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Foraging, feeding, and physiological stress responses of wild wood mice to increased illumination and common genet cues

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Current Zoology 2018年第4期64卷 409-417页

作者： Alvaro NAVARRO-CASTILLA Isabel BARJA mario DíAZ Department of Biology Faculty of Sciences Universidad Autónoma de Madrid C/Darwin 2 Campus Universitario de Cantolanco Madrid 28049 Spain Department of Biogeography and Global Change Museo Nacional de Ciencias Naturales CSIC Madrid 28006 Spain

In nature, animals are exposed to a broad range of threats imposed by predators, which may strongly influence the ecology of prey species directly or indirectly by affecting their behavior via fear of predation. Here, we studied wood mice Apodemus sylvaticus behavioral and physiological responses to simulated predation risk. Risk avoidance was analyzed by live trapping with control traps and traps treated with feces of common genet Genetta genetta （direct cue of risk） under new moon nights and following by simulated full moon conditions （indirect cue）. The time devoted to foraging behavior and capture time were analyzed by video recording mice activity around traps. Food intake was calculated based on the amount of bait remaining in each trap. Fecal cortico- sterone metabolites （FCMs） were measured by enzyme-immunoassay as indicators of physio- logical stress responses. Fewer wood mice were captured during full moon, yet only non-breeding adult males clearly avoided common genet odor. Mice were captured sooner at night during the simulated full moon conditions and later in predator-treated traps. Foraging activity was lower when individuals faced predator＇s feces, but neither food intake nor FCM levels were affected by predation risk cues. Direct and indirect cues of predation risk selectively affected wood mice behavior, although behavioral responses seem to be modulated by different costs-benefit balances related to the individual＇s perception of risk. The lack of physiological responses to predation risk cues suggests that wood mice did not perceive them as reliable stressors or the response was too small or transient to be measured by FCM.

关键词： common genet fecal predator cues feeding foraging moonlight predator avoidance

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Evaluation of different machine learning methods for land cover mapping of a Mediterranean area using multi-seasonal Landsat images and Digital Terrain Models

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International Journal of Digital Earth 2014年第6期7卷 492-509页

作者： Victor F.Rodriguez-Galiano mario Chica-Rivas Departamento de Geodinamica Universidad de GranadaGranadaSpain Departamento de Analisis Matematico Universidad de GranadaGranadaSpain

Land cover monitoring using digital Earth data requires robust classification methods that allow the accurate mapping of complex land cover *** paper discusses the crucial issues related to the application of different up-to-date machine learning classifiers:classification trees(CT),artificial neural networks(ANN),support vector machines(SVM)and random forest(RF).The analysis of the statistical significance of the differences between the performance of these algorithms,as well as sensitivity to data set size reduction and noise were also ***-5 Thematic Mapper data captured in European spring and summer were used with auxiliary variables derived from a digital terrain model to classify 14 different land cover categories in south ***,statistically similar accuracies of over 91%were obtained for ANN,SVM and ***,the findings of this study show differences in the accuracy of the classifiers,being RF the most accurate classifier with a very simple ***,followed by RF,was the most robust classifier to noise and data *** differences in their performances were only reached for thresholds of noise and data reduction greater than 20%(noise,SVM)and 25%(noise,RF),and 80%(reduction,SVM)and 50%(reduction,RF),respectively.

关键词： land cover remote sensing classification trees random forest neural networks support vector machines

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Detailed electro-dynamic model of an ultra-fast disconnector including the failure mode

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High Voltage 2020年第5期5卷 549-555页

作者： mario Zaja Ali Asghar Razi-Kazemi Dragan Jovcic School of Engineering University of AberdeenAB243UEAberdeenUK

Ultra-fast disconnector(UFD)is a key component of hybrid DC circuit breakers and it is also studied as the main switch in some DC grid topologies.A UFD model suitable for DC grid studies and considering both normal operation and failure mode is *** dynamic motion of contacts is analysed in detail and it is concluded that Thomson coil inductances including parasitic parameters play an important role and it is recommended to use finite element *** arcing mode of UFD is repressed using a variable resistance in series with an ideal *** variable resistance is calculated analytically based on the instantaneous position of contacts and the circuit *** different arc models are recommended:for the air-insulated UFD and SF6 UFD,and in each case,two operating regimes should be considered:high and low *** UFD model is verified for both normal operation and failure mode using measurements on a 5 kV laboratory UFD and the results show very good *** 320 kV SF6 UFD model is evaluated using limited reported results from manufacturers.

关键词： mode dynamic Thomson

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Gastrointestinal lesions associated with spondyloarthropathies

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World Journal of Gastroenterology 2009年第20期15卷 2443-2448页

作者： Ambrogio Orlando Sara Renna Giovanni Perricone mario Cottone Department of General Medicine Pneumology and Nutrition Clinic "V. Cervello" Hospital Palermo University Department of General Medicine Pneumology and Nutrition Clinic"V. Cervello" Hospital Palermo University

Subclinical gut inflammation has been described in up to twothirds of patients with spondyloarthropathies(SpA).Arthritis represents an extraintestinal manifestation of several gastrointestinal diseases, including inflammatory bowel disease(IBD), Whipple's disease, Behcet's disease, celiac disease, intestinal bypass surgery, parasitic infections of the gut and pseudomembranous *** about two thirds of nonsteroidal antiinflammatory drug users demonstrate intestinal *** may manifest as a peripheral or axial *** spondyloarthropathy family consists of the following entities:ankylosing spondylitis, undifferentiated spondyloarthritis, reactive arthritis, psoriatic arthritis, spondyloarthritis associated with IBD, juvenile onset *** topic reviews the major gastrointestinal manifestations that can occur in patients with SpA and in nonsteroidal anti inflammatory drugs users.

关键词： Gastrointestinal diseases Inflammation Inflammatory bowel diseases Crohn's disease Arthritis Spondylosis Ankylosing spondylitis Nonsteroidal anti- inflammatory drugs

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Major regulatory mechanisms involved in sperm motility

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Asian Journal of Andrology 2017年第1期19卷 5-14页

作者： Rute Pereira Rosalia Sa Alberto Barros mario Sousa Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar University of Porto (ICBAS-UP) Rua Jorge Viterbo Ferreira 2284050-313 Porto Portugal and Multidisciplinary Unit for Biomedical Research-UMIB ICBAS-UP Centre for Reproductive Genetics Alberto Barros Av. do Bessa 2401° Dto. Frente 4100-012 Porto Portugal Department of Genetics Faculty of Medicine University of Porto. Alameda Prof. Hernani Monteiro 4200-319 Porto Portugal and Institute of Health Research an Innovation (13S) University of Porto Portugal.

The genetic bases and molecular mechanisms involved in the assembly and function of the flagellum components as well as in the regulation of the flagellar movement are not fully understood, especially in humans. There are several causes for sperm immotility, of which some can be avoided and corrected, whereas other are related to genetic defects and deserve full investigation to give a diagnosis to patients. This review was performed after an extensive literature search on the online databases PubMed, ScienceDirect, and Web of Science. Here, we review the involvement of regulatory pathways responsible for sperm motility, indicating possible causes for sperm immotility. These included the calcium pathway, the cAMP-dependent protein kinase pathway, the importance of kinases and phosphatases, the function of reactive oxygen species, and how the regulation of cell volume and osmolarity are also fundamental components. We then discuss main gene defects associated with specific morphological abnormalities. Finally, we slightly discuss some preventive and treatments approaches to avoid development of conditions that are associated with unspecified sperm immotility. We believe that in the near future, with the development of more powerful techniques, the genetic causes of sperm immotility and the regulatory mechanisms of sperm motility will be better understand, thus enabling to perform a full diagnosis and uncover new therapies.

关键词： antioxidants calcium membrane channels protein kinases sperm genetic abnormalities sperm motility

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A 3R-Tau-mediated mechanism in oligodendrocytes:could it be the key for neuroprotection after stroke?

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Neural Regeneration Research 2021年第12期16卷 2401-2402页

作者： mario Villa Gonzalez Maria JoséPérez-Álvarez Departamento de Biología(Fisiología Animal) Facultad de CienciasUniversidad Autónoma de MadridMadridSpain Centro de Biología Molecular“Severo Ochoa” Departamento de Neuropatología Molecular CSIC-UAMMadridSpain Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas(CIBERNED) MadridSpain

Anoverview of cerebral ischemia:Cerebrovascular accident or stroke have a high global *** most common types of stroke are ischemic,accounting for 87%of the total number,and they are triggered by a reduction or interruption of blood flow to the central nervous system,usually caused by a thrombus,embolus or atherosclerotic *** severity of brain damage caused by this kind of stroke is directly related to the size of the vessel that is occluded and the duration of occlusion.

关键词： cerebral ischemia damage

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Non-coding RNAs and other determinants of neuroinflammation and endothelial dysfunction:regulation of gene expression in the acute phase of ischemic stroke and possible therapeutic applications

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Neural Regeneration Research 2021年第11期16卷 2154-2158页

作者： mario Daidone Marco Cataldi Antonio Pinto Antonino Tuttolomondo Department of Health Promotion Maternal and Infant CareInternal Medicine and Medical Specialties“G.D’Alessandro”University of PalermoPiazza delle Cliniche n.2PalermoItaly

Ischemic stroke occurs under a variety of clinical conditions and has different pathogeneses,resulting in necrosis of brain *** pathogenesis is characterized by neuroinflammation and endothelial *** of the main processes triggered in the early stages of ischemic damage are the rapid activation of resident inflammatory cells(microglia,astrocytes and endothelial cells),inflammatory cytokines,and translocation of intercellular nuclear *** in stroke includes all the processes mentioned above,and it consists of either protective or detrimental effects concerning the“polarization”of these *** polarization comes out from the interaction of all the molecular pathways that regulate genome expression:the epigenetic *** recent years,new regulation mechanisms have been cleared,and these include non-coding RNAs,adenosine receptors,and the activity of mesenchymal stem/stromal cells and *** reviewed how long non-coding RNA and microRNA have emerged as an essential mediator of some neurological *** also clarified that their roles in cerebral ischemic injury may provide novel targets for the treatment of ischemic *** date,we do not have adequate tools to control pathophysiological processes associated with *** goal is to review the role of non-coding RNAs and innate immune cells(such as microglia and mesenchymal stem/stromal cells)and the possible therapeutic effects of their modulation in patients with acute ischemic stroke.A better understanding of the mechanisms that influence the“polarization”of the inflammatory response after the acute event seems to be the way to change the natural history of the disease.

关键词： acute phase cerebrovascular disease endothelial dysfunction epigenetics genetics neuroiflammation non-coding RNAs stroke

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Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

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Journal of Environmental Sciences 2015年第10期27卷 102-111页

作者： Jaqueline Bianchi mario Sérgio Mantovani Maria Aparecida Marin-Morales Department of Biology Institute of BiosciencesSao Paulo State University(UNESP) Department of General Biology Biological Science CentreUniv Estadual de Londrina

Based on the concentration of Malathion used in the field, we evaluated the genotoxic potential of low concentrations of this insecticide on meristematic and F1 cells of Allium cepa and on rat hepatoma tissue culture（HTC cells）. In the A. cepa, chromosomal aberrations（CAs）, micronuclei（MN）, and mitotic index（MI） were evaluated by exposing the cells at 1.5,0.75, 0.37, and 0.18 mg/m L of Malathion for 24 and 48 hr of exposure and 48 hr of recovery time. The results showed that all concentrations were genotoxic to A. cepa cells. However,the analysis of the MI has showed non-relevant effects. Chromosomal bridges were the CA more frequently induced, indicating the clastogenic action of Malathion. After the recovery period, the higher concentrations continued to induce genotoxic effects, unlike the observed for the lowest concentrations tested. In HTC cells, the genotoxicity of Malathion was evaluated by the MN test and the comet assay by exposing the cells at 0.09, 0.009, and0.0009 mg/5 m L culture medium, for 24 hr of exposure. In the comet assay, all the concentrations induced genotoxicity in the HTC cells. In the MN test, no significant induction of MN was observed. The genotoxicity induced by the low concentrations of Malathion presented in this work highlights the importance of studying the effects of low concentrations of this pesticide and demonstrates the efficiency of these two test systems for the detection of genetic damage promoted by Malathion.

关键词： HTC Chromosomal aberration Micronuclei Comet assay Pesticide

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TOWARDS AVOIDING THE HIDDEN TRAPS IN QFD DURING REQUIREMENTS ESTABLISHMENT

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Journal of Systems Science and Systems Engineering 2015年第3期24卷 316-336页

作者： Xinwei Zhang Shurong Tong Hakki Eres Keqin Wang mario Kossmann School of Management Northwestern Polytechnical University Engineering and the Environment University of Southampton Airbus UK AirbusBristol BS99 7ARUK

The Quality Function Deployment （QFD） is a useful and representative methodology to transform customer needs into different level of requirements in a system hierarchy. Simplifications that are based on assumptions are ubiquitous in the QFD, but these underlying assumptions possibly do not hold true, which renders the simplifications unjustified. Additionally, these assumptions are usually not verified within the context of the application domain. This paper identifies and illustrates eight hidden traps in QFD during the process of establishing the requirements, where the assumptions, and therefore, the simplifications made are not reasonable. These traps are implicit in the understanding of customer needs, establishment of system requirements and the flow down of these requirements to lower levels of the system hierarchy. Suggestions are given to help avoiding these hidden traps, thereby eliminating or alleviating their potentially detrimental effects. The intent of the paper is to make readers aware of these traps when applying QFD for the establishment of requirements, so that they may utilize QFD with a better understanding of its limitations and develop higher quality specifications.

关键词： QFD requirements establishment value decision-making

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Estimation of Low Nutrients in Tomato Crops Through the Analysis of Leaf Images Using Machine Learning

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Journal of Artificial Intelligence and Technology 2021年第2期1卷 131-137页

作者： Hiram ponce Claudio Cevallos Ricardo Espinosa Sebastián Gutiérrez Universidad Panamericana Facultad de IngenieríaAugusto Rodin 498Ciudad de México 03920México Universidad Panamericana Facultad de IngenieríaJosemaría Escriváde Balaguer 101Aguascalientes 20290México

Tomato crops are considered the most important agricultural products ***,the quality of tomatoes depends mainly on the nutrient *** inspection is made by farmers to anticipate the nutrient deficiency of the ***,precision agriculture has explored opportunities to automate nutrient level *** work has demonstrated that a convolutional neural network is able to estimate low nutrients in tomato plants using images of their ***,the performance of the convolutional neural network was not ***,this work proposes a novel convolutional neural network-based classifier,namely,CNN+AHN,for estimating low nutrients in tomato crops using an image of the tomato *** CNN+AHN incorporates a set of convolutional layers as the feature extraction part,and a supervised learning method called artificial hydrocarbon network as the dense *** combinations of the architecture of CNN+AHN were *** results showed that our best CNN+AHN classifier is able to estimate low nutrients in tomato plants with an accuracy of 95.57%and F1-score of 95.75%,outperforming the literature.

关键词： agriculture image processing deep learning computer vision color analysis

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