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mRNA transcriptome profiling of human hepatocellular carcinoma cells HepG2 treated with Catharanthus roseus-silver nanoparticles

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World Journal of Hepatology 2023年第3期15卷 393-409页

作者： Nur Asna Azhar Siti Aishah Abu Bakar marimuthu citartan Nor Hazwani Ahmad Department of Biomedical Science Advanced Medical and Dental InstituteUniversiti Sains MalaysiaKepala Batas 13200Pulau PinangMalaysia Liver Malignancies Research Program Advanced Medical and Dental InstituteUniversiti Sains MalaysiaKepala Batas 13200Pulau PinangMalaysia Faculty of Bioresources and Food Industry Universiti Sultan Zainal AbidinBesut CampusBesut 22200TerengganuMalaysia

BACKGROUND The demand for the development of cancer nanomedicine has increased due to its great therapeutic value that can overcome the limitations of conventional cancer ***,the presence of various bioactive compounds in crude plant extracts used for the synthesis of silver nanoparticles(AgNPs)makes its precise mechanisms of action *** To assessed the mRNA transcriptome profiling of human HepG2 cells exposed to Catharanthus roseus ***(***)-*** The proliferative activity of hepatocellular carcinoma(HepG2)and normal human liver(THLE3)cells treated with *** were measured using MTT *** RNA samples were extracted and sequenced using BGIseq500 *** is followed by data filtering,mapping,gene expression analysis,differentially expression genes analysis,Gene Ontology analysis,and pathway *** The mean IC 50 values of *** on HepG2 was 4.38±1.59μg/mL while on THLE3 cells was 800±1.55μg/*** profiling revealed an alteration of 296 *** induced the expression of stress-associated genes such as MT,HSP and *** signalling pathways were potentially activated through MAPK,TNF and TGF pathways that are responsible for apoptosis and cell cycle *** alteration of ARF6,EHD2,FGFR3,RhoA,EEA1,VPS28,VPS25,and TSG101 indicated the uptake of ***-AgNPs via both clathrin-dependent and clathrinindependent *** This study provides new insights into gene expression study of biosynthesised AgNPs on cancer *** cytotoxicity effect is mediated by the aberrant gene alteration,and more interestingly the unique selective antiproliferative properties indicate the *** as an ideal anticancer candidate.

关键词： Catharanthus roseus HepG2 Silver nanoparticles Transcriptome oxidative stress Apoptosis Cell cycle

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