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A molecular phenology scale of grape berry development

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Horticulture Research 2023年第5期10卷 72-84页

作者： Giovanni Battista Tornielli Marco Sandri marianna Fasoli Alessandra amato Mario Pezzotti Paola Zuccolotto Sara Zenoni department of Biotechnology University of VeronaStrada Le Grazie 1537134 VeronaItaly Big&Open data Innovation Laboratory University of BresciaC.da S.Chiara 5025122 BresciaItaly

Fruit growth and development consist of a continuous succession of physical,biochemical,and physiological changes driven by a genetic program that dynamically responds to environmental *** recognizable stages over the whole fruit lifetime represents a fundamental requirement for research and fruit crop *** is especially relevant in perennial crops like grapevine(Vitis vinifera L.)to scale the development of its fruit across genotypes and growing *** this work,molecular-based information from several grape berry transcriptomic datasets was exploited to build a molecular phenology scale(MPhS)and to map the ontogenic development of the *** proposed statistical pipeline consisted of an unsupervised learning procedure yielding an innovative combination of semiparametric,smoothing,and dimensionality reduction *** transcriptomic distance between fruit samples was precisely quantified by means of the MPhS that also enabled to highlight the complex dynamics of the transcriptional program over berry development through the calculation of the rate of variation of MPhS stages by *** MPhS allowed the alignment of time-series fruit samples proving to be a complementary method for mapping the progression of grape berry development with higher detail compared to classic time-or phenotype-based approaches.

关键词： crops yielding lifetime

Anti-amyloid antibodies in Alzheimer’s disease: what did clinical trials teach us?

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Neural Regeneration Research 2025年第4期20卷 1092-1093页

作者： danko Jeremic Lydia Jiménez-díaz Juan d.Navarro-López NeuroPhysiology&Behavior Lab University of Castilla-La ManchaBiomedical Research Center(CRIB)School of Medicine of Ciudad RealCiudad RealSpain

Although many causes of Alzheimer’s disease(Ad)may exist,both the original amyloid cascade and tau hypotheses posit that abnormal misfolding and accumulation of amyloid-β(Aβ)and tau protein is the central event causing the ***,that conclusion could be only partly true,and there is conflicting evidence about the role of both proteins in Ad,being able to precede and influence one *** researchers argue that these proteins are mere executors rather than primary causes of ***,there have been continuing refinements of both hypotheses,with alternative explanations proposed.Aβand tau proteins may be independently involved in specific neurotoxic pathways;yet there may be other crucial processes going on in early ***,accumulating evidence suggests that Aβand tau act synergistically,rather than additively in disease onset(Jeremic et al.,2021,2023a).

关键词： amyloid Alzheimer additive

Remaking a connection:molecular players involved in post-injury synapse formation

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Neural Regeneration Research 2025年第6期20卷 1719-1720页

作者： diogo Tomé Ramiro d.Almeida iBiMEd-Institute of Biomedicine Department of Medical SciencesUniversity of AveiroAveiroPortugal CNC-Center for Neuroscience and Cell Biology University of CoimbraCoimbraPortugal

Functional recovery from central nervous system(CNS)trauma depends not only on axon regeneration or compensatory sprouting of uninjured fibers but also on the ability of newly grown axons to establish functional synapses with appropriate *** several studies have successfully promoted long-distance axonal regeneration in distinct CNS injury models,none of them have resulted in a viable therapeutic approach for patient recovery.A possible reason may be the lack of new synaptogenesis for reestablishing the circuitry lost after ***,we discuss how our understanding of the mechanisms that instruct synapse formation in the injured nervous system may contribute to the design of new strategies to promote functional restoration in traumatic CNS disorders.

关键词： viable injury instru

dihydropyrimidine dehydrogenase polymorphisms in patients with gastrointestinal malignancies and their impact on fluoropyrimidine tolerability: Experience from a single Italian institution

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 101-109页

作者： Mariarosaria d'amato Gennaro Iengo Nicola Massa Chiara Carlomagno department of Oncology Ospedale San Rocco ASL CasertaSessa Aurunca 81037CampaniaItaly department of Clinical Medicine and Surgery University of Naples Federico IINaples 80131CampaniaItaly

BACKGROUNd Fluoropyrimidines are metabolized in the liver by the enzyme dihydropyrimidine dehydrogenase(dPd),encoded by the dPYd *** 7%of the European population is a carrier of dPYd gene polymorphisms associated with reduced dPd enzyme *** To assess the prevalence of dPYd polymorphisms and their impact on fluoropyrimidine tolerability in Italian patients with gastrointestinal *** A total of 300 consecutive patients with a diagnosis of gastrointestinal malignancy and treated with a fluoropyrimidine-based regimen were included in the analysis and divided into two cohorts:(1)149 patients who started fluoropyrimidines after dPYd testing;and(2)151 patients treated without dPYd *** the patients in cohort A,15%tested only the dPYd2A polymorphism,19%tested four polymorphisms(dPYd2A,HapB3,c.2846A>T,and dPYd13),and 66%tested five polymorphisms including *** Overall,14.8%of patients were found to be carriers of a dPYd variant,the most common being dPYd6(12.1%).Patients in cohort A reported≥G3 toxicities(P=0.00098),particularly fewer nonhematological toxicities(P=0.0028)compared with cohort B,whereas there was no statistically significant difference between the two cohorts in hematological toxicities(P=0.6944).Significantly fewer chemotherapy dose reductions(P=0.00002)were observed in cohort A compared to cohort B,whereas there was no statistically significant differences in chemotherapy *** Although this study had a limited sample size,it provides additional information on the prevalence of dPYd polymorphisms in the Italian population and highlights the role of pharmacogenetic testing to prevent severe toxicity.

关键词： dihydropyrimidine dehydrogenase dPYd polymorphisms Fluoropyrimidine Caucasian population Gastrointestinal cancers

Variable retention harvesting in Great Lakes mixed-pine forests: emulating a natural model in managed ecosystems

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Ecological Processes 2019年第1期8卷 210-224页

作者： Brian J.Palik Anthony W.d’amato USdA Forest Service Northern Research StationGrand RapidsMN 55744USA Rubenstein School of Environment and Natural Resources University of VermontBurlingtonVT 05095USA.

Variable retention harvesting(VRH)systems have gained wide use in many different forest types across the globe,but largely have been implemented in forests characterized by severe,infrequent disturbance *** has been less attention given to developing VRH approaches in forests that are characterized as having a mixed-severity disturbance regime that often results in only partial mortality of canopy trees in spatially heterogeneous *** example of such a forest type is red pine(Pinus resinosa Ait.)-dominated ecosystem of the western Great Lakes region of North *** purpose of this review is to provide a conceptual foundation for developing VRH approaches in red pine ecosystems that are based on a mixed-severity disturbance *** contention is that red pine forests managed following a natural model are more resilient to disturbances and external threats such as climate *** the red pine ecosystem,VRH application should reflect the often severe,but partial canopy removal from natural disturbance that is characteristic of this ecosystem and that results in more than trivial numbers of surviving overstory trees across a range of spatial configurations in regenerating *** live trees should span a range of diameters,but favor the larger end of the diameter distribution,as this reflects the likely pattern of survival after natural disturbance and is often a key structural element lacking from managed *** should be applied in ways that vary the spatial pattern of legacy trees in and among stands,but largely in ways that reflect the pattern of spatially patchy canopy structure,with large openings surrounded by a less disturbed matrix,as occurs with a natural disturbance *** trees and deadwood structures should reflect the composition of the pre-disturbance forest,including species in addition to dominant red ***,retained structures should be viewed as dynamic entities that grow,die,and decay and that need

关键词： Red pine Mixed-severity Variable retention Natural disturbance

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

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Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal J.Goldschmidt-Clermont Aisha Khan George Jimsheleishvili Patricia Graham Adriana Brooks Risset Silvera Alexander J.P.Goldschmidt damien d.Pearse W.dalton dietrich Allan d.Levi James d.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FdA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

dNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration

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Horticulture Research 2023年第1期10卷 183-192页

作者： Samaneh Najafi Edoardo Bertini Erica d’Incà marianna Fasoli Sara Zenoni department of Biotechnology University of Verona37134 VeronaItaly

CRISPR/Cas9 genome editing technology can overcome many limitations of traditional breeding,offering enormous potential for crop improvement and food *** the direct delivery of Cas9-single guide RNA(sgRNA)ribonucleoprotein(RNP)complexes to grapevine(Vitis vinifera)protoplasts has been shown before,the regeneration of edited protoplasts into whole plants has not been ***,we describe an efficient approach to obtain transgene-free edited grapevine plants by the transfection and subsequent regeneration of protoplasts isolated from embryogenic *** proof of concept,a single-copy green f luorescent protein reporter gene(GFP)in the grapevine cultivar Thompson Seedless was targeted and knocked out by the direct delivery of RNPs to ***/Cas9 activity,guided by two independent sgRNAs,was confirmed by the loss of GFP f *** regeneration of GFP−protoplasts into whole plants was monitored throughout development,confirming that the edited grapevine plants were comparable in morphology and growth habit to wild-type *** report the first highly efficient protocol for dNA-free genome editing in grapevine by the direct delivery of preassembled Cas9-sgRNA RNP complexes into protoplasts,helping to address the regulatory concerns related to genetically modified *** technology could encourage the application of genome editing for the genetic improvement of grapevine and other woody crop plants.

关键词： complexes fir editing

Biochemical dissection of STAT3 signaling in amyotrophic lateral sclerosis

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Neural Regeneration Research 2025年第11期20卷 3229-3230页

作者： Savina Apolloni Nadia d’Ambrosi department of Biology University of Rome Tor VergataRomeItaly

Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disease characterized by the loss of upper and lower motor neurons,clinically marked by muscle atrophy and *** the clinical course is highly variable,the average time from the onset of symptoms to the need for respiratory support or death is 3-5 *** is the most prevalent motor neuron disease in adults,occurring at a rate of 2 per 100,000 individuals and affecting 5.4 per 100,000 individuals overall.

关键词： STAT3 dissection sclerosis

Rapid dehydration of grape berries dampens the post-ripening transcriptomic program and the metabolite profile evolution

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Horticulture Research 2020年第1期7卷 1016-1030页

作者： Sara Zenoni Alessandra amato Erica d’Incà Flavia Guzzo Giovanni Battista Tornielli department of Biotechnology University of VeronaStrada Le Grazie 1537134 VeronaItaly

The postharvest dehydration of grape berries allows the concentration of sugars and other solutes and promotes the synthesis of metabolites and aroma compounds unique to high-quality raisin wines such as the passito wines made in *** dynamic changes are dependent on environmental parameters such as temperature and relative humidity,as well as endogenous factors such as berry morphology and genotype,but the contribution of each variable is not well ***,we compared berries subjected to natural or accelerated dehydration,the latter driven by forced air *** followed the evolution of transcript and metabolite profiles and found that accelerated dehydration clearly dampened the natural transcriptomic and metabolomic programs of postharvest *** found that slow dehydration over a prolonged duration is necessary to induce gene expression and metabolite accumulation associated with the final quality traits of dehydrated *** accumulation of key metabolites(particularly stilbenoids)during postharvest dehydration is inhibited by rapid dehydration conditions that shorten the berry life time.

关键词： flow dehydration latter