG） [CON（n = 43）, ECP（n = 43）, eCG（n = 44）and ECP ＋ eCG（n = 42）].Results： ECP-treated cows（ECP and ECP ＋ eCG groups） presented greater occurrence of estrus（44.6% vs. 65.4%; P = 0.01） and pregnancy per AI [47.1% vs. 33.3%; P = 0.07], but similar progesterone（P4） concentration at subsequent diestrus than cows not treated with ECP（CON and eCG groups）. Nonetheless, eCG-treated cows（eCG and ECP ＋ eCG groups） presented larger follicle at timed AI（12.6 ± 0.3 vs. 13.5 ± 0.3 mm; P = 0.03）, greater ovulation rate（96.5%vs. 82.6%; P = 0.008） and greater P4 concentration at d 6（3.9 ± 0.2 vs. 4.8 ± 0.2 ng/mL; P = 0.001） than cows not treated with eCG（CON and ECP groups）. Next, cows with a new corpus luteum 6 d after TAI were submitted to uterine biopsy procedure. Uterine fragments [CON（n = 6）, ECP（n = 6）] were analyzed by RNA-Seq and a total of 135 transcripts were differentially expressed between groups（73 genes up-regulated by ECP treatment）. Subsequently,uterine samples were analyzed by q PCR（genes associated with cell proliferation）. ECP treatment induced greater abundance of PTCH2（P = 0.07） and COL4 A1（P = 0.02）, whereas suppressed EGFR（P = 0.09） expression. Conversely, eCG treatment increased abundance of HB-EGF（P = 0.06）, ESR2（P = 0.09）, and ITGB3（P = 0.05）, whereas it reduced transcription of ESR1（P = 0.05）. Collectively, supplementation with ECP or eCG at the onset of proestrous of anestrous beef cows influenced ovarian responses, global and specific endometrial gene ***： Proestrus estradiol regulate the endometrial transcriptome, particularly stimulating proliferative activity in the endometrium.

关键词： Cattle eCG Endometrium Estradiol Transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reassessing the AMPK-MTORC1 balance in autophagy in the central nervous system

引用

Neural Regeneration Research 2025年第11期20卷 3209-3210页

作者： Marta García-Juan mario Villa Irene Benito-Cuesta Lara Ordónez-Gutiérrez Francisco Wandosell Centro de Biología Molecular“Severo Ochoa”(CSIC-UAM) Nicolas Cabrera 1Universidad Autónoma de MadridMadridSpain Fisiología Animal Departamento de BiologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Department of Clinical Neuroscience CMM Karolinska Universitetssjukhuset SolnaStockholmSweden Departamento de Bioquímica y Biología Molecular Universidad Complutense de MadridMadridSpain Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas(CIBERNED) MadridSpain

Autophagy is a cellular degradation and recycling system,indispensable for cellular and organ development,homeostasis,and *** cellular process is evolutionarily highly conserved to quality control of many proteins and dysfunctional organelles,which finally recycle components as amino *** process is effective during normal physiology as part of anabolism and plays an additional important role during starvation(Dikic and Elazar,2018).Different types of autophagy have been chara cterized based on their dynamic,mechanism of action,target substrates,and protein *** of them are macroautophagy(hereafter called"autophagy"),microa utophagy,and chape rone-media ted autophagy(Fleming et al.,2022).

关键词： homeostasis system hereafter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A digital earth platform for sustainability

引用

International Journal of Digital Earth 2017年第4期10卷 342-355页

作者： mario Hernandez Engagement Committee Future EarthMexico CityMexico International Society for Digital Earth Mexico CityMexico

Based on the experience of the International Society for Digital Earth(ISDE),this paper describes some challenges foreseen in order to develop a Digital Earth platform that can support the implementation of the Sustainable Development *** use of ready-to-use derived geospatial information is *** Earth’s methodology of‘co-design’aims to bring together natural,social scientists and decision makers to plan and carry out research for *** implies transdisciplinary research,but in order for scientists of different disciplines to work together,they will need to be able to share,access and use common *** is by far not simple!While the good will to share data might exist,the associated technological,ethical and privacy issues are difficult to *** adequate e-infrastructure will be *** could consider to use the SDGs is the basis to develop the desired Digital Earth *** paper,by no means,covers everything for a Digital Earth platform,it aims to trigger research discussions and to have a good view about a starting point.

关键词： Digital earth platform sustainable development Sustainable Development Goals future earth

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PROJECT REGULARITY： DEVELOPMENT AND EVALUATION OF A NEW PROJECT CHARACTERISTIC

引用

Journal of Systems Science and Systems Engineering 2017年第1期26卷 100-120页

作者： Jordy Batselier mario Vanhoucke Faculty of Economics and Business Administration Ghent Universit)/ Tweekerkenstraat 2 9000 Ghent Belgium Technology and Operations Management Area Vlerick Business School Reep 1 9000 Ghent Belgium UCL School of Management University College London Gower Street London WC1E 6BT United Kingdom

The ability to accurately characterize projects novel project characteristic is developed characteristic, called project regularity, is essential to good project management. Therefore, a reflects the value accrue within a project. This expressed in terms of the newly introduced regular/irregular-indicator RI. The widely accepted management system of earned value management （EVM） forms the basis for evaluation of the new characteristic. More concretely, the influence of project regularity on EVMforecasting accuracy is assessed, and is shown to be significant for both time and cost forecasting. Moreover, this effect appears to be stronger than that of the widely used characteristic of project seriality expressed by the serial/parallel-indicator SP. Therefore, project regularity could also be useful as an input parameter for project network generators. Furthermore, the introduction of project regularity can provide project managers with a more accurate indication of the time and cost forecasting accuracy that is to be expected for a certain project and, correspondingly, of how a project should be built up in order to obtain more reliable forecasts during project control.

关键词： Project management earned value management time and cost forecasting empirical database Monte Carlo simulation project control system

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Renewable Energy Penetration Strengthened Using a Reversible Solid Oxide Cell Installed in a Building

引用

CSEE Journal of Power and Energy Systems 2022年第2期8卷 360-368页

作者： mario Lamagna Davide Astiaso Garcia Astronautical Electrical and Energy Engineering Department(DIAEE)Sapienza University of RomeItaly Planning DesignTechnology of Architecture Department(DPDTA)Sapienza University of RomeItaly

The renewable energy source(RES)penetration in end use must be strengthened to reach the prefixed decarbonization targets.A penetration obstacle is represented by the Power Grid,designed with an architecture disinclined to RES ***,different solutions are available to integrate these latter issues without affecting the Grid,among these,the reversible Solid Oxide Cell(rSOC)promises high efficiencies and the possibility to control energy fluxes in both production and *** this study,a series of hourly simulations based on real data were designed to evaluate the rSOC capacity to integrate a large number of RESs in the end use of three different buildings,through analyzing the possible congestion on the Power *** a rSOC model we chose the Smart Energy Hub proposed by Sylfen while for the buildings we selected a school,a hotel,and an office located in Procida,*** results show the rSOC capacity to integrate RES increased from 40%to 62%according to the storage capacity and the building’s hourly load curve and seasonal consumptions.

关键词： Green hydrogen RES integration rSOC power-to-power

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomaterials and additive manufacturing:osteochondral scaffold innovation applied to osteoarthritis(BAMOS project)

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2018年第4期19卷 329-330页

作者： mario MONZóN Departamento de Ingeniería Mecánica Universidad de Las Palmas de Gran CanariaEdificio de Fabricación Integrada

Osteoarthritis(OA)is a degenerative joint disease,typified by a loss of quality of cartilage and changes in bone at the interface of a joint,resulting in pain,stiffness,and reduced mobility."According to the World Hea... 详细信息

关键词：骨关节炎添加剂 ma 革新支架工程活动性疾病

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular reprogramming and inherited peripheral neuropathies: perspectives and challenges

引用

Neural Regeneration Research 2015年第6期10卷 894-896页

作者： mario A.Saporta Department of Neurology Universidade Federal Fluminense

Inherited peripheral neuropathies （or Charcot-Marie-Tooth disease, CMT） are a phenotypically and genetically heterogeneous group of disorders, which are currently untreatable. They are the most common inherited neuromuscular disorder, affecting around 1 in every 2,500 people （over 120,000 people in the US）. Based on clinical neurophysiological and histopathological features, inherited neuropathies can be divided into two major forms： demyelinating （type 1） and axonal （type 2） CMT （Saporta, 2014）.

关键词： gene CMT Cellular reprogramming and inherited peripheral neuropathies perspectives and challenges LITAF

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：