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Analysis of malaria diagnosis discrepancies between RDTs and microscopy by nested PCR

Journal of Biomedical Science and Engineering 2013年第10期6卷 967-972页

作者： Denise Patricia Mawili-Mboumba marielle karine bouyou-akotet Christelle Offouga Mbouoronde Maryvonne Kombila Department of Parasitology-Mycology Faculty of Medicine Université des Sciences de la Santé Libreville Gabon

A total of 415 samples from febrile children exhibiting either concordant (n = 108) or discordant (n = 307) results between microscopy, the gold standard diagnostic test, and two Rapid Diagnostic Tests (RDTs), OptiMAL-IT?(pLDH) and Acon?HRP2, were analysed using polymerase chain reaction (PCR) from May to December 2011 in Gabon. The aim of the study was to analyse these discrepancies using poly-merase chain reaction (PCR). Nested PCR targeting the Plasmodium ssrRNA gene was used to distinguish P. falciparum, P. malariae and P. ovale. Plasmodium falciparum was the only malaria species identified. Discrepancies frequently involved samples that were negative by microscopy and positive by Acon?HRP2 (90%) or Optimal-it?(86%). The PCR assay detected submicroscopic infection in almost 23% of the microscopy-negative samples, whereas plasmodial DNA was not found in 77% of the Acon?HRP2 positive-microscopy negative samples. Although results obtained with Optimal-it? were more frequently concordant with those of PCR genotyping, the low specificity of Optimal-iT?for non-falciparum malaria parasite detection resulted in a high proportion of false negative RDTs (90%) and a high frequency of tests with faint line intensity. The present study highlights the specific attributes of the different methods used to identify malaria parasite below the microscopy level of detection. RDT results that were discordant with either microscopy or PCR as the gold standard could represent a challenge for rapid, accurate fever case management in malaria endemic areas. It is necessary to pursue the development of more precise and more sensitive point-of-care diagnostic tools for malaria.

关键词： Plasmodium falciparum RDTs Microscopy PCR Gabon

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Correction to:prevalence of and risk factors for malaria,filariasis,and intestinal parasites as single infections or co-infections in different settlements of Gabon,Central Africa

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Infectious Diseases of Poverty 2018年第1期7卷 373-373页

作者： Noe Patrick M’bondoukwe Eric Kendjo Denise Patricia Mawili-Mboumba Jeanne Vanessa Koumba Lengongo Christelle Offouga Mbouoronde Dieudonné Nkoghe Fousseyni Toure marielle karine bouyou-akotet Department of Parasitology-Mycology Faculty of MedicineUniversity of Health SciencesP.O.Box 4009LibrevilleGabon International Center for Medical Research of Franceville FrancevilleGabon

Correction Unfortunately,the original article[1]contained some *** table title of Tables 4,5,6,7 were interchanged by mistake and displayed incorrectly in the *** correct table titles of Tables 4,5,6,7 can be found *** 4:Distribution of intestinal parasite species,according to the *** 5:Risk factors for *** *** 6:Risk factors for *** 7:Risk factors for polyparasitism(bivariate analysis).

关键词： infections prevalence parasite

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Prevalence of and risk factors for malaria,filariasis,and intestinal parasites as single infections or co-infections in different settlements of Gabon,Central Africa

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Infectious Diseases of Poverty 2018年第1期7卷 60-76页

作者： Noe Patrick M’bondoukwe Eric Kendjo Denise Patricia Mawili-Mboumba Jeanne Vanessa Koumba Lengongo Christelle Offouga Mbouoronde Dieudonne Nkoghe Fousseyni Toure marielle karine bouyou-akotetAuthor details Department of Parasitology-Mycology Faculty of MedicineUniversity of Health SciencesP.O.Box 4009LibrevilleGabon International Center for Medical Research of Franceville FrancevilleGabon.

Background:Malaria,filariasis,and intestinal parasitic infections(IPIs)are common and frequently overlap in developing *** prevalence and predictors of these infections were investigated in three different settlements(rural,semi-urban,and urban)of ***:During cross-sectional surveys performed from September 2013 to June 2014,451 individuals were *** addition,blood and stool samples were analysed for the presence of Plasmodium,filarial roundworm,intestinal protozoan,and helminth ***:Intestinal parasitic infections(61.1%),including intestinal protozoa(56.7%)and soil-transmitted helminths(STHs)(22.2%),predominated,whereas Plasmodium falciparum(18.8%),Loa loa(4.7%),and Mansonella perstans(1.1%)were less *** and STHs were mainly found in rural settlements,whereas a higher plasmodial infection prevalence rate was observed in the periurban *** most common IPI was blastocystosis(48.6%),followed by ascaridiasis(13.7%),trichuriasis(11.8%),amoebiasis(9.3%),giardiasis(4.8%),and strongyloidiasis(3.7%).Hookworm was detected in one adult from rural *** had a higher prevalence of Blastocystis hominis and STHs,whereas Giardia duodenalis was more frequently observed among children aged below 5 years(P<0.01).The polyparasitism rate was 41.5%,with 7.0%Plasmodium-IPIs and 1.8%Plasmodium-STH *** multivariate analysis showed that living in a suburban area,belonging to the age group of 5-15 years,having none or a secondary education,or having an open body water close to home were significant risk factors for malaria(P≤0.01).For STH infections,identified risk factors were drinking untreated water and living in a rural area(P≤0.04).No significant predictors were identified for IPIs and malaria-IPI ***:This study reports a high prevalence of IPIs and intestinal protozoa,but a low rate of malaria-IPI co-infections in the study *** in the living conditions of the popula

关键词： Malaria Filariasis Intestinal parasitic infections Soil-transmitted helminths Co-infection Risk factors Gabon

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