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Potassium channels as potential drug targets for limb wound repair and regeneration

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Precision Clinical Medicine 2020年第1期3卷 22-33页

作者： Wengeng Zhang Pragnya Das Sarah Kelangi marianna Bei Center for Engineering in Medicine Massachusetts General HospitalDepartment of SurgeryHarvard Medical SchoolBostonMA 02114USA Shriners Hospital for Children BostonMA 02114USA Center for Regenerative Developmental Biology The Forsyth InstituteCambridgeMA 02116USA Present address:Department of Pediatrics Division of NeonatologyDrexel University College of MedicinePhiladelphiaPA 19102USA

Background:Ion channels are a large family of transmembrane proteins,accessible by soluble membraneimpermeable molecules,and thus are targets for development of therapeutic *** channels are the second most common target for existing drugs,after G protein-coupled receptors,and are expected to make a big impact on precision medicine in many different diseases includingwound repair and *** has shown that endogenous bioelectric signaling mediated by ion channels is critical in non-mammalian limb ***,the role of ion channels in regeneration of limbs in mammalian systems is not yet ***:To explore the role of potassium channels in limb wound repair and regeneration,the hindlimbs of mouse embryos were amputated at E12.5 when the wound is expected to regenerate and E15.5 when the wound is not expected to regenerate,and gene expression of potassium channels was ***:Most of the potassium channels were downregulated,except for the potassium channel kcnj8(Kir6.1)which was upregulated in E12.5 embryos after ***:This study provides a new mouse limb regeneration model and demonstrates that potassium channels are potential drug targets for limb wound healing and regeneration.

关键词： limb regeneration wound healing drug targets potassium channels gene expression

Utilization of spent substrates and waste products of mushroom cultivation to produce new crops of Pleurotus ostreatus,Pleurotus eryngii and Agaricus bisporus

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Carbon Resources Conversion 2024年第2期7卷 50-63,F0004页

作者： marianna Dedousi Eirini-Maria Melanouri Dimitris Karayannis Eleftherios-Ikaros Kaminarides Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural Products(ITAP)Hellenic Agricultural OrganizationDimitra(ELGO-Dimitra)1 S.Venizelou str.14123 LykovryssiAttikiGreece Laboratory of Food Microbiology and Biotechnology Department of Food Science and NutritionAgricultural University of Athens75 Iera Odos Str.11855 AthensGreece Green Zin S.A.Agriculture 37008 PaliouriSourpiGreece

Five agro-industrial residues,their corresponding spent mushroom substrates(SMS),commercial fresh and spent mushroom compost of Agaricus(SMC)and Pleurotus ostreatus(SMS GZ)cultivation,Pleurotus and Agaricus waste(stipes/mishappen mushrooms)were combined and re-utilized as novel substrates for the cultivation of ***,*** and *** ***/SMC were used up to 40%(treatment 1 and 2),while 20%of mushroom waste were added in fresh and spent substrates(treatment 3 and 4).The impact of different substrates on mycelial growth rate and biomass production was ***,Pleurotus *** cultivated on the most promising substrates and essential cultivation aspects(earliness,total mushroom yield,biological efficiency-BE)and carposomes’quality parameters(weight,morphological characteristics)were *** and endoglucanase production by Pleurotus species were also determined at 50 and 100%of colonization *** species showed their fastest mycelial growth rate(up to 5 mm/day)on substrates consisted of SMC,whereas many combinations of species/substrate enhanced biomass *** SMS GZ supplementation positively affected laccase activity;in the cultures of *** and *** the highest values were 62,539 and 17,584 U/g d.w.,*** the contrary,small amounts of endoglucanase were produced(0.007 to 0.322 U/g d.w.);the greatest production was recorded for *** at full *** fermentation in bags,significant amount of total mushroom yield was produced in all substrates and those with SMS GZ supported the fastest earliness period and the highest BE for both Pleurotus *** values ranged from 54 to 133%for *** and from 53 to 121%for *** morphological characteristics,mushroom waste addition seemed to affect them *** data included in this paper support the effective re-utilization of different types of SMS and mushroom waste for fungal mass and enzymes’productio

关键词： Oyster mushroom Button mushroom Spent mushroom compost Stipes Misshapen mushrooms Glucosamine Laccase Endoglucanase Earliness Biological efficiency

Patient-derived organoids for therapy personalization in inflammatory bowel diseases

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World Journal of Gastroenterology 2022年第24期28卷 2636-2653页

作者： marianna Lucafò Antonella Muzzo Martina Marcuzzi Lorenzo Giorio Giuliana Decorti Gabriele Stocco Advanced Translational Diagnostics Laboratory Institute for Maternal and Child Health-IRCCS“Burlo Garofolo”Trieste 34137Italy Department of Medicine Surgery and Health SciencesUniversity of TriesteTrieste 34127Italy Department of Life Sciences University of TriesteTrieste 34127Italy

Inflammatory bowel diseases(IBDs)are chronic inflammatory disorders of the intestinal tract that have emerged as a growing problem in industrialized *** of IBD pathogenesis is still incomplete,and the most widely-accepted interpretation considers genetic factors,environmental stimuli,uncontrolled immune responses and altered intestinal microbiota composition as determinants of IBD,leading to dysfunction of the intestinal epithelial *** vitro models commonly used to study the intestinal barrier do not fully reflect the proper intestinal *** important innovation is represented by organoids,3D in vitro cell structures derived from stem cells that can self-organize into functional organ-specific *** may be generated from induced pluripotent stem cells or adult intestinal stem cells of IBD patients and therefore retain their genetic and transcriptomic *** models are powerful pharmacological tools to better understand IBD pathogenesis,to study the mechanisms of action on the epithelial barrier of drugs already used in the treatment of IBD,and to evaluate novel target-directed molecules which could improve therapeutic *** aim of this review is to illustrate the potential use of organoids for therapy personalization by focusing on the most significant advances in IBD research achieved through the use of adult stem cells-derived intestinal organoids.

关键词： Inflammatory bowel disease Organoids Intestinal epithelium 3D cell cultures Personalized medicine

Mechanisms and Prevention of Vertical Transmission in Chronic Viral Hepatitis

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Journal of Clinical and Translational Hepatology 2017年第2期5卷 119-129页

作者： marianna G.Mavilia George Y.Wu Department of Medicine Division of Gastroenterology-Hepatology University of Connecticut Health Center Farmington CT USA

Vertical transmission (VT) is the primary route of transmission of viral hepatitis in *** rate of VT ranges from 1-28% with hepatitis B virus (HBV) and 3-15% with hepatitis C virus (HCV).VT for both viruses can occur during the intrauterine or peripartum *** of HBV primarily occurs by intrauterine transmission (IUT).Hepatitis B surface antigen is unable to cross the placenta and,therefore,relies on processes like transplacental leakage,placental infection,cellular transmission by peripheral blood mononuclear cells,and germline *** can also infect the fetus by *** viruses also have the potential for transmission during delivery,when there is increase chance of maternal-fetal blood *** and HCV share some common risk factors for VT,including maternal viral load,human immunodeficiency virus co-infection and neonatal *** of VT differs greatly between HBV and *** are several alternatives for prevention of HBV VT,including antiviral medications during the third trimester of pregnancy and HBV vaccine,as well as hepatitis B immunoglobulin administration to infants *** contrast,there are no preventative interventions available for *** these differences,the key to prevention with both viruses is screening women prior to and during pregnancy.

关键词： Vertical transmission Hepatitis B Hepatitis C Mechanisms Prevention

Gastrointestinal motility disorders in inflammatory bowel diseases

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World Journal of Gastroenterology 2014年第1期20卷 37-44页

作者： Gabrio Bassotti Elisabetta Antonelli Vincenzo Villanacci marianna Salemme Manuela Coppola Vito Annese Gastroenterology and Hepatology Section Department of Clinical and Experimental MedicineUniversity of Perugia06156 PerugiaItaly Second Pathology Section Spedali Civili of Brescia25100 BresciaItaly Gastroenterology Unit Careggi University Hospital50134 FlorenceItaly

The relationship between motility and inflammatory gastrointestinal disorders is at the same time complex and intriguing since these conditions might share some genetic, environmental, immunological and microbial predisposing factors. In addition, significant symptom overlapping may occur, muddling the waters within the clinical context. Although on one hand this represents a challenge for the clinician for a potential under- or over-treatment and diagnostic delay, on the other hand it possibly represents an opportunity for the researcher to better disclose the intimate relationship between chronic (often low-grade) inflammation, motor disorders and deranged sensory function. The best example is probably represented by Crohn’s disease and ulcerative colitis. In fact, a number of gastrointestinal motor disorders have been described in association with these diseases, disorders which span from the esophagus to the anorectum, and which will be extensively covered in this review. It is conceivable that at least part of this derangement is strictly related to inflammatory cytokine trafficking and neuromuscular changes; however, given the high prevalence of functional gastrointestinal disorders in the general population, this overlap might also be serendipitous. However, it is worth noting that literature data on this topic are relatively scarce, sometimes quite outdated, and mostly focused on the interplay between irritable bowel syndrome and inflammatory bowel disease. Nevertheless, both researchers and clinicians must be aware that symptoms related to gastrointestinal motility disorders may be highly prevalent in both active and inactive inflammatory bowel disease, correlate with greater psychological comorbidity and poorer quality of life, and may negatively influence the therapeutic approaches.

关键词： Crohn’ s disease, Gastrointestinal motility disorders, Gut, Inflammatory bowel diseases, Perception, Ulcerative colitis

Differentiating Cystic Liver Lesions:A Review of Imaging Modalities, Diagnosis and Management

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Journal of Clinical and Translational Hepatology 2018年第2期6卷 208-216页

作者： marianna G.Mavilia Tina Pakala Marco Molina George Y.Wu Department of Medicine Division of Gastroenterology-HepatologyUniversity of Connecticut Health CenterFarmingtonCTUSA Department of Medicine Division of Gastroenterology-HepatologyNew York Presbyterian HospitalNew YorkNYUSA Department of Radiology University of Connecticut Health CenterFarmingtonCTUSA

Hepatic cysts(HCs)are frequently discovered incidentally on abdominal *** prevalence of HCs has been reported as high as 15–18%in the United *** most cysts are benign,some are malignant or *** is impor-tant to diagnose cystic lesions in order to properly manage *** with conventional ultrasound,computed to-mography,magnetic resonance imaging,or contrast-enhanced ultrasound can be used to further characterize and diagnose *** is typically the first-line imaging modality,whereas more advanced imaging can help narrow down the specific ***-enhanced ultrasound is a newer mo-dality,recently approved in the United States,which offers non-invasive evaluation in *** first step in diagnosis is stratifying risk by differentiating simple and complex *** are several features that can help identify HCs,including septae,mural consistency,calcifications,and quality of cystic *** cysts are mainly congenital cysts,but also occur in polycystic liver *** cysts include mucinous neoplasms,echinococcal cysts,hemorrhagic cysts,cystic hep-atocellular carcinoma and other rare *** is indicated in symptomatic cysts or those suspicious for malig-nant or premalignant *** modalities include fenestration,aspiration sclerotherapy,or surgical resection.

关键词： Hepatic cyst Simple cyst Complex cyst Hepatic imaging Contrast-enhanced ultrasound Treatment

Associations between cardiac arrhythmia,incident disability in activities of daily living and physical performance:the ILSA study

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Journal of Geriatric Cardiology 2020年第3期17卷 127-132,I0002-I0004页

作者： marianna Noale Nicola Veronese Lee Smith Andrea Ungar Stefano Fumagalli Stefania Maggi the Italian Longitudinal Study on Aging Working Group National Research Council(CNR) Neuroscience InstituteAging BranchVia Giustiniani 235128 PaduaItaly Azienda ULSS 3 Serenissima Primary Care DepartmentDistrict 3VeniceItaly The Cambridge Centre for Sport and Exercise Sciences Department of Life SciencesAnglia Ruskin UniversityCambridge CB11PTUK Geriatrics and Intensive Care Unit Department of Experimental and Clinical MedicineUniversity of Florence and AOU CareggiFlorenceItaly 不详

Background Cardiac arrhythmias are common conditions in older ***,there is limited literature on associations between cardiac arrhythmias and physical performance or *** therefore aimed to prospectively investigate associations between cardiac arrhythmias and changes in disability and physical performance during 8 years of follow-up,using data from the Italian Longitudinal Study on Aging(ILSA).Methods Cardiac arrhythmias diagnosis was posed through a screening phase,confirmed by a *** onset of disability in activities of daily living(ADL)and the changes in several physical performance tests during follow-up were considered as ***-adjusted and propensity-score Cox Proportional Hazard models and mixed models were used for exploring associations between cardiac arrhythmia and the outcomes of *** The prevalence of cardiac arrhythmia at baseline was 23.3%.People reporting cardiac arrhythmia at the baseline were significantly older,more frequently male,smokers and reported a higher presence of all medical conditions investigated(hypertension,heart failure,angina,myocardial infarction,diabetes,stroke),but no difference in dementia,Parkinsonism,cognitive or mood *** arrhythmia at baseline was significantly associated with the incidence of disability in ADL(HR=1.23;95%:CI:1.01–1.50;P=0.0478 in propensity score analyses;HR=1.28;95%CI:1.01–1.61;P=0.0401 in fully adjusted models).Cardiac arrhythmia at baseline was also associated with a significant worsening in balance test(P=0.0436).Conclusions The presence of cardiac arrhythmia at baseline was associated with a significant higher risk of disability and of worsening in some physical performance tests,particularly those relating to *** and frequently assessing physical performance in older people affected by cardiac arrhythmia can be important to prevent a loss of physical performance,with further,potential,complications of medical managemen

关键词： Atrial fibrillation Cardiac arrhythmia Disability Physical activity Physical performance

Short-term solar eruptive activity prediction models based on machine learning approaches:A review

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Science China Earth Sciences 2024年第12期67卷 3727-3764页

作者： Xin HUANG Zhongrui ZHAO Yufeng ZHONG Long XU marianna B.KORSÓS R.ERDÉLYI Faculty of Electrical Engineering and Computer Science Ningbo UniversityNingbo 315211China State Key Laboratory of Space Weather National Space Science CenterChinese Academy of SciencesBeijing 100190China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China Beijing Cellular Exploration Science and Technology Center Beijing 100190China Dipartimento di Fisica e Astronomia“Ettore Majorana” Universitàdi CataniaCatania I 95123Italy Department of Astronomy Eötvös Loránd UniversityBudapest H-1112Hungary Hungarian Solar Physics Foundation Gyula H-5700Hungary Solar Physics&Space Plasma Research Center(SP2RC) School of Mathematics and StatisticsUniversity of SheffieldSheffield S37RHUK

Solar eruptive activities,mainly including solar flares,coronal mass ejections(CME),and solar proton events(SPE),have an important impact on space weather and our *** short-term solar eruptive activity prediction is an active field of research in the space weather ***,statistical,and machine learning methods are proposed to build prediction models of the solar eruptive *** the development of space-based and ground-based facilities,a large amount of observational data of the Sun is accumulated,and data-driven prediction models of solar eruptive activities have made a significant *** this review,we briefly introduce the machine learning algorithms applied in solar eruptive activity prediction,summarize the prediction modeling process,overview the progress made in the field of solar eruptive activity prediction model,and look forward to the possible directions in the future.

关键词： Solar flare Coronal mass ejection Solar proton event Machine learning Prediction model

Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa

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Asian Journal of Andrology 2017年第4期19卷 409-413页

作者： Donatella Paoli marianna Pelloni Mariagrazia Gallo Giulia Coltrinari Francesco Lombardo Andrea Lenzi Loredana Gandini Laboratory of Seminology - Sperm Bank Department of Experimental Medicine University of Rome 'La Sapienza' 00161 Rome Italy

It has been suggested that the energy required for sperm motility is produced by oxidative phosphorylation while glycolysis seems to be an important source for ATP transmission along the flagellum. Some studies have investigated the chemical and kinetic properties of the enzyme glyceraldehyde 3-phosphate dehydrogenase to identify any changes in the regulation of glycolysis and sperm motility. In contrast, there are few studies analyzing the genetic basis of hypokinesis. For this reason, we investigated the glyceraldehyde 3-phosphate dehydrogenase gene in human sperm to evaluate whether asthenozoospermia was correlated with any changes in its expression. Semen examination and glyceraldehyde 3-phosphate dehydrogenase gene expression studies were carried out on 116 semen samples divided into two groups - Group A consisted of 58 normokinetic samples and Group B of 58 hypokinetic samples. Total RNA was extracted from spermatozoa, and real-time PCR quantification of mRNA was carried out using specific primers and probes. The expression profiles for the Groups A and B were very similar. The mean delta Ct was as follows - Group A, 5.79 ＋ 1.04; Group B, 5.47 ＋ 1.27. Our study shows that in human sperm, there is no difference in glyceraldehyde 3-phosphate dehydrogenase gene expression between samples with impaired motility and samples with normal kinetics. We believe that this study could help in the understanding of the molecular mechanisms of sperm kinetics, suggesting that hypomotility may be due to a possible posttranscriptional impairment of the control mechanism, such as mRNA splicing, or to posttranslational changes.

关键词： adenosine-5'-triphosphate gene expression sperm glyceraldehyde 3-phosphate dehydrogenase sperm motility