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Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

BioDesign Research 2021年第1期2021卷 79-95页

作者： Rubén Mateos-Fernández Elena Moreno-Giménez Silvia Gianoglio Alfredo Quijano-Rubio Jose Gavaldá-garcía Lucía Estellés Alba Rubert JoséLuis Rambla Marta Vazquez-Vilar Estefanía Huet Asunción Fernández-del-Carmen Ana Espinosa-Ruiz Mojca Juteršek Sandra Vacas Ismael Navarro Vicente Navarro-Llopis Jaime Primo Diego Orzáez Institute for Plant Molecular and Cell Biology(IBMCP) Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Politécnica de Valencia(UPV)ValenciaSpain Jaume I University Castellon de la PlanaSpain Department of Biotechnology and Systems Biology National Institute of BiologyLjubljanaSlovenia Jožef Stefan International Postgraduate School LjubljanaSlovenia Centro de Ecología Química Agrícola Instituto Agroforestal del MediterráneoUniversitat Politècnica de ValènciaValenciaSpain Ecología Y Protección Agrícola SL ValenciaSpain

Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in ***,we describe the engineering of transgenic plants producing(Z)-11-hexadecenol(Z11-16OH)and(Z)-11-hexadecenyl acetate(Z11-16OAc),two main volatile components in many Lepidoptera sex pheromone *** assembled multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them into Nicotiana benthamiana *** constructs contained the Amyelois transitella AtrΔ11 desaturase gene,the Helicoverpa armigera fatty acyl reductase HarFAR gene,and the Euonymus alatus diacylglycerol acetyltransferase EaDAct gene in different *** the pheromone-producing plants showed dwarf phenotypes,the severity of which correlated with pheromone *** but one of the recovered lines produced high levels of Z11-16OH,but very low levels of Z11-16OAc,probably as a result of recurrent truncations at the level of the EaDAct *** one plant line(SxPv1.2)was recovered that harboured an intact pheromone pathway and which produced moderate levels of Z11-16OAc(11.8μg g^(-1) FW)and high levels of Z11-16OH(111.4μg g^(-1)).Z11-16OAc production was accompanied in SxPv1.2 by a partial recovery of the dwarf ***1.2 was used to estimate the rates of volatile pheromone release,which resulted in 8.48 ng g-1 FW per day for Z11-16OH and 9.44 ng g^(-1 )FW per day for *** results suggest that pheromone release acts as a limiting factor in pheromone biodispenser strategies and establish a roadmap for biotechnological improvements.

关键词： release dwarf transformed

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基于PSPL调研法解读面状公共空间和公共生活——以西班牙马德里蒂尔索德莫利纳广场为例

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建筑与文化 2017年第1期 130-132页

作者：孙士博徐熙焱艾斯特.伊格拉斯.加西亚刘伟北京林业大学西班牙马德里理工大学西班牙马德里自治大学北京林业大学园林学院

以西班牙首都马德里市中心蒂尔索德莫利纳广场为例,阐述了面状公共空间的概念和高品质面状空间的特点,介绍其发展历程的同时,运用扬·盖尔提出的PSPL方法,实地调查了该面域开放空间的特征,记录了冬夏两季共六天、每天四个时间段的市民... 详细信息

以西班牙首都马德里市中心蒂尔索德莫利纳广场为例,阐述了面状公共空间的概念和高品质面状空间的特点,介绍其发展历程的同时,运用扬·盖尔提出的PSPL方法,实地调查了该面域开放空间的特征,记录了冬夏两季共六天、每天四个时间段的市民公共生活情况,并对调研结果进行数据处理和分析。通过对该场地的整体分析,总结出一个有品质的面状城市公共空间应具备的特质及其与公共生活质量的相关性,为我国当前城市面状公共空间建设提供有益的借鉴与参考。

关键词：面状公共空间空间品质公共生活扬·盖尔PSPL调研法西班牙城市广场

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口服纳多洛尔治疗婴幼儿血管瘤及普萘洛尔引起的睡眠障碍

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中国口腔颌面外科杂志 2015年第6期 529-529页

作者： Bernabeu-Wittel J Narváez-Moreno B de la Torre-garcía JM

口服普萘洛治疗婴幼儿血管瘤(IH)已被证明安全有效,其副作用包括睡眠障碍等。该文对普萘洛尔治疗过程中出现睡眠障碍导致治疗中断的部分患儿改用口服纳多洛尔治疗,并对该药的疗效及安全性进行评估。对三级儿童医院2008年7月-2013年3月... 详细信息

口服普萘洛治疗婴幼儿血管瘤(IH)已被证明安全有效,其副作用包括睡眠障碍等。该文对普萘洛尔治疗过程中出现睡眠障碍导致治疗中断的部分患儿改用口服纳多洛尔治疗,并对该药的疗效及安全性进行评估。对三级儿童医院2008年7月-2013年3月间接受普萘洛尔治疗的97例IH患儿进行回顾分析。结果:9(9.3%)例患儿出现睡眠障碍,7例中断治疗,改服纳多洛尔,5(71%)例患儿症状缓解,1例4个月后再次出现睡眠障碍。结论:由于样本量过小,

关键词：普萘洛尔纳多洛尔婴幼儿血管瘤睡眠障碍

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Effect of electromagnetic interaction on microstructure and corrosion resistance of 7075 aluminium alloy during modified indirect electric arc welding process

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Transactions of Nonferrous Metals Society of China 2019年第3期29卷 473-484页

作者： J. S. REYNA-MONTOYA M. A. garcIA-RENTERIA V. L. CRUZ-HERNANDEZ F. F. CURIEL-LOPEZ L. R. DZIB-PEREZ L. A. FALCON-FRANCO Faculty of Metallurgy Autonomous University of CoahuilaCarretera 57 Km. 5 CP 25720 Monclova Coahuila México Instituto de Investigación en Metalurgia y Materiales Universidad Michoacana de San Nicolás de Hidalgo A. P. 888 CP 58030 Morelia Michoacán México Centre for Corrosion Research Autonomous University of CampecheAv. Agustín Melgar s/n Col. Buenavista CP 24039 Campeche Cam México

The effects of applying an electromagnetic interaction of low intensity (EMILI) on the microstructure and corrosion resistance of 7075-T651 Al alloy plates (13 mm in thickness) during modified indirect electric arc (MIEA) welding were investigated. The welding process was conducted in a single pass with a heat input of ~1.5 kJ/mm. The microstructural observations of the welds were correlated with the effect of EMILI on the local mechanical properties and the corrosion resistance in natural seawater by means of microhardness measurements and electrochemical impedance spectroscopy, respectively. Microstructural characterization of the welds revealed a grain refinement in the weld metal due to the electromagnetic stirring induced by EMILI of 3 mT during welding. In addition, observations in the scanning electron microscope showed that the precipitation of Cu-rich phases and segregation of eutectics were reduced in the heat affected zone (HAZ) also as an effect of EMILI. The high corrosion dissolution of the 7075-T651 welds in natural seawater and the extent of overaging in the HAZ were reduced when welding with EMILI of 3 mT. Thus, EMILI along with the MIEA technique may lead to welded joints with better microstructural characteristics, improved mechanical properties in the HAZ and reduced electrochemical activity.

关键词： 7075-T651 Al alloy modified indirect arc welding microstructural characterization corrosion resistance electromagnetic interaction

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Validation of a new scoring system: Rapid assessment faecal incontinence score

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World Journal of Gastrointestinal Surgery 2015年第9期7卷 203-207页

作者： Fernando de la Portilla Arantxa Calero-Lillo Rosa M Jiménez-Rodríguez Maria L Reyes Manuela Segovia-González María Victoria Maestre Ana M garcía-Cabrera Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD o Ciberehd) Instituto de Salud Carlos Ⅲ Department of General and Digestive Surgery Colorrectal Surgery Unit"Virgen del Rocío" University Hospital/IBi S/CSIC/University of Seville Department of Economics Quanti- tative MethodsUniversity Pablo Olavide of Seville

AIM: To implement a quick and simple test- rapid assessment faecal incontinence score(RAFIS) and show its reliability and ***: From March 2008 through March 2010, we evaluated a total of 261 consecutive patients, including 53 patients with faecal incontinence. Demographic and comorbidity information was collected. In a single visit, patients were administered the RAFIS. The results obtained with the new score were compared with those of both Wexner score and faecal incontinence quality of life scale(FIQL) questionnaire. The patient withoutinfluence of the surgeon completed the test. The role of surgeon was explaining the meaning of each section and how he had to fill. Reliability of the RAFIS score was measured using intra-observer agreement and Cronbach's alpha(internal consistency) coefficient. Multivariate analysis of the main components within the different scores was performed in order to determine whether all the scores measured the same factor and to conclude whether the information could be encompassed in a single factor. A sample size of 50 patients with faecal incontinence was estimated to be enough to detect a correlation of 0.55 or better at 5% level of significance with 80% ***: We analysed the results obtained by 53 consecutive patients with faecal incontinence(median age 61.55 ± 12.49 years) in the three scoring systems. A total of 208 healthy volunteers(median age 58.41 ± 18.41 years) without faecal incontinence were included in the study as negative controls. Pearson's correlation coefficient between "state" and "leaks" was excellent(r = 0.92, P < 0.005). Internal consistency in the comparison of "state" and "leaks" yielded also excellent correlation(Cronbach's α = 0.93). Results in each score were compared using regression analysis and a correlation value of r = 0.98 was obtained with Wexner score. As regards FIQL questionnaire, the values of "r " for the different subscales of the questionnaire were: "lifestyle" r =-0.87, "c

关键词： Faecal incontinence Measure Score Test Faecal inco

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G-StarRAW总部

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城市环境设计 2014年第6期83卷 114-121页

作者： Katrien van Dijk Michel van de Kar Tjeerd van de Sandt Saskia Simon Mario Rodriguez Marina Cogliani Jung-Won Yoon Saskia Simon Marlies Boterman Marina Coglianiv Karolina Czeczek Green van Gogh Sarah Moylan Mafalda Rangel Tjeerd van de Sandt Richard Hollington Ⅲ Tjeerd van de Sandt Fred Awty Philippe Braun Kaveh Dabiri Katrien van Dijk Hans Hammink mariano Sagasta Koen Stockbroekx

G-Star RAW新总部位于阿姆斯特丹Zuid-Oost工业区内的A10路旁,其单栋建筑集G-Star RAW各类不同设施于一身,旨在刺激各部门间的互动合作。横向的总部大楼长140m,面积达27 500m2,由一个容纳G-Star RAW各个核心部门的"创作核"组成,外围则... 详细信息

G-Star RAW新总部位于阿姆斯特丹Zuid-Oost工业区内的A10路旁,其单栋建筑集G-Star RAW各类不同设施于一身,旨在刺激各部门间的互动合作。横向的总部大楼长140m,面积达27 500m2,由一个容纳G-Star RAW各个核心部门的"创作核"组成,外围则是办公室、停车位和配套设施。对比强烈的材料突显出核心创作区和外环支援活动之间的区别──外环是体量庞大的黑色

关键词： Star G-StarRAW

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Improving ITS sequence data for identification of plant pathogenic fungi

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Fungal Diversity 2014年第4期67卷 11-19页

作者： R.Henrik Nilsson Kevin D.Hyde Julia Pawlowska Martin Ryberg Leho Tedersoo Anders Bjornsgard Aas Siti A.Alias Artur Alves Cajsa Lisa Anderson Alexandre Antonelli A.Elizabeth Arnold Barbara Bahnmann Mohammad Bahram Johan Bengtsson-Palme Anna Berlin Sara Branco Putarak Chomnunti Asha Dissanayake Rein Drenkhan Hanna Friberg Tobias Guldberg Froslev Bettina Halwachs Martin Hartmann Beatrice Henricot Ruvishika Jayawardena Ari Jumpponen Havard Kauserud Sonja Koskela Tomasz Kulik Kare Liimatainen Bjorn D.Lindahl Daniel Lindner Jian-Kui Liu Sajeewa Maharachchikumbura Dimuthu Manamgoda Svante Martinsson Maria Alice Neves Tuula Niskanen Stephan Nylinder Olinto Liparini Pereira Danilo Batista Pinho Teresita M.Porter Valentin Queloz Taavi Riit Marisol Sánchez-garcía Filipe de Sousa Emil Stefańczyk Mariusz Tadych Susumu Takamatsu Qing Tian Dhanushka Udayanga Martin Unterseher Zheng Wang Saowanee Wikee Jiye Yan Ellen Larsson Karl-Henrik Larsson Urmas Koljalg Kessy Abarenkov Department of Biological and Environmental Sciences University of GothenburgBox 46140530 GothenburgSweden Institute of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Department of Plant Systematics and Geography Faculty of BiologyUniversity of WarsawAl.Ujazdowskie 400-478 WarsawPoland Department of Organismal Biology Uppsala UniversityNorbyvagen 18D75236 UppsalaSweden Institute of Ecology and Earth Sciences University of TartuLai 40Tartu 51005Estonia Microbial Evolution Research Group University of OsloBlindernveien 310371 OsloNorway Institute of Biological Sciences University of Malaya50603 Kuala LumpurMalaysia Department of Biology CESAMUniversity of Aveiro3810-193 AveiroPortugal School of Plant Sciences The University of Arizona1140 E South Campus DriveForbes 303TucsonAZ 85721USA Laboratory of Environmental Microbiology Institute of Microbiology ASCRVídeňská108314220 Prague 4Czech Republic Department of Infectious Diseases Institute of BiomedicineSahlgrenska AcademyUniversity of GothenburgGuldhedsgatan 1041346 GoteborgSweden Department of Forest Mycology and Plant Pathology Swedish University of Agricultural SciencesBox 702675007 UppsalaSweden University of California at Berkeley 321 Koshland Hall University of CaliforniaBerkeleyCA 94720-3102USA Agriculture and Forestry Sciences Beijing 100097China Institute of Forestry and Rural Engineering Estonian University of Life SciencesKreutzwaldi551014 TartuEstonia Natural History Museum of Denmark Øster Voldgade 5-71350 København KDenmark Institute for Genomics and Bioinformatics Graz University of Technology8010 GrazAustria Core Facility Bioinformatics Austrian Centre of Industrial Biotechnology8010 GrazAustria Forest Soils and Biogeochemistry Swiss Federal Research Institute WSLZuercherstrasse 1118903 BirmensdorfSwitzerland Molecular Ecology Institute for Sustainability SciencesAgroscopeReckenholzstrasse 191

Plant pathogenic fungi are a large and diverse assemblage of eukaryotes with substantial impacts on natural ecosystems and human *** taxa often have complex and poorly understood life cycles,lack observable,discriminatory morphological characters,and may not be amenable to in vitro *** a result,species identification is frequently ***(DNA sequence)data have emerged as crucial information for the taxonomic identification of plant pathogenic fungi,with the nuclear ribosomal internal transcribed spacer(ITS)region being the most popular ***,international nucleotide sequence databases are accumulating numerous sequences of compromised or low-resolution taxonomic annotations and substandard technical quality,making their use in the molecular identification of plant pathogenic fungi *** we report on a concerted effort to identify high-quality reference sequences for various plant pathogenic fungi and to re-annotate incorrectly or insufficiently annotated public ITS sequences from these fungal lineages.A third objective was to enrich the sequences with geographical and ecological *** results-a total of 31,954 changes-are incorporated in and made available through the UNITE database for molecular identification of fungi(http://***),including standalone FASTA files of sequence data for local BLAST searches,use in the next-generation sequencing analysis platforms QIIME and mothur,and related *** present initiative is just a beginning to cover the wide spectrum of plant pathogenic fungi,and we invite all researchers with pertinent expertise to join the annotation effort.

关键词： Phytopathogenic fungi Molecular identification ITS Taxonomy Annotation

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加速度计和7d体力活动回顾评估血压昼夜节律模式与体育锻炼程度

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中华高血压杂志 2014年第8期22卷 798-798页

作者： garcía-Ortiz L Recio-Rodríguez JI Puig-Ribera A Lema-Bartolomé J Ibáez-Jalón E González-Viejo N Guenaga-Saenz N Agudo-Conde C Patino-Alonso MC Gomez-Marcos MA EVIDENT Group

客观测量和自我评估的体力活动与24h动态血压昼夜节律的相关性尚不完全清楚。方法：选取健康人群进行横断面队列研究。研究者从EVIDENT研究中选取1345例患者[年龄（55±14）岁;女性占59.3%]。采用7d体力活动回顾（physical activity rec... 详细信息

客观测量和自我评估的体力活动与24h动态血压昼夜节律的相关性尚不完全清楚。方法：选取健康人群进行横断面队列研究。研究者从EVIDENT研究中选取1345例患者[年龄（55±14）岁;女性占59.3%]。采用7d体力活动回顾（physical activity recall,PAR）问卷[代谢当量/（h·周）]和活动变化记录仪-GT3X加速度计（次/min）计算7d的体力活动;用桡动脉压力计测量动态动脉血压。结果：加速度计和7dPAR显示,

关键词：体力活动血压昼夜节律模式加速度计自我评估体育锻炼动态血压昼夜节律队列研究健康人群

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南墨西哥结核病高流行社区中高危人群在传播结核分支杆菌中的作用

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国际结核病与肺部疾病杂志 2000年第4期 112-116页

作者： Ma.deLourdesgarcía-garcía M.Palacios-Martínez A.Ponce-de-León Ma.E.Jiménez-Corona A.Jiménez-Corona S.Balandrano-Campos H.Olivera-Díaz J.L.Valdespino-Gómez P.M.Small 王海东 Instituto Nacional de Salud Publica Cuemavaca Instituto Nacional de la Nutticion Salvador Zubiron Mexico City Instituto Nacional de Diagnostico y Referencia Epidemiologicos Mexico City Mexico Stanford University Stanford Califomia USA

背景:高结核病流行的南墨西哥社区。目的:为描述以DOTS为基础的控制规程地区结核病传播的动态特征。设计:以社区为基础筛选1995年3月1日至1996年8月31日期间有慢性咳嗽者。登记、会见痰抗酸杆菌(AFB)阳性的病人,并做分支杆菌培养和指纹... 详细信息

背景:高结核病流行的南墨西哥社区。目的:为描述以DOTS为基础的控制规程地区结核病传播的动态特征。设计:以社区为基础筛选1995年3月1日至1996年8月31日期间有慢性咳嗽者。登记、会见痰抗酸杆菌(AFB)阳性的病人,并做分支杆菌培养和指纹。对所有做DNA指纹的人进行详细访问。结果:在进行抗酸杆菌涂片的1 424人中,有124人得到微生物学确认。获得95名病例的细菌DNA指纹,其中38例是成簇的。最大的一簇包括集中在几个未登记酒吧的7名社会网络成员。结论:这个以人群为基础的分子流行病学研究表明,在社会网络内的传播中,四分之一很快地发展成为疾病。这些观察对在卫生区域内实施WHO解释的DOTS规范的结核病控制措施的潜在利益提出了疑问。

关键词：结核病分子流行病学控制 IS6110 墨西哥高危人群

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Adequate antiplatelet regimen in patients on chronic anti-vitamin K treatment undergoing percutaneous coronary intervention

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World Journal of Cardiology 2011年第11期3卷 367-373页

作者： Salvatore Brugaletta Victoria Martin-Yuste Ignacio Ferreira-González Clarissa Cola Luis Alvarez-Contreras Marta De Antonio Xavier garcia-Moll Joan garcía-Picart Vicens Martí Jordi Balcells-Iranzo Manel Sabaté Thorax Institute Department of Car-diology Hospital Clinic Epidemiology Unit Department ofCardiology Vall d'Hebron Hospital Center of Epidemiology andPublic Heath Interventional Cardiology Unit University Hospital of Saint Paul Department of Cardiology University Hos-pital of Saint Paul

AIM: To investigate the impact of dual antiplatelet therapy (DAT) in patients on antivitamin K (AVK) regimen requiring percutaneous coronary intervention (PCI).METHODS: Between February 2006 and February 2008, 138 consecutive patients under chronic AVK treatment were enrolled in this registry. Of them, 122 received bare metal stent implantation and 16 received drug elutingstent implantation. The duration of DAT, on top of AVK treatment, was decided at the discretion of the clinician. Adequate duration of DAT was def ined according to type of stent implanted and to its clinical indication. RESULTS: The baseline clinical characteristics of patients reflect their high risk, with high incidence of comorbid conditions (Charlson score ≥ 3 in 89% of the patients). At a mean follow-up of 17 ± 11 mo, 22.9% of patients developed a major adverse cardiac event (MACE): 12.6% died from cardiovascular disease and almost 6% had an acute myocardial infarction. Major hemorrhagic events were observed in 7.4%. Adequate DAT was obtained in only 44% of patients. In the multivariate analysis, no adequate DAT and Charlson score were the only independent predictors of MACE (both P = 0.02). CONCLUSION: Patients on chronic AVK therapy represent a high risk population and suffer from a high MACE rate after PCI. An adequate DAT regimen and absence of comorbid conditions are strongly associated with better clinical outcomes.

关键词： Hemorrhagic risk Anti-vitamin K treatment Anti-platelet therapy Percutaneous coronary intervention

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