检索结果-南通市图书馆

Ocular flora in patients undergoing intravitreal injections: antibiotic resistance patterns and susceptibility to antiseptic picloxydine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2020年第1期13卷 85-92页

作者： Maria V.Budzinskaya Anait S.Khalatyan marina G.Strakhovskaya Vladimir G.Zhukhovitsky Scientific Research Institute of Eye Diseases Moscow 119021Russia Department of Biology Lomonosov Moscow State UniversityMoscow 119234Russia Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies Federal Medical and Biological Agency of RussiaMoscow 115682Russia Gamaleya national Research Centre for Epidemiology and Microbiology Moscow 123098Russia

AIM: To study antibiotic resistance patterns and susceptibility to eye antiseptic picloxydine of conjunctival flora in patients undergoing intravitreal injections(IVIs).METHODS: Conjunctival swabs were taken in 4 groups of patients, 20 patients in each group(n=80): without IVIs and ophthalmic operations in history(group n1;control group);with the first IVI and antibiotic eye drops Tobrex applied 3 d before IVI and 5 d after it(group n2);with 20 or more IVIs and repeated courses of antibiotic eye drops(group n3);with the first IVI and antiseptic eye drops Vitabact(picloxydine) applied 3 d before IVI and 5 d after it(group n4). In groups n2 and n4 swabs were taken at baseline and after the treatment. Efficacy of picloxydine in inhibition of growth of conjunctival isolates susceptible and resistant to antibiotic was studied in vitro. Minimal inhibition concentrations(MIC) were determined with microdilution ***: Two of the three patients who had to undergo the IVI procedure showed conjunctiva bacterial contamination. Along with few Staphylococcus aureus and Gram-negative isolates susceptible to most antibiotics, the majority(71%-77%) of causative agents were coagulase-negative Staphylococci(Co nS), 40%-50% of which were multidrug resistant(MDR). Eye disinfection in the operating room and peri-injection courses of Tobrex or Vitabact resulted in total elimination of isolates found at baseline. However, in 10% and 20% of patients, respectively, recolonization of the conjunctiva with differing strains occurred. In patients with repeated IVI and Tobrex/Maxitrol treatment, the conjunctival flora showed high resistance rates: 90% of Co nS were MDR. In the in vitro study, picloxydine showed bactericidal effect against Staphylococci isolates both antibiotic resistant and susceptible with MIC≥13.56 μg/m L. Incubation of bacteria for 15 min in Vitabact eye drops, commercially available form of picloxydine, 434 μg/m L, showed total loss of colony forming units of all teste

关键词： intravitreal injections conjunctival isolates antibiotic resistance picloxydine Vitabact

A pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2023年第3期37卷 213-224页

作者： Gelena Kakurina marina Stakheeva Elena Sereda Evgenia Sidenko Olga Cheremisina Evgeny Choinzonov Irina Kondakova Cancer Research Institute Tomsk National Research Medical CenterRussian Academy of SciencesTomsk 634009Russia Department of Biochemistry and Molecular Biology Faculty of Medicine and BiologySiberian State Medical UniversityTomsk 634050Russia.

Circulating tumor cells(CTCs)play an important role in tumor metastases,which is positively correlated with an increased risk of ***-binding proteins,including cofilin(CFL1),profilin 1(PFn1),and adenylate cyclase-associated protein 1(CAP1),are thought to be involved in tumor cell motility and metastasis,specifically in head and neck squamous cell carcinoma(HnSCC).However,currently,there are no published studies on CFL1,PFn1,and CAP1 in CTCs and leukocytes in HnSCC *** assessed serum levels of CFL1,PFn1,and CAP1 and the number of CTCs and leukocytes containing these proteins in blood from 31 HnSCC patients(T1-4n0-2M0).The analysis used flow cytometry and an enzyme-linked immunosorbent assay *** found that CAP1+CTCs and CAP1+leukocyte subpopulations were prevalent in these HnSCC patient samples,while the prevalence rates of CFL1+and PFn1+CTCs were relatively *** with stage T2-4n1-2M0 had CFL1+and PFn1+CTCs with an elevated PFn1 serum level,compared with the T1-3n0M0 *** summary,the PFn1 serum level and the relative number of PFn1+CD326+CTCs could be valuable prognostic markers for HnSCC *** current study is the first to obtain data regarding the contents of actin-binding proteins(ABPs)in CTCs,and leukocytes in blood from HnSCC *** is also the first to assess the relationship between the number of CTCs subgroups and disease characteristics.

关键词： head and neck squamous cell carcinoma metastasis circulating tumor cells actin-binding proteins adenylyl cyclase-associated protein 1

Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Translational neurodegeneration 2023年第1期12卷 722-744页

作者： Cristina de Dios Xenia Abadin Vicente Roca-Agujetas marina Jimenez-Martinez Albert Morales Ramon Trullas Montserrat Mari Anna Colell Department of Cell Death and Proliferation Institut d’Investigacions Biomèdiques de BarcelonaConsejo Superior de Investigaciones Científicas(CSIC)Institut d’Investigacions Biomèdiques August Pi I Sunyer(IDIBAPS)BarcelonaSpain Centro de Investigación Biomédica en Red Sobre Enferme-dades neurodegenerativas(CIBERnED) MadridSpain Department of Biomedicine Faculty of MedicineUniversitat de BarcelonaBarcelonaSpain Present Address:Department of Biochemistry and Molecular Biology Faculty of PharmacyUniversidad de Sevilla.Instituto de Biomedicina de Sevilla(IBiS)-Hospital Universitario Virgen del Rocío/CSICSevilleSpain Present Address:Department of Clinical Immunology and Rheumatology Amsterdam UMCAmsterdamNetherlands

Background Persistent inflammatory response in the brain can lead to tissue damage and *** Alzheimer’s disease(AD),there is an aberrant activation of inflammasomes,molecular platforms that drive inflammation through caspase-1-mediated proteolytic cleavage of proinflammatory cytokines and gasdermin D(GSDMD),the executor of ***,the mechanisms underlying the sustained activation of inflammasomes in AD are largely *** have previously shown that high brain cholesterol levels promote amyloid-β(Aβ)accumulation and oxidative ***,we investigate whether these cholesterol-mediated changes may regulate the inflam-masome *** SIM-A9 microglia and SH-SY5Y neuroblastoma cells were cholesterol-enriched using a water-soluble cholesterol *** exposure to lipopolysaccharide(LPS)plus muramyl dipeptide or Aβ,activation of the inflammasome pathway was analyzed by immunofluorescence,ELISA and immunoblotting ***-labeled Aβwas employed to monitor changes in microglia *** medium was used to study how microglia-neuron interrelationship modulates the inflammasome-mediated *** In activated microglia,cholesterol enrichment promoted the release of encapsulated IL-1βaccompanied by a switch to a more neuroprotective phenotype,with increased phagocytic capacity and release of neurotrophic *** contrast,in SH-SY5Y cells,high cholesterol levels stimulated inflammasome assembly triggered by both bacterial toxins and Aβpeptides,resulting in GSDMD-mediated ***(GSH)ethyl ester treatment,which recovered the cholesterol-mediated depletion of mitochondrial GSH levels,significantly reduced the Aβ-induced oxidative stress in the neuronal cells,resulting in lower inflammasome activation and cell ***,using conditioned media,we showed that neuronal pyroptosis affects the function of the cholesterol-enriched microglia,lowering its phagocytic activity and,the

关键词： neuroinflammation Mitochondrial oxidative stress Phagocytosis Alzheimer’s disease DAM signature nLRP3

Association of vitamin A status with lung function in children and adolescents with cystic fibrosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pediatric Investigation 2021年第2期5卷 125-129页

作者： Ioanna Loukou Maria Moustaki Olympia Sardeli marina Plyta Christina n.Katsagoni Konstantinos Douros Cystic Fibrosis Department “Agia Sofia”Children’s HospitalAthensGreece Pediatric Allergy and Respiratory Unit Third Department of Pediatrics“Attikon”University HospitalNational and Kapodistrian University of AthensSchool of MedicineAthensGreece

Importance Vitamin A(or retinol)has considerable antioxidative and anti-inflammatory attributes and it may have protective effects on the respiratory health of patients with cystic fibrosis(CF).This issue,however,remains *** The purpose of the present study was to investigate the relationship between serum retinol levels(SRL)and force expiratory volume in 1 second(FEV1)in patients with *** All patients with pancreatic insufficiency attending the CF Department of“Agia Sofia”Children’s Hospital in Athens,Greece,aged 6 to 19 years during the 2012–2016 period,who could perform spirometry effectively,were included in the *** impact of SRL on FEV1 was examined longitudinally and analyzed with generalized estimating *** The study included 231 patients and 851 SRL measurements were *** 25(3.2%)cases the SRL were below the 5th percentile of reference distribution;none was above the 95th *** analysis showed that SRL was positively correlated with the FEV1(Pnterpretation In this sample of children and adolescents with CF,vitamin A deficiency was *** results suggest a positive relationship between SRL and FEV1.

关键词： Cystic fibrosis Vitamin A Retinol Lung function

Role of IL-10 and TGF-β1 in local immunosuppression in HPV-associated cervical neoplasia

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Oncology 2014年第4期5卷 753-763页

作者： Kirvis Torres-Poveda Margarita Bahena-Román Claudia Madrid-González Ana I Burguete-García Víctor Hugo Bermúdez-Morales Oscar Peralta-Zaragoza Vicente Madrid-marina Chronic Infection and Cancer Division Research Center on Infectious Diseases Instituto Nacional de Salud Pública 62100 Cuernavaca Morelos Mexico

Cervical cancer is a worldwide disease that constitutes a significant public health problem, especially in developing countries, not only due to its high incidence but also because the most affected population comprises women who belong to marginalized socio-economic classes. Clinical and molecular research has identified immunological impairment in squamous intraepithelial cervical lesions and cervical cancer patients. Human Papillomavirus(HPV) has several mechanisms for avoiding the immune system: it down-regulates the expression of interferon and upregulates interleukin(IL)-10and transforming growth factor(TGF)-β1 to produce a local immunosuppressive environment, which, along with altered tumor surface antigens, forms an immunosuppressive network that inhibits the antitumor immune response. In this review we analyzed the available data on several deregulated cellular immune functions in patients with nIC Ⅰ, nIC Ⅱ and nIC Ⅲ and cervical cancer. The effects of immunosuppressive cytokines on innate immune response, T-cell activation and cellular factors that promote tumor cell proliferation in cervical cancer patients are summarized. We discuss the functional consequences of HPV E2, E6, and E7 protein interactions with IL-10 and TGF-β1 promoters in the induction of these cytokines and postulate its effect on the cellular immune response in squamous intraepithelial cervical lesions and cervical cancer patients. This review provides a comprehensive picture of the immunological functions of IL-10 and TGF-β1 in response to HPV in humans.

关键词： Cervical cancer Immunosuppression Interleukin-10 Transforming growth factor-β1 Human Papillomavirus

Ecological intensification of cropping systems enhances soil functions,mitigates soil erosion,and promotes crop resilience to dry spells in the Brazilian Cerrado

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Soil and Water Conservation Research 2021年第4期9卷 591-604页

作者： Lucas de Castro Moreira da Silva Junior Cesar Avanzi Devison Souza Peixoto marina neves Merlo Emerson Borghi Alvaro Vilela de Resende Salvador Francisco Acuna-Guzman Bruno Montoani Silva Department of Soil Science Federal University of Lavras(UFLA)37200-900LavrasMGBrazil Department of Water Resources Federal University of Lavras(UFLA)37200-900LavrasMGBrazil Embrapa Maize and Sorghum Brazilian Agricultural Research Corporation(EMBRAPA)PO Box:28535701-970Sete LagoasMGBrazil Department of Physics Federal University of Lavras(UFLA)37200-900LavrasMGBrazil Center for Engineering and Industrial Development(CIDESI) Pie de la Cuesta 702Desarrollo San PabloQuerétaroQro.76125Mexico

Water scarcity threatens global food security and agricultural systems are challenged to achieve high yields while optimizing water *** deficit can be accentuated by soil physical degradation,which also triggers water losses through runoff and consequently soil *** soil health in cropping systems within the Brazilian Cerrado biome have been surveyed throughout the years,information about soil erosion impacts and its mitigation are still not well understood;especially concerning the role of cropping system diversification and its effects on crop ***,the aim of this study was to assess whether ecological intensification of cropping systems-inclusion of a consorted perennial grass and crop rotation-could promote soil coverage and consequently decrease water erosion and soil,water,and nutrient *** work studied the effects of crop rotation and consorted Brachiaria,along with different levels of investment in fertilization on soil physical quality and on soil,water,and nutrient losses,and crop *** proved that soybean monoculture(SS)is a system of low sustainability even under no-till in the Brazilian Cerrado *** exhibited high susceptibility to soil,water,and nutrient losses,causing low crop *** results showed that water losses in SS cropping system were approximately 10%of the total annual rainfall,and total K losses would require an additional 35%of K ***,ecological intensification of cropping systems resulted in enhanced soil environmental and agronomic functions,increased grain yield,and promoted soil and water conservation:high soil cover rate,and low soil,water and nutrient *** intensification proved to be an adequate practice to boost crop resilience to water deficit in the Brazilian Cerrado.

关键词： Crop rotation Grain production nutrient losses Soil physical quality Tropical region

Arrhythmogenic cardiomyopathy with left ventricular involvement versus ischemic heart disease: lessons learned from the family study and the reviewed autopsy of a young male

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Forensic Sciences Research 2019年第3期4卷 274-279页

作者： Pilar Molina Jorge Sanz-Sánchez Manuel Fenollosa marina Martínez-Matilla Juan Giner Esther Zorio Servicio de Patología Instituto de Medicina Legal y Ciencias Forenses de ValenciaValenciaEspana Unidad de Valoración del Riesgo de Muerte Súbita Familiar and Unidad de Cardiopatías Familiares Muerte Súbita y Mecanismos de Enfermedad(CaFaMuSMe)Instituto de Investigación Sanitaria La FeValenciaEspana Servicio de Cardiología Hospital Universitario y Politécnico La FeValenciaEspana Unidad Genómica Instituto de Investigación Sanitaria La FeValenciaEspana

Ischemic heart disease (IHD) is the leading cause of sudden cardiac death (SCD) and often non-thrombosed severe coronary stenoses with or without myocardial scars are *** dominant arrhythmogenic cardiomyopathy (LDAC) is a life-threating rare disease which has been more thoroughly studied in the last *** macroscopic study of an SCD victim was conducted and re-evaluated 9years *** cardiological work-up in his firstdegree relatives initially comprised an electrocardiogram (ECG) and an *** they were re-evaluted 9years later,a cardiac magnetic resonance,an ECG-monitoring,an exercise testing and a genetic study were performed and the pedigree was extended *** 2008,an IHD was suspected in the sports-triggered SCD of a 37-year-old man upon the postmortem (75% stenosis of the left main and circumflex coronary arteries;the subepicardial left ventricular fibrofatty infiltration with mild myocardial degeneration was assumed to be a past myocardial infarction).no cardiomyopathy was identified in any of the two proband's *** years thereafter,distant relatives were diagnosed with LDAC due to a pathogenic desmoplakin *** reanalysis of the two sisters showed ventricular arrhythmias in one of them without structural heart involvement and the reviewed postmortem of the proband was reclassified as LDAC based on the fibrofatty infiltration;both were mutation *** completion of the family study on 19 family members yielded one SCD due to LDAC (the proband),three living patients diagnosed with LDAC (two with a defibrillator),one mutation carrier without structural ventricular involvement,and 14 healthy relatives (who were discharged) with a very good co-segregation of the *** rare,LDAC exists and sometimes its differential diagnosis with iHD has to be *** previous postmortem misdiagnoses can help family screening to further prevent SCDs.

关键词： Forensic sciences forensic pathology ischemic heart disease arrhythmogenic cardiomyopathy family study

Analysis of Environmental Bonds in Mexican Pacific Major Ship Grounding

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2013年第8期4卷 65-71页

作者： marina Mondragon Anamaría Escofet Department of Marine Ecology Centro de Investigación Científica y de Educación Superior de Ensenada Ensenada México.

Environmental bonds are a means to accounting for future environmental costs, yet examples of application in real-life scenarios are scarce, and estimation of fairness of the bonds is still poorly explored. We examined a recent Mexican Pacific maritime accident in which the aid of extra environmental assessments addressing long-lasting effects was needed, and contributed to the decision-making process of environmental authorities by means of an arrangement based on the legal figure of the environmental bond. The basis to estimate the bond was the cost of activities needed to fulfill compensatory measures, specifically, the cost of research programs that would set a baseline for monitoring long-lasting damage to the bottom. In order to proceed with analysis of environmental bonds, we employed cluster analysis for comparing mean depth, grain size, and composition and abundance of benthic fauna at the three sites set for monitoring long-lasting effects, under the assumption that no differences would mean bond overestimation whereas differences would mean fairness. The results show that the three sites were different enough to justify the spatial setting of three separate sampling campaigns, and that the bond was reasonably not overestimated. The approach appears suitable to address in a semi-analytical way current inquiries regarding fairness of the environmental bonds and so may contribute to the state of the art.

关键词： Soft Bottom Impacts Environmental Bonds Cluster Analysis Todos Santos Bay

Microenvironment and endocrine resistance in breast cancer:Friend or foe?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Oncology 2015年第6期6卷 207-211页

作者： Sol Recouvreux Rocío Sampayo María Inés Díaz Bessone marina Simian Instituto de Oncología "Angel H. Roffo" Escuela de Humanidades and Instituto de nanosistemas Universidad Nacional de San Martín

Breast cancer affects one in eight women around the world. Seventy five percent of these patients have tumors that are estrogen receptor positive and as a consequence receive endocrine therapy. However,about one third eventually develop resistance and cancer reappears. In the last decade our vision of cancer has evolved to consider it more of a tissuerelated disease than a cell-centered one. This editorial argues that we are only starting to understand the role the tumor microenvironment plays in therapy resistance in breast cancer. The development of new therapeutic strategies that target the microenvironment will come when we clearly understand this extremely complicated scenario. As such,and as a scientific community,we have extremely challenging work ahead. We share our views regarding these matters.

关键词： Breast cancer Tumor microenvironment Endocrine resistance Tamoxifen Stroma Estrogen receptor Aromatase inhibitors Cancer stem cells