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Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa

Asian Journal of Andrology 2017年第4期19卷 409-413页

作者： Donatella Paoli marianna pelloni Mariagrazia Gallo Giulia Coltrinari Francesco Lombardo Andrea Lenzi Loredana Gandini Laboratory of Seminology - Sperm Bank Department of Experimental Medicine University of Rome 'La Sapienza' 00161 Rome Italy

It has been suggested that the energy required for sperm motility is produced by oxidative phosphorylation while glycolysis seems to be an important source for ATP transmission along the flagellum. Some studies have investigated the chemical and kinetic properties of the enzyme glyceraldehyde 3-phosphate dehydrogenase to identify any changes in the regulation of glycolysis and sperm motility. In contrast, there are few studies analyzing the genetic basis of hypokinesis. For this reason, we investigated the glyceraldehyde 3-phosphate dehydrogenase gene in human sperm to evaluate whether asthenozoospermia was correlated with any changes in its expression. Semen examination and glyceraldehyde 3-phosphate dehydrogenase gene expression studies were carried out on 116 semen samples divided into two groups - Group A consisted of 58 normokinetic samples and Group B of 58 hypokinetic samples. Total RNA was extracted from spermatozoa, and real-time PCR quantification of mRNA was carried out using specific primers and probes. The expression profiles for the Groups A and B were very similar. The mean delta Ct was as follows - Group A, 5.79 ＋ 1.04; Group B, 5.47 ＋ 1.27. Our study shows that in human sperm, there is no difference in glyceraldehyde 3-phosphate dehydrogenase gene expression between samples with impaired motility and samples with normal kinetics. We believe that this study could help in the understanding of the molecular mechanisms of sperm kinetics, suggesting that hypomotility may be due to a possible posttranscriptional impairment of the control mechanism, such as mRNA splicing, or to posttranslational changes.

关键词： adenosine-5'-triphosphate gene expression sperm glyceraldehyde 3-phosphate dehydrogenase sperm motility

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Inhibin B:are modified ranges needed for orchiectomised testicular cancer patients?

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Asian Journal of Andrology 2019年第4期21卷 332-336页

作者： Alessandra Petrozzi Francesco Pallotti marianna pelloni Antonella Anzuini Antonio Francesco Radicioni Andrea Lenzi Donatella Paoli Francesco Lombardo Laboratory of Seminology Sperm Bank "Loredana Gandini" Department of Experimental Medicine Medical Pathophysiology Section"Sapienza" University of Rome Rome 00161 Italy Hormone Laboratory Department of Experimental Medicine Medical Pathophysiology Section"Sapienza" University of Rome Rome 00161 Italy

Inhibin B is a gon adal horm one that down regulates the pituitary production of follicle-stimulati ng horm one (FSH). In recent years, inhibin B has proved to be an excellent marker of spermatogenesis and even a predictive factor for the recovery of fertility in patients undergoing orchiectomy and antineoplastic treatments. We propose to study inhibin B levels in orchiectomised testicular cancer patients, in order to identify a minimum value representative of normal semen quality. This retrospective study evaluates horm onal and semen parameters of 290 no rmozoospermic patie nts atte nding the Laboratory of Semi no logy - Sperm Bank "Loredana Gandini"(Rome, Italy) for cryopreservation of seminal fluid following a diagnosis of testicular cancer (TC group) and 117 healthy, normozoospermic men as a control group (CTR group). The percentile distribution of gonadotropin and inhibin B values in the TC and CTR groups was analyzed. There was a statistically significant differenee between the two groups in the levels of all hormones (P ≤ 0.001) and in all semen parameters (P 95^th percentile of CTR group. Orchiectomised patients for testicular cancer presented inhibin B levels lower than healthy patients, despite normozoospermia. Our study revealed the poor sensitivity of the current inhibin B reference range when applied to monorchidic patients, suggesting the need to establish more representative ranges to enable more appropriate counseling in relation to the patienfs new endocrine condition.

关键词： inhibin B orchiectomy referenee range testicular cancer

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