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Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

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Journal of Pharmaceutical Analysis 2019年第4期9卷 254-258页

作者： Yang Huang Tiejie Wang marianne fillet Jacques Crommen Zhengjin Jiang Institute of Pharmaceutical Analysis College of Pharmacy Jinan University Guangzhou 510632 China Shenzhen Institute for Drug Control Shenzhen China Department of Pharmaceutics State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China Laboratory for the Analysis of Medicine Department of Pharmaceutical Sciences CIRM University of Liege CHU B36 B-4000 Liege Belgium

Tea is a widely consumed beverage and has many important physiological properties and potential health benefits. In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was fully validated and successfully applied to the determination of these amino acids in six different tea samples. Good linearity (r ≥ 0.993), precision (RSDs≤ 2.99%), accuracy (91.95%-107.09%) as well as good sample stability were observed. The limits of detection ranged from 1.42 to 14.69 ng/mL, while the limits of quantification were between 4.53 and 47.0 ng/mL. The results indicate that the contents of the 11 amino acids in the six different tea samples are greatly influenced by the degree of fermentation. The proposed SFC-MS method shows a great potential for further investigation of tea varieties.

关键词： Amino acids Teas Supercritical fluid chromatography with single quadrupole mass spectrometry (SFCMS)

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Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS

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Journal of Pharmaceutical Analysis 2019年第4期9卷 259-265页

作者： Valentin Ion Caroline Legoff Etienne Cavalier Pierre Delanaye Anne-Catherine Servais Daniela-Lucia Muntean marianne fillet Laboratory for the Analysis of Medicines (LAM) Center for Interdisciplinary Research on Medicines (CIRM) University of Liege Liege Belgium Analytical Chemistry and Drug Analysis Department Faculty of Pharmacy University of Medicine Pharmacy Science and Technology from Tirgu Mures 540139 Tirgu Mures Romania Department of Clinical Chemistry Center for Interdisciplinary Research on Medicines (CIRM) University of Liege CHU Sart-Tilman Liege Belgium Department of Nephrology-Dialysis-Transplantation University of Liege CHU Sart-Tilman Liege Belgium

One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN■ device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days. By collecting only 10 mL of blood, iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250 mg/mL. Due to the analyte stability in VAMS for up to 12 days, this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis.

关键词： Microsampling Iohexol VAMS LC-MS/MS HemaPEN

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Release of Cardiac Biomarkers during a Cycling Race

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World Journal of Cardiovascular Diseases 2016年第8期6卷 285-294页

作者： Caroline Le Goff Jean-François Kaux Stéphanie D’Otreppe Sébastien Goffaux marianne fillet Jean-Paul Chapelle Etienne Cavalier Department of Clinical Chemistry University of Liège Liège Belgium Department of Physical Medicine and Sport Traumatology SPORTS2 FIFA Medical Centre of Excellence University of Liège Liège Belgium Department of Analytical Pharmaceutical Chemistry University of Liège Liège Belgium

Objectives: Over the past two decades, a large interest in cardiac marker elevations has developed in endurance sports events. The intense effort is not without risk. We aimed to see if the relatively cardiospecific biomarkers could show the damage on cardiac muscle cells. Methods: Fourteen cyclists were recruited for an international race (177 km). We studied different cardiac biomarkers, renal function markers and blood cytology. The subjects were submitted to three blood test: one before (T0), one just after (T1) and the last one 3 hours after the race (T3). Results: Blood cytology markers, namely erythrocytes, hemoglobin, hematocrit, and average hemoglobin concentration, were found to evolve in a similar way. Renal function markers, such as creatinin, cystatin C and uric acid, showed a post effort increase that might be related to renal blood flow depletion during exercise. Cardiac and muscular markers were all increased at T1. Conclusions: Physiological stress induced by an international cycling race certainly has consequences on cardiac muscle cells. Fortunately, those blood concentration variations are more representative of a transitional state, due to an imbalance created by an intense aerobic effort maintained during several hours, rather than an irreversible injury.

关键词： Cycling Cardiac Biomarkers Stress Biomarkers Renal Function Markers

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