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Journal of Renewable Materials 2019年第1期7卷 73-87页

作者： Ingrid souza Vieira da silva Natália soares Prado Patrícia Gontijo de Melo danilo Campion Arantes Mara Zeni Andrade Harumi Otaguro daniel Pasquini Instituto de Química Universidade Federal de UberlândiaCampus Santa MônicaAv.João Naves deÁvila2121Uberlândia-MG38400-902Brazil Instituto Federal do Triângulo Mineiro Campus Uberaba Rua João Batista Ribeiro4000Uberaba-MG38064-790Brazil departamento de Ciências Naturais(DCNAT) Universidade Federal de São João Del-ReiPraça D.Helvécio74São João Del-Rei-MG36301-160Brazil Universidade de Caxias do Sul Rua Francisco Getúlio Vargas1130Caxias do Sul-RS95070-970Brazil

In this work,nine different types of edible coating based on pectin,cellulose nanocrystals,glycerol,and essential oil of lemongrass were prepared and used to coat strawberries with a film formed directly on the surface of the coated *** effects of the different edible coatings on refrigerated fruits in terms of weight loss,titratable acidity,total soluble solids,pH,and anthocyanin content was evaluated after 2 days,4 days,6 days,and 8 days of *** of the edible coatings reduced the weight loss of the coated strawberries and the anthocyanin *** total soluble solids content of or uncoated fruit increase more markedly than that of coated *** contrast,pH was maintained for both coated and uncoated *** edible coatings were effective in minimizing of the weight loss,without worsening the physical chemistry *** treatments T5 and T9 presented the best results.

关键词： Strawberry edible coating cellulose nanocrystals pectin shelf life

Association between Helicobacterpylori, Epstein-Barr virus, human papillomavirus and gastric adenocarcinomas

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World Journal of Gastroenterology 2018年第43期24卷 4928-4938页

作者： Carolina Rosal Teixeira de souza Marcelli Carolini Alves Almeida Andre Salim Khayat Emerson Lucena da silva Paulo Cardoso soares Luiz Claudio Chaves Rommel Mario Rodriguez Burbano Laboratory of Human Cytogenetics Institute of Biological Sciences Federal University of Para Belem Para 66075-110 Brazil Oncology Research Center Federal University of Para Joao deBarros Barreto University Hospital Belem Para 66073-000Brazil Ophir Loyola Hospital Belem Para 66060-281 Brazil

AIM TO correlate Helicobacter pylori （H. pylorli）, EpsteinBarr virus （EBV） and human papillomavirus （HPV） with gastric cancer （GC） cases in Para State, *** Tissue samples were obtained from 302 gastric adenocarcinomas. A rapid urease test was used to detect the presence of H. pylori, and the presence of the cagA gene in the HP-positive samples was confirmed by PCR. An RNA in situ hybridization test designed to complement Eberl RNA was used to detect the presence of EBV in the samples, and the L1 region of HPV was detected using nested PCR. Positive HPV samples were genotyped and analyzed for E6 and E7 viral gene expression. Infections were also correlated with the clinical and pathological characteristics of the *** The majority of the 302 samples analyzed were obtained from men （65%） aged 55 years or older （67%） and were classified as the intestinal subtype （55%）. All three pathogens were found in the samples analyzed in the present study （H. pylori： 87%, EBV： 20%, HPV： 3%）. Overall, 78% of the H. pylori-positive （H. pylorl＋） samples were cagA＋（H. pylori-cagA＋）, and there was an association between the cytotoxic product of this gene and EBV. Coinfections of H. pylori-cagA＋ and EBV were correlated with the most advanced tumor stages. Although only 20% of the tumors were positive for EBV, infection with this virus was associated with distant metastasis. Only the HPV 16 and 18 strains were found in the samples, although no expression of the E6 and E7 oncoproteins was detected. The fundus of the stomach was the region least affected by the *** HPV was not involved in gastric tumorigenesis. Prophy- lactic and therapeutic measures against H. pylori and EBV may prevent the development of GC, especially the more aggressive forms.

关键词： Gastric cancer Gastric adenocarcinomas Microorganisms Helicobacter pylori cagA Epstein-Barr virus Human papillomavirus

Apparent soil electrical conductivity in the delineation of management zones for cocoa cultivation

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Information Processing in Agriculture 2022年第3期9卷 443-455页

作者： Samuel de Assis silva Railton Oliveira dos Santos daniel Marcal de Queiroz Juliao soares de souza Lima Levi Fraga Pajehua Caique Carvalho Medauar Center for Agricultural Sciences and Engineering Federal University of Espirito SantoAlegre CampusEspirito Santo 29500-000Brazil department of Agricultural Sciences Santa Cruz State UniversityIlheusBahia 45662-900Brazil department of Agricultural Engineering Federal University of Vic¸osaVic¸osaMinas Gerais 36570-900Brazil

Apparent electrical conductivity is an important parameter for describing the spatial vari-ability of physical and chemical attributes of the soil and for the delineation of manage-ment *** objective of this work is to outline management zones for the cocoa cultivation based on the spatial variability of the productivity and the apparent electrical conductivity(ECa)of the *** collection was performed in a regular sample grid con-taining 120 points in an area cultivated with cocoa trees,located in the municipality of Ilhe´us,state of Bahia,*** each sampling point(cocoa tree),soil samples were col-lected to determine chemical *** was measured for one year,counting,monthly,the number of fruits,which were classified into off-season cocoa,harvest and annual *** of the apparent electrical conductivity of the soil were performed at different times of the year using a portable conductivity *** data were analyzed using classical statistics and *** management zones were delin-eated using the fuzzy k-means *** ideal number of class was defined using the fuzziness performance index(FPI)and the entropy of the modified partition(MPE)*** Kappa coefficient was used to validate the management zones,assessing their agreement with the chemical attributes of the *** ECa of the soil values pre-sented moderate temporal variation,with maximum amplitude of 19.37 mS m1 and min-imum of 0.82 mS m1 between measurement periods;higher averages of the ECa coincided with the highest levels of water in the *** measurements of the ECa of the soil carried out in April and October showed greater correlation with the chemical attributes of the soil,with significant values for 11 and 8 of the 17 attributes evaluated,*** man-agement zones from the ECa measured in April showed:a)reduced number of classes;b)spatial continuity between classes,and;c)agreement from reasonable(kappa between

关键词： Theobroma cacao Precision agriculture Spatial variability Fuzzy k-means

Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

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World Journal of Methodology 2024年第1期14卷 114-123页

作者： Carolline Santos Miranda daiana Araujo Santana-Oliveira Isabela Lopes Vasques-Monteiro Nathan soares dantas-Miranda Jade Sancha de Oliveira Glauser Flavia Maria silva-Veiga Vanessa souza-Mello department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil

BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa,leading to low-grade inflammation,impaired mucosal integrity,and increased intestinal permeability,resulting in the migration of lipopolysaccharide(LPS)to other *** To evaluate the chronic effects(at 10 and 16 wk)of a high-fat diet(HFD)(with 50%energy as fat)on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 *** Forty adult male mice were divided into four nutritional groups,where the letters refer to the type of diet(control and HFD or HF)and the numbers refer to the period(in weeks)of diet administration:Control diet for 10 wk,HFD for 10 wk,control diet for 16 wk,and HFD for 16 *** sacrifice,biochemical,molecular,and stereological analyses were *** The HF groups were overweight,had gut dysbiosis,had a progressive decrease in occludin immunostaining,and had increased LPS *** progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the HF16 group,consistent with a completely disarranged intestinal ultrastructure after 16 wk of HFD *** Chronic HFD intake causes overweight,gut dysbiosis,and morphological and functional alterations of the intestinal barrier after 10 or 16 ***-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.

关键词： High-fat diet Intestine Ultrastructure Goblet cells Gut microbiota

Acromyrmex ameliae sp. n. （Hymenoptera： Formicidae）： A new social parasite of leaf-cutting ants in Brazil

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Insect Science 2007年第3期14卷 251-257页

作者： daNIVAL JOSE de souza ILKA MARIA FERNANdeS soares TEREZINHA MARIA CASTRO deLLA LUCIA Institut de Recherche sur la Biologie de 1'Insecte Université Francois Rabelais Tours France departamento de Biologia Animal Laboratório de Ecologia de Comunidades Departamento de Biologia Geral Universidade Federal de Vicosa MG 36570-000 Brazil

The fungus-growing ants （Tribe Attini） are a New World group of〉 200 species, all obligate symbionts with a fungus they use for food. Four attine taxa are known to be social parasites of other attines. Acromyrmex （ Pseudoatta） argentina argentina and Acromyrmex （Pseudoatta） argentina platensis （parasites of Acromyrmex lundi）, and Acromyrmex sp. （a parasite of Acromyrmex rugosus） produce no worker caste. In contrast, the recently discovered Acromyrmex insinuator （a parasite of Acromyrmex echinatior） does produce workers. Here, we describe a new species, Acromyrmex ameliae, a social parasite of Acromyrmex subterraneus subterraneus and Acromyrmex subterraneus brunneus in Minas Gerais, Brasil. Like A. insinuator, it produces workers and appears to be closely related to its hosts. Similar social parasites may be fairly common in the fungus-growing ants, but overlooked due to the close resemblance between parasite and host workers.

关键词： Acromyrmex leaf-cutting ants social evolution social parasitism

Recycling process and proto-kimberlite melt metasomatism in the lithosphere-asthenosphere boundary beneath the Amazonian Craton recorded by garnet xenocrysts and mantle xenoliths from the Carolina kimberlite

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Geoscience Frontiers 2022年第5期13卷 89-105页

作者： Fernanda Gervasoni Tiago Jalowitzki Marcelo Peres Rocha Ricardo Kalikowski Weska Eduardo Novais-Rodrigues Rodrigo Antonio de Freitas Rodrigues Yannick Bussweiler Elisa soares Rocha Barbosa Jasper Berndt Elton Luiz dantas Valmir da silva souza Stephan Klemme Centro de Engenharias Universidade Federal de Pelotas(UFPel)PelotasBrazil Programa de Pós-graduação em Geociências Universidade Federal do Rio Grande do SulPorto AlegreBrazil Programa de Pós-graduação em Geologia Instituto de GeociênciasUniversidade de Brasília(UnB)BrasíliaBrazil Faculdade de Geociências Universidade Federal do Mato Grosso(UFMT)CuiabáBrazil Institut für Geologie und Mineralogie Universität zu KölnGermany Faculdade de Ciências e Tecnologia Universidade Federal de Goiás(UFG)Aparecida de GoiâniaBrazil Institut für Mineralogie Universität MünsterMünsterGermany

Here we present new data on the major and trace element compositions of silicate and oxide minerals from mantle xenoliths brought to the surface by the Carolina kimberlite,Pimenta Bueno Kimberlitic Field,which is located on the southwestern border of the Amazonian *** also present Sr-Nd isotopic data of garnet xenocrysts and whole-rocks from the Carolina *** xenoliths are mainly clinopyroxenites and *** of the clinopyroxenites were classified as GPP–PP–PKP(garnet-phlogopite peridotite,phlogopite-peridotite,phlogopite-K-richterite peridotite)suites,and two clinopyroxenites(eclogites)and two garnetites are relicts of an ancient subducted *** and pressure estimates yield 855–1102℃ and 3.6–7.0 GPa,*** are enriched in light rare earth elements(LREE)(La_(N)/Yb_(N)=5–62;Ce_(N)/Sm_(N)=1–3;where N=primitive mantle normalized values),they have high Ca/Al ratios(10–410),low to medium Ti/Eu ratios(742–2840),and low Zr/Hf ratios(13–26),which suggest they were formed by metasomatic reactions with CO_(2)-rich silicate *** with high TiO_(2)(>2.0 wt.%),Al_(2)O_(3)(>12.0 wt.%),and FeOt(5.0–13.0 wt.%)resemble those found in the groundmass of kimberlites,lamproites and ***,phlogopite with low TiO_(2)(de)and PIC(phlogopite-ilmenite-clinopyorxene)*** GPP-PP-PKP suite of xenoliths,together with the clinopyroxene and phlogopite major and trace element signatures suggests that an intense proto-kimberlite melt metasomatism occurred in the deep cratonic lithosphere beneath the Amazonian *** Sr-Nd isotopic ratios of pyrope xenocrysts(G3,G9 and G11)from the Carolina kimberlite are characterized by high ^(143)Nd/^(144)Nd(0.51287–0.51371)and eNd(+4.55 to+20.85)accompanied with enriched ^(87)Sr/^(86)Sr(0.70405–0.71098).These results suggest intera

关键词： Carolina kimberlite K-rich mantle xenoliths Ancient subducted slab Proto-kimberlite melt metasomatism

The Current and Future Role of Drugs and Probiotics in the Management of Inflammatory Bowel Disease

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Journal of Biosciences and Medicines 2015年第8期3卷 76-85页

作者： maric.lma da silva soares de souza Sandra Maria Barbalho Ricardo de Alvares Goulart Antonely de Cássio Alves de Carvalho department of Biochemistry and Pharmacology School of Medicine University of Marília Marília S&atilde o Paulo Brazil department of Biochemistry and Pharmacology School of Medicine University of Marília and Food Technology School (FATEC) Marília S&atilde o Paulo Brazil department of Gastroenteology University Hospital-UNIMAR Marília S&atilde o Paulo Brazil Diagnostic Center in Gastroenterology Ibaiti Paraná Brazil

Inflammatory Bowel Disease (IBD) and other auto-inflammatory disorders are conditions caused by chronic and persistent intestinal inflammation, which is mainly represented by Ulcerative Colitis (UC) or Crohn’s Disease (CD), both of which typically arise as chronic inflammation of the gastrointestinal mucosa. Doctor’s main focus for treating IBD is the decrease on inflammation processes when the disease is active and promotes the maintenance of inflammation at normal levels. However, to deal with these two parameters is not easy, because the drugs usually used are related to several side effects and they are not totally effective to reduce inflammation and to keep the remission. Authors agree that the therapy approach should include drugs, dietary intervention and use of alternative substances as probiotics. The standard treatment approach for patients with CD and UC is traditionally done with the use of anti-inflammatory agents, like corticosteroids, mesalamine, immunomodulators such as azathioprine and methotrexate, and biologic agents such as infliximab and others. Probiotics are known as viable micro-organisms (or live microorganisms or live microbial dietary supplements) that may promote beneficial physiologic or therapeutic properties when used correctly. The interest in the role of these substances in improving human health is the reason of several researches. They may be used to help the immune system, to control gastro-intestinal infections and to prevent several diseases. Prebiotics, symbiotics, and probiotics may be helpful as adjuvants in the treatment of IBD. Probiotics are normally well-tolerated and are associated with few side effects which are undoubtedly important in the treatment of any disease.

关键词： Inflammatory Bowel Diseases Immunomodulators Biological Agents Probiotics

Novel Protease from Aspergillus tamarii URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

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Advances in Enzyme Research 2016年第4期4卷 125-143页

作者： Osmar soares da silva Rodrigo Lira de Oliveira Cristina Maria souza-Motta Ana Lúcia Figueiredo Porto Tatiana souza Porto Northeast Biotechnology Network/RENORBIO Federal Rural University of Pernambuco (UFRPE) Recife Brazil Academic Unity of Garanhuns/UAG Federal Rural University of Pernambuco (UFRPE) Garanhuns Brazil department Mycology Federal University of Pernambuco (UFPE) Recife Brazil department of Morphology Physiology Animal/DMFA Federal Rural University of Pernambuco (UFRPE) Recife Brazil

This study reports the protease production from Aspergillus tamarii using agroindustrial residues as substrate for solid-state fermentation (SSF) and biochemical characterization. The highest protease production was obtained using wheat bran as substrate at 72 h fermentation with maximum proteolytic activity of 401.42 U/mL, collagenase of 243.0 U/mL and keratinase of 19.1 U/mL. The protease exhibited KM = 18.7 mg/mL and Vmax = 28.5 mg/mL/min. The optimal pH was 8.0 and stable in a wide pH range (5.0 - 11.0) during 24 h. The optimum temperature was 40°C. The proteolytic activity was inhibited by Cu2+ (33.98%) and Hg2+ (22.69%). The enzyme was also inhibited by PMSF (65.11%), indicating that is a Serine Protease. These properties suggest that alkaline protease from A. tamarii URM4634 is suitable for application in food industries and leather processing. Additionally, the present findings opened new vistas in the utilization of wheat bran and other effective agroindustrial wastes as substrates for SSF.

关键词： Protease Aspergillus tamarii Biochemical Characterization Solid-State Fermentation Agroindustrial Waste

Productivity and Dry Matter Accumulation of Sugarcane Crop under Irrigation and Nitrogen Application at Rio Verde GO, Brazil

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American Journal of Plant Sciences 2015年第14期6卷 2374-2384页

作者： Alefe Viana souza Bastos Renato Campos de Oliveira Nelmício Furtado da silva Marconi Batista Teixeira Frederico Antonio Loureiro soares Edson Cabral da silva Instituto Federal Goiano (IFgoiano) Rio Verde Brazil

Dry matter production and productivity of stem currently are being widely studied in sugarcane, reinforcing the study in question, which aims to assess the accumulation of dry matter of the aerial segment and the productivity of stems of sugarcane crops within the first cycle, at different levels of water replacement (WR) with and without nitrogen fertilization, through a subsurface drip irrigation system. The assay was conducted in the experimental area of the Federal Institut Goiano, Campus Rio Verde, GO, Brazil, in a dystroferric Rhodic Hapludox soil, cerrado phase (savannah), and comprised experimental splits of three furrows with an 8-meter long double row. Experimental design consisted of randomized blocks in a 5 × 2 factorial array, with four replications. Evaluated factors comprised five levels of WR (100%, 75%, 50%, 25% and 0% of field capacity), with and without the application of nitrogen (0 and 100 kg·ha-1 urea). Harvest occurred in May 2013 and stem productivity (SP), productivity of pointers (PP), productivity of straw (PS), harvest index (HI), dry matter of stem (DMS), dry matter of pointers (DMP), the relationship between dry matter of pointer and dry matter of stem (DMP/DMS) and total dry matter of the aerial segment (TDM) were determined. The variables SP, PP, DMS and DMP had a linear growth in proportion to WR increase, whereas HI and DMP/DMS adjusted to a quadratic model. Nitrogen fertilization affected positively the variables SP, HI, DMS and DMP/DMS and occurred interaction to TDM;also increasing the productivity stem and the harvest index.

关键词： Sugarcane Ratoon Water Replacement Nitrogen Fertilizer

development and implementation of an automated system to aid laboratory diagnosis using image processing

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Journal of Biomedical Science and Engineering 2013年第5期6卷 579-585页

作者： Alvaro Manoel de souza soares Marco Rogério da silva Richetto Joao Bosco Goncalves Pedro Paulo Leite do Prado department of Mechanical Engineering University of TaubateTaubateBrasil department of Electrical Engineering University of TaubateTaubateBrasil

The objective of this work is to provide an automatic system to count white blood cells in a blood smear. To do so an experiment was assembled, composed by a standard microscope with two step motors coupled to its knobs in order to move the microscope in x and y directions and a web cam which was mounted in the top of the microscope responsible for to acquire images from the smear. The step motors and the web cam are controlled by a microcomputer PC standard via software developed indelphi. The motors use the parallel port to communicate with the PC and the camera use the USB port. The main idea is to set an initial point into the smear and the automated system will carry over the smear acquiring images (frames with 640 × 480 pixels) and counting the white blood cells encountered. The double histogram threshold technique is implemented to initially exclude the red cells from the image leaving only the white ones. Preliminaries results are obtained and show that the system is quite fast and has a good capacity of selection, even when different kinds of smear are used.

关键词： Image Processing Robotics Automation Pattern Recognition